FastQCFastQC Report
Sat 31 Dec 2022
SRR5846819.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846819.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160759
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAAGGTCCATCTCGTATGC8880.5523796490398671No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAGGTCCATCTCGTATGCCGTCT6150.38256022990936744RNA PCR Primer, Index 16 (95% over 21bp)
GTTCCGGGGGAAGAAACTGAATTTTATTCTTTGCATCCTTGTAATGTAAAAT2710.16857532082185134No Hit
ATTGTCGGGCAACTTGGAATATGAAATACAAAGGTTTCCACACTAACTTAAT2330.1449374529575327No Hit
GTTAGCGGTGGAGTTTATTTCTCAAAATTAAAATCTGGTCACATGAGCTTTA2280.14182720718591182No Hit
ACAAATGGGCTACTTCTTTTTCAGAACACTTCACAGTCATGATGTTATGGCT2270.14120515803158767No Hit
GTATTGGGGAAACTAAAGCTATAACCATTAACCTAGAGAAAGATGGAACAGC2230.13871696141429096No Hit
GGACAAGGGGGCTTATGCAAATAATCATATTACCATAAGAATACTAACAAAT2220.13809491225996678No Hit
AGCTGTGGGAACCCTCATTTGGACATTAGCGAAGGGGCATTCTGATAACCAT2220.13809491225996678No Hit
AACCGAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC2200.13685081395131843No Hit
CAACCGGGATTCTAATCCATGAGCATAAGATGAGTTTCCATTTGTTTGTATT2190.13622876479699428No Hit
CAACAAGGGTTAGCAAAAAGAATTGTGAAGAGGGTTTATATGAATGGATTAG2160.13436261733402172No Hit
ACAAATGGGGAAAAAAATTTTTTGAAAACTTTTTATTTCATTCTTTCCTGTA2110.13125237156240085No Hit
GCGCGAGGGGTGCTGGAGTCCTCAGCATCTGCTGGTCTTGATTCACTGATGG2090.13000827325375253No Hit
GCTCAAGGGCATCTGCTTTCTAGATTTTGTACATCTCAGTCCATAAAGCAAA2070.12876417494510414No Hit
GGACGAGGGGAGAATCACATTCTGATACGTTAATATCAGAAATCAGTGAGCA2070.12876417494510414No Hit
CAATCGAGGGAGTCGAAGCTATTGGTCTTGGAAGATGAAAATGCAAATGTTG2060.12814212579078No Hit
CTCAAACGGGAATGAGTGTCTTTCTTAATTATTTCTCATTTACTCTTTTTTA2050.12752007663645581No Hit
ACTGTGGGGCAAACTGAAAATATTAATGTACAAACAAGATTATTAAAAACAG2050.12752007663645581No Hit
ATAGCTGGGGACCAAGTAGATTACTTTTTTAAATGACATGCCCAGTAGAAAA2050.12752007663645581No Hit
AATGCCGGGGCCGGAAGAGGGGACGCCCTTGTGGCGGCTGCAGAAGCTGCCG2050.12752007663645581No Hit
GAGGCAGGGAATTTCCTAGAAGGAGGATTATTAATTGGAGGATCTGACAACA2040.12689802748213164No Hit
GTTAATGGGGGGGCACGCGGGCCATGGCTCCCTGGGCGGAGGCCGAGCACTC2030.12627597832780746No Hit
TCCCTTGGGGACTCCACAAGAGGCTAGGAGCTGGGGGGAGAGAGGCAGTCCA2030.12627597832780746No Hit
AACTGTGGGAGAGTGCTGGGATTACAGGCCTGAGCCACCACGCCTGGTCAGA2030.12627597832780746No Hit
GCCCGAGGCTAAGTGGATAAGTGATACAGAATTCATCTACAGAGAACAGAAA2010.12503188001915913No Hit
ACTAGAGGGCTGGGGCTGGGGGTTCTTAACCCTAGGCGCAGTTGTGCTTCTA2000.12440983086483494No Hit
TTGCCTGGGAATCCTATTTAAAAATGAGCAAATGATCTGAGCAGATATTTCT1990.12378778171051076No Hit
CATAGGGGGGGGCCCTGCAGGATGCTGAGTGGCATCCCTGGCCTTCACCTAC1990.12378778171051076No Hit
GGTACGGGCTGTTTAGTTTAAAAAGCATAAATATTTACTGAGCAGCCACTGT1980.12316573255618658No Hit
AGTCTGGGAATTAAGAAATAATTGTTAATTTATTACATATAGGTAATTTAAA1980.12316573255618658No Hit
TAGCCCGGCCGATCTGTTCCTGGGGGTTTTTGGAGCTGCACCGAAGCATCTT1980.12316573255618658No Hit
GATTCCGGGATCTAAGTAAGGGGAAGAACCAATGCAAATATGTTGATGATCA1980.12316573255618658No Hit
GTGGTTGGGGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGC1970.12254368340186242No Hit
GCAGCTGGGGAAACTTGATTATTGCTGCCCTTTATAATTTCCTCTTCAATGA1960.12192163424753824No Hit
TATGCCGGGTACGAGGTCAAGAGATTGAGACCATCCTGGCCAACATGGTTGA1950.12129958509321406No Hit
AGTAATGGGAAATCCAGAGCAGCAAAGATTTTTTGTTGTCTATTGTTTCTTT1950.12129958509321406No Hit
GGCTTTGGGTCGGCGCGGGGGGTGAATCTTCTAAGTGATTGACAAGGTGAAG1950.12129958509321406No Hit
TGTTGGGGGCTCCAGTTTGGAAATTCTTATGACATGTGAGTATCACAATTTT1940.12067753593888988No Hit
TATCTAGGGATATTATTAAACTTTAAGAGTCAAAACAAATTTATGAACCATT1940.12067753593888988No Hit
TGCTGAGGGGAGAAGCAAACTTTGTAGGAAGCCACTCTTCTCCCCTAAACAA1930.12005548678456572No Hit
AGCCTTGGGGGAAGCCAGTGACATAAAGATAATGAAGAAATAAAGAATTGTT1920.11943343763024154No Hit
GCACATGTACCCCCTGAATCTAAAATGAAAATTGAAATTAAAAAATGTCTTC1910.11881138847591736No Hit
ATGCAAGGGAGAATTTCACCTGCTGCTTTGCTAAATTCTCTGCCATCCAGCC1910.11881138847591736No Hit
TTTATCGGGTGCCCCTTTTTGGAGGTAAGCCCTTTTGTTCTTAAAAATTCTA1910.11881138847591736No Hit
ATGGCTGTGGGATCTTTAGCCTACTTAAACAAAATACTTGTTAGCCAAGAAT1910.11881138847591736No Hit
TTGAGAGGGATGGTTTGGGTGAAAAGTTATTAAAAGTTCATTATAAATCTAG1910.11881138847591736No Hit
TTACTCGGGGGGGGCTTTACGTCCCTGTGAACTTAGAGTGAAAGCAGCAAGC1900.11818933932159319No Hit
GGAATTGGGGCAGATTTCAGGTCATTATTGTTTTCTTTTAATGGTTTACATG1890.11756729016726901No Hit
GGTTAAGGGTCTAGGCATGGGACAGGAAGGAGAAATATGTACATATGTGTAT1890.11756729016726901No Hit
TTGTAAGGGACTTCAGCAAAATCTCAGGGTTCAAAATCAATGTGCAAAAATT1890.11756729016726901No Hit
CACGACGGGTGATCCGTACCTCGCTGGACCTGGAGTTAGACCTGCAGGCGAC1890.11756729016726901No Hit
ACAGCCGGGGTGGAGTTTGATCACACAATTCATAATGTTGCTGCTTTCCATT1880.11694524101294484No Hit
GTTCAGGGGACATCAACTTCATTCTAAATGTTCATTCAAAATTATTTTTCAG1870.11632319185862067No Hit
ACTCGCGGGGAGCGCAGGGCACGGTGGTCAGCGTGCAGCGGGACGGGACTGG1870.11632319185862067No Hit
TGTACAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1860.11570114270429649No Hit
ACAAGGGGCAAGCAAGTTTGCTGCTGTTGGATTTCATAAAACTTTGACAGAT1860.11570114270429649No Hit
GCGAGGGCCATATAGCATGAGAAGCAATACACCATCTTGCATTGCATTTAAA1860.11570114270429649No Hit
GCGTGAGGGTTTTTATCAGAGACTGCAACACAAAGTCAGGTGCATGGCTTGG1850.11507909354997231No Hit
TTACAAGGGCTTGGCATGTTTTCCAAACAATCCATTCACTTATTTAAACATT1850.11507909354997231No Hit
GTTTTTGGGATCTAGTAGGTCTGCACACATAACACACATCAGTCCACCCCCA1840.11445704439564815No Hit
TTGTCCGGGTTCTTACTACTGATGAGAACATTATCTGCATATGCCAAAAAAT1830.11383499524132397No Hit
AAAATGGGGAAGTTTATAACAAAAGGAACACTCACTTGCCTGGAATACCCAC1830.11383499524132397No Hit
ACACCACGCCTGGCTATTTACATATTTTTAAACTAGAGCCCCTTAACATATT1830.11383499524132397No Hit
TAATATGGGATCCGAAAGTCATTTCTTGGAACACAAGTGGAGTATGCCAAAT1830.11383499524132397No Hit
GCCTGGGGAGTTCACTGGTTGACCCTGATGACATCATGAAACACATAGGGGA1820.11321294608699979No Hit
ACGAAAGGGGCTGAGAACTTCATTGGCTAATTTCCAGAATGTTCAGAACCTT1820.11321294608699979No Hit
CAACAAGGGGGGAATACAACTAACAAGGGAAATGAAGGACCTCTTCAAAGAG1800.11196884777835145No Hit
ACAGCTGGGTGTGTATATGATGTGTGCTTTTCCTCTGGCTTCTTTCAATATT1800.11196884777835145No Hit
GTCGCAGGGATTCTTTAGCAGTGCTCAAGTGTAAATAAACTTGTTTACCAGG1800.11196884777835145No Hit
TGCACAGGGCATACCCAGCAGTATGAAGAAGTGAAGGATGAGTTGGGAATAC1800.11196884777835145No Hit
GTTTACGGGAGACAAACTAAAAAAGAATGTAATCAATTATCTGCGTGTTGGC1800.11196884777835145No Hit
TAGGCCGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGG1790.11134679862402727No Hit
TCAGCAGGGAAGACATTTAGTGCTGCCTCCTGGCAGTTATCTGTGTAATTGT1790.11134679862402727No Hit
TTTTGGGGGATAGCTGCCAGAGCAGAGCTGAGCCACCCATTGCTGGAGAGGA1790.11134679862402727No Hit
TATAGTGGGCCAGATCGTTAACTCTGAAAAAAAAGTCTTCCTTAAATTTATA1780.1107247494697031No Hit
CTTTATGCGGGAGATGTGTATATATATAAACATTTTACCCATTAGACATGTC1780.1107247494697031No Hit
TGACCTGGGTCTTTCTCATTTCTCATATTGGTTCAGATTACTGCTAACATAA1780.1107247494697031No Hit
TACAGTGGGCAATATGCCATTGATGAATATTATCGGATGAAGAAGGAGGAAG1780.1107247494697031No Hit
AAATTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1780.1107247494697031No Hit
GTTTTTGGGCTTGGTCAGGCTGGTCTCGAACTCTCGACCTCAGGTGATCCGC1770.11010270031537892No Hit
ATCGCAGGGGGAGGCGGCTACGGTGGCCAGAGCAGCATGAGTGGATACGACC1770.11010270031537892No Hit
GACGTAGGGAGTCCAATGAGCATCTAGGAGAAAGCAGTAAGATGTTCTAAAT1770.11010270031537892No Hit
AACAGTTGGGGATCTTATGGGGGGCTACTGGGAAATATTTGGATTTCAATGA1770.11010270031537892No Hit
TCGTATGGGCACAGTTTTGGAGGCTGGCAAATGCAAGATCCAGGTGCTGGTA1770.11010270031537892No Hit
GGGTGTGGGATTGCAAGAGGCGAGTTTTAAAATTCTGCAGATGGCCTTATTT1760.10948065116105474No Hit
ATAGCGGGGACAGTTTTCCATTATTTGTAACTTTGAATCTCTCTGTGGTAGC1760.10948065116105474No Hit
ACTTCAGGGGATTTAGCAAGTTCTAGAAGGTCTCCAAGACAATAATTACATT1760.10948065116105474No Hit
ACCAAAACAACATAGCAAACATACATATTTTGCATGCAAACCCTAAATATAT1760.10948065116105474No Hit
CAACCAGGGTACTCCTGCAGGTCAATTCCTGAAAGCAGATGAGCACTTTGCC1760.10948065116105474No Hit
AAAAATGGGGATTTTTTTCATATGCCTGTTGGCCATTTGTATGTCTTCTTTG1750.10885860200673057No Hit
TCAGCAGGGGTGCAAATATAAGTAAACGTATAACTAATGAAGCTATTATCTC1750.10885860200673057No Hit
TTGCCCGGGAGGGGCTGCTTGCCAGGGGAACCCACTTTGTGATTAGAGCCTT1750.10885860200673057No Hit
CAGTAAGGGGTTGTCTCATGTTTGAAATATATAGCACTTTTTTTGTCTTTTT1750.10885860200673057No Hit
TGGGTTGGGGTAATATATTCCAAATACAAATCTACCAAATATATGTATTACA1740.1082365528524064No Hit
TACATTGGGATCCACACCATCAAGGCTGTATCACCGAGTATCTGCTCATTCT1740.1082365528524064No Hit
TAGTCTGGGCAAGTATTTTTGTATATAACTGGTTAATTTTAATAAGAGATAC1740.1082365528524064No Hit
AAATGGGATGTACAGCATTTCTGATCTTTACTTTGCAAGATTAGTGATACTA1740.1082365528524064No Hit
GGTCAGACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1740.1082365528524064No Hit
ACTGTAGGGTGGAGGTTGCAGTGAGCTGTGATTGTGCCACTGCACTCCAGCC1730.10761450369808222No Hit
GGATTAGGGTGATCTAGTTTGACCTTTGAAATTTGCATGTGACCTCATCTAG1730.10761450369808222No Hit
CTTGCTGGGGTACCCAGGGACACAGAAACTGCGGAAGGCCGCAGGGTCCTCT1730.10761450369808222No Hit
CGACCTGGGATACGGTGAATATTGCGCAATTATAGATCTGGATTTTGAACCA1730.10761450369808222No Hit
TAGCTAGGATAAATATTCATGAGCCTGTTAATTGTTAGTTGTCTTCCTGTAG1730.10761450369808222No Hit
TACTAAGGGAAGTCACATCCTACTAATAGTAACAATGGTATCAGAATAGCTT1730.10761450369808222No Hit
TGTAGAGGGATATTATGACACTGACACTGCTATTTTGAAACCTGGAGGATGG1730.10761450369808222No Hit
CGACTAGGGGATCCTGAGTGGAGGTTAGCTGAACGTTCAATGTACTGGAGCA1730.10761450369808222No Hit
CTAGGCGGGGATAGGTCTTACTTATTTTAGGCTTGCTTTAAGTTCCTCTTTA1720.10699245454375804No Hit
GCTACGGGTGTAAGTCCCCCTGGTCCTGCAGGCCCAGTCCAGCCTCCAGAAG1720.10699245454375804No Hit
AACTAAGGGTCCTACTCAGGCACCCCCTTCACTCTGCCTACAGACTCAGTCT1720.10699245454375804No Hit
GCAAACGGGGGTGCAAAAATACCTTGCTGGTAAGCCATCAATGTTTTATTTA1720.10699245454375804No Hit
ATGCATGGGTATCTTCAGACCTAGGTTGTAAGACCAACTTTGGATTGGATCT1720.10699245454375804No Hit
TTTAATGGGCATTGGGGGAAAAAAAATAAGAAGATTCAACAGAATCAGCATT1720.10699245454375804No Hit
ACAGCTAGGGGATCTCAAAGCGGGAAGAACAGCAGTTGCATTTTATTCGTCA1710.10637040538943388No Hit
GTTTTTGGGACTAGTGGTATTGTCAGATGCCGTTAGTATCTGAGATGTACTT1710.10637040538943388No Hit
CTGAATGGGCACATATGAGGCTTAAATATAAGGAACATAACACTTGCATTCT1710.10637040538943388No Hit
GCAAATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1710.10637040538943388No Hit
TAGCAGGGATCTGGAACATATTATCCAATCATGCTCCAATTCCAAGTGCAGA1710.10637040538943388No Hit
TGACTTGGGTATCTCTCTGTGGAAATTATATATAATGCCTGGTATTTTACAC1710.10637040538943388No Hit
AATTTGGGATGAGAAAGAGAATCTCAGTGCAAAGAGGTCACGTATAGAACAG1700.1057483562351097No Hit
CACTCCGGGACCCCTAGTTTAAACACATGCTTGAAACATACACCTAATTTTA1690.10512630708078552No Hit
TTGAGCGGGGAGGTGGTTACATTCGTTGAAGGACACCAGCTGCGGAATTTGC1690.10512630708078552No Hit
CGGCCTGGGCCACAGAGTGAGACTCCATCTCAAAAAACAAAACAAAACAAAA1680.10450425792646134No Hit
GGCCATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1680.10450425792646134No Hit
CTCTCTTGGGGAGTGCTGCGCGGCGCTGTGTTTGCTTTTCTGGGGCGATACG1680.10450425792646134No Hit
CGATTTGGGTAGACGCCATGGCTGCATTGAATGATATACGGTATCAGAGACT1680.10450425792646134No Hit
GCTTTCGGGATCATAGTTCTTGGTCTAAAGTCTATCTTTTGGAAGATTTCAG1680.10450425792646134No Hit
TTCGACGGGATCATGCGTGCTTTCCATATTACTAAGTATTTCTCAAGAATAT1680.10450425792646134No Hit
TTTGTTGGGGGACTTTTGAAGATTTTTACCAACATCCTTAAATCAACTCCAG1680.10450425792646134No Hit
GTCTTGGGGTAAATATAAATGGACTAAATTCTGCAATTAAAAGACACAGACT1680.10450425792646134No Hit
GCCTGTAGAGCCAAAGAAAAGGAATGTAACCTAATTAATGCCAAGAAAAAAT1670.10388220877213719No Hit
TAGAATGGGATAGCACAATTCAAAACTTAAATTGTATAAAATGAACAAAAGA1670.10388220877213719No Hit
CACCTGGGGACAAACACCTGCCAGGTGGCCCGGCTAGAGCCAGCCAGGGCCG1670.10388220877213719No Hit
CTCGGGCGGGGGAGAAGGAGGGCAGTGAAGATGAGCACTCGGGCAGCGAGAG1670.10388220877213719No Hit
GTTTAAGGGGAGAGCTGGGCGGGTTAAGGAGGAGGATGGCGCCGTCCTGCAG1660.103260159617813No Hit
AGGCACTTAGTTTGCTACCACATTGTTCCCTTCATTGATTGAAACTGTAAAT1660.103260159617813No Hit
TATGCCGGGACGAGAATTCTTGACAGACTTCTGGATGGTTACGATAATCGGC1660.103260159617813No Hit
CCAAGCGGGACTGATGAACTAGATTAAAGCCTTGGGAGATTTATACTATAAA1650.10263811046348882No Hit
AACTATGGGAAACAAGTTATCAGTAGAACCAAGGGCTAATGGTCAATCTTGT1650.10263811046348882No Hit
GTCGAGGGGAGATATATTTCTAGTATTAGATTGAAATAAAAACCCATTCTAT1650.10263811046348882No Hit
ACTCCAGGGGGTAGGAGGAAGATGGCGGTGGAGTCGCGCGTTACCCAGGAGG1650.10263811046348882No Hit
CTCATGGGGATCTCAGACCCCAGTCTAATTTAGATACTGGCAAGCCCTCTGG1650.10263811046348882No Hit
TTCATTGGGAGCTAATATTTTTTTAAAGTTACATTAAGATTTTTTCTCTTTT1650.10263811046348882No Hit
GAATCAGGGGGGCAAAAAAGAAAGATGGGAAACAATACAATTTAGAAAGGGA1640.10201606130916464No Hit
ACAGTAGGGGTATTGCCTGTTTGGTGCCCAATGGTTAAGTCATTGTCACTTA1640.10201606130916464No Hit
TATCTAGGGCTGGCTGCCTCTTTCCAGAGTATGGAAGTCAGGAACTCTAACT1640.10201606130916464No Hit
GTGTCAGGGGGAATTGAGCCTTGTATTCTCAGCTTTGAGATCATTAAGCTCT1630.10139401215484047No Hit
AATTACGGGATACTTTAATATGAATTTCTATACTGAAAATCACACCTAACAT1630.10139401215484047No Hit
ATAAAGGGGTGTCTTAGAATTTCACTTTGAACCCAATGAATATTTTACAAAT1630.10139401215484047No Hit
AGCAGTGGGACTGATCATATTGTCCAAGGTAGAAACTTCTCTTGAAATGAAT1630.10139401215484047No Hit
CTGTTTGGGCATTTGCTGTAAAGGAAATGAGAAGTCATTTACAGTATATTTC1630.10139401215484047No Hit
TACGCGGGGCCGCCCAGCCTCACTTCCTTTGCCCTTCCTCCTGCCTTTCTCA1620.1007719630005163No Hit
AAATGAGGGTCTAAAGAAGTTTTCTAGAATAGAATTGCCTGTGATGATGCAA1620.1007719630005163No Hit
GGAATGGGAGGTTATAATTTTCACTAAGATGTAGATATTTCTCTTATTGTTT1620.1007719630005163No Hit
AACAACGGGACACCTCATCTACACACATATTTTTGTAATATAATCTTTAAAC1620.1007719630005163No Hit
CACTTCGGGGGATGAACTTTAAGGAAATTTTGTTGACTGAAAAAAAGCCAAT1620.1007719630005163No Hit
TCTTAAGGGAAATTCAAACAACTTTCTTTTTTTAAAAAAATGCTATACCACA1610.10014991384619212No Hit
CCTACTGGGGATCTTAGCATGGGACAGATTTTCTTTTGTCCTTGTCCATTAC1610.10014991384619212No Hit
TGCTCAGGGATTGCCAAGGTCTGATTTTTCACACAAAAAATTGCAACCCCCA1610.10014991384619212No Hit
AGATGGGGAAAAGCAGATGGGTTTTCTCTCAGGAAGGACTGCCCCATTGACC1610.10014991384619212No Hit
GCCGATGGGTGGAACATTTAATAAAATGAGGGGAAATGGATTTATAAACTTG1610.10014991384619212No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)