FastQCFastQC Report
Sat 31 Dec 2022
SRR5846776.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846776.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126780
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGCCGTCT5350.4219908502918442TruSeq Adapter, Index 7 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGC5110.4030604196245464No Hit
TAACTAGGGAAAGTGACGGTCAGCAGGGGGTTCTGAGGCCCCACAGCGCTGG1880.1482883735604985No Hit
ATATGCGGGCATGCCGGGAGTGATCAGTCGGACATTTTAATTTGAACCACGT1860.14671083767155704No Hit
TGTAATGGGGGCTTTTGACAAGATGGCGGCAGGAGGCAGTGGCGTTGGTGGG1780.14040069411579115No Hit
TTGAGCGGGGGGCCGCCGGAGCGCTTTGGAAGGCGCACGGGGCGAAGATGGC1760.13882315822684965No Hit
AATCTCGGGATGGATCAGTGGTTTCTAACGTTGGTTTTGTGAAATGTTATGA1740.13724562233790819No Hit
GCGGTTGGGCAGTAATGATATTGTGAGTTAGGATAATAACTTTTTTTTTTTT1740.13724562233790819No Hit
TAACGAGGGAATACCCAACTGTCAATATCAGACAGATCGAGACAGAAAATTA1740.13724562233790819No Hit
TTAGCAGGGGTACTGCTAGAACTAATAAATAAATTCAGTATGATTAACAGGA1730.13645685439343747No Hit
GATATAGGGCAAAGGTATTGAATCAATAAATGAGAAACAAAGGCTTCTCTGG1720.13566808644896672No Hit
TGGTTGGGGGAAGAGAGGTTTGAGAAAAGCACTAAGGAATTGATGGCAATAA1720.13566808644896672No Hit
TTGCCTGGGTGCCAGTTTGTATTATAATTGTCTGAACTCATTGACCTACTAT1710.134879318504496No Hit
TATCTTGGGGAAATAACCCCAGAGGAGTGAGGGAAAATAACGTGTAGCCAGT1710.134879318504496No Hit
ATACCCGGGCAACAGAGTGAGAACCCGACTCATTTAAAAAAGGGGCAAAGGC1710.134879318504496No Hit
ACAGTCGGGATAGTTTGTGAACCCTTGTTCCAGTTGATTTTTTTCCTAGAAA1700.13409055056002525No Hit
TCACATGGGCTGGTCCTGGGTAAATAAAAACATGTTGCTTTGTATACATTTT1690.1333017826155545No Hit
ATGTGAGGGGGTATTAAAAGTGAGTTGAGGACCTAGTGCTATTCATTCAAAA1650.13014671083767154No Hit
CACACGGGACCAAAGGAATTTGCATCCAGCAGCAGCACTTAGACCTGCCAGC1640.12935794289320082No Hit
TTCGCAGGGGAGGGCGGCTGCTGCTGCTGGGACGTGCGGCTGGACCAGCCCC1590.12541410317084714No Hit
GACAATGGATCTCACGTGAAGTGAGCTTCGTTTGTGCTCATTAAAAGTGAGC1590.12541410317084714No Hit
TGAACTGGGATACTAAAATCATAGTTTGAATTGCTGACATATTAATTGTGGA1560.12304779933743494No Hit
GTCAAAGGGGAGGACACGGGTCGCTGGCGGGTTAGGTGGCTGCGTGGCCGCC1550.12225903139296418No Hit
GCAACAGGGCACCTTGGTGGTCATTCTTTCTTTGTCTTGAAGAATAGTGCCC1550.12225903139296418No Hit
GGAATTGGGGTGTAGAAGGGACACTTGCCAAGCACATCATGTCTGAACCTGA1540.12147026344849345No Hit
CTGCATGGGCCAGCCTGGGCAACAGAGTAAGACTCTGTCTCAAAAATGAATG1540.12147026344849345No Hit
GCTAATGGGGGATGAACATTCCCTCAGGGATTAGGGATAAGAGAAGTTAGCT1540.12147026344849345No Hit
TCACACGGGGAACCATCAGTCTGCAGTAATCCAGTCAATTAAAAATGGATTT1530.12068149550402271No Hit
GACGATGGGCATCCTTGTCTCTACCAAAAAATTTAAAAATTGGCCGGGCGTG1530.12068149550402271No Hit
CATGCGGTATGTCATGATATCATAAAAGGGACACTGGGAAATCATTTGATTC1520.11989272755955198No Hit
CATTGTGGGCTACAATTCTGAATTTGCTTTTCTTGCCTCTCTTTAGTCACCT1520.11989272755955198No Hit
AATACTGGGAGGTTTTTTTGTCTTTCCTTGCTTGTGTTTTTGGTACTATAGC1520.11989272755955198No Hit
AAACAAGGGCTCCATTTCTTTATGCCTGGCTTTGCCCCTCTCACCAGCCGTG1510.11910395961508125No Hit
GTACATGGGATTCATTATCTCTGTAGGAACTTTTAGTTCCATTATATGAACA1490.11752642372613976No Hit
ATTCTAGGGAGCTTTTTTTATTGAGTATAGTTTCTTTTAAAACATGGAATGC1490.11752642372613976No Hit
ATAAGGGGACCAACGATGTAAAAGCATTCCTATTTCTCCACAGCCTCGCTAG1490.11752642372613976No Hit
TCGACTGGGGCTCCTGAATGGCTACTGGGTAAATAATGAAATTAAGGCAGAA1460.11516011989272756No Hit
TATGCTGGGCTCACATCTGCAAAGGGATTTTGCAGATGTGATCAAAGTAAGG1460.11516011989272756No Hit
AGAATAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1460.11516011989272756No Hit
TCTTAGGGGAACACAGGGACAATACCATAGTCATCAAACCCCTTGAACACAG1460.11516011989272756No Hit
TTGCTAGGGGGCTGCCCGAGGGTTCCTCGCAGGCTGGAGCTGACGGCGCTGG1460.11516011989272756No Hit
AACTCAGGGCTCACTGCATCACATAGAATATTATAAAGTTATGGACTCAAAG1450.11437135194825683No Hit
ATTTTTGGGGCTTCTGAAATTCTACTCAGAATCGGAGCCGTTTGCATTCCAA1440.1135825840037861No Hit
TTCATAGGGTGCTTAAAGAAAGGCAGGTGCATTGCTCTGAAGTGCATATAGC1440.1135825840037861No Hit
AAAGTAGGGAATTAGCATATAGAAAAATATTACTTTAAGATGACTTGTTTCC1440.1135825840037861No Hit
CAATGAGGGATACTTTTTCTTAGTTTCTTGCCCTCTTCCCTTCTGATCAATA1430.11279381605931535No Hit
TCAATTGGGCTGTGGGCATGGGGTAGAGGGGACAGAATGTAGGTAACAGAAA1430.11279381605931535No Hit
TGATGCGGGAATGTGTTGAAACTAGCAGAAGCAGGGGCCTGTGACACATGAC1430.11279381605931535No Hit
TGACTTGGGTACCTTCTGTGTTGGTTTTGCTGGGAGCTGCAGACCAGAGCTG1430.11279381605931535No Hit
CAACGTGGGAGAGTAAAAGGGGAAATGCCAAGATTTTGGGGTTTTTTTGTTT1430.11279381605931535No Hit
GCACAAGGGGACATAGAATGGCTGAATGCATAAAAAGCAAGACTCATTTATC1430.11279381605931535No Hit
CTTTTGGGGCAATGAGACAGAAAATTAACAAGGATATTCAGGACTTGAACTC1430.11279381605931535No Hit
GTGATGGGGATTACTTGAGGACTGTGTCTGCTCCGTGAGAGCTGCGTGGGCA1420.11200504811484462No Hit
CGGTCCGGGGGCACTGGCCTTCAACCAAGCCAAGCGCCTTGGCTTCTGTCCT1420.11200504811484462No Hit
GCTTTTGGGCAGACACCCAAGGAGAGAGACTGCGAGGAAAGAAACAGAGTCA1410.11121628017037388No Hit
TCTCAGGGGAGAAGGAAGAAGAAGAGGGGGAGGAGAAAGAGATAGAAGAGTA1410.11121628017037388No Hit
GCAGCTGGGTACTAGTATTAGTTTTATTAATGCCCTATAAACCCAGCAAGCA1410.11121628017037388No Hit
AAAACTGGGAAGTCTGATAACACTGTGATTACATATGTATCAATATAGTATG1410.11121628017037388No Hit
AGGACTGGGACTTCCCTCTGGCCACATATGCCCCTGTCATCTCTGCTGAGAA1410.11121628017037388No Hit
GCTAACGGGATACTTTGCTTGGTCCTATAGGCTCTCAGGTTTTTTTGATATC1400.11042751222590313No Hit
CAAGCAGGGGGTGCTCGTTTGGTGCGGACCTGGTACCTCTTTTGTGAAGCGG1400.11042751222590313No Hit
ATACAGGGGCGATCTATGAGCGGGATGCTAACAGGATCTACAGGATGATCAA1400.11042751222590313No Hit
ACAAACGGGGGAAAGGATTTGATGAAATGTTCAAGTCAAATGCAGAATAAAG1400.11042751222590313No Hit
GATTATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1390.10963874428143239No Hit
TAAAAGGGGGTTAATAACTGATTAGGTTATCATGGTGAGTAGCCCTGAGATA1390.10963874428143239No Hit
GAAAATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1390.10963874428143239No Hit
TGGTTCGGGTAACTGAGGAAGGAACAGAGCTGTCACAACGACTTGTGAGGAG1390.10963874428143239No Hit
CGCCCAGGTGAATCTCAATCCTTCCCCCTGAATAGCACGTTTCAACTAGGAA1380.10884997633696165No Hit
GCTGCTGGGATCCGAGGTGCTTGGGAGTTTTGAATGTTAAGAATTGACCATC1380.10884997633696165No Hit
GAATACGGGGGATAGTCAATTCAATTTTATTAGTTTTAGAGTTATATGAATT1370.10806120839249092No Hit
GGCGCAGGGGATTGGCTGGTTGCCAAGAAATATATATTTGTCACTAGGGCAT1370.10806120839249092No Hit
TTAGCTGGGTGCACTTATTTTCCAGAAATAAAAATATGAAGAGCTCACGCAG1370.10806120839249092No Hit
TACCTGGGGGAAGCACAAGCAGAGCGGCGGGAGCGTGGGTGCGCTGGAGGAG1370.10806120839249092No Hit
CTTGCTGGGAACTCAGGGCTTCCATTTCCTCAACTGTAAAATTGGGAAAATA1370.10806120839249092No Hit
GTTGCCGGGCAAAATAAAACGTGATGAACGCACCTTAATAAATCCAGATATT1370.10806120839249092No Hit
CACAAGGGCATTATATTGAGTGAAAACTCCAATCTTGAAAGGTCACATCTGG1360.10727244044802019No Hit
CAGTGAAGGGCTGACCTACAGACTCCATCAGCATCCCAAGTGATTCTCACAC1360.10727244044802019No Hit
CCAAAAGGGGATGCCATGGTTTTACATCTTTTAATTTAAACCTTATACACCA1360.10727244044802019No Hit
CCATGAGGGAACTGATTCCATGCACTAGTACATGTAGGCTTCTCCCTTGTGC1360.10727244044802019No Hit
TTGCAGGGTAATTATGATTGGTCTGTGAGGCTAGAACATCCCGCAGAGGAAA1360.10727244044802019No Hit
CAATTAGGGTGCCTTATGGTAAAATTTACTTAAAACGTTAAACTAAATGAGA1360.10727244044802019No Hit
CTATTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1350.10648367250354945No Hit
CTTCAAGGGACAGCTTGATGAAGATTTACAAAGTAAACATGCCAGTTGAAAC1350.10648367250354945No Hit
AGAGTAGGGGGGGCTGCTGAACCTTGGGAAGAGTGTGAGTGTGAACGTGTGT1350.10648367250354945No Hit
TAAGTTGGGGAGAGGGGATGTAGGCCCATGGGCACCTGGCCTCCAGGAGTCG1340.10569490455907872No Hit
CGTACTGGGACACAATAAAAAACCAAAACCTGGAAAAGAAATACTTAAAAGA1340.10569490455907872No Hit
TACCTGGGGCATCAGTCCACCCCCATTGTGAATGTTGTCCTGTGTCATCTGT1340.10569490455907872No Hit
GATTTAGGGATCTGTCTTGTTTTTGTTTTTAAACAACCTGTTAAAAATGTAA1340.10569490455907872No Hit
GGCTGTGGACTTTCCAGGGACGCCTCCTGCAGAAGAACAACAAGGACCGCTT1340.10569490455907872No Hit
CGACCCGGGAATTCACTACATTAATAAACTGAAAAAAATCACATGATTATCT1330.10490613661460799No Hit
CGCTTTGGGGAAAGATGTTCATGGTTAAATGGCTTATCACTTCCCAGACTTT1330.10490613661460799No Hit
GTATTAGGGAGTGGAGATTTGGTTTATGTTACCATTAAGTTCCTCTAAAAAG1330.10490613661460799No Hit
TTATCGGGGAGGTGGAATTGGTAATGCACAAACTGTGAAAGTACTTTTCAAT1330.10490613661460799No Hit
TAGACAGGGCACCGAAGCCTTAAATCCTTCTGTCTTCCTGACTGAATGAAAC1330.10490613661460799No Hit
CTACTTGGGGTGGCCTGAGAGAGTACTTGATATAATTTAGATTTTCTTAAAT1330.10490613661460799No Hit
ATCAAAGGGATTTCATTTGATATTTAGCTTCTGAAAGCCCTTTGTTATAAAG1320.10411736867013725No Hit
CTGCAAGGGTTCCAGGTCAGAAGAAATTGTTTCTTAGACCTCTCAGACTAAC1320.10411736867013725No Hit
TAAAATGGGTCTGTATTTGAAATGTTGAGTACGGTTTCTGGTAAGTCACATT1320.10411736867013725No Hit
GCTTCAGGGCATGGCCTGCAGCCTGTTTCAATGCATCTGTCCTTAACCATCC1310.1033286007256665No Hit
GGTATAGGGGTGCTGCCCCGGCGGACTTTGAGCCTGCAGCCTGGCGGTGGGG1310.1033286007256665No Hit
CCTGGTGGTGTTATTAGCCAGAACTCTCTGAAATGTAAATAATTATACAATG1300.10253983278119577No Hit
TGTCTGTGGGTAGAATAGTATACCCAGTGAAAATATCTTTCAAACAGGAAGG1300.10253983278119577No Hit
TAGCAGGGGCGGCTTGAGAAGACTGTCAGGAAACGAGTATGTGTTTACTAAG1300.10253983278119577No Hit
TATCGGGGGTCTTCGTCTTAGTCCAGCAAGCACCGCCAATCCTTTTCTTCCC1300.10253983278119577No Hit
TCTTATGGGATTCCACAAGGATAAGTGCTTCCTGCTGGGACAAAGTCCTCAT1290.10175106483672504No Hit
CACCGTGGGATACTTCAGTGAGAGCCATTATAAGTGAAATCTAAAAATTGAT1290.10175106483672504No Hit
AATACAGGGATCGACTCGGACACAGAGGACAGCGGCAGCGACGAGAACCTGG1290.10175106483672504No Hit
TACGCCGGGCTATATCTGAAATTGTATTATCTATTTTTATTTCACCTTTCTT1290.10175106483672504No Hit
CGAGTAGGGAGGCCATGCAAATGAAAATTTTTCCTTCGTCTTACAGATAACT1290.10175106483672504No Hit
ACAATAGGGACCGTTTGTCTTGAAGACCCAGTGACACAGGAGAAAGCCAAAA1290.10175106483672504No Hit
GACAAAGGGGTCCAAAAAAAGAGATTTTCATGTCAATTACTGTGTTTCTCCT1280.1009622968922543No Hit
GCATTTGGGATTTGATTAAGATAGGTTCTTTCTGTATATGAAACGCTCCCCG1280.1009622968922543No Hit
TTTACAGGGGGGAAGCATTTTTACCTTATTGTAGGGCAGTTTTGTTTTTCAA1280.1009622968922543No Hit
CAGCCGGCGATCGGAGTGGGCCTTTTCTTTCTTTTTGTTCATTCTTTACCTT1270.10017352894778357No Hit
ATGTATGGGAATTTTTTATTGTGGTAACGGACAGGAATAATAACTACATTTT1270.10017352894778357No Hit
CTCCAGGGAAAGAACAACATTAATGGAGATGCACAATGATTATTCAAACAAG1270.10017352894778357No Hit
TCAAGCGGGCGATCTGTTTGCGGGTCACACTGCCAAGCAACCCACTAATGCA1270.10017352894778357No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)