FastQCFastQC Report
Sat 31 Dec 2022
SRR5846755.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846755.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154289
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGCCGTCT12690.8224824841693186TruSeq Adapter, Index 7 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGC11920.7725761395822126No Hit
ACAAACGGGACTCCCCTTCAGCCCCCCGGCTGATGCCATCTCTGGTTCTGGA2350.1523115711424664No Hit
TGCTCTGGGTAAAACCCTGGAGGAGCTGTTCTGGTTCCTTTGAAAAATAGAC2150.1393488842367246No Hit
GTTCTCGGGCAACAGAAAATACATTCTTCTCAGCACCACATTGTATTTGTTC2140.1387007498914375No Hit
ATGTGGGGATGGATATGTAGTAAAATTATGAAAATATGTATGAGGATAAGTA2130.13805261554615042No Hit
TACCACCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG2080.13481194381971495No Hit
TCTTTTGGGATACAAAAGCAAAGCATTTGATAACTTCAACAAAATTTTTCTG2070.13416380947442785No Hit
GCGGCCGGGGGAGAAAAAAATACAAAACTAATCATAAAATTTTGCCTAAGGA2050.13286754078385365No Hit
TGCAATGGGGAGTCTTCTGACCAAAAGACTTGAAGGAGGGCGATGTGTTTAT2030.13157127209327948No Hit
GTAATTGGGTGCGCAGAGCGGTTTGGTCGTTCGTTGGGCGGTGCTGGTTTTT2030.13157127209327948No Hit
GTGATTGGGCATTCTAAATGTCAAATCTGACAATTGGAAAACAGTACAGTTT2000.12962686905741821No Hit
AAGGCCGTGGAGGAGCTAAGGACACAGGTCCGCGAGCTGAGGAGCATCATCG2000.12962686905741821No Hit
GTAGAGGGGGCTGCCTATAGTGTCTTTTGGAAAACTACTTAAAGGGTGATTT1970.12768246602155695No Hit
GTAGGCGGGACAATTTTGATTTTATATTGCTTGTTATAATATTAAATAACAG1960.12703433167626985No Hit
TGTAGAAGGGAAGTGACTTCTGATTTCTACTTTAAAAAATCACCAAGGGAAG1950.12638619733098277No Hit
ACTCGTGGGCAGTGGAACAGGAAATTGATTTAAAAGATGTTTTGAAGGCTCT1940.12573806298569568No Hit
TAATACGGGACCTCTCTCTGCCTTCCAACCTGAAGACGCCCATCACGCTCTT1940.12573806298569568No Hit
CACGATGGGAGCCAGATCAGTTGATTAAGTGTCCCCGCCGGAACCAGCCAGT1920.1244417942951215No Hit
ATCTCGGGGGTTGTCACAGAGGCAAGTGCTACCGCACAGGCATGCTGCAGTG1900.1231455256045473No Hit
GCTCAAGTCAAAAAACTCCGGAAGCAACAGCTTCCACCTTCAAGCAATCCAC1890.12249739125926022No Hit
GATATTGGGACCCGGAAGCAGGGGCCCGAGCGGAGCCGGCCGCGATGAGCGG1890.12249739125926022No Hit
ATTGGCGGGGAGAGTGATCTTAAATATGGCAAGATACAGCTCATTTAGAATA1880.12184925691397312No Hit
GAAAACGGGATGAGTGAAAAGCGGGAATTTCCTACAGTGCCCTTGGTTAAAT1870.12120112256868602No Hit
CGGCTTGGGGTGGGACTGAACTGATGAATAATTGAAATGATTTTTATATTTT1860.12055298822339895No Hit
TAACTAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1860.12055298822339895No Hit
AATTTAGGGAACTACTCAGTGCTGAAGCAGTGTCAGAATTTATAGGCCTCTT1850.11990485387811185No Hit
GGGCACGGGAAGAACACAAGACTTCTACATGTTGGGTAATACCTAGAGACGC1840.11925671953282475No Hit
GACAAAGGGTACTGTTCACTAAGGGAGGGAATATGTGGTTTATTTATTGAAT1840.11925671953282475No Hit
CAGAATGGGTGCCTGTAGCATGACCTGGAATTCTGTGTAATTACTGCTTTCA1840.11925671953282475No Hit
AGCAAGGAAGGCCAACATTCAAATTCAGGAAATACAGAGAACACCACAAAGA1830.11860858518753768No Hit
ACCTAGGGATTACTGTGGACAGATTATTTGAGTTCTGTCATTTGAACATTTT1830.11860858518753768No Hit
TAACTAGGGGGGGGAAGGAGCAGCACCAAATCCAAGATGGCGGCCAGCAGGA1830.11860858518753768No Hit
ATTTGAGGGGGAGCATCTGCTTTTTGTCTTTATCTTTCAAAAGGTTTTTGCT1820.11796045084225058No Hit
AACGCAGGGATTCTACAACATCATACTACACAATAATAAGACATATAGACTT1820.11796045084225058No Hit
TGTGAGGGGATCAGAGTCACAGGCTTTGACTGACCAATGCAGTTAGAAACTT1820.11796045084225058No Hit
AGCGCGGGGTATTCAAAGCCTTTTTAAAGGAACCAACTACTCAAATGCTCTA1810.11731231649696348No Hit
GCAATAGGGAAGGCAGAGGCCTGCCCTGCTGCTGCTGGCGCTGTCACCTCCC1790.11601604780638931No Hit
CTGTGCGGGATCAATACAACTGATTTTCCTCCATCCGTTGTATTATTTAGCA1790.11601604780638931No Hit
TATGCTGGGACGTTCACACTATTTGGTTCCAAACTATTTTTTTTTAATTTGG1780.11536791346110221No Hit
GTGCAGGGGAAGACATGGAAGGTCGCATTTAGGAGCTCCCACCAAGATGTCT1780.11536791346110221No Hit
ACACCAGGGTTTCAATGTAGATCTTTAAGAGAAATCCTTTTCAAGAGTCGGA1780.11536791346110221No Hit
AAAGGTGGGCATCCTCACATGTGGCACGTAAGGTCTCATTTGATATTGTGTA1780.11536791346110221No Hit
CATTTAGGGATTGGATAAAGTGAGTGCCTTTGGAAGAAAGGAAGCTGATTTT1780.11536791346110221No Hit
AATGACGGGGAAAGAATGAAGTTAAATTGTCTCTGTAGATGACATGATCTTA1770.11471977911581513No Hit
TTGCTAGGGACTTAGCACTTGGCCTGAGATGCCTGGTGAGCATTACAGGCAA1770.11471977911581513No Hit
TCAGCAGGGGATGAAAGTGTCACGGGTAAAAACTCTAAAAGGTTAATTTCTG1750.11342351042524093No Hit
TTTGGCGGGTCTACTGAAGGGGAGCCCCGAACAGAGTGAAGAGTCTTTGGTG1750.11342351042524093No Hit
AGTAGAGGGATGACTAATCACATATCCTGTTTCTGTAGTGACAAGTATTTAT1740.11277537607995386No Hit
CTCCTCGGGAACATACTGAAACTATTTATATTTTCTTTTTTAAGGATATTTA1740.11277537607995386No Hit
AGCATAGGGGTGGGGAAGATATTCAGTTTATAATAGATGAGATTACACTAAT1740.11277537607995386No Hit
GAAAACGGGATTTCTCTTAAACCAGGATTCTTTAAATGTTATTAACCAAGAG1740.11277537607995386No Hit
CTTCACGGGGCTGAAAACAATAATCAAATATGAACATAAGAGATAACAGGTA1740.11277537607995386No Hit
GTACAGGGGGAATTAGAATTAAAATGGTACATTATGAAATATTTATTTAACA1730.11212724173466676No Hit
AACAATGGGAGACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTT1730.11212724173466676No Hit
CTGCTACCGGGCATACACTTGCTATATAGTAGTATGCAGTATTTATATTATT1730.11212724173466676No Hit
TGTTTGGGGACTGAACCACAAACCTAACCTCATTTTTTAAAAACTGAAGTGC1730.11212724173466676No Hit
GCATAAGGGGCTTCGTAAGGATGATGCCGAAAGGGAACTGGGGTCTCCATGG1720.11147910738937966No Hit
TCAAAAGGGCTAAGCAAGGGATGTTAATGTTCTCTGTTTATAAAAATTGAGT1720.11147910738937966No Hit
ATACATGGGCAATCTTTCTAATGGTGGAACTTTTTAATATAAAGTTTTTATT1720.11147910738937966No Hit
ACGCAGGGGCAGAAATCCTCTGTGACGATCTGGATTTGAACCCGCTGACGTT1710.11083097304409259No Hit
GTTCATGGGAAGTTGCAAGATCTTATTTATTAACTAGGTATGAAGTATAAAC1710.11083097304409259No Hit
ACATAAGGGGTTTTTATATATGGTGTTAGATAAGGGTCCAACTTCATTCTTT1710.11083097304409259No Hit
CGTGCAGGGAGCCTTGGCGGAGGGAGAGTGCTATTTGCTTTTGTTCTCCCTC1700.11018283869880549No Hit
CGACATGGGCACCCTCCAAGTAATCTTTGAGGTGGCCAGTGCTGGCAGGCGC1690.10953470435351839No Hit
CAACAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1690.10953470435351839No Hit
ACATGCGGGAAGTAATTGTGGGCTAAGTGAACTTAAACCACGTCGTGGACTC1690.10953470435351839No Hit
TGGCCTAGGGTGCGAAAGGTTTCCGTTTCAGAAATACAATGCCCTGATTTTA1690.10953470435351839No Hit
ATACCCGGGTATCTTAGTTTGCTACCAGCATCCAGCATGAAGTTGTAAAGTG1680.10888657000823132No Hit
CATCTGGGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAAT1680.10888657000823132No Hit
TCTCAAGGGGCTGGACAGCAAACATGGAGAGAATCATGAAAGCTCAAGCCCT1680.10888657000823132No Hit
TGTTAAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1680.10888657000823132No Hit
CTACAAGGGAGGAGGGAAGATGGCGGACGAGGAGAAGCTGCCGCCCGGCTGG1670.10823843566294422No Hit
CACATAGGGCACATGCAAGCATCCCCGTTCCAGTGAGTTCACCCTCTAAATC1670.10823843566294422No Hit
GACTATGGGAGTAATGAGCATGTAATAGTGATATATTAACATGTTAAATAAT1660.10759030131765712No Hit
TGCATCGGGCAGCAAGGCCAAGGTGGAGTCTGAAGAGCCACAGGACAATGAC1660.10759030131765712No Hit
ATCAGAGGATTCGCTTATTGATTTATTGTACACATCCATTTTCTGTACCACC1660.10759030131765712No Hit
AACTGTGGGCATCCTCATCGACATGGTCCTGAACCGCATGGCAAAAGGAGTG1660.10759030131765712No Hit
ACTACTGGGGGGAAAATCGGTTCTTCCAAATTTTCTATATTCGATTATCAGG1660.10759030131765712No Hit
TTCTAGGGGTGTTTTCAACTCTCTATTTAAACATACATTGGACCTAATCACT1650.10694216697237004No Hit
ACAAAGGGCAGCTTCCCAACAATACTTTGTGTCTTTTGTTCTCTCTTGGTCT1650.10694216697237004No Hit
TGCGAGGGCTGCCGGTACCACCCTTTGGATTATTGTAGTGATATAGTAAGCT1640.10629403262708294No Hit
AAATGAGGGTTCCTGACCTCGTGATCCGTCCGCCTCGGCATCCCAAAGTGTT1630.10564589828179584No Hit
AAACGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1620.10499776393650877No Hit
CCACAAGGGTACCAACACAAGATTAAAGGTTTAAAAATCTCTTACGTGTACA1620.10499776393650877No Hit
TGATACGGGATTATTATTTGCTAAGAAATTCTGTCAGGGCTTTGCCTTCATA1620.10499776393650877No Hit
TTAGATGGGTACATGATCAACGCAGTGACTCTTAACAACCTGCTCTTGCGGA1620.10499776393650877No Hit
AGGCTTGGGCTTCACTCGGGGCATTGAATTACTTTTCTTGTATAATGTTTTG1620.10499776393650877No Hit
TTTACTGGGTTTGATGGTGAAGAGAAAATGAGATGGAGGTCACTTACTGAAT1610.10434962959122167No Hit
GCTCACATCTGAATCTCCTTGTTGTTCTTTAACCCTCACTGGGACTTTGTAA1610.10434962959122167No Hit
ACATGCGGGGAGCGGCGGGGTCGACTGACGGTAACGGGGCAGAGAGGCTGTT1610.10434962959122167No Hit
GCACATGGGCGATCTGCTTAGGGGAACAGAAAGCTAGTGATAAATGCTGTTC1610.10434962959122167No Hit
CCTAAAGGGATTGTGTTTCGAACTCCACATTGCAATACTCTGTTGTCATGGA1610.10434962959122167No Hit
GTTGTTGGGGGCACCAAAGGTACCAACCATGAAGAAAAGAACTGATAAGCCA1610.10434962959122167No Hit
TCAAAGAGGGGTTTTTACGGCGCCGTAAAGCAGCCCGGCCGAGCAGACTGCT1600.10370149524593457No Hit
GAAAAAAGGGATAGATTATTATTGTACACAGGTAGTGTATTTTTATCCCCAT1600.10370149524593457No Hit
AAATAAGGGTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAA1600.10370149524593457No Hit
ATAGCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1600.10370149524593457No Hit
TAAGTGGGGAGTCTCAGAATACAAAATCAATGTGCAAAAATCACAAGCATTC1600.10370149524593457No Hit
TTGTCAGGGGGGTGCCTGTAGCGGCGATGGCAGCTGAGGCAGCGGCGACCTC1600.10370149524593457No Hit
AGTATGGGGATGTCATACAATTTATTTGGCATGTCTTGTTTCAATACATCCT1600.10370149524593457No Hit
AGCCATGGGAGACATCAGATAATCCATACTGGAAAGAAACCTTACAAATGTA1590.1030533609006475No Hit
TTTTGTGGGGTACAGAGATTATAAAGAAGAAATATAGCTCACAAAGGATGAG1590.1030533609006475No Hit
AGAGAAGGGATACTAAAGCATTCATTAAAAATACAACTAATTCAAGGACAAG1590.1030533609006475No Hit
GGTGCCGGGAGGAGAGACAGTCTGTAAATATAACGAATGTGGAAGAACCTTC1580.1024052265553604No Hit
GCCATAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1580.1024052265553604No Hit
CTATATTGGGGACAGCAGCGATGACAGTATAGGCAACTACCACAAGAAAGAA1580.1024052265553604No Hit
TATCGCGGGAAACTTTGTTACTGAAGAAGACAAGAGGATTCTTCGTGACATT1580.1024052265553604No Hit
AACATCGGGATATCAACACACCCAGTTAATTTTTTTTGTTTGTTTATTTTTG1570.1017570922100733No Hit
GTGGATGGGACAGCCGGGGAGGCGGGGTCCTTCTCTGGGGCGGTCGCGTTGG1570.1017570922100733No Hit
ATCAGAGGGACCTTCTTCTCTTAATTGCTTTTCATGTAATCATGATAATTTA1570.1017570922100733No Hit
AACTTGGGGCGTTGAACTGGGGCAAGTACTCTGTAACACAAAGTGGGAAACC1570.1017570922100733No Hit
GGTGGGATTGGTGTTTAAAAATATTGATTTGCAGATGTTCACAGAACACTTG1560.10110895786478623No Hit
GTTTACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1550.10046082351949913No Hit
GCATATGGGTGGCCCAGCCGCTCGCTCGGCTCCGCTCCCTGGCTCGGCTCCC1550.10046082351949913No Hit
AAGCATGGGATATTCTTGAATGAAAAAGATCCACATTTCATCATTTGCCTGT1550.10046082351949913No Hit
GCACCTGGCTGTCTGATTCCATTTTAAAAAGAAAAGAAAACTCTTTGTTTTG1550.10046082351949913No Hit
CGCAACGGGACAGGAAAACAGTACTCTTGATCCAGCAAATGTTTGATAGATT1550.10046082351949913No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)