FastQCFastQC Report
Sat 31 Dec 2022
SRR5846821.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846821.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162368
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAAGGTCCATCTCGTATGC9930.6115737091052423No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAGGTCCATCTCGTATGCCGTCT6330.38985514387071346RNA PCR Primer, Index 16 (95% over 21bp)
CTCAAACGGGAATGAGTGTCTTTCTTAATTATTTCTCATTTACTCTTTTTTA2440.1502759164367363No Hit
ATAGCTGGGGACCAAGTAGATTACTTTTTTAAATGACATGCCCAGTAGAAAA2390.14719649191959006No Hit
TATGCCGGGTACGAGGTCAAGAGATTGAGACCATCCTGGCCAACATGGTTGA2340.14411706740244384No Hit
TGTTGGGGGCTCCAGTTTGGAAATTCTTATGACATGTGAGTATCACAATTTT2230.1373423334647221No Hit
ACAAATGGGCTACTTCTTTTTCAGAACACTTCACAGTCATGATGTTATGGCT2210.13611056365786361No Hit
GTTTTTGGGCTTGGTCAGGCTGGTCTCGAACTCTCGACCTCAGGTGATCCGC2200.13549467875443436No Hit
GTTAGCGGTGGAGTTTATTTCTCAAAATTAAAATCTGGTCACATGAGCTTTA2200.13549467875443436No Hit
GTTTACGGGAGACAAACTAAAAAAGAATGTAATCAATTATCTGCGTGTTGGC2200.13549467875443436No Hit
CAATCGAGGGAGTCGAAGCTATTGGTCTTGGAAGATGAAAATGCAAATGTTG2200.13549467875443436No Hit
AGCTGTGGGAACCCTCATTTGGACATTAGCGAAGGGGCATTCTGATAACCAT2190.13487879385100512No Hit
ACTGTGGGGCAAACTGAAAATATTAATGTACAAACAAGATTATTAAAAACAG2170.1336470240441466No Hit
GTTCCGGGGGAAGAAACTGAATTTTATTCTTTGCATCCTTGTAATGTAAAAT2160.13303113914071737No Hit
ACAAATGGGGAAAAAAATTTTTTGAAAACTTTTTATTTCATTCTTTCCTGTA2140.13179936933385888No Hit
GAGGCAGGGAATTTCCTAGAAGGAGGATTATTAATTGGAGGATCTGACAACA2130.13118348443042965No Hit
CAACAAGGGTTAGCAAAAAGAATTGTGAAGAGGGTTTATATGAATGGATTAG2100.1293358297201419No Hit
TACGCGGGGCCGCCCAGCCTCACTTCCTTTGCCCTTCCTCCTGCCTTTCTCA2100.1293358297201419No Hit
TTGAGAGGGATGGTTTGGGTGAAAAGTTATTAAAAGTTCATTATAAATCTAG2100.1293358297201419No Hit
GCCCGAGGCTAAGTGGATAAGTGATACAGAATTCATCTACAGAGAACAGAAA2090.12871994481671267No Hit
TTTATCGGGTGCCCCTTTTTGGAGGTAAGCCCTTTTGTTCTTAAAAATTCTA2090.12871994481671267No Hit
CATAGGGGGGGGCCCTGCAGGATGCTGAGTGGCATCCCTGGCCTTCACCTAC2090.12871994481671267No Hit
AACTGTGGGAGAGTGCTGGGATTACAGGCCTGAGCCACCACGCCTGGTCAGA2090.12871994481671267No Hit
GGAATTGGGGCAGATTTCAGGTCATTATTGTTTTCTTTTAATGGTTTACATG2080.1281040599132834No Hit
AATGCCGGGGCCGGAAGAGGGGACGCCCTTGTGGCGGCTGCAGAAGCTGCCG2080.1281040599132834No Hit
TTGCCCGGGAGGGGCTGCTTGCCAGGGGAACCCACTTTGTGATTAGAGCCTT2050.12625640520299566No Hit
AGTAATGGGAAATCCAGAGCAGCAAAGATTTTTTGTTGTCTATTGTTTCTTT2020.12440875049270791No Hit
GATTCCGGGATCTAAGTAAGGGGAAGAACCAATGCAAATATGTTGATGATCA2020.12440875049270791No Hit
AACCGAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC2010.12379286558927868No Hit
ACAGCCGGGGTGGAGTTTGATCACACAATTCATAATGTTGCTGCTTTCCATT2010.12379286558927868No Hit
CAACCAGGGTACTCCTGCAGGTCAATTCCTGAAAGCAGATGAGCACTTTGCC2000.12317698068584942No Hit
TTGTCCGGGTTCTTACTACTGATGAGAACATTATCTGCATATGCCAAAAAAT1980.12194521087899093No Hit
ACAAGGGGCAAGCAAGTTTGCTGCTGTTGGATTTCATAAAACTTTGACAGAT1980.12194521087899093No Hit
GTGGTTGGGGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGC1970.12132932597556167No Hit
AAATTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1970.12132932597556167No Hit
AATGCGGGGGGTTGAATTATTATGTGTTTAAGGAATTCTAAGATAATAATTA1970.12132932597556167No Hit
GCTATCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1970.12132932597556167No Hit
TGCTGAGGGGAGAAGCAAACTTTGTAGGAAGCCACTCTTCTCCCCTAAACAA1960.12071344107213244No Hit
TTCAGAGGGAAATAAGTAATAGTAAATTATTTGTAAAGTGATTCATTATTAA1950.12009755616870318No Hit
TGTAGAGGGATATTATGACACTGACACTGCTATTTTGAAACCTGGAGGATGG1950.12009755616870318No Hit
GGGACCGGGATGTGAACAGACAGGAATTTCTTGTGCAATGTGGAGCAAATGG1940.11948167126527394No Hit
GCGCGAGGGGTGCTGGAGTCCTCAGCATCTGCTGGTCTTGATTCACTGATGG1940.11948167126527394No Hit
AGTCTGGGAATTAAGAAATAATTGTTAATTTATTACATATAGGTAATTTAAA1930.11886578636184471No Hit
CCTGTTGGGCATGTGGATGAACTCATTCAAACACAATGACTGCTTTATGAGA1910.11763401655498622No Hit
GCGAGGGCCATATAGCATGAGAAGCAATACACCATCTTGCATTGCATTTAAA1900.11701813165155696No Hit
TAGCTAGGATAAATATTCATGAGCCTGTTAATTGTTAGTTGTCTTCCTGTAG1900.11701813165155696No Hit
GAATCAGGGGGGCAAAAAAGAAAGATGGGAAACAATACAATTTAGAAAGGGA1880.11578636184469847No Hit
ACAGTAGGGGTATTGCCTGTTTGGTGCCCAATGGTTAAGTCATTGTCACTTA1880.11578636184469847No Hit
GCAAATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1870.11517047694126921No Hit
ATGCATGGGTATCTTCAGACCTAGGTTGTAAGACCAACTTTGGATTGGATCT1870.11517047694126921No Hit
GTATTGGGGAAACTAAAGCTATAACCATTAACCTAGAGAAAGATGGAACAGC1870.11517047694126921No Hit
CAACAAGGGGGGAATACAACTAACAAGGGAAATGAAGGACCTCTTCAAAGAG1860.11455459203783998No Hit
ATTGTCGGGCAACTTGGAATATGAAATACAAAGGTTTCCACACTAACTTAAT1860.11455459203783998No Hit
TTGTAAGGGACTTCAGCAAAATCTCAGGGTTCAAAATCAATGTGCAAAAATT1860.11455459203783998No Hit
CAACCGGGATTCTAATCCATGAGCATAAGATGAGTTTCCATTTGTTTGTATT1860.11455459203783998No Hit
CACGACGGGTGATCCGTACCTCGCTGGACCTGGAGTTAGACCTGCAGGCGAC1860.11455459203783998No Hit
TTATGCGGGAACTACAAAGTTTCTATACTTACTATAAGTGGATTTAGAAGCG1850.11393870713441072No Hit
GGACGAGGGGAGAATCACATTCTGATACGTTAATATCAGAAATCAGTGAGCA1850.11393870713441072No Hit
GCAAACGGGGGTGCAAAAATACCTTGCTGGTAAGCCATCAATGTTTTATTTA1840.11332282223098147No Hit
ACGTATGGGCAACACATCTAAGCATGAAAGAAATAAAAATTATAAGATAAAA1830.11270693732755223No Hit
GTTCAGGGGACATCAACTTCATTCTAAATGTTCATTCAAAATTATTTTTCAG1830.11270693732755223No Hit
TACTAAGGGAAGTCACATCCTACTAATAGTAACAATGGTATCAGAATAGCTT1830.11270693732755223No Hit
TAGCCCGGCCGATCTGTTCCTGGGGGTTTTTGGAGCTGCACCGAAGCATCTT1830.11270693732755223No Hit
TAGGCCGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGG1820.11209105242412298No Hit
TGTACAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1820.11209105242412298No Hit
CATTCAGGGCGTATTTTCTGATGTGGCTTTACATTTCAAAAGTGAACTTGAT1820.11209105242412298No Hit
CTCATGGGGATCTCAGACCCCAGTCTAATTTAGATACTGGCAAGCCCTCTGG1810.11147516752069374No Hit
TTATACGGGATATGCATACATATTTGATGTGCTTGAGAGATAGATGCTAAGG1810.11147516752069374No Hit
TTTTGTGGGAGTTGAGAAAGCTGTCTCCAAAAAAGGAGAAGTCTTTCCTTCC1800.11085928261726449No Hit
ACTCGCGGGGAGCGCAGGGCACGGTGGTCAGCGTGCAGCGGGACGGGACTGG1800.11085928261726449No Hit
CACCTGGGGACAAACACCTGCCAGGTGGCCCGGCTAGAGCCAGCCAGGGCCG1800.11085928261726449No Hit
GTCTTGGGGTAAATATAAATGGACTAAATTCTGCAATTAAAAGACACAGACT1800.11085928261726449No Hit
CGACTAGGGGATCCTGAGTGGAGGTTAGCTGAACGTTCAATGTACTGGAGCA1800.11085928261726449No Hit
GGACAAGGGGGCTTATGCAAATAATCATATTACCATAAGAATACTAACAAAT1790.11024339771383523No Hit
TCGTATGGGCACAGTTTTGGAGGCTGGCAAATGCAAGATCCAGGTGCTGGTA1790.11024339771383523No Hit
AATTTGGGATGAGAAAGAGAATCTCAGTGCAAAGAGGTCACGTATAGAACAG1780.109627512810406No Hit
GGTTAAGGGTCTAGGCATGGGACAGGAAGGAGAAATATGTACATATGTGTAT1780.109627512810406No Hit
TCTTAAGGGAAATTCAAACAACTTTCTTTTTTTAAAAAAATGCTATACCACA1780.109627512810406No Hit
CTTCCGGGGGATATTTGATAATGAAAAAAATCTTTGCTTGTTTATTTCTGAA1780.109627512810406No Hit
GCACATGTACCCCCTGAATCTAAAATGAAAATTGAAATTAAAAAATGTCTTC1770.10901162790697674No Hit
GCAGCTGGGGAAACTTGATTATTGCTGCCCTTTATAATTTCCTCTTCAATGA1770.10901162790697674No Hit
CACTCCGGGACCCCTAGTTTAAACACATGCTTGAAACATACACCTAATTTTA1770.10901162790697674No Hit
GTTAATGGGGGGGCACGCGGGCCATGGCTCCCTGGGCGGAGGCCGAGCACTC1770.10901162790697674No Hit
CGCACTGGGGGGATACTGCGAGTATGGCGGCGTCAAAGGTGAAGCAGGACAT1770.10901162790697674No Hit
CACTTCGGGGGATGAACTTTAAGGAAATTTTGTTGACTGAAAAAAAGCCAAT1770.10901162790697674No Hit
ACTAGAGGGCTGGGGCTGGGGGTTCTTAACCCTAGGCGCAGTTGTGCTTCTA1760.10839574300354748No Hit
AAGCTAGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA1760.10839574300354748No Hit
GGTACGGGCTGTTTAGTTTAAAAAGCATAAATATTTACTGAGCAGCCACTGT1760.10839574300354748No Hit
ATCGCAGGGGGAGGCGGCTACGGTGGCCAGAGCAGCATGAGTGGATACGACC1760.10839574300354748No Hit
TTCATTGGGAGCTAATATTTTTTTAAAGTTACATTAAGATTTTTTCTCTTTT1760.10839574300354748No Hit
GTATTTGGGAGTGACCGACTTCTCTCTCATGCTGTTTACCCCACACACAATT1750.10777985810011825No Hit
CTTAGCCGCGGGACTGGCTGGCACAGTACCTCCCTTTGTGTTTCTGCTTATT1750.10777985810011825No Hit
GACGTAGGGAGTCCAATGAGCATCTAGGAGAAAGCAGTAAGATGTTCTAAAT1740.107163973196689No Hit
GCTACGGGTGTAAGTCCCCCTGGTCCTGCAGGCCCAGTCCAGCCTCCAGAAG1740.107163973196689No Hit
AGTGAAGGGAGGGGAGGACAGCTTGGGCCCAGGAGTTTGAGGCTGCAGTGAG1740.107163973196689No Hit
GCTCAAGGGCATCTGCTTTCTAGATTTTGTACATCTCAGTCCATAAAGCAAA1730.10654808829325976No Hit
TGGGTTGGGGTAATATATTCCAAATACAAATCTACCAAATATATGTATTACA1730.10654808829325976No Hit
GATGCAGGGGACATTTGTTTAAGGATATGATAGTTTTATCCTGCATGTAATG1730.10654808829325976No Hit
CTTCCAGGGTGCCTTGCCTTGATACTGAAAGAAAAAAGAAAAGAAAAGAAAA1730.10654808829325976No Hit
ACATTAGGGATGTCTTTTTATTTTATTTTGAAGTCATTTATAGAATTTCTAA1720.1059322033898305No Hit
TGGTTGGGAGTACGGGGATGCGTCCCTCCGCCAGCTTCGGCAGCCTCGGAGT1720.1059322033898305No Hit
TGACCTGGGTCTTTCTCATTTCTCATATTGGTTCAGATTACTGCTAACATAA1720.1059322033898305No Hit
ATGGCTGTGGGATCTTTAGCCTACTTAAACAAAATACTTGTTAGCCAAGAAT1720.1059322033898305No Hit
CGATTTGGGTAGACGCCATGGCTGCATTGAATGATATACGGTATCAGAGACT1710.10531631848640126No Hit
AACAGTTGGGGATCTTATGGGGGGCTACTGGGAAATATTTGGATTTCAATGA1710.10531631848640126No Hit
TATCTAGGGATATTATTAAACTTTAAGAGTCAAAACAAATTTATGAACCATT1710.10531631848640126No Hit
AAAAATGGGGATTTTTTTCATATGCCTGTTGGCCATTTGTATGTCTTCTTTG1700.10470043358297201No Hit
AAAATGGGGAAGTTTATAACAAAAGGAACACTCACTTGCCTGGAATACCCAC1700.10470043358297201No Hit
TACAGTGGGCAATATGCCATTGATGAATATTATCGGATGAAGAAGGAGGAAG1700.10470043358297201No Hit
TTGAGCGGGGAGGTGGTTACATTCGTTGAAGGACACCAGCTGCGGAATTTGC1700.10470043358297201No Hit
TCCCTTGGGGACTCCACAAGAGGCTAGGAGCTGGGGGGAGAGAGGCAGTCCA1700.10470043358297201No Hit
CACCACAAAAATATGTTAATTACAAAGGAAAAGAGAATAATTTTACAGACAA1700.10470043358297201No Hit
CTCGGGCGGGGGAGAAGGAGGGCAGTGAAGATGAGCACTCGGGCAGCGAGAG1700.10470043358297201No Hit
AGCCTTGGGGGAAGCCAGTGACATAAAGATAATGAAGAAATAAAGAATTGTT1690.10408454867954277No Hit
ACTCTGGGGGCCTCCCTTGATGGTTCTTTATTCCCTTTTCATAGTATTGTCT1690.10408454867954277No Hit
CAGTAAGGGGTTGTCTCATGTTTGAAATATATAGCACTTTTTTTGTCTTTTT1690.10408454867954277No Hit
ACTCCAGGGGGTAGGAGGAAGATGGCGGTGGAGTCGCGCGTTACCCAGGAGG1690.10408454867954277No Hit
CTGAATGGGCACATATGAGGCTTAAATATAAGGAACATAACACTTGCATTCT1680.10346866377611352No Hit
GGGTGTGGGATTGCAAGAGGCGAGTTTTAAAATTCTGCAGATGGCCTTATTT1680.10346866377611352No Hit
TCCTTGGGGGCTTCTGTCTACGGCTTTGTTGATTTAAATTACTCGACACTAA1680.10346866377611352No Hit
AAGTCAGGGGAAAAGGAAGGTGTCTAGGAGTGACACTGACGTGTTTGGGTCC1670.10285277887268428No Hit
AGCGCAGGGTAGCCTACAGAACTTAAAGTGAGGGAACCATTTCCTCTCACAG1670.10285277887268428No Hit
AGTACAGGGCGATCTTTGTAAGGGTCTCTGGCAATACTGTAGGCCATCGGTG1670.10285277887268428No Hit
TTAGTGGGGGGAAATGAGCTTTAAGCCACATTCCATGAACCGAAGGGCCTCT1670.10285277887268428No Hit
ATGCAAGGGAGAATTTCACCTGCTGCTTTGCTAAATTCTCTGCCATCCAGCC1660.10223689396925502No Hit
CGATGTGGGTCCTAGGCAGATAACTACACAAGAACAAAACGCTATATAGAAA1660.10223689396925502No Hit
GTTTTTGGGACTAGTGGTATTGTCAGATGCCGTTAGTATCTGAGATGTACTT1660.10223689396925502No Hit
TCAGCAGGGGTGCAAATATAAGTAAACGTATAACTAATGAAGCTATTATCTC1660.10223689396925502No Hit
CTTGCTGGGGTACCCAGGGACACAGAAACTGCGGAAGGCCGCAGGGTCCTCT1660.10223689396925502No Hit
ACACCACGCCTGGCTATTTACATATTTTTAAACTAGAGCCCCTTAACATATT1660.10223689396925502No Hit
ACAGCTGGGTGTGTATATGATGTGTGCTTTTCCTCTGGCTTCTTTCAATATT1650.10162100906582579No Hit
GCGTGAGGGTTTTTATCAGAGACTGCAACACAAAGTCAGGTGCATGGCTTGG1650.10162100906582579No Hit
GCCTGTAGAGCCAAAGAAAAGGAATGTAACCTAATTAATGCCAAGAAAAAAT1650.10162100906582579No Hit
TTACTCGGGGGGGGCTTTACGTCCCTGTGAACTTAGAGTGAAAGCAGCAAGC1650.10162100906582579No Hit
TTGCCTGGGAATCCTATTTAAAAATGAGCAAATGATCTGAGCAGATATTTCT1650.10162100906582579No Hit
TTTCCAGGGTGCTGGGATTATAGGCGTGAATCACTGCTCCCAGCCCCTTTTG1650.10162100906582579No Hit
GTCGAGGGGAGATATATTTCTAGTATTAGATTGAAATAAAAACCCATTCTAT1650.10162100906582579No Hit
GGCTGGGGCTGCATGATGATCTGAACAACAGACTAGCTAGGACACTAAAAGA1650.10162100906582579No Hit
AGCAGTGGGACTGATCATATTGTCCAAGGTAGAAACTTCTCTTGAAATGAAT1650.10162100906582579No Hit
ACGTAGGGGTACAATATCAGTGTTGACTTTGAACTTCTTTAACGAGATCATG1650.10162100906582579No Hit
GCCTGGGGAGTTCACTGGTTGACCCTGATGACATCATGAAACACATAGGGGA1640.10100512416239653No Hit
CAATATGGGCAACAAAACCACTTCAAGGAAAAGGAACTGGCAGTATTTGTTG1640.10100512416239653No Hit
TAGCAGGGATCTGGAACATATTATCCAATCATGCTCCAATTCCAAGTGCAGA1640.10100512416239653No Hit
CTGTTTGGGCATTTGCTGTAAAGGAAATGAGAAGTCATTTACAGTATATTTC1640.10100512416239653No Hit
GTTTAAGGGGAGAGCTGGGCGGGTTAAGGAGGAGGATGGCGCCGTCCTGCAG1630.1003892392589673No Hit
CGTCTTGGGATCACCTCGTTTGTTCATCACAGCAGCCTTATGAGGTAGCTCG1630.1003892392589673No Hit
AACTATGGGAAACAAGTTATCAGTAGAACCAAGGGCTAATGGTCAATCTTGT1630.1003892392589673No Hit
AAATGGGATGTACAGCATTTCTGATCTTTACTTTGCAAGATTAGTGATACTA1630.1003892392589673No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)