FastQCFastQC Report
Sat 31 Dec 2022
SRR5846797.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846797.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178675
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAAGGTCCATCTCGTATGCCGTCT9390.5255351895900378RNA PCR Primer, Index 16 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAAGGTCCATCTCGTATGC9220.5160207079893662No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG3570.1998041136141038No Hit
TTTCGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2980.16678326570589058No Hit
TTTCCAGGAATTTTTAAATTGGTATGAAACAAAATAAAAATTTGTAGACACT2460.13768014551560095No Hit
CTATTTGGGAATGCTGAGTGGTGACTTATTGGGGCCTTGCTCATGTTACTTT2440.13656079473905136No Hit
GCATTTGGGTGAAACAAACATATTGCTGTGCGGTGGACAGCCACTTCACAGA2380.13320274240940255No Hit
TCTAGGGGGGACTGCGCGCGCTCCAGCGCCCCAGAAGGCAGGGGGCAGCCCG2340.13096404085630334No Hit
CTAAACGGGGGGCGACTGAGCCGGGTGGATGGTACTGCTGCATCCGGGTGTC2300.12872533930320415No Hit
GCACATGCACCCCTGTATCTAAAAGTTGAAATTTAAAATAAAAGAATACTAT2290.12816566391492934No Hit
TGGCGCGGGGAGATACAAAAGAACAGAAAGGCTAAAACCAACATAAATTTAA2250.12592696236183015No Hit
TAAACGGGGATCTTTAAAATGTTCATTTTTAACCTGAATTTGCTTTGAATAT2250.12592696236183015No Hit
ATACAAGGGGAATTTGAGACATTGCGCCAGGTCTGCAATTCTGTTTTATCTC2170.12144955925563172No Hit
ACTGACGGGCAAATATAAAAACCAAAATAAGCTAGTCAAAGAAGACTGGAAT2160.12088988386735693No Hit
AAGGCTGGGCAATCAACAAGTGTTTATTTGTTACCAATTCTCTCTTTCTCTC2150.12033020847908213No Hit
ATTAATGGGGGGCCGACGGGTCGCCGCTGCGCCGGGCCGGGATGGCGGCCAC2130.11921085770253254No Hit
CATGCAGGGGGAAAGAGATAGCTGACAAATTGTTTTCCTATCTTAATCTGAA2110.11809150692598293No Hit
GATGATGGGACCTGGGATGACAGGCGCCTGCCACCACGCCCGGCTAATTTTT2090.11697215614943333No Hit
TAACTCGGGAACGAAAGATGCCATTATTTCATGGTAGGCTGCTGGAAGATGA2090.11697215614943333No Hit
AATCGGGGGACGCTGGGAAGGAGAAGTTTTCCCTTTTCTCACATTTTGGTAT2090.11697215614943333No Hit
CTCCACGGGCTGAGTTATTACTTATCTTTGATTTGATATAGTTCTGCCCCAA2080.11641248076115854No Hit
AGTCTAGGGACATCTGGCTCCAAATCTCAATAATGCCAATGTTGAGAAACCC2070.11585280537288371No Hit
ACGGCTGGGGCAGGCTTTTCTGAATACCAAATCTGCTTTTTGTAAAGCGTAA2060.11529312998460892No Hit
AGATGTGGGATAAATGAAAATGCTGAGTGTAATTCTTTAAAAGTTTTGTAAT2060.11529312998460892No Hit
ATCTGTGGGATTTGCAAGTAAAATAACCTAAGAAATTTGCCTTCAACCTGAA2060.11529312998460892No Hit
ATTAACGGGACTTTTTATTCGTTCCCACATTTTGTGGATTAAGAAACTGAGA2040.11417377920805932No Hit
TTGCATGGGTATTATGATACGTTCTGTTAGAAATCAGTTTTCTTTCTTGCCT2010.11249475304323492No Hit
ATGTTAGGGATGCCAAAAAATTTTAAGCAAATGAAAGCTACCAATTTAAAGT2010.11249475304323492No Hit
AACATCGGGGCACATAAACATGAAATGTGTTTTCCCCTGTGTACTAACACAT1990.11137540226668532No Hit
TGTATGGGGATTTTATATTCGTATAGTAGAATGGTCTATTTTTAATACCTGT1970.11025605149013573No Hit
ATATGCGGGGGGTAAAATATTTGAATAGACATTTCTCAAAAGAAGACATACA1960.10969637610186093No Hit
TTCTACGGGAAGATAACAGCCAACTAATTTTATTGACATTAATTTGCTATAT1960.10969637610186093No Hit
AGATTTGGGACATATTTCAACCCACGCTTGTAAAAGACATAAGAGTTTATAT1960.10969637610186093No Hit
GTATCAGGGCAGTAAAGCCCTGGTTGCAATTTTGGCTGTATATATTTTTTAG1950.10913670071358611No Hit
GATACTGGGGTGACCCTGATGATGGATGCCACCAATATGCCAGCTGTCAAAG1950.10913670071358611No Hit
TAATATGGGTAAATTAAAACTGAATTACCTTTTCTAATGTTTTTTTTTTTTT1950.10913670071358611No Hit
TAGACAGGGAGTGAAGGGAACCATATGTAGTTATAAAAAGTCACTGGTAAAA1950.10913670071358611No Hit
GCCCGAGGGAAGCATGGAACATGCAGGTGATTTGGGAAGTGTAGAAAGACCT1950.10913670071358611No Hit
CGGATTGGTATGTGGTGTCTTTACAATAATTTAAAATAATTCAGTAAATTGA1940.10857702532531131No Hit
GCTACAGGGCTACATGCGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGA1940.10857702532531131No Hit
AGCTTAGGGAAGTACAGAATATAAAATCATGACTTTGATGCAAATTAGATAT1940.10857702532531131No Hit
GCACACGGGGATCTTGCTGTGGGATGACCCCAATACGCAAAACTAACCCCCT1930.10801734993703652No Hit
AATCTAGGGGTTACTGCAAAGTCCTGTAAAATTTCTAAATTGCAAGTATTTT1930.10801734993703652No Hit
CAGTTTGAGAAACAGTGCATTTCTGAATGACACTGACCTGTAGGAAATGTTT1930.10801734993703652No Hit
TTATCTGGGGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATTG1920.10745767454876172No Hit
ACTTTAGGGATTTTCCAATAGGCCCCACAAAAATCATTGCTCTAGAGTAATT1920.10745767454876172No Hit
GTCGCCGGGGGGGTGGATTTGGATGTGATAATCAATGTATTCATAATTGGCA1920.10745767454876172No Hit
TAGTTTCGGGAGGAACTGTAAGCTTTTATCTTTTAACCAACTGAACAATACA1910.10689799916048692No Hit
CAACCGGGGTGCACACATTTTGAAGAGGAATTCTTAGAAACAATAAGAGCAT1900.10633832377221213No Hit
TGCTTAGGGGTTTGAATTGTTTTCAAAACACCTGTGTGTGTGTTTCTAAAAT1880.10521897299566252No Hit
GGTGAACGGGACAAAAACAATACTGGCAATAGTGAAAAGAAAACACCTTGTT1880.10521897299566252No Hit
TAGTTGGGGCATTTTGGTCTTCTGTTTAGTGTTGTATTTCAATTGTGGAGCC1880.10521897299566252No Hit
TCGTCCGGGAGGAGTAGGTTTTCAAATGTCCCTATTCCACTATTCGGAAAGT1880.10521897299566252No Hit
TCAGGGGGGTACATTTATGTAAAGACATAAGTTACTGACTTTTAATCTATTA1870.10465929760738772No Hit
AGGTTTGGGATTTGGAGGCCAGTGACTATGCTTCATCTATTTCTCTGAGAAA1870.10465929760738772No Hit
TTATCAGGGGGGCAAGGGCATCTGCATTTGATTTTTTATTATTTTATGTTCC1870.10465929760738772No Hit
CAACAGGGAATGAATTTTTTCAAACTCTTAGATTCAGTGAGTGAACAATACA1870.10465929760738772No Hit
CATTCAGGGTCTAAAACCTAATTGCAAATTGACCACAGACCTCTAACCTCCT1870.10465929760738772No Hit
GGTCCCTCCTCAATTTTCAGACATTTTGAAAGGATGACAGTTCTGTTTGTTA1850.10353994683083813No Hit
GAACCAGGGCGATCTCACGGAGGGTATCCCAATTCTTGAACGTTTGCTCAGG1850.10353994683083813No Hit
TACGCTGGGAAGCATTAGAATGTTACCTAATCTCTTCTGGGTTAAATGTGAT1850.10353994683083813No Hit
AAGTTAGGGAAGTTTTTTTCTCTTTGAAAGATAGAGATTAATACAACTACTT1850.10353994683083813No Hit
TCACCGGGTGAATGAGAAAGTGAGTGCTGTAAACACTGCAGTACTAGTGTGT1840.1029802714425633No Hit
TTCAAGGGGGATAGCAAACATAGAAATGATGTATATTGTTTTTTGTTATCTA1840.1029802714425633No Hit
GTAAATGGGCAGCCAGTGATTTGGAAGGTCTGTTTTCCTATAATTACTAAAA1840.1029802714425633No Hit
AAGTAGGGGCAGGCAGGGATTTTGATATGTGGTTAAGTTTATCTTTAGGTTT1830.1024205960542885No Hit
CGTACTGGGAGGACTGCAAGTCTAACGAAGTCTAGCCATGGCCCCTTTTACA1830.1024205960542885No Hit
GCCAGGGTTTAGGAGAACTTCATTCTTTCAAATTGGAATTCCAATTTCTGAA1830.1024205960542885No Hit
AGTCAAGGGGGGGAAAACTGGGTAAAAAGAACACAGGATCTCTGTATTATTT1830.1024205960542885No Hit
ACTGCTGGGATTACCAGCGTGAGCCACTGCGCCTGGCTGGCTTGTGCCTTTT1820.10186092066601371No Hit
GTCAAAGGGACGACCAGAGGAGCTGTCCCTGCGGTCACACAGGCTTAATCAC1820.10186092066601371No Hit
AACTGTGGGACGTTAATAGCCTTCACCAAGCTATTAGGAGCTCCTACACACA1820.10186092066601371No Hit
ACAGTTGGGATACCCATTAGGAAGCAATTCAAGATAGAGGGATGTGAACAAA1820.10186092066601371No Hit
AGCAAAGGGAGAGTCTGATCTAGGTGATCTCTAAGACAATTTTATGACAGAA1820.10186092066601371No Hit
TATTTCAGGGGGTAGTTTGTAGCGGACAACATGGCGGCCTTCATGCTGGGCT1820.10186092066601371No Hit
TAATCAGGGCGATCTAAAGCGGGGGTCTACTAAAAATATAAAAAATTAGCTG1820.10186092066601371No Hit
AGAATTGGGAGTTTCAGTCTTTCAAAGCCAGCCAGGCTACACACAGAAAATG1810.10130124527773891No Hit
AGGTCTGGGATTGAAAAAGCAAAGAATATATTTGATTTCCTACTAGTGATAA1810.10130124527773891No Hit
GATCATGGGCATATCCTACATGGTTAAAATGTAAGTTCTGCAGTTGAATGGA1810.10130124527773891No Hit
ATTCGCGGGTGCTCAGGACATTTGAGGTCAAATTGAAGACGGAAACCGGAAA1810.10130124527773891No Hit
GCGCTTGGGGCATGACTAACTAGCAACCCCATAAACCAATAAAGCATGAAAC1810.10130124527773891No Hit
TACGACGGGAGAAGTTGAAGAAGATGGCCAGTTAAAGTCCTTAACAATAAAT1800.10074156988946412No Hit
AATATAGGGGATCTCCTTTTATTTTTAGTCTGTTAATATTTAATGTAATATT1800.10074156988946412No Hit
ATCTCAGGGAGATCCTTCCCATACTGAACTCAGCCCTGGGTTGAACCCAAGA1800.10074156988946412No Hit
CTGTTGGGTATAAGGAAAGATTTCCGTATCTTCATAATATCTCCTGTTTCAT1800.10074156988946412No Hit
GCGACTGGGCTGTGGTGACAGAATCTTTGCCTAATTCCACCCAGCACAGCTG1800.10074156988946412No Hit
ATAGTTGGGCACTTGGGCTTTTTTTCTTCCCCCTCTTTCTTTAAAGAACTGC1800.10074156988946412No Hit
TGGCCAGGGTACGGAACCTGCTGTGAGCCTTCACTTGTACAGTCCACCTTTT1800.10074156988946412No Hit
ACTTGGGCAGACGGAACTGGAAAACCAGCTGGAGTACAATAAGAGGCGAGAA1790.10018189450118932No Hit
TTTTGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1790.10018189450118932No Hit
ATTGGTGGGTCATTAATGAATACTTCCTAATATCGTTGGCTCTACTAATATT1790.10018189450118932No Hit
TAAGCAGGGGAGTCTCCACCTTTGGACACAACACTTACTCACCATTTACTCT1790.10018189450118932No Hit
GCTGCAGGGGTATTTAAAAGAAAATTGAGAGAAAGGACTACAGAGCCCCGAA1790.10018189450118932No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)