FastQCFastQC Report
Sat 31 Dec 2022
SRR5846825.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846825.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236682
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAAGGTCCATCTCGTATGCCGTCT7220.3050506586897187RNA PCR Primer, Index 16 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAAGGTCCATCTCGTATGC7130.3012480881520352No Hit
GTTGTTGGGAAACAGATGGAAGAAGAAAAGCAGCAAACAGAAAGGGTTACAA3270.13816006286916624No Hit
AGCCTGGGTGATAGAGCAAGATCCTGCCTTTAAAAAAAGAGAAAGGAAAATC2770.11703467099314692No Hit
AACGTAGGGAGACGCGCCGGAACCGGGACGCGATAAATATGCAGAGCGGAGG2730.11534463964306538No Hit
GAAACTGGGAACAGAAAAATTGCCACATGAGTCAAATAGCATAGGTTAGAAA2690.11365460829298384No Hit
TTGCGCGGGGGCCGGCGGGAGAGCTGGCTTGGGGCGCTGGCACCTCCTCTTA2620.11069705343034113No Hit
GGTTATGGGATAACCTCTTCCTCCTCTTTCACTTTTTCCTCTTCTTTTTTTC2570.1085845142427392No Hit
TTGCTTGGGGAAATAGAAACAAAATGAACAAACCCATTTCAAATTAGGAGAA2520.10647197505513728No Hit
CATCTTGGGATTATAAAAGGGATTTTTTTCACCAGGATGAAAATGATAATGA2500.1056269593800965No Hit
GCGAAAGGGAATAAATAACCTTTTTCTATAAATATCTTGATTAGATTCAGTG2460.10393692803001495No Hit
GATTTGGGGAATGTTTGCCTACTGACTTCCTTGTCTTCTAGTGACTTCCTTC2440.10309191235497418No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)