FastQCFastQC Report
Sat 31 Dec 2022
SRR5846756.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846756.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163173
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGC7770.47618172124064645No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGCCGTCT6970.42715400219399047TruSeq Adapter, Index 7 (95% over 21bp)
GAGTCATCTGACACTGATGATGAAGAATTACGGCATCGAATCCGGCAAAAAC2800.17159701666329602No Hit
ATCATGGGGGTCTCGGTCCAGGCCTTCTGCGAAACCGTGGAGACCGCCGCCG2150.131761994937888No Hit
GGTTGCGGGAACACAGGGTGTTTAGCAAACATTTAGTTATTTCCTTAAGCCA2070.1268592230332224No Hit
ATAAGCGGGATCTTGGCATAACATGGTTCTTTTTTAAATAGAAACTTTAGTT2050.12563353005705602No Hit
GACAGTGGGCTCTTAAATCCAGAAAGTTATATTTTGCCGGAAGGAAGAAGAA1900.116440832735808No Hit
GGAAATTTTACTTGGGGACCAGGAGGATTTGAAAGGCTGCATGTACTCAGAA1900.116440832735808No Hit
AGTAGCGGGACGAAGTTTACAGTTTCATACCAACTATCTAAAACCTAATTGC1840.11276375380730881No Hit
TATCAAGGGTATTACAATTTAGTTTTCAGTAATCAAAAAGATTTCTATGAAT1840.11276375380730881No Hit
TGACTGGGGGGGGCCTAGGCTCCAGAACTCATACAATGTCAAGTGTGCCACG1780.1090866748788096No Hit
AGGCAAGGGCATTGATTACTAAATCAGAATTCATTTAGCTCACCACATCTCT1770.1084738283907264No Hit
TGCGCGGGGAGTATCTGCCATGGAAATATTTCTGTTTTTAGATATTTTTGTG1760.1078609819026432No Hit
TGCTTAAGGGGGATCCTGGCTCTGCAATGTAGTTACACAACCTGGACAAGTT1740.10663528892647682No Hit
TGAGGGGGAAGGTAGAACCTACCCTGAACACTTGATTTGAATATTTCTATTC1730.1060224424383936No Hit
GCCTGTGGGATTCATCTTATCTGTAAAATGATAGATTATTTTATCACAAAGG1730.1060224424383936No Hit
AGACCAGGGATCTAGTCATGGGCGATCTTTTGATGGGAGGCTTTGTCTGGAA1720.10540959595031041No Hit
GTTGCAGGGGTCTCCAAGGGCCGGTTCTTCAGCACCAGAGCCCGAAGAGCCC1710.10479674946222721No Hit
TCTGCTGGTACATCCAGCGGCTCCTCCGCGCTGAGCAGGAACGGTTCCTTTA1710.10479674946222721No Hit
TGTGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1700.10418390297414401No Hit
GGTTATGGGCTGCTGATATAAACTTACATAACCATTAATATTCAATCCTCTT1680.1029582099979776No Hit
TTAAAGGGGCGATTGATATTTATCTGATGGGTTGCTTTGTGTTTGTGTTCCT1670.1023453635098944No Hit
CTTCTAGGGCATAGACCTAATTTGCAAACTCAATCGGGGACTAAAATTTCCC1670.1023453635098944No Hit
TTTATTGGGTATGACTGAGATTTATTTCTCTCTTTTAGATGAAATTGTTGAT1670.1023453635098944No Hit
TTTCAAGGGGAAAAAACAATTCTTGGTAATTTGTCTATCTGGTTTAAATTTT1670.1023453635098944No Hit
GGGACAGGGAATTGCTTAAACCCTTAAACCCGGGAGGTGGAGGTTGCAGTGA1660.10173251702181121No Hit
TAGTAGGGGCTGACAGTTCACCAAATAGCTAGTCATGGAGAATGCAGGCAGT1650.10111967053372801No Hit
TTCGCGGGAGCGTCTAATGAGCTAATTTTTGTCCAGTGATCACATCTTGAGA1650.10111967053372801No Hit
GAAATTGGGATACCAACTCAACTTTTTTCCTGTGAACACATAAATATATTTT1650.10111967053372801No Hit
CACCGGGGGGTCACTAAATGGTGAGATCATCAAGCCATTTTAAGTTCTTTCT1650.10111967053372801No Hit
AATAGCGGGTAATGATGATCCACAGTGTGTATTTTACTATTGGGACAGATAA1640.1005068240456448No Hit
CACTTAGGGCGTTTGAGCAAGGAAGACATTGAACGTATGGTCCAGGAAGCTG1640.1005068240456448No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)