FastQCFastQC Report
Sat 31 Dec 2022
SRR5846766.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846766.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133022
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGCCGTCT6620.49766204086542076TruSeq Adapter, Index 7 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGC6600.4961585301679421No Hit
CACCACGGGAAAAAATTATGTGAGTAAAATTCAGTTAAGATGACCAGAGGAA1430.10750101486972079No Hit
AGTATCGGGGGCGGCGAGTGAGGCCCCGGTCTTCCTCCTCGTCCTGCCGCAG1410.10599750417224219No Hit
CTGCAGCGGGATTCTGCATCTGGACAGAAAAATAAACCAAGGGAAGAGAGAG1410.10599750417224219No Hit
AGCAATGGGCTACCAATGCCAAAAGGATAAATGTCTTTCCAAAAGTGTGTAT1400.10524574882350288No Hit
GCATTGGGGATACACACAAACTAGAAACTCTAGAGAAAAATGGATAAATTCC1390.10449399347476358No Hit
TGAAATGGGATTGGGAGAGGTGGCTGTTTTTCAAATGCAAAAATCACAACAA1380.10374223812602426No Hit
TGAAATGGGCCTCAGGCAGTTCTTTAGGAAAACTGTCAGCATCATGGGTACT1350.10148697207980634No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)