FastQCFastQC Report
Sat 31 Dec 2022
SRR5846775.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846775.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126661
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGCCGTCT5050.39870204719684826TruSeq Adapter, Index 7 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGC5040.3979125381924981No Hit
TAACTAGGGAAAGTGACGGTCAGCAGGGGGTTCTGAGGCCCCACAGCGCTGG2080.16421787290484047No Hit
ATATGCGGGCATGCCGGGAGTGATCAGTCGGACATTTTAATTTGAACCACGT2030.1602703278830895No Hit
TGGTTGGGGGAAGAGAGGTTTGAGAAAAGCACTAAGGAATTGATGGCAATAA2000.15790180087003894No Hit
TTAGCAGGGGTACTGCTAGAACTAATAAATAAATTCAGTATGATTAACAGGA1900.150006710826537No Hit
TGTAATGGGGGCTTTTGACAAGATGGCGGCAGGAGGCAGTGGCGTTGGTGGG1900.150006710826537No Hit
ATACCCGGGCAACAGAGTGAGAACCCGACTCATTTAAAAAAGGGGCAAAGGC1890.14921720182218678No Hit
AATCTCGGGATGGATCAGTGGTTTCTAACGTTGGTTTTGTGAAATGTTATGA1860.14684867480913621No Hit
TATCTTGGGGAAATAACCCCAGAGGAGTGAGGGAAAATAACGTGTAGCCAGT1850.146059165804786No Hit
GATATAGGGCAAAGGTATTGAATCAATAAATGAGAAACAAAGGCTTCTCTGG1780.14053260277433463No Hit
GGAATTGGGGTGTAGAAGGGACACTTGCCAAGCACATCATGTCTGAACCTGA1770.13974309376998445No Hit
TCACATGGGCTGGTCCTGGGTAAATAAAAACATGTTGCTTTGTATACATTTT1740.13737456675693385No Hit
GCGGTTGGGCAGTAATGATATTGTGAGTTAGGATAATAACTTTTTTTTTTTT1700.13421653073953307No Hit
ACAGTCGGGATAGTTTGTGAACCCTTGTTCCAGTTGATTTTTTTCCTAGAAA1700.13421653073953307No Hit
CACACGGGACCAAAGGAATTTGCATCCAGCAGCAGCACTTAGACCTGCCAGC1660.13105849472213232No Hit
GGTATAGGGGTGCTGCCCCGGCGGACTTTGAGCCTGCAGCCTGGCGGTGGGG1660.13105849472213232No Hit
TAGCAAGGGCTGTGGGTGATTCGCCTTGAGAGATTACAAAACAAAACATTCT1660.13105849472213232No Hit
GACAATGGATCTCACGTGAAGTGAGCTTCGTTTGTGCTCATTAAAAGTGAGC1640.1294794767134319No Hit
GCTAATGGGGGATGAACATTCCCTCAGGGATTAGGGATAAGAGAAGTTAGCT1640.1294794767134319No Hit
ATGTATGGGAAGACCAACATCGTCACAGCTTCCGTGGACGCCATTAATTTTC1620.12790045870473152No Hit
AATACTGGGAGGTTTTTTTGTCTTTCCTTGCTTGTGTTTTTGGTACTATAGC1590.12553193169168095No Hit
ATGTGAGGGGGTATTAAAAGTGAGTTGAGGACCTAGTGCTATTCATTCAAAA1580.12474242268733075No Hit
GTCAAAGGGGAGGACACGGGTCGCTGGCGGGTTAGGTGGCTGCGTGGCCGCC1580.12474242268733075No Hit
AAAGTAGGGAATTAGCATATAGAAAAATATTACTTTAAGATGACTTGTTTCC1560.12316340467863036No Hit
TATGCTGGGCTCACATCTGCAAAGGGATTTTGCAGATGTGATCAAAGTAAGG1550.12237389567428016No Hit
GTACATGGGATTCATTATCTCTGTAGGAACTTTTAGTTCCATTATATGAACA1540.12158438666992996No Hit
GGCTGTGGACTTTCCAGGGACGCCTCCTGCAGAAGAACAACAAGGACCGCTT1540.12158438666992996No Hit
CGGTCCGGGGGCACTGGCCTTCAACCAAGCCAAGCGCCTTGGCTTCTGTCCT1530.12079487766557978No Hit
CTTGCTGGGAACTCAGGGCTTCCATTTCCTCAACTGTAAAATTGGGAAAATA1530.12079487766557978No Hit
TTGCCTGGGTGCCAGTTTGTATTATAATTGTCTGAACTCATTGACCTACTAT1520.12000536866122959No Hit
GTGATGGGGATTACTTGAGGACTGTGTCTGCTCCGTGAGAGCTGCGTGGGCA1510.11921585965687939No Hit
AAACAAGGGCTCCATTTCTTTATGCCTGGCTTTGCCCCTCTCACCAGCCGTG1510.11921585965687939No Hit
GTATTAGGGAGTGGAGATTTGGTTTATGTTACCATTAAGTTCCTCTAAAAAG1510.11921585965687939No Hit
GCTTCAGGGCATGGCCTGCAGCCTGTTTCAATGCATCTGTCCTTAACCATCC1500.11842635065252918No Hit
TTAGCTGGGTGCACTTATTTTCCAGAAATAAAAATATGAAGAGCTCACGCAG1500.11842635065252918No Hit
CCAAAAGGGGATGCCATGGTTTTACATCTTTTAATTTAAACCTTATACACCA1490.11763684164817899No Hit
ACAAACGGGGGAAAGGATTTGATGAAATGTTCAAGTCAAATGCAGAATAAAG1490.11763684164817899No Hit
GCTAACGGGATACTTTGCTTGGTCCTATAGGCTCTCAGGTTTTTTTGATATC1480.11684733264382881No Hit
CGCCCAGGGAGATGACATGGTTTGTGATGATCCGGAGGCTGGGGAGATGACA1480.11684733264382881No Hit
AACTCAGGGCTCACTGCATCACATAGAATATTATAAAGTTATGGACTCAAAG1480.11684733264382881No Hit
ATGTATGGGAATTTTTTATTGTGGTAACGGACAGGAATAATAACTACATTTT1470.11605782363947861No Hit
TATCGGGGGTCTTCGTCTTAGTCCAGCAAGCACCGCCAATCCTTTTCTTCCC1470.11605782363947861No Hit
TTGCTAGGGGGCTGCCCGAGGGTTCCTCGCAGGCTGGAGCTGACGGCGCTGG1470.11605782363947861No Hit
TTAAGCGGGTGCTGAATTGGGCCTCCTGCTTGGTAACCAAGTAAATGAGCTG1460.11526831463512842No Hit
GCAGCTGGGTACTAGTATTAGTTTTATTAATGCCCTATAAACCCAGCAAGCA1460.11526831463512842No Hit
CATTGTGGGCTACAATTCTGAATTTGCTTTTCTTGCCTCTCTTTAGTCACCT1460.11526831463512842No Hit
TTTCTTGGGAGTGAAAGCAGTGATTAGATACGATTTTATATCCATAAACACC1460.11526831463512842No Hit
TACCTGGGGCATCAGTCCACCCCCATTGTGAATGTTGTCCTGTGTCATCTGT1450.11447880563077821No Hit
GGCGCAGGGGATTGGCTGGTTGCCAAGAAATATATATTTGTCACTAGGGCAT1450.11447880563077821No Hit
TACCTGGGGGAAGCACAAGCAGAGCGGCGGGAGCGTGGGTGCGCTGGAGGAG1450.11447880563077821No Hit
TAGCAGGGGCGGCTTGAGAAGACTGTCAGGAAACGAGTATGTGTTTACTAAG1440.11368929662642802No Hit
GCTGCTGGGATCCGAGGTGCTTGGGAGTTTTGAATGTTAAGAATTGACCATC1440.11368929662642802No Hit
GCAATAGGGGATGTAGGTGTGTGTTTACATGGCTGTTTATTTTTAAGAAATG1440.11368929662642802No Hit
GAGTACGGGTCTGCGAGGGGGACATTAAGCAGCTGTGGCATCTTAGGAATCC1440.11368929662642802No Hit
TTCATAGGGTGCTTAAAGAAAGGCAGGTGCATTGCTCTGAAGTGCATATAGC1440.11368929662642802No Hit
TAACGAGGGAATACCCAACTGTCAATATCAGACAGATCGAGACAGAAAATTA1440.11368929662642802No Hit
CAATTAGGGTGCCTTATGGTAAAATTTACTTAAAACGTTAAACTAAATGAGA1440.11368929662642802No Hit
CTGCATGGGCCAGCCTGGGCAACAGAGTAAGACTCTGTCTCAAAAATGAATG1430.11289978762207784No Hit
AAACGTGGGGATATAGGATTCAGTTCCACAAGAAGACTTAACTATCCTAAAT1430.11289978762207784No Hit
TTCGCAGGGGAGGGCGGCTGCTGCTGCTGGGACGTGCGGCTGGACCAGCCCC1420.11211027861772764No Hit
AGACTAGGGGGAGAAGGGATCTTCTCGAGGTTTGACTTTACTGGCTTCTTAG1420.11211027861772764No Hit
ATCAGCGGGACCAAAGACTTTACCTTTTTGGAATATGCTACCTCTATTTCAA1420.11211027861772764No Hit
CGAGTAGGGAGGCCATGCAAATGAAAATTTTTCCTTCGTCTTACAGATAACT1420.11211027861772764No Hit
GTCTAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1410.11132076961337745No Hit
ATAAGGGGACCAACGATGTAAAAGCATTCCTATTTCTCCACAGCCTCGCTAG1410.11132076961337745No Hit
CCATGAGGGAACTGATTCCATGCACTAGTACATGTAGGCTTCTCCCTTGTGC1400.11053126060902724No Hit
TATAGCGGGTATGTTGTCAGTCCTATTTTATAGATGAGAAAACCAAGGGAAC1400.11053126060902724No Hit
TCGACTGGGGCTCCTGAATGGCTACTGGGTAAATAATGAAATTAAGGCAGAA1390.10974175160467704No Hit
TCAATCGAGGACTCCCTGCTTTGTAGCCGGAGTTTAGGCCGGAGCTTAGAAA1390.10974175160467704No Hit
CGTACTGGGACACAATAAAAAACCAAAACCTGGAAAAGAAATACTTAAAAGA1390.10974175160467704No Hit
GCACAAGGGGACATAGAATGGCTGAATGCATAAAAAGCAAGACTCATTTATC1390.10974175160467704No Hit
TCAAATGGGGATTGCCCAACTGTAAAATATCATGGCTTGAAAAATGCATCCA1390.10974175160467704No Hit
ACCGCTGGCTCTACACATGGGATCACATTCATTCCTCAATCAACTTTGAGGG1380.10895224260032686No Hit
CCTGGTGGTGTTATTAGCCAGAACTCTCTGAAATGTAAATAATTATACAATG1370.10816273359597667No Hit
CTACGTGGGCGATCTCAGGTTGGGGCACCTGAGAAGTAGTTATGTCTAAGTT1370.10816273359597667No Hit
GAAAATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1370.10816273359597667No Hit
GCAACAGGGCACCTTGGTGGTCATTCTTTCTTTGTCTTGAAGAATAGTGCCC1370.10816273359597667No Hit
TCTTATGGGATTCCACAAGGATAAGTGCTTCCTGCTGGGACAAAGTCCTCAT1360.10737322459162647No Hit
CAGCAAGGGATCTTAACGCGGGCTATAAATAAAACATCTTTATCTTATTTGA1350.10658371558727626No Hit
TTGAGCGGGGGGCCGCCGGAGCGCTTTGGAAGGCGCACGGGGCGAAGATGGC1350.10658371558727626No Hit
GTGACCGGGAGACATTGTTTTTCCCTTGAACTGAGCTGTGTGCTGTGTTTTC1350.10658371558727626No Hit
ACTTCGGGGCAAGGGCTTCATGATGGAGCCCGATTGGCCCCTGTACCCGCCG1340.10579420658292607No Hit
CATGCGGTATGTCATGATATCATAAAAGGGACACTGGGAAATCATTTGATTC1330.10500469757857589No Hit
ATCTATGGGTCGCGCCGGGGGTCCAAGAAAGAAGATGTGCCCTGGCCTGCTG1330.10500469757857589No Hit
TAAAAGGGGGTTAATAACTGATTAGGTTATCATGGTGAGTAGCCCTGAGATA1330.10500469757857589No Hit
AAAACTGGGAAGTCTGATAACACTGTGATTACATATGTATCAATATAGTATG1330.10500469757857589No Hit
AGACTAGGGAGGGAAATCTGTCATTTTCCACTGCTTGCTGAAGATGGTTCAG1330.10500469757857589No Hit
TCTCAGGGGAGAAGGAAGAAGAAGAGGGGGAGGAGAAAGAGATAGAAGAGTA1320.1042151885742257No Hit
CGCCCAGGTGAATCTCAATCCTTCCCCCTGAATAGCACGTTTCAACTAGGAA1320.1042151885742257No Hit
AGAGAAAGGGGAAATCTTCTACTTAGAAACATACTAATAAAAGCAATGAATT1320.1042151885742257No Hit
TAGACAGGGCACCGAAGCCTTAAATCCTTCTGTCTTCCTGACTGAATGAAAC1320.1042151885742257No Hit
ATCAAAGGGATTTCATTTGATATTTAGCTTCTGAAAGCCCTTTGTTATAAAG1310.1034256795698755No Hit
GCATTTGGGATTTGATTAAGATAGGTTCTTTCTGTATATGAAACGCTCCCCG1310.1034256795698755No Hit
AATACAGGGATCGACTCGGACACAGAGGACAGCGGCAGCGACGAGAACCTGG1310.1034256795698755No Hit
ACTCAAGGGATATTTTCATCAGAATAATTCACTAAACTCAATCTGAGTCTTT1310.1034256795698755No Hit
AGCATGGGGGTGTAAGACCTATACAAAAACAATAACTACAACAAAACTTTAA1310.1034256795698755No Hit
CGACAAGGGCAGCACTCAGATCACCTCTAGAAATCCCTGTGTGCTTAAATGA1300.10263617056552529No Hit
ATTCTTGGGCATAAATTTGCTACATATAGTTTTCTCTCCTAGATTTATTAGT1300.10263617056552529No Hit
CGCCAGGGATAAAAGGGATATATTTTCTTTAAAGAAAAATGTGAAAATATGA1300.10263617056552529No Hit
GACGATGGGCATCCTTGTCTCTACCAAAAAATTTAAAAATTGGCCGGGCGTG1300.10263617056552529No Hit
ACAGCCGGGAAGGGAGGAGGTGTTCTTGAAGACAGGAGGAGGCTCCCGCTGT1300.10263617056552529No Hit
CATTCCGGGCTACTTCACATCCTCACCAACACATGTATTTTCTAGTTTTTTT1290.1018466615611751No Hit
TGAACTGGGATACTAAAATCATAGTTTGAATTGCTGACATATTAATTGTGGA1290.1018466615611751No Hit
CAACGTGGGAGAGTAAAAGGGGAAATGCCAAGATTTTGGGGTTTTTTTGTTT1290.1018466615611751No Hit
AAAGCCGGGGTAAAAAGTCAAAGCCTCAAAGAAATGGCACAACCATGTTCTT1290.1018466615611751No Hit
GTTGCCGGGCAAAATAAAACGTGATGAACGCACCTTAATAAATCCAGATATT1290.1018466615611751No Hit
TGATGAGGGCTGCTCAGTAAAAATAGCTCATCTTGGTAAGTTGTTTCGACTT1290.1018466615611751No Hit
TAAGTTGGGGAGAGGGGATGTAGGCCCATGGGCACCTGGCCTCCAGGAGTCG1280.10105715255682492No Hit
TCATATGGGGCTTATCAGTCCACCAAGAATTCAGCCCATTTTCTCTCTCTTG1280.10105715255682492No Hit
TCAATTGGGCTGTGGGCATGGGGTAGAGGGGACAGAATGTAGGTAACAGAAA1280.10105715255682492No Hit
CTCATCGGGAGACACCCTCTGGGAAATGCGGCAACCTTAGGGGAAAGGGAGT1280.10105715255682492No Hit
CTTCAAGGGACAGCTTGATGAAGATTTACAAAGTAAACATGCCAGTTGAAAC1280.10105715255682492No Hit
ATGACAGGGCTAATGTGGAAAATTAATTACCTTTATGAAGGTGGTTTATTAC1280.10105715255682492No Hit
AGTAAAGGGGAAACCTTCCTGAACATGCTGTTTCTCAAATTCCTTCTGCTTA1280.10105715255682492No Hit
GTTTTAGGGAAGCATGGTGGGGATCTGGCACAAGGTGTGCCTGGGAAACCCT1270.10026764355247472No Hit
GATTATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1270.10026764355247472No Hit
AGCTGAGGGAGGACAGGCAGAAAGGCGATAATTTGTATCTTTTAATATAAAA1270.10026764355247472No Hit
ATTGTTGGGGAGTATAGAGTTTGGGCAAACCACCTAAACTCTGTGATCTCAA1270.10026764355247472No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)