FastQCFastQC Report
Sat 31 Dec 2022
SRR5846765.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846765.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132793
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGCCGTCT6300.4744225975766795TruSeq Adapter, Index 7 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGC5710.4299925447877524No Hit
AGTATCGGGGGCGGCGAGTGAGGCCCCGGTCTTCCTCCTCGTCCTGCCGCAG1470.11069860610122523No Hit
CAATATGGGTATCCTGCCAGAATAAGCCTTATAGATAAAGGGGAAATAATCT1460.10994555435904002No Hit
GCTTGTGGGTCAACAGAGTAAACAGACAACCTATGGAATGGGAGAAAATATT1430.1076863991324844No Hit
GGCGCCGGGATCTTTTAACCAACTGAACAATACACCAAAAGCAGCCTAGGGA1430.1076863991324844No Hit
CACCACGGGAAAAAATTATGTGAGTAAAATTCAGTTAAGATGACCAGAGGAA1430.1076863991324844No Hit
GATCTCGGGCACCCACCTCCCTCCCCTCCTGTCCATTTCAGTGAGGTTTTCA1350.10166198519500275No Hit
TGTTATGGGCTTCTTATTGTATTCAAACGGTGTTGCAACTCACAAGTAAAGG1350.10166198519500275No Hit
TCTCCAGGGTAACTACTTGAAGGAACCTCAGCTATGATTTGGTCCAACCTCC1330.10015588171063235No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)