FastQCFastQC Report
Sat 31 Dec 2022
SRR5846838.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846838.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187881
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAAGGTCCATCTCGTATGCCGTCT15660.8335063151675796RNA PCR Primer, Index 16 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAAGGTCCATCTCGTATGC14360.7643135814691214No Hit
GTTATCGGGCTCGGCAGTGGTACCTGTTCCCGGTGTCCCTGAGGACGTGCGG2660.1415789781829988No Hit
CAGCAAGGGCATCACCTCGGTCGTCCCTTCCCACCAGGCTACTGTGCTACTC2620.13944997099227702No Hit
GCTTCGGGGTATTTTGTGAAGATCTTAAATTTGTGGTATAAGAACTTGAAGG2540.13519195661083344No Hit
GACAATCTTGGCTCTGATGAAGACCCCAGTCCTCTTTGACATTTATGAGATA2480.1319984458247508No Hit
GGATGTGGGTGCTTCTGGCCCCAAAGTTGTAAAACACATATGTATGGCATAT2480.1319984458247508No Hit
ACCGTCGGGGTCCTTTTGAATTAAGAGATTTAGTTATGTTTACTAAGAATGT2460.13093394222938987No Hit
GTTTATGGGAAGATTGAGCCTCCGCTGATTTTCGAGTGTAACCAGGCGTGCT2460.13093394222938987No Hit
ACAGACGGGCTTGCTAACAAATTCTTCCTAATAGAGGTCCTGTTAGAGTCAA2380.12667592784794632No Hit
CCGCACGGGGAACCTTTTCAGTAGGGAAATTCAGAAAATGGAATGATTCATC2380.12667592784794632No Hit
CAATATGGGGATAACTAAAAATTGGACATAAGAGAGACAGTGTCCAGGAAAG2370.12614367605026586No Hit
CCAACGGGATGAGGGAATTCCGGTCAGGGTCAAGTCGTGTTCTGATCACTAC2350.12507917245490496No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAGGTCCATCCTGTCTCTTATAC2350.12507917245490496No Hit
TTAGTAGGGGATCTCATGACGGGAGCAGAGGATTCCTCTGGGCCGGTGATTT2280.12135340987114185No Hit
ACTTAGGGGCTGAAAAGGGAGGAGTATTTTTAGTTTGACAATTTAATAATTT2280.12135340987114185No Hit
TCGGGCGGGTAAGAAACACAATTAATGTATATGAAACAGCGACATCCACAGA2240.11922440268042005No Hit
TTTGACGGGTATTTTATGGTACATTTTGAAAAGAAAATTTGTAATGCTCCTT2240.11922440268042005No Hit
TGCTGCGGGTTAAAGATAAATTTAAGAAGACTCAAAATAGAAAGGATACAGA2230.11869215088273961No Hit
ATTCAAGGGAGGTGAGGCAGGCATTTTTCTATTCCCTTAACATGACCAGTTG2220.11815989908505915No Hit
ATTGTCGGGGACGCGCACGAACGCACGGCCGCGCAGCATCTGTCTTGCTGGA2210.11762764728737872No Hit
TGCGCTGGGAGGGACCAATCCTGGAGAGATAGAGATTTGCGGCCTTTCAGAC2200.11709539548969826No Hit
TAGTCGGGGGTTGAAGTGGTTTTTTGTTTTTGTTTTTGTTTTTTTAATTTGT2200.11709539548969826No Hit
TTCATAGGGAGGAGAAGGAGGAGAAGGAGCGGAGGCGCGAGCGGGCCGACCG2200.11709539548969826No Hit
TCTACAGGGGCTTTTCCTCCGCTGGAGCAGCTATGGCGGCGGTGAAGACCCT2160.11496638829897649No Hit
CAAGTAGGGGAATATAGTATGTTATAAGTAGTGGTTACTAGAGAGCAGATGA2160.11496638829897649No Hit
TGGCATGGGAATTGGGCATGGTGGTGGACACCTGTAATCCCAAAATGTTCAG2150.11443413650129602No Hit
GCATGTGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGT2150.11443413650129602No Hit
AGCAGGGGGCAGCGAATCAAGCGGGAGTTCAAGGAGGTGCTGAAGAGCGAGG2150.11443413650129602No Hit
GTCGACGGGCAGCTGAGAGTGAAGCAAAAGTAAAAACCAAAGTTCGCTTTGA2140.11390188470361559No Hit
GCTATAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA2130.11336963290593514No Hit
GTCTGCGGGACTATGTCTCAAGGAGAGATTACCAAGTTGGCAGTGAGACAGA2130.11336963290593514No Hit
TCTAAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2120.11283738110825468No Hit
GGGTACGGGAACATACATTGCTTGGCCAAAGCCATCAACCAGATTGCTGCAG2120.11283738110825468No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAAGGTCCATCCTGTCTCT2120.11283738110825468No Hit
CTCTCAGGGTACCTGGTACTGTGGCACCTCCCAGAAAGTCTGGTTTTTGCCC2120.11283738110825468No Hit
GCGACGGGGCTGCTGCTTTCCTTCGGAATCCAGTGCTTCCACAGAGATTAGC2110.11230512931057425No Hit
CTTCAAGGGATTGGCAACAAATACTGTTATTTTCCTTAACATGACAGGCTCA2110.11230512931057425No Hit
ACTTGTGGGTTGCTAGATAACCACATATTCCACTTAGAGATTAGCATACATC2100.11177287751289379No Hit
CGTGGCGGGTAACATAAAGCAAGTGAGCGACCTTATCTCTGTGCTTCGTGAA2100.11177287751289379No Hit
ACTTACGGGAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATC2070.11017612211985248No Hit
GGTACCGGGCAAGAAGAAAGTGAGGAAGTTGGAAGATCAGAACGAGTATGAA2050.10911161852449155No Hit
AGCATTGGGCACTGTGCCCAGCCCAAAGACCTATTTTTAATAGATATTTTAG2050.10911161852449155No Hit
AACACAGGGATTTTGCTAATAATGTTAGGCATCTAATCATCTAGAGCTGTCC2040.10857936672681112No Hit
CTTTGCGGGATAACATAAAAAACACATCACTATATGTAGGAAATATACATTT2040.10857936672681112No Hit
ACTCAGGGGCCCTGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACG2030.10804711492913067No Hit
GCTTTCGGGATCCCTGTTTCACTCCCAAACCCTCTCAAATTCATTTCTTCAC2030.10804711492913067No Hit
GCTGCAGGGCAGCAAAAACAGGAGAATCACATGCTTTAAAGAACACTTTCTC2020.10751486313145024No Hit
GTTTGTGGGATGGAGCTTCTTTGAGGCTTGAAGTTTGATTTTACTATCTAGA2010.10698261133376978No Hit
GACTTGGGGAAGACATGGAAGTTGGGAGGCACTGAATCAATGAAACTGCCAA2010.10698261133376978No Hit
CTCATTGGGGGCCACGTGATCTTATTTTAAAAGAACACTGCTATAGAAGAAT2010.10698261133376978No Hit
TCAACTGGGATTAACTTTCTGGTCTTTTATGTATTCCAGAAACTGGGAGTGA2000.10645035953608932No Hit
CTCTATGGGAAGAGTGATGTGGTGCCACTGAAGAGTTTTAAGCTGCTAGCTG2000.10645035953608932No Hit
TTACATGGGATGAAAGTTTAGGAAATTTGGCTATTGAGTATATTAAGTACAA1990.10591810773840889No Hit
CCGCGAGGGCGAACGAAAAATTCTAGGCTATATACAACTACGCAAAGGCCCC1990.10591810773840889No Hit
GCAGTGGGAACATGTTGCTTTGTATAAAAATCTTATTTATAAATGTGAAGCT1990.10591810773840889No Hit
AGCCTTGGGAACACTTCACAACATGGAACTAACAGACATCTACAGAACAATC1980.10538585594072844No Hit
CATCACGGGACCATCCTGTCTCGCCGTATTGCTGTTGAATATAGTGATTCGG1980.10538585594072844No Hit
AAGCGTGGGGAGAGAAACAACAAAATACTCTCGTTGTTGTTCTTGGATCCAA1980.10538585594072844No Hit
TCGCACAGGGTCCAGCAAAATGAACTTAAAAAGATGTTTATACAATGCCAGA1980.10538585594072844No Hit
AATGGGGGGTGACAACCCTACAGATGGTTTAATTTAGCTTGACCCTGATGGA1970.10485360414304801No Hit
AAAGCAGGGGCCTCGAGTGGAGGAGTCTGTTCCTTTTGTTCCTCCCTCGATT1970.10485360414304801No Hit
CACACAGGGAGGCAATGAACTCGCTCTTCAAATACCATAGTTCAAGAGTAGC1960.10432135234536755No Hit
GCACTTGGGCTATAACATGTCTTTCAGCATTAGGCTTTTAAGAAACACAGGG1950.10378910054768709No Hit
CGCATAGGGATATACAAAGCTAAAAAGATAGTTAGCACCATTGCAATCAATA1950.10378910054768709No Hit
ATTTCTGGGCCTGAAACTACACACCATTTCTTCCCTGCTCAGCTTCTGCTCA1940.10325684875000665No Hit
ACGACCGGGAACTGAGTTATATTCACTGTGGCTCAAACTAATTGGCATTGTG1930.1027245969523262No Hit
CTATTTGGGACAGCTGTGAAGAGAAAGTTTCCCAGTGAGTTAATAAGTTGGC1930.1027245969523262No Hit
AGTAAGGGAAATCATAAACCTTGGCTTTCATGGTTTACATTATTTTGTAAAT1920.10219234515464577No Hit
GAGCTAGGGCTCTTGGGATATGGGGGCTTAGGAAAACAATCCAACTCCCTAG1920.10219234515464577No Hit
TTGTGGGGGTAAGACAGGACTTTTTAAATTTACAATATTTTTGTCCTCAAGT1920.10219234515464577No Hit
TCAGCCGGGTTATACATGCAAAATAGAAAAAAAATGTTCAACATTTATTGAT1910.10166009335696531No Hit
GCGATAGGGGCCAGGTGATGCAAGGCATGGGGGTGTCTGCTGCCCAGGCCAC1910.10166009335696531No Hit
AGGTACGGGATTGTCAAGTTGTTTTATGTACATTAACTCATTGAATAATTAC1910.10166009335696531No Hit
ACACGTGGGATAACAGAATTCCTCTCTTCTCCAACTCGTTTTATATATGCTG1910.10166009335696531No Hit
CGCTGGGGGGGCCCCAGTCACTGAGCCGCCGCCGAGGATTCAGCAGCCTCCC1910.10166009335696531No Hit
CGCTATGGGACATATTTCAAAGTTAGATATGTGTCTTTGAATATATTTAGAA1910.10166009335696531No Hit
GTAAAGGGGATGACAGAACAGTGATCACATATATTTGGATTTATATGATATA1910.10166009335696531No Hit
CTTGCAGGGATAGTTACTGTCTTTCTGTGGTCTTAAGAAAAATTCAGAAACA1900.10112784155928488No Hit
AAAATCTACTGCTCTTTCTGAATCTCCAGATATCATTTCTGTATGGTTTCTG1900.10112784155928488No Hit
ATGGCTGGGGGGCTCTTTCTGACTCTGCTCATGAGATGGTATCAGCCACCCA1900.10112784155928488No Hit
GGTGCTGGGGCCCTGACTGCAGAGGGTCAGGGGCTCCTGCCCCAGTGCCTGC1900.10112784155928488No Hit
AAAATAGGGATATTTGAAAAGGCTTCCATTAATATAATAGCTTTGCTTGCAG1900.10112784155928488No Hit
CTTGTTGAGGGATATTTATTCATCCTTAATTGTATTCAGATTTTTTTTTAAG1900.10112784155928488No Hit
GTTTGTGGGATTTCTTGCCTGTCCGAACGTTGTATAAACGTGAAGAAAAATA1900.10112784155928488No Hit
CCATTAGGACTGGTTATTGTAATAAACCACCCATGGTTCTTTATGTACATTT1900.10112784155928488No Hit
TTGCTGGGAGCAGTACCTGCCCCGTCCAAGGAGAGAACCGGTTCCGTCCCCA1900.10112784155928488No Hit
TTCGTTGGGTTATGCAAAAAGTCTTTGCTTTGGCCTGGAGCATCTCCCCAAA1890.10059558976160442No Hit
ACAGAAGGGTGTTGAGGACTGGGACTTAGTTACCAGGCAGAAGCAGCTGTTT1890.10059558976160442No Hit
CATGCTGGGATTGCAGGTGTGAGCCGCCATGCCCGGCCATGTAGACAGTTAA1890.10059558976160442No Hit
GAAGGGGGGAGTGAGCGGCGGGGTCGACTGACGGTAACGGGGCAGAGAGGCT1890.10059558976160442No Hit
GTTCGCGGGATATGCAGCTGGGAGAACTACACCTTTGTGCACATAGATTTAT1880.10006333796392397No Hit
TTAGTAGGGGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGCACCTCGAAA1880.10006333796392397No Hit
GTGCAGGGGAGAAGGACTTGGAGGACCTGTTCTACAAGTACGGCCGCATCCG1880.10006333796392397No Hit
GCAAGCGGGCTGCCTGAACATTTGCCGCTTAGACTTTAGCTTCCCAGATGAC1880.10006333796392397No Hit
ATTGTTGGGACCAATCAGAATTCGAAGGATTTTCCTTTGTTAACTCTGAATT1880.10006333796392397No Hit
ACTGTAGGGACGTATCCTATATTTTGAATTTTATACTAGTTTTTCAAAAGTA1880.10006333796392397No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)