FastQCFastQC Report
Sat 31 Dec 2022
SRR5846837.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846837.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186459
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAAGGTCCATCTCGTATGCCGTCT15560.8344998096096192RNA PCR Primer, Index 16 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAAGGTCCATCTCGTATGC13680.733673354463984No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAGGTCCATCCTGTCTCTTATAC2660.14265870781244133No Hit
GCTTCGGGGTATTTTGTGAAGATCTTAAATTTGTGGTATAAGAACTTGAAGG2550.1367592875645584No Hit
ACAGACGGGCTTGCTAACAAATTCTTCCTAATAGAGGTCCTGTTAGAGTCAA2500.13407773290642985No Hit
GACTTGGGGAAGACATGGAAGTTGGGAGGCACTGAATCAATGAAACTGCCAA2460.13193248917992695No Hit
CAATATGGGGATAACTAAAAATTGGACATAAGAGAGACAGTGTCCAGGAAAG2450.13139617824830124No Hit
TGCTGCGGGTTAAAGATAAATTTAAGAAGACTCAAAATAGAAAGGATACAGA2400.12871462359017263No Hit
CGTGGCGGGTAACATAAAGCAAGTGAGCGACCTTATCTCTGTGCTTCGTGAA2380.1276420017269212No Hit
TTAGTAGGGGATCTCATGACGGGAGCAGAGGATTCCTCTGGGCCGGTGATTT2370.1271056907952955No Hit
AATGGGGGGTGACAACCCTACAGATGGTTTAATTTAGCTTGACCCTGATGGA2320.12442413613716688No Hit
GTTTATGGGAAGATTGAGCCTCCGCTGATTTTCGAGTGTAACCAGGCGTGCT2310.12388782520554116No Hit
TCTACAGGGGCTTTTCCTCCGCTGGAGCAGCTATGGCGGCGGTGAAGACCCT2300.12335151427391544No Hit
ACCGTCGGGGTCCTTTTGAATTAAGAGATTTAGTTATGTTTACTAAGAATGT2290.12281520334228972No Hit
GTCTGCGGGACTATGTCTCAAGGAGAGATTACCAAGTTGGCAGTGAGACAGA2240.12013364868416113No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAAGGTCCATCCTGTCTCT2230.1195973377525354No Hit
GTTATCGGGCTCGGCAGTGGTACCTGTTCCCGGTGTCCCTGAGGACGTGCGG2210.11852471588928398No Hit
CGCTGGGGGGGCCCCAGTCACTGAGCCGCCGCCGAGGATTCAGCAGCCTCCC2190.11745209402603254No Hit
AAAATAGGGATATTTGAAAAGGCTTCCATTAATATAATAGCTTTGCTTGCAG2170.1163794721627811No Hit
GCGACGGGGCTGCTGCTTTCCTTCGGAATCCAGTGCTTCCACAGAGATTAGC2130.11423422843627822No Hit
TGGCATGGGAATTGGGCATGGTGGTGGACACCTGTAATCCCAAAATGTTCAG2120.1136979175046525No Hit
GGTACCGGGCAAGAAGAAAGTGAGGAAGTTGGAAGATCAGAACGAGTATGAA2100.11262529564140106No Hit
GTAAAGGGGATGACAGAACAGTGATCACATATATTTGGATTTATATGATATA2090.11208898470977534No Hit
GAGCAAGGGCAGTACTAATTTCTCTCACTTAATTCCTTCTGTTTTACTCTTT2080.11155267377814962No Hit
AGCAGGGGGCAGCGAATCAAGCGGGAGTTCAAGGAGGTGCTGAAGAGCGAGG2070.1110163628465239No Hit
GCAGTGGGAACATGTTGCTTTGTATAAAAATCTTATTTATAAATGTGAAGCT2070.1110163628465239No Hit
TGGAGGGACAGGTAAATCTGTTCTTGCAAAGATGTGAGCAGAATAGTTAAAA2050.10994374098327246No Hit
CAGCAAGGGCATCACCTCGGTCGTCCCTTCCCACCAGGCTACTGTGCTACTC2050.10994374098327246No Hit
TCGCACAGGGTCCAGCAAAATGAACTTAAAAAGATGTTTATACAATGCCAGA2050.10994374098327246No Hit
CCGCACGGGGAACCTTTTCAGTAGGGAAATTCAGAAAATGGAATGATTCATC2040.10940743005164674No Hit
GAGCTAGGGCTCTTGGGATATGGGGGCTTAGGAAAACAATCCAACTCCCTAG2040.10940743005164674No Hit
TTTGACGGGTATTTTATGGTACATTTTGAAAAGAAAATTTGTAATGCTCCTT2040.10940743005164674No Hit
GGATGTGGGTGCTTCTGGCCCCAAAGTTGTAAAACACATATGTATGGCATAT2040.10940743005164674No Hit
ATGCAGGGGTGCTCAGAATATCATATAATGTTTATTTTTTGAGGTGTTTTTG2030.10887111912002102No Hit
CCGCGAGGGCGAACGAAAAATTCTAGGCTATATACAACTACGCAAAGGCCCC2030.10887111912002102No Hit
TCAGCCGGGTTATACATGCAAAATAGAAAAAAAATGTTCAACATTTATTGAT2030.10887111912002102No Hit
ACTGTAGGGACGTATCCTATATTTTGAATTTTATACTAGTTTTTCAAAAGTA2030.10887111912002102No Hit
AAAATCTACTGCTCTTTCTGAATCTCCAGATATCATTTCTGTATGGTTTCTG2000.10726218632514387No Hit
GATCACGGGTCTTGGGAATTTTGTATACATTATGGAATATTAGGATAAGTTT2000.10726218632514387No Hit
CTTTGCGGGATAACATAAAAAACACATCACTATATGTAGGAAATATACATTT2000.10726218632514387No Hit
GACAATCTTGGCTCTGATGAAGACCCCAGTCCTCTTTGACATTTATGAGATA1990.10672587539351813No Hit
CTCTATGGGAAGAGTGATGTGGTGCCACTGAAGAGTTTTAAGCTGCTAGCTG1990.10672587539351813No Hit
ACTTAGGGGCTGAAAAGGGAGGAGTATTTTTAGTTTGACAATTTAATAATTT1990.10672587539351813No Hit
AGCATTGGGCACTGTGCCCAGCCCAAAGACCTATTTTTAATAGATATTTTAG1980.10618956446189241No Hit
CGATAAGGGTCCCAATTGGCTTCTTTGTACAAATTGACAAGCTGCTCCTAAA1970.1056532535302667No Hit
AGGTACGGGATTGTCAAGTTGTTTTATGTACATTAACTCATTGAATAATTAC1970.1056532535302667No Hit
GCTGCAGGGCAGCAAAAACAGGAGAATCACATGCTTTAAAGAACACTTTCTC1970.1056532535302667No Hit
GCCCATGGGCTTGTGATGGGACAGAAATAAACCTCTTACAGAGTCAACAGGA1950.10458063166701526No Hit
GCAAGCGGGCTGCCTGAACATTTGCCGCTTAGACTTTAGCTTCCCAGATGAC1940.10404432073538954No Hit
ACTTACGGGAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATC1940.10404432073538954No Hit
ATTCAAGGGAGGTGAGGCAGGCATTTTTCTATTCCCTTAACATGACCAGTTG1940.10404432073538954No Hit
CCAGCCATGGGGGATAAAATATATGGACTGAATTGTGAGAATATAAATGCAT1940.10404432073538954No Hit
ACGACCGGGAACTGAGTTATATTCACTGTGGCTCAAACTAATTGGCATTGTG1930.10350800980376385No Hit
CTTGTTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1930.10350800980376385No Hit
ACAGAAGGGTGTTGAGGACTGGGACTTAGTTACCAGGCAGAAGCAGCTGTTT1920.10297169887213813No Hit
CATGCTGGGATTGCAGGTGTGAGCCGCCATGCCCGGCCATGTAGACAGTTAA1920.10297169887213813No Hit
ACTCAGGGGCCCTGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACG1910.1024353879405124No Hit
TACTGTGGGGATGAAAGAAAGGAAGGGAGAGAGGAAGAAAAAACAACCAGAA1910.1024353879405124No Hit
CTATTTGGGACAGCTGTGAAGAGAAAGTTTCCCAGTGAGTTAATAAGTTGGC1910.1024353879405124No Hit
CTTGTTGAGGGATATTTATTCATCCTTAATTGTATTCAGATTTTTTTTTAAG1910.1024353879405124No Hit
ACACGTGGGATAACAGAATTCCTCTCTTCTCCAACTCGTTTTATATATGCTG1890.10136276607726095No Hit
AGCTTGGGTGAGCTAGCATAGTCTTTCAAATGTAAATATTTCAATATTTGCT1890.10136276607726095No Hit
CGCTATGGGACATATTTCAAAGTTAGATATGTGTCTTTGAATATATTTAGAA1890.10136276607726095No Hit
AAAAGGGAGGCTATGCATTAATCGAAGCTTTCTTTTCACCATGACTTTATAA1880.10082645514563524No Hit
TTTATGGGGAAGGGTGGTGGACTATCTTGAAGAACCCTTTCAACTCTAAAAA1880.10082645514563524No Hit
GCTTGAGGGGTCACATGGGATGAATTCAATTTTAATATTTTTGAGAAAAGTC1880.10082645514563524No Hit
TAGTCGGGGGTTGAAGTGGTTTTTTGTTTTTGTTTTTGTTTTTTTAATTTGT1880.10082645514563524No Hit
TGCTCTCGGGACCACCTTCAACTCCATCATGAAGTGTGACGTGGACATCCGC1870.10029014421400952No Hit
GTGTGCAGGGAGGCGCCCGGTGGCTCCGAAGCGGGGAAGTGGGACAAGATGG1870.10029014421400952No Hit
CTGGATGGATTAAAGAAACCACAGGGATTTCTGCTCTTGTGTAGTTGTGTAT1870.10029014421400952No Hit
GAATCAGGACGCAAAACTAACCCCCTAATAAAATTAATTAACCACTCATTCA1870.10029014421400952No Hit
TTGTCTGGGAGATGAAGACATTAATAAAAGGTAAAATTATTTTTACATTTGA1870.10029014421400952No Hit
CTACATGGGAATTGTCTAAAAGGTAACTGATTTCATCAAACCTTCCAGTATT1870.10029014421400952No Hit
CCTGGGATAAGAAGATAGTTGCAGTTAAATTTTGAAAATGGTTGTAATGAGC1870.10029014421400952No Hit
TTGCTTGGGAAGGGGGTTGCTCCTCTCTGGATGAGACAGATACCATACACCA1870.10029014421400952No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)