FastQCFastQC Report
Sat 31 Dec 2022
SRR5846758.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846758.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165714
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGC7530.4543973351678192No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGCCGTCT7200.43448350773018574TruSeq Adapter, Index 7 (95% over 21bp)
GAGTCATCTGACACTGATGATGAAGAATTACGGCATCGAATCCGGCAAAAAC2680.1617244167662358No Hit
ATAAGCGGGATCTTGGCATAACATGGTTCTTTTTTAAATAGAAACTTTAGTT2130.12853470437017994No Hit
AGACCAGGGATCTAGTCATGGGCGATCTTTTGATGGGAGGCTTTGTCTGGAA2120.12793125505388803No Hit
TGCGCGGGGAGTATCTGCCATGGAAATATTTCTGTTTTTAGATATTTTTGTG2060.12431055915613648No Hit
ATCATGGGGGTCTCGGTCCAGGCCTTCTGCGAAACCGTGGAGACCGCCGCCG2030.1225002112072607No Hit
GGAAATTTTACTTGGGGACCAGGAGGATTTGAAAGGCTGCATGTACTCAGAA1960.11827606599321722No Hit
GGTTGCGGGAACACAGGGTGTTTAGCAAACATTTAGTTATTTCCTTAAGCCA1920.11586226872804953No Hit
TTCGCGGGAGCGTCTAATGAGCTAATTTTTGTCCAGTGATCACATCTTGAGA1900.11465537009546567No Hit
TGACTGGGGGGGGCCTAGGCTCCAGAACTCATACAATGTCAAGTGTGCCACG1880.11344847146288184No Hit
TAAATAGGGAACATAATTGTTTATTTTCCTAGGGTAGATAAAAATAGCTTTT1840.11103467419771414No Hit
GATAATGGGAAGTTACTTAAAATCTCTAAGTCTCAGTTCTGTCATCTAGAAA1810.10922432624883836No Hit
TACATTGGGGACGACGACGAAGAAGCAGGAGTGGCACAAGATCTCCTAAAAA1790.1080174276162545No Hit
AACGCGTGGGAGATTAAACAAAAAATACTATAAAACCCCTGACCACATTTAA1790.1080174276162545No Hit
TGCCAAACTGTTTTTGCAAAGTAGTTGCTCCATTTTGCATCCCGTCGGCAAC1780.10741397829996259No Hit
ACGCTAGGGTGCTGTAAAGATGGTTTTGCAAGAATAAGTCATCACCAAGACA1770.10681052898367067No Hit
TTTGATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1750.10560363035108682No Hit
TTGCCAGGGAAGGAACCCCGAGGCCGGTGTGGCAACCACAGATTTGTACGGC1750.10560363035108682No Hit
TCCGCTGGGATTCTCACATCCTCCAAAGTGCTTTCTTCACATTGCTTTGTAG1740.1050001810347949No Hit
ATCCGCGGGATTTGACAAAGATGGTGATGGAACTATAACAACAAAGGAATTG1730.10439673171850296No Hit
CTTCTAGGGCATAGACCTAATTTGCAAACTCAATCGGGGACTAAAATTTCCC1720.10379328240221104No Hit
GTTGCAGGGGTCTCCAAGGGCCGGTTCTTCAGCACCAGAGCCCGAAGAGCCC1720.10379328240221104No Hit
ATAGCAGGGCGACTGTGTCATGACAAGGAAACTTACAAACTGCAACGCAGAG1720.10379328240221104No Hit
AAAAGGGGGTGACAGAGAAAGGAGGCAAACCAGCTTGTCTACTTCTTAAACT1720.10379328240221104No Hit
GGCAAAGGGAGCTGCAGCTAAGGAAGCATGACCACAAAACCAATAAAAAAGA1720.10379328240221104No Hit
TTAAAGGGGCGATTGATATTTATCTGATGGGTTGCTTTGTGTTTGTGTTCCT1710.10318983308591913No Hit
CACTTAGGGCGTTTGAGCAAGGAAGACATTGAACGTATGGTCCAGGAAGCTG1710.10318983308591913No Hit
GATTACGGGGAGCCCTGCGAGCAATAGAGTTGAAGCAGCCTCTGCTGGACAG1700.10258638376962718No Hit
GACAGTGGGCTCTTAAATCCAGAAAGTTATATTTTGCCGGAAGGAAGAAGAA1700.10258638376962718No Hit
TAGTAGGGGCTGACAGTTCACCAAATAGCTAGTCATGGAGAATGCAGGCAGT1690.10198293445333527No Hit
GTACGAGGGATATAATCCAGTAATCATGGTCCACAAGAACCTGCATACAGAT1690.10198293445333527No Hit
TTCAGCTGGGAATGACAAGGAAAAAAAAGCCTGTACATGTTCAGCACAAGTG1690.10198293445333527No Hit
GTGCGCTCGGGAGACTTCCAGATGGTCTCCCGAACCGCCTGGCTCTGCCGGC1690.10198293445333527No Hit
TGTGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1690.10198293445333527No Hit
GTAATAGGGGAAGGCTGAATCAAAGACATTTCATCCACCAATATCATGTGTA1690.10198293445333527No Hit
GCACTGCGGGATCTTGGAATTCAGTAAGTGCATATCCTAACTTGCCCATATT1680.10137948513704333No Hit
TACGTCGGGACTGACATTGTTAAACAGGTCTGTCTGGAGTGGGTGGAGCTGG1670.10077603582075141No Hit
TTTATTGGGTATGACTGAGATTTATTTCTCTCTTTTAGATGAAATTGTTGAT1670.10077603582075141No Hit
TCACTGGGGATTCAGCCTCAGTAGTAGCGAACTGCACTGCTGTTTGGTTTGG1660.1001725865044595No Hit
TTCCTGGGGCCCGACATCCATTCTCTGGTACAGTCAGACCAAGAAGCTCAGG1660.1001725865044595No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)