FastQCFastQC Report
Sat 31 Dec 2022
SRR5846826.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846826.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236972
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAAGGTCCATCTCGTATGC7740.32662086660027345No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAGGTCCATCTCGTATGCCGTCT7560.31902503249329034RNA PCR Primer, Index 16 (95% over 21bp)
GTTGTTGGGAAACAGATGGAAGAAGAAAAGCAGCAAACAGAAAGGGTTACAA3160.1333490876559256No Hit
AACGTAGGGAGACGCGCCGGAACCGGGACGCGATAAATATGCAGAGCGGAGG3040.1282851982512702No Hit
CGGCCAGGGATGTTTGAAAAATTTAGAAACTACTGGCAATTGTTAATAATGG2820.11900140100940194No Hit
AAGCATGGGTGTTAAAATAATCTCCTTCAATTTCTTTTCTCTTACTTCCAAG2760.11646945630707425No Hit
GAAACTGGGAACAGAAAAATTGCCACATGAGTCAAATAGCATAGGTTAGAAA2570.10845163141636988No Hit
TTCCGCGGGGGATGTAACATGCACCCAGGGCATTGCTTCAGAGACTATTTTT2540.10718565906520602No Hit
AGCCTGGGTGATAGAGCAAGATCCTGCCTTTAAAAAAAGAGAAAGGAAAATC2500.10549769593032089No Hit
GGTTATGGGATAACCTCTTCCTCCTCTTTCACTTTTTCCTCTTCTTTTTTTC2500.10549769593032089No Hit
TTTTGTGGGTCATTTCGTGCTTTCAAATTAATGATACTAGGCTTGTAGGATG2450.10338774201171447No Hit
TTGCTTGGGGAAATAGAAACAAAATGAACAAACCCATTTCAAATTAGGAGAA2440.10296575122799317No Hit
TTCCAGGGGGAGACATGGCGCTTGCCAGCGTGTTGGAGAGACCGCTACCGGT2430.1025437604442719No Hit
GAAGTTGGGAGAACATAGAGAAGGACCAAGGTTAATAAATGATTTTCATCCC2420.10212176966055062No Hit
TAAGAGGGAACATTGAAGCAGGCAATATCTGACTTTCATTCCTGAGTGAAAT2380.10043380652566547No Hit
AAATCAGGGGAAAAAAGCAGCAGCAAGATTAGACCTTAATTCCACTTGATCA2380.10043380652566547No Hit
TTGCGCGGGGGCCGGCGGGAGAGCTGGCTTGGGGCGCTGGCACCTCCTCTTA2380.10043380652566547No Hit
TAATATGGGGGCAGTTTGAATCGCGGTGCGACGAAGGAGTAGGTGGTGGGAT2370.1000118157419442No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)