FastQCFastQC Report
Sat 31 Dec 2022
SRR5846827.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846827.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237751
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAAGGTCCATCTCGTATGC7790.32765372175090746No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAGGTCCATCTCGTATGCCGTCT7210.3032584510685549RNA PCR Primer, Index 16 (95% over 21bp)
AACGTAGGGAGACGCGCCGGAACCGGGACGCGATAAATATGCAGAGCGGAGG2950.1240793939878276No Hit
GTTGTTGGGAAACAGATGGAAGAAGAAAAGCAGCAAACAGAAAGGGTTACAA2930.12323817775740165No Hit
GAAACTGGGAACAGAAAAATTGCCACATGAGTCAAATAGCATAGGTTAGAAA2710.1139847992227162No Hit
ACATCGGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGT2520.10599324503366968No Hit
AGCCTGGGTGATAGAGCAAGATCCTGCCTTTAAAAAAAGAGAAAGGAAAATC2480.10431081257281777No Hit
GCGAAAGGGAATAAATAACCTTTTTCTATAAATATCTTGATTAGATTCAGTG2470.10389020445760482No Hit
CACGCGGGAGTGATGCTTGATGCTGCAACGCTTAAAACAATGAAGATTTCTC2460.10346959634239183No Hit
CAACCAGGGCATCTGAGGATCGAGATCTAAAGCTTTGAGTCATGAGAATGAT2450.10304898822717885No Hit
GACACAGGGTCCCACAATTCTGCTTTAATTTCTGTCTATTCCAGAAAATCGT2440.10262838011196589No Hit
GGTTATGGGATAACCTCTTCCTCCTCTTTCACTTTTTCCTCTTCTTTTTTTC2400.10094594765111399No Hit
ATGATGGGGATCTCATTTGAGTTCACAGGCTGACAGAGACTTCCATTCTAAA2390.10052533953590101No Hit
TGCGGCGGGGTGGGCAGCCGGGGTCTAGGCACCACGGTGGCTGGCGGCCTGC2390.10052533953590101No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)