FastQCFastQC Report
Sat 31 Dec 2022
SRR5846796.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846796.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179152
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAAGGTCCATCTCGTATGCCGTCT9250.5163213360721621RNA PCR Primer, Index 16 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAAGGTCCATCTCGTATGC9240.5157631508439761No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG3510.19592301509332855No Hit
TCTAGGGGGGACTGCGCGCGCTCCAGCGCCCCAGAAGGCAGGGGGCAGCCCG2460.13731356613378584No Hit
TTTCGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2450.13675538090559972No Hit
TGGCGCGGGGAGATACAAAAGAACAGAAAGGCTAAAACCAACATAAATTTAA2440.1361971956774136No Hit
GCTGCAGGGGTATTTAAAAGAAAATTGAGAGAAAGGACTACAGAGCCCCGAA2360.13173171385192461No Hit
TAGTTTCGGGAGGAACTGTAAGCTTTTATCTTTTAACCAACTGAACAATACA2320.12949897293918014No Hit
ATTAATGGGGGGCCGACGGGTCGCCGCTGCGCCGGGCCGGGATGGCGGCCAC2300.1283826024828079No Hit
CATGCAGGGGGAAAGAGATAGCTGACAAATTGTTTTCCTATCTTAATCTGAA2270.12670804679824954No Hit
GCACATGCACCCCTGTATCTAAAAGTTGAAATTTAAAATAAAAGAATACTAT2260.12614986157006342No Hit
AATCGGGGGACGCTGGGAAGGAGAAGTTTTCCCTTTTCTCACATTTTGGTAT2230.12447530588550505No Hit
AATCTAGGGGTTACTGCAAAGTCCTGTAAAATTTCTAAATTGCAAGTATTTT2230.12447530588550505No Hit
ATATGCGGGGGGTAAAATATTTGAATAGACATTTCTCAAAAGAAGACATACA2200.12280075020094669No Hit
GCTACAGGGCTACATGCGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGA2200.12280075020094669No Hit
GCATTTGGGTGAAACAAACATATTGCTGTGCGGTGGACAGCCACTTCACAGA2180.12168437974457444No Hit
TGGCAAGGGATAAACAAGGTCGCTGGTCATCCTCTGGCACAGAACGAACGTT2170.12112619451638831No Hit
TAAACGGGGATCTTTAAAATGTTCATTTTTAACCTGAATTTGCTTTGAATAT2170.12112619451638831No Hit
AGTCTAGGGACATCTGGCTCCAAATCTCAATAATGCCAATGTTGAGAAACCC2160.12056800928820219No Hit
AACGGTCCCTGCGCTGCCGTGTAAGTATTCCATTCTGTAGACTGACCATTTG2140.11945163883182995No Hit
GTTCAAGGGCAGCATTCTGTTCTGCACTGTGGGCTGGCTCTCTGCCCCAACC2120.11833526837545771No Hit
ACTGACGGGCAAATATAAAAACCAAAATAAGCTAGTCAAAGAAGACTGGAAT2110.11777708314727159No Hit
ATGTTAGGGATGCCAAAAAATTTTAAGCAAATGAAAGCTACCAATTTAAAGT2110.11777708314727159No Hit
TTATCAGGGGGGCAAGGGCATCTGCATTTGATTTTTTATTATTTTATGTTCC2100.11721889791908548No Hit
CTCCACGGGCTGAGTTATTACTTATCTTTGATTTGATATAGTTCTGCCCCAA2100.11721889791908548No Hit
TTTCCAGGAATTTTTAAATTGGTATGAAACAAAATAAAAATTTGTAGACACT2100.11721889791908548No Hit
CAGTTTGAGAAACAGTGCATTTCTGAATGACACTGACCTGTAGGAAATGTTT2100.11721889791908548No Hit
TGACTAGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC2090.11666071269089935No Hit
TAATATGGGTAAATTAAAACTGAATTACCTTTTCTAATGTTTTTTTTTTTTT2070.11554434223452711No Hit
CTATTTGGGAATGCTGAGTGGTGACTTATTGGGGCCTTGCTCATGTTACTTT2060.11498615700634097No Hit
ACTTGGGCAGACGGAACTGGAAAACCAGCTGGAGTACAATAAGAGGCGAGAA2060.11498615700634097No Hit
CAGCCTGGGTAACAGAGTGAGATTCCATCTCAAAATAAAATAAAATAAAATA2060.11498615700634097No Hit
ACTGCTGGGATTACCAGCGTGAGCCACTGCGCCTGGCTGGCTTGTGCCTTTT2050.11442797177815486No Hit
AGCAAAGGGAGAGTCTGATCTAGGTGATCTCTAAGACAATTTTATGACAGAA2050.11442797177815486No Hit
ATCTGTGGGATTTGCAAGTAAAATAACCTAAGAAATTTGCCTTCAACCTGAA2050.11442797177815486No Hit
CAATCAGGGTATTGTATAGTTTCAATAACTTTGGCAAAGTCACAAAGCAAGG2040.11386978654996875No Hit
TCAGGGGGGTACATTTATGTAAAGACATAAGTTACTGACTTTTAATCTATTA2030.11331160132178261No Hit
GCCCGAGGGAAGCATGGAACATGCAGGTGATTTGGGAAGTGTAGAAAGACCT2030.11331160132178261No Hit
CTAAACGGGGGGCGACTGAGCCGGGTGGATGGTACTGCTGCATCCGGGTGTC2020.1127534160935965No Hit
TTGTTCGGGGTTATTCACTAAGCTATAAACATTTTCCTGAAAAGATGTCATT2010.11219523086541039No Hit
TACGACGGGAGAAGTTGAAGAAGATGGCCAGTTAAAGTCCTTAACAATAAAT2000.11163704563722425No Hit
AACTGTGGGACGTTAATAGCCTTCACCAAGCTATTAGGAGCTCCTACACACA2000.11163704563722425No Hit
TGTGGATGGGCAGTCCAGCATTCATTAATTATACTGTATTTATCAGTAACAC2000.11163704563722425No Hit
TAACATGGGGTCCTATGAAAGTCATTCTGGTAGCAATGGTAATAGTACTATT1990.11107886040903814No Hit
TTCTACGGGAAGATAACAGCCAACTAATTTTATTGACATTAATTTGCTATAT1990.11107886040903814No Hit
ATTAACGGGACTTTTTATTCGTTCCCACATTTTGTGGATTAAGAAACTGAGA1980.11052067518085203No Hit
GTCGCCGGGGGGGTGGATTTGGATGTGATAATCAATGTATTCATAATTGGCA1980.11052067518085203No Hit
TAAGCAGGGGAGTCTCCACCTTTGGACACAACACTTACTCACCATTTACTCT1980.11052067518085203No Hit
AAGTAGGGGCAGGCAGGGATTTTGATATGTGGTTAAGTTTATCTTTAGGTTT1970.10996248995266589No Hit
TTGCATGGGTATTATGATACGTTCTGTTAGAAATCAGTTTTCTTTCTTGCCT1970.10996248995266589No Hit
CATTCAGGGTCTAAAACCTAATTGCAAATTGACCACAGACCTCTAACCTCCT1970.10996248995266589No Hit
TATTTCAGGGGGTAGTTTGTAGCGGACAACATGGCGGCCTTCATGCTGGGCT1960.10940430472447978No Hit
TGAAATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1960.10940430472447978No Hit
CGGATTGGTATGTGGTGTCTTTACAATAATTTAAAATAATTCAGTAAATTGA1950.10884611949629364No Hit
GCAGCCGGTACTAATAGAAGGACTGAGAGTCAGGTGAGCTAGGAAGGCAATG1950.10884611949629364No Hit
GCGCTTGGGGCATGACTAACTAGCAACCCCATAAACCAATAAAGCATGAAAC1940.10828793426810752No Hit
TCGTCCGGGAGGAGTAGGTTTTCAAATGTCCCTATTCCACTATTCGGAAAGT1940.10828793426810752No Hit
ATTCGAGGGAAGTGGGAGAGAGAAAATGACTCAGGACGCCATTGTAACGGTT1930.10772974903992141No Hit
GCACACGGGGATCTTGCTGTGGGATGACCCCAATACGCAAAACTAACCCCCT1930.10772974903992141No Hit
CAACAGGGAATGAATTTTTTCAAACTCTTAGATTCAGTGAGTGAACAATACA1930.10772974903992141No Hit
TAGACAGGGAGTGAAGGGAACCATATGTAGTTATAAAAAGTCACTGGTAAAA1930.10772974903992141No Hit
ATTACTGGGGTTCTACCAAGGCTGTGGAACTCTCCTACGAGTTCTGCTGATC1910.10661337858354916No Hit
TTCCAAGGGGTGAGATTATGGTTATGTGAAGAAAGAAGACGTTTCTGCTTGT1900.10605519335536305No Hit
AGCTTAGGGAAGTACAGAATATAAAATCATGACTTTGATGCAAATTAGATAT1900.10605519335536305No Hit
AGGTTTGGGATTTGGAGGCCAGTGACTATGCTTCATCTATTTCTCTGAGAAA1890.10549700812717691No Hit
CGTACTGGGAGGACTGCAAGTCTAACGAAGTCTAGCCATGGCCCCTTTTACA1880.1049388228989908No Hit
TACTATGGGGATACAGGATACAGGATAAAAGTGGATAATAATGCCCAATTTT1880.1049388228989908No Hit
ACGGCTGGGGCAGGCTTTTCTGAATACCAAATCTGCTTTTTGTAAAGCGTAA1860.10382245244261856No Hit
CGTACAGGGCAGCAGATATAAAGAGAGACTACATGATATTGAATTTTATAAG1860.10382245244261856No Hit
AAATGAGGGCTGAGAATTTGTTATATGCCAGATATTCTAGGCGACCTCACCT1850.10326426721443244No Hit
GATGATGGGACCTGGGATGACAGGCGCCTGCCACCACGCCCGGCTAATTTTT1850.10326426721443244No Hit
CTGTCAGGGCACTTACTAGAGAGAAAAGAGCTCCAGGCCTGGGGAACAGCAC1850.10326426721443244No Hit
GTCTACGGGCATCAGAGGTTTAATTTGAAGTTTATGTTCACACACTGAAAAC1850.10326426721443244No Hit
AACATCGGGGCACATAAACATGAAATGTGTTTTCCCCTGTGTACTAACACAT1850.10326426721443244No Hit
CAGCCTGGGCAACAGAGAGAGACTCTGTCTCAAAAACAACAACAACAACAAC1840.10270608198624631No Hit
AGGTCTGGGATTGAAAAAGCAAAGAATATATTTGATTTCCTACTAGTGATAA1840.10270608198624631No Hit
GACTTTGGGGATCTGACAGGAGGTGGAGCTCGGGCAGTAATGCCCGCTGCTC1840.10270608198624631No Hit
GCTCTGGGCACTTATTTCTTTATGAGCCATTCCCAAAGATTGACTTCTCTGT1830.1021478967580602No Hit
ATTTTGGGGGACCAGTGCACCGGTCTTCAGGGCTTCTTGGTTTTCCACAGCT1830.1021478967580602No Hit
ACTATAGGGAGAATTATAGGATGTAATGAACATAATATCAGAATAAGAAAGA1830.1021478967580602No Hit
ACTTTAGGGATTTTCCAATAGGCCCCACAAAAATCATTGCTCTAGAGTAATT1830.1021478967580602No Hit
AGATTTGGGACATATTTCAACCCACGCTTGTAAAAGACATAAGAGTTTATAT1830.1021478967580602No Hit
TAAAATGGGGAGGGGCGAGAAGGGGAAAAATGGGAAATAGTCTGATTTTAAT1830.1021478967580602No Hit
TGTATGGGGATTTTATATTCGTATAGTAGAATGGTCTATTTTTAATACCTGT1820.10158971152987407No Hit
GGCGATGGGCATTTTATGTCTCCTTCATTTATGCAAGTCATGCTTTCATTCT1820.10158971152987407No Hit
TTCAAGGGGGATAGCAAACATAGAAATGATGTATATTGTTTTTTGTTATCTA1820.10158971152987407No Hit
CAACCGGGGTGCACACATTTTGAAGAGGAATTCTTAGAAACAATAAGAGCAT1820.10158971152987407No Hit
GATACTGGGGTGACCCTGATGATGGATGCCACCAATATGCCAGCTGTCAAAG1820.10158971152987407No Hit
GCGACTGGGCTGTGGTGACAGAATCTTTGCCTAATTCCACCCAGCACAGCTG1820.10158971152987407No Hit
CAAACTGGGCTACTAGGGGACTGGTACTTGCGTTGATTCCATTTGTATTTAG1820.10158971152987407No Hit
GTCCACGGGACTAGGGAACAGCAGATGAGGCACTATATGCGCTTCCAAACTC1820.10158971152987407No Hit
ATGCCAGGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCACCATGTTGA1810.10103152630168795No Hit
TTATCTGGGGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATTG1800.10047334107350184No Hit
GCCAGGGTTTAGGAGAACTTCATTCTTTCAAATTGGAATTCCAATTTCTGAA1800.10047334107350184No Hit
CAACTAGGGATCAGTCACTGAGAAAAAGGCTTAATTTAATGAAAGCTATGTA1800.10047334107350184No Hit
TGGCCAGGGTACGGAACCTGCTGTGAGCCTTCACTTGTACAGTCCACCTTTT1800.10047334107350184No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)