FastQCFastQC Report
Sat 31 Dec 2022
SRR5846795.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846795.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176558
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAAAGGTCCATCTCGTATGC9490.5375004247895876No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAGGTCCATCTCGTATGCCGTCT8570.48539290204918495RNA PCR Primer, Index 16 (95% over 21bp)
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG3540.20050068532720128No Hit
TTTCGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC3000.1699158350230519No Hit
TAAACGGGGATCTTTAAAATGTTCATTTTTAACCTGAATTTGCTTTGAATAT2380.13479989578495452No Hit
TAGTTTCGGGAGGAACTGTAAGCTTTTATCTTTTAACCAACTGAACAATACA2300.13026880685100647No Hit
CTCCACGGGCTGAGTTATTACTTATCTTTGATTTGATATAGTTCTGCCCCAA2240.12687049015054544No Hit
ATATGCGGGGGGTAAAATATTTGAATAGACATTTCTCAAAAGAAGACATACA2220.12573771791705843No Hit
CATGCAGGGGGAAAGAGATAGCTGACAAATTGTTTTCCTATCTTAATCTGAA2220.12573771791705843No Hit
TCTAGGGGGGACTGCGCGCGCTCCAGCGCCCCAGAAGGCAGGGGGCAGCCCG2190.1240385595668279No Hit
GTCGCCGGGGGGGTGGATTTGGATGTGATAATCAATGTATTCATAATTGGCA2150.12177301509985387No Hit
GGTGAACGGGACAAAAACAATACTGGCAATAGTGAAAAGAAAACACCTTGTT2120.12007385674962336No Hit
TTCTACGGGAAGATAACAGCCAACTAATTTTATTGACATTAATTTGCTATAT2110.11950747063287984No Hit
TCTCATGGGAACCAAGTAGATTTGTGAACTTTATTTCTTTAGTAATTATTTT2100.11894108451613633No Hit
TTTTGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2100.11894108451613633No Hit
TTATCAGGGGGGCAAGGGCATCTGCATTTGATTTTTTATTATTTTATGTTCC2090.11837469839939282No Hit
CTATTTGGGAATGCTGAGTGGTGACTTATTGGGGCCTTGCTCATGTTACTTT2060.11667554004916232No Hit
GCACATGCACCCCTGTATCTAAAAGTTGAAATTTAAAATAAAAGAATACTAT2060.11667554004916232No Hit
AGAACGGGAATGAAGTGACTTAAAACAGTCACTTTACATATTAAAAATGAGG2060.11667554004916232No Hit
CAGTTTGAGAAACAGTGCATTTCTGAATGACACTGACCTGTAGGAAATGTTT2050.11610915393241882No Hit
GCATTTGGGTGAAACAAACATATTGCTGTGCGGTGGACAGCCACTTCACAGA2040.11554276781567531No Hit
ATTAACGGGACTTTTTATTCGTTCCCACATTTTGTGGATTAAGAAACTGAGA2040.11554276781567531No Hit
CTGTCAGGGCACTTACTAGAGAGAAAAGAGCTCCAGGCCTGGGGAACAGCAC2030.1149763816989318No Hit
AATCTAGGGGTTACTGCAAAGTCCTGTAAAATTTCTAAATTGCAAGTATTTT2030.1149763816989318No Hit
TGCTTAGGGGTTTGAATTGTTTTCAAAACACCTGTGTGTGTGTTTCTAAAAT2020.1144099955821883No Hit
TTTCCAGGAATTTTTAAATTGGTATGAAACAAAATAAAAATTTGTAGACACT2020.1144099955821883No Hit
ATGTTAGGGATGCCAAAAAATTTTAAGCAAATGAAAGCTACCAATTTAAAGT2020.1144099955821883No Hit
TCAGGGGGGTACATTTATGTAAAGACATAAGTTACTGACTTTTAATCTATTA2010.11384360946544478No Hit
CGTACTGGGAGGACTGCAAGTCTAACGAAGTCTAGCCATGGCCCCTTTTACA2010.11384360946544478No Hit
CATTCAGGGTCTAAAACCTAATTGCAAATTGACCACAGACCTCTAACCTCCT2010.11384360946544478No Hit
AAATGAGGGCTGAGAATTTGTTATATGCCAGATATTCTAGGCGACCTCACCT2000.11327722334870127No Hit
AATATAGGGGATCTCCTTTTATTTTTAGTCTGTTAATATTTAATGTAATATT2000.11327722334870127No Hit
ACTGACGGGCAAATATAAAAACCAAAATAAGCTAGTCAAAGAAGACTGGAAT2000.11327722334870127No Hit
AATCGGGGGACGCTGGGAAGGAGAAGTTTTCCCTTTTCTCACATTTTGGTAT2000.11327722334870127No Hit
TCACCGGGTGAATGAGAAAGTGAGTGCTGTAAACACTGCAGTACTAGTGTGT1980.11214445111521426No Hit
GCTGCAGGGGTATTTAAAAGAAAATTGAGAGAAAGGACTACAGAGCCCCGAA1970.11157806499847076No Hit
AGTCTAGGGACATCTGGCTCCAAATCTCAATAATGCCAATGTTGAGAAACCC1960.11101167888172725No Hit
CAACAGGGAATGAATTTTTTCAAACTCTTAGATTCAGTGAGTGAACAATACA1960.11101167888172725No Hit
TGGCGCGGGGAGATACAAAAGAACAGAAAGGCTAAAACCAACATAAATTTAA1950.11044529276498374No Hit
GCGCTTGGGGCATGACTAACTAGCAACCCCATAAACCAATAAAGCATGAAAC1950.11044529276498374No Hit
TACTATGGGGATACAGGATACAGGATAAAAGTGGATAATAATGCCCAATTTT1930.10931252053149675No Hit
ATTAATGGGGGGCCGACGGGTCGCCGCTGCGCCGGGCCGGGATGGCGGCCAC1930.10931252053149675No Hit
TACGACGGGAGAAGTTGAAGAAGATGGCCAGTTAAAGTCCTTAACAATAAAT1920.10874613441475321No Hit
TGTCAAGGGTTGATTAATATCTTTTTTCAGACTTAGTCAACATGTGATATTC1920.10874613441475321No Hit
TAATATGGGTAAATTAAAACTGAATTACCTTTTCTAATGTTTTTTTTTTTTT1920.10874613441475321No Hit
AGAATTGGGAGTTTCAGTCTTTCAAAGCCAGCCAGGCTACACACAGAAAATG1910.1081797482980097No Hit
CTCGCTGGGCATCTAATAAAACCAATAAAAGATCTCCCAGATAGCTAATAAA1890.10704697606452271No Hit
ACTGCTGGGATTACCAGCGTGAGCCACTGCGCCTGGCTGGCTTGTGCCTTTT1880.1064805899477792No Hit
TTATCTGGGGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATTG1880.1064805899477792No Hit
CGCTAGGGTGATTGAAGAGACTTCCGAACACTTGCTATCTCTTGCTGCTGCT1880.1064805899477792No Hit
ATCCAAGGGAATTGGAGCAAAGTAGAATGGAAGCAGGAAGGAAAAATAAATA1880.1064805899477792No Hit
TAGTTGGGGCATTTTGGTCTTCTGTTTAGTGTTGTATTTCAATTGTGGAGCC1880.1064805899477792No Hit
AACATCGGGGCACATAAACATGAAATGTGTTTTCCCCTGTGTACTAACACAT1880.1064805899477792No Hit
ACTTGGGCAGACGGAACTGGAAAACCAGCTGGAGTACAATAAGAGGCGAGAA1870.1059142038310357No Hit
AAGTTAGGGAAGTTTTTTTCTCTTTGAAAGATAGAGATTAATACAACTACTT1870.1059142038310357No Hit
GTTCAAGGGCAGCATTCTGTTCTGCACTGTGGGCTGGCTCTCTGCCCCAACC1860.10534781771429219No Hit
GATGATGGGACCTGGGATGACAGGCGCCTGCCACCACGCCCGGCTAATTTTT1850.10478143159754869No Hit
AAGTAGGGGCAGGCAGGGATTTTGATATGTGGTTAAGTTTATCTTTAGGTTT1850.10478143159754869No Hit
ATCTGTGGGATTTGCAAGTAAAATAACCTAAGAAATTTGCCTTCAACCTGAA1850.10478143159754869No Hit
ACTTTAGGGATTTTCCAATAGGCCCCACAAAAATCATTGCTCTAGAGTAATT1840.10421504548080518No Hit
AGGTCCAGATCAGTTGACAGTGGCAATTAAACTGTAAATAACTTGCCCTGGG1840.10421504548080518No Hit
TTGTTCGGGGTTATTCACTAAGCTATAAACATTTTCCTGAAAAGATGTCATT1830.10364865936406166No Hit
AGCAAAGGGAGAGTCTGATCTAGGTGATCTCTAAGACAATTTTATGACAGAA1830.10364865936406166No Hit
TGCTTAGGGGCCTCGTAGGGTAGAAACCAATATTTTTCAGGATGCTGTATTT1830.10364865936406166No Hit
TATTTCAGGGGGTAGTTTGTAGCGGACAACATGGCGGCCTTCATGCTGGGCT1830.10364865936406166No Hit
CAGCCTGGGCAACAGAGAGAGACTCTGTCTCAAAAACAACAACAACAACAAC1820.10308227324731815No Hit
TTACGGGGGAATATTTATAATGTATATTCAATTATAAGACCAGAGCAAATTT1820.10308227324731815No Hit
TATAATGGGATATAGTGTATTTTTCAAAATAGCTAGAAGAAAAGATTTGAAA1800.10194950101383114No Hit
GAACCAGGGCGATCTCACGGAGGGTATCCCAATTCTTGAACGTTTGCTCAGG1800.10194950101383114No Hit
ACTCGCGGGACTTTTCTCAGAGTTATATTTTTGGAATTTTGATGTCTTGGGA1800.10194950101383114No Hit
TCAGCCGGGCATTTGAATCCAGTAATCAGTATGAACATCATTAATGATGAGT1800.10194950101383114No Hit
ACGGCTGGGGCAGGCTTTTCTGAATACCAAATCTGCTTTTTGTAAAGCGTAA1790.10138311489708764No Hit
CAGCCTGGGTAACAGAGTGAGATTCCATCTCAAAATAAAATAAAATAAAATA1790.10138311489708764No Hit
TGCAATGGGGCTATGAAAGGCACGTGCCTGTTCCAATAATTACCAGTACATC1790.10138311489708764No Hit
TTGCCAGGGAGGGGTTTTGAGTCATCCAGTCCTGGGCACTGAGGTCTGTTAG1790.10138311489708764No Hit
TAGACAGGGAGTGAAGGGAACCATATGTAGTTATAAAAAGTCACTGGTAAAA1780.10081672878034413No Hit
AGCAATGGGATGGAAATGTAATAGAATCTCACACATTATTTTATAGAAATAG1780.10081672878034413No Hit
TAAGCAGGGGAGTCTCCACCTTTGGACACAACACTTACTCACCATTTACTCT1780.10081672878034413No Hit
TTCTAGGGGGTACAAGGTTACATGGCTAGGAAAATATCAGACTGGGAATTTA1780.10081672878034413No Hit
GGTCCCTCCTCAATTTTCAGACATTTTGAAAGGATGACAGTTCTGTTTGTTA1770.10025034266360064No Hit
AACGGTCCCTGCGCTGCCGTGTAAGTATTCCATTCTGTAGACTGACCATTTG1770.10025034266360064No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)