FastQCFastQC Report
Sat 31 Dec 2022
SRR5846754.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846754.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153834
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGCCGTCT12290.7989131141360166TruSeq Adapter, Index 7 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGC11620.7553596734141997No Hit
TGTAGAAGGGAAGTGACTTCTGATTTCTACTTTAAAAAATCACCAAGGGAAG2260.14691160601687533No Hit
GTTCTCGGGCAACAGAAAATACATTCTTCTCAGCACCACATTGTATTTGTTC2240.14561150330876138No Hit
ATGTGGGGATGGATATGTAGTAAAATTATGAAAATATGTATGAGGATAAGTA2240.14561150330876138No Hit
ACAAACGGGACTCCCCTTCAGCCCCCCGGCTGATGCCATCTCTGGTTCTGGA2200.14301129789253353No Hit
ATCTCGGGGGTTGTCACAGAGGCAAGTGCTACCGCACAGGCATGCTGCAGTG2160.14041109247630565No Hit
TGCTCTGGGTAAAACCCTGGAGGAGCTGTTCTGGTTCCTTTGAAAAATAGAC2130.1384609384141347No Hit
AATGACGGGGAAAGAATGAAGTTAAATTGTCTCTGTAGATGACATGATCTTA2120.13781088706007774No Hit
GTAGAGGGGGCTGCCTATAGTGTCTTTTGGAAAACTACTTAAAGGGTGATTT2110.13716083570602078No Hit
AGCAAGGAAGGCCAACATTCAAATTCAGGAAATACAGAGAACACCACAAAGA2040.13261047622762198No Hit
CCACAAGGGTACCAACACAAGATTAAAGGTTTAAAAATCTCTTACGTGTACA1990.12936021945733714No Hit
TAATACGGGACCTCTCTCTGCCTTCCAACCTGAAGACGCCCATCACGCTCTT1980.12871016810328018No Hit
CAGAATGGGTGCCTGTAGCATGACCTGGAATTCTGTGTAATTACTGCTTTCA1970.12806011674922319No Hit
GCTCAAGTCAAAAAACTCCGGAAGCAACAGCTTCCACCTTCAAGCAATCCAC1940.1261099626870523No Hit
TTCTAGGGGTGTTTTCAACTCTCTATTTAAACATACATTGGACCTAATCACT1940.1261099626870523No Hit
TACCACCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1940.1261099626870523No Hit
AAGGCCGTGGAGGAGCTAAGGACACAGGTCCGCGAGCTGAGGAGCATCATCG1930.1254599113329953No Hit
CTTCACGGGGCTGAAAACAATAATCAAATATGAACATAAGAGATAACAGGTA1930.1254599113329953No Hit
GTAGGCGGGACAATTTTGATTTTATATTGCTTGTTATAATATTAAATAACAG1930.1254599113329953No Hit
CAACAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1920.12480985997893833No Hit
GTAATTGGGTGCGCAGAGCGGTTTGGTCGTTCGTTGGGCGGTGCTGGTTTTT1920.12480985997893833No Hit
ACATGCGGGAAGTAATTGTGGGCTAAGTGAACTTAAACCACGTCGTGGACTC1920.12480985997893833No Hit
TAAGTGGGGAGTCTCAGAATACAAAATCAATGTGCAAAAATCACAAGCATTC1920.12480985997893833No Hit
ATTTGAGGGGGAGCATCTGCTTTTTGTCTTTATCTTTCAAAAGGTTTTTGCT1910.12415980862488137No Hit
GCGGCCGGGGGAGAAAAAAATACAAAACTAATCATAAAATTTTGCCTAAGGA1910.12415980862488137No Hit
CAGTGCGGGAAATACTTACCATGTGACTTTTTATTACCAAATGCATCAGTAG1910.12415980862488137No Hit
TCTTTTGGGATACAAAAGCAAAGCATTTGATAACTTCAACAAAATTTTTCTG1870.12155960320865349No Hit
TGCAATGGGGAGTCTTCTGACCAAAAGACTTGAAGGAGGGCGATGTGTTTAT1860.12090955185459651No Hit
AAGCATGGGATATTCTTGAATGAAAAAGATCCACATTTCATCATTTGCCTGT1860.12090955185459651No Hit
TGACTGGGGATTGTGAACTCTAGCTTTGATCATGGTAAAAAGTTAACCTTTT1850.12025950050053955No Hit
GTGCATGGGCAGGTTTACAAACTCAGACCCTTTTAAAAGTTGTTTAAATTGT1850.12025950050053955No Hit
GAAAACGGGATGAGTGAAAAGCGGGAATTTCCTACAGTGCCCTTGGTTAAAT1840.11960944914648257No Hit
AACATCGGGATATCAACACACCCAGTTAATTTTTTTTGTTTGTTTATTTTTG1820.11830934643836863No Hit
AACAATGGGAGACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTT1820.11830934643836863No Hit
TTTGGCGGGTCTACTGAAGGGGAGCCCCGAACAGAGTGAAGAGTCTTTGGTG1820.11830934643836863No Hit
ACATGCGGGGAGCGGCGGGGTCGACTGACGGTAACGGGGCAGAGAGGCTGTT1820.11830934643836863No Hit
GTTGATGGGAACCTCTAAAGGTGTTGCTTACATTGTCTTTTAATATTGAGCA1820.11830934643836863No Hit
GGGCACGGGAAGAACACAAGACTTCTACATGTTGGGTAATACCTAGAGACGC1810.11765929508431167No Hit
AGCGCGGGGTATTCAAAGCCTTTTTAAAGGAACCAACTACTCAAATGCTCTA1800.1170092437302547No Hit
AGAGAAGGGATACTAAAGCATTCATTAAAAATACAACTAATTCAAGGACAAG1800.1170092437302547No Hit
AACTGTGGGCATCCTCATCGACATGGTCCTGAACCGCATGGCAAAAGGAGTG1800.1170092437302547No Hit
TCAAAGAGGGGTTTTTACGGCGCCGTAAAGCAGCCCGGCCGAGCAGACTGCT1790.11635919237619771No Hit
TCAGCAGGGGATGAAAGTGTCACGGGTAAAAACTCTAAAAGGTTAATTTCTG1790.11635919237619771No Hit
GATATGGGACCTATATTTACTCATTAAACAAAATTTTAAAAATAAGACAAGA1790.11635919237619771No Hit
ATCAGAGGGACCTTCTTCTCTTAATTGCTTTTCATGTAATCATGATAATTTA1780.11570914102214075No Hit
TCTCAAGGGGCTGGACAGCAAACATGGAGAGAATCATGAAAGCTCAAGCCCT1780.11570914102214075No Hit
ATACATGGGCAATCTTTCTAATGGTGGAACTTTTTAATATAAAGTTTTTATT1770.11505908966808379No Hit
GACAAAGGGTACTGTTCACTAAGGGAGGGAATATGTGGTTTATTTATTGAAT1770.11505908966808379No Hit
AATTTAGGGAACTACTCAGTGCTGAAGCAGTGTCAGAATTTATAGGCCTCTT1760.11440903831402682No Hit
GTTCATGGGAAGTTGCAAGATCTTATTTATTAACTAGGTATGAAGTATAAAC1760.11440903831402682No Hit
GTTGTTGGGGGCACCAAAGGTACCAACCATGAAGAAAAGAACTGATAAGCCA1760.11440903831402682No Hit
GTGATTGGGCATTCTAAATGTCAAATCTGACAATTGGAAAACAGTACAGTTT1750.11375898695996983No Hit
GCAATAGGGAAGGCAGAGGCCTGCCCTGCTGCTGCTGGCGCTGTCACCTCCC1750.11375898695996983No Hit
TGTTTGGGGACTGAACCACAAACCTAACCTCATTTTTTAAAAACTGAAGTGC1750.11375898695996983No Hit
GCACCTGGCTGTCTGATTCCATTTTAAAAAGAAAAGAAAACTCTTTGTTTTG1740.11310893560591287No Hit
AAAGGTGGGCATCCTCACATGTGGCACGTAAGGTCTCATTTGATATTGTGTA1740.11310893560591287No Hit
CGCAACGGGACAGGAAAACAGTACTCTTGATCCAGCAAATGTTTGATAGATT1730.11245888425185589No Hit
GCTATTGGGAATGCTGGACAGCTCCTTGACACAGGGCTGCTTTTCTTTGGTT1730.11245888425185589No Hit
CGACATGGGCACCCTCCAAGTAATCTTTGAGGTGGCCAGTGCTGGCAGGCGC1720.11180883289779893No Hit
ACTTGAGGGGCCTCTGTCATGGGCAGCATATTTAACAGAATAAATTCTAAGC1720.11180883289779893No Hit
CACGATGGGAGCCAGATCAGTTGATTAAGTGTCCCCGCCGGAACCAGCCAGT1720.11180883289779893No Hit
CGTGCAGGGAGCCTTGGCGGAGGGAGAGTGCTATTTGCTTTTGTTCTCCCTC1710.11115878154374197No Hit
GTTAGCGGGAGGCTAAAAAAGCAAAAATAAATAAATGAGACTACAACAAACT1710.11115878154374197No Hit
TGGCCTAGGGTGCGAAAGGTTTCCGTTTCAGAAATACAATGCCCTGATTTTA1710.11115878154374197No Hit
CATTCAGGGAGCACTTAGAACAATTTTTAGGACATAATAATTGTTCAGTAAA1710.11115878154374197No Hit
TGCGAGGGCTGCCGGTACCACCCTTTGGATTATTGTAGTGATATAGTAAGCT1700.11050873018968498No Hit
TTAAACGGGTGGACACATATGTGTACTGGTAAAAAGATCTCTGTACAGTACA1700.11050873018968498No Hit
TAACTAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1700.11050873018968498No Hit
CTACAAGGGAGGAGGGAAGATGGCGGACGAGGAGAAGCTGCCGCCCGGCTGG1700.11050873018968498No Hit
ATAGCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1700.11050873018968498No Hit
CTTGTCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1690.10985867883562801No Hit
CGTTGTGGGAAATGGATCTTTATTAGTTATTGGAAGAGGCGGTGTTACGGAG1690.10985867883562801No Hit
AACTTGGGGCGTTGAACTGGGGCAAGTACTCTGTAACACAAAGTGGGAAACC1690.10985867883562801No Hit
ATACCCGGGTATCTTAGTTTGCTACCAGCATCCAGCATGAAGTTGTAAAGTG1680.10920862748157105No Hit
ATTGGCGGGGAGAGTGATCTTAAATATGGCAAGATACAGCTCATTTAGAATA1680.10920862748157105No Hit
TGATACGGGATTATTATTTGCTAAGAAATTCTGTCAGGGCTTTGCCTTCATA1680.10920862748157105No Hit
GATATTGGGACCCGGAAGCAGGGGCCCGAGCGGAGCCGGCCGCGATGAGCGG1680.10920862748157105No Hit
GCGCACGGGGTTCTGAGTATACGGATTTATTTAGCTTAGAAGAGTAGGCTAA1670.10855857612751409No Hit
TCGCTTGGGTCGGAAGGACCACATGTTCACTCTGAGATCCCACTTTCATTTC1670.10855857612751409No Hit
GCCATAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1670.10855857612751409No Hit
AAATAAGGGTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAA1670.10855857612751409No Hit
ACCTAGGGATTACTGTGGACAGATTATTTGAGTTCTGTCATTTGAACATTTT1670.10855857612751409No Hit
GAAAACGGGATTTCTCTTAAACCAGGATTCTTTAAATGTTATTAACCAAGAG1670.10855857612751409No Hit
AGTATGGGGATGTCATACAATTTATTTGGCATGTCTTGTTTCAATACATCCT1670.10855857612751409No Hit
TACGAGGGGGATGGCGGGTTTCAGATGCATTATGAGAAGACCCCGTTTGATC1660.1079085247734571No Hit
GACGAGAGGGCTGCCAGATTTTTTTTTAACAAATTTATTAATCAATACATTT1660.1079085247734571No Hit
GTGGATGGGACAGCCGGGGAGGCGGGGTCCTTCTCTGGGGCGGTCGCGTTGG1640.10660842206534317No Hit
ACACCAGGGTTTCAATGTAGATCTTTAAGAGAAATCCTTTTCAAGAGTCGGA1640.10660842206534317No Hit
GGTGCCGGGAGGAGAGACAGTCTGTAAATATAACGAATGTGGAAGAACCTTC1640.10660842206534317No Hit
ATTTCCGGGAGGTTTATGTACATCATTATCATTAGAGGCACATAGAATCTAG1640.10660842206534317No Hit
CAGTGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1640.10660842206534317No Hit
CTGTGCGGGATCAATACAACTGATTTTCCTCCATCCGTTGTATTATTTAGCA1640.10660842206534317No Hit
GCATATGGGTGGCCCAGCCGCTCGCTCGGCTCCGCTCCCTGGCTCGGCTCCC1630.10595837071128619No Hit
AGTAGAGGGATGACTAATCACATATCCTGTTTCTGTAGTGACAAGTATTTAT1630.10595837071128619No Hit
CGCATGGGGAGGCTTAAGTCAGTTGTGGGGACCAACTCTCAGAGACTATTTC1630.10595837071128619No Hit
TCAAAAGGGCTAAGCAAGGGATGTTAATGTTCTCTGTTTATAAAAATTGAGT1630.10595837071128619No Hit
GAAGCCGGGATTGACAAACATCCACAAACATCAAGACCTTCCAGGAAAACGT1630.10595837071128619No Hit
ACGCAGGGGCAGAAATCCTCTGTGACGATCTGGATTTGAACCCGCTGACGTT1630.10595837071128619No Hit
ACTACTGGGGGGAAAATCGGTTCTTCCAAATTTTCTATATTCGATTATCAGG1620.10530831935722922No Hit
CTAGTGGGGCCACATACAGAAGAATGAAGTGGGACATTCACCTTATACCATA1620.10530831935722922No Hit
GTAGCGGGGAACTTCCTTCTTGCTGAACATATTTTATTCTCTTTTCAGAGAA1610.10465826800317224No Hit
ACTCGTGGGCAGTGGAACAGGAAATTGATTTAAAAGATGTTTTGAAGGCTCT1610.10465826800317224No Hit
AGGCTTGGGCTTCACTCGGGGCATTGAATTACTTTTCTTGTATAATGTTTTG1610.10465826800317224No Hit
CTCCTCGGGAACATACTGAAACTATTTATATTTTCTTTTTTAAGGATATTTA1600.10400821664911528No Hit
TGCATCGGGCAGCAAGGCCAAGGTGGAGTCTGAAGAGCCACAGGACAATGAC1600.10400821664911528No Hit
TGCTGACGGGTTACTACAGAAACTTGTCAAGGTTTTAATACTAAAAGTAAAG1600.10400821664911528No Hit
ACAAAGGGCAGCTTCCCAACAATACTTTGTGTCTTTTGTTCTCTCTTGGTCT1590.10335816529505831No Hit
TTCTATGGGCAGCAAATATACATGTCAAAACATTTGAGATGCAGCTAAAACA1590.10335816529505831No Hit
ACAGTGGGGGAACACAACTTCCGGCCCCACTGAGCGGTGTCCTGAGCCGATT1590.10335816529505831No Hit
GGCAATGGGCACTGTTTTAATTTGCATTTATTTGATTACAAATGAAGTTGAT1590.10335816529505831No Hit
TCACCCGGGGTTGGATTAAGTTCTAGCAGAAGACAGGTTGCCTCACTGAAGA1590.10335816529505831No Hit
TAACTAGGGGGGGGAAGGAGCAGCACCAAATCCAAGATGGCGGCCAGCAGGA1590.10335816529505831No Hit
TTTTAGGGATGTTCTTGGTAGCTAAGATCTCTTAAATTGCTTTGGAGAAAAA1590.10335816529505831No Hit
GTAGTGGGGGTACTGCATCACCAGTTTTTCTTACCTTCTGTTGACAGCTGAA1580.10270811394100135No Hit
TTGGAAGGGATCTGAGGCCAGTAACTATTAATATCTAGTTCTCAGAGCATTT1580.10270811394100135No Hit
TGTTAAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1580.10270811394100135No Hit
GCATTAGGGGACTACAGGTCTCTGAGAAATTATCTTTCAAAAGAGATTTCAT1570.10205806258694436No Hit
CATATAGGGGACAGCAGTGACAATCCAAAGTTTCACTTCTAGTCTTACCAGT1570.10205806258694436No Hit
ATCGACGGGTGAGGAAGTTGATCTTGAGTATGAAGTTTCATCTTAAACCAAT1570.10205806258694436No Hit
AACACAGGGCTAGTGAAGGGAAACCAAATCCTTCTCTGTATTTCCTGTTTTT1570.10205806258694436No Hit
GCTACTGGGATAATGGTAAAGGGATCAATGCAACAAGAAGAGCTAACTATCC1560.1014080112328874No Hit
CTGCTACCGGGCATACACTTGCTATATAGTAGTATGCAGTATTTATATTATT1560.1014080112328874No Hit
AAATGAGGGTTCCTGACCTCGTGATCCGTCCGCCTCGGCATCCCAAAGTGTT1560.1014080112328874No Hit
GTTCATGGGGATTATATGCTTTTTATTTACTACTTTTAGCCATTAACCAATT1560.1014080112328874No Hit
CGCTCTGGGGTTCCCGGAAGTTTTGCTGCTAGTCGCGGACGCAATGGCTTCA1550.10075795987883043No Hit
TCAGACGGGACCTTTACAGTTTGTTGAGAAACATACTTGTTGAATATTTCAG1550.10075795987883043No Hit
TTAAGTGGGCTTTTAAAACATTGATGAAAAACATTGAAGAAGAGACAAAGAA1550.10075795987883043No Hit
GAAAAAAGGGATAGATTATTATTGTACACAGGTAGTGTATTTTTATCCCCAT1550.10075795987883043No Hit
CGGCTTGGGGTGGGACTGAACTGATGAATAATTGAAATGATTTTTATATTTT1550.10075795987883043No Hit
TTTTGTGGGGTACAGAGATTATAAAGAAGAAATATAGCTCACAAAGGATGAG1550.10075795987883043No Hit
ATAGCCGGGGGCTCACTGCAACCTCTGGCGTCCGGGTTCAAGTGATTCTCTT1550.10075795987883043No Hit
ATTTTGGGGAGATAAATCAAATGAGCCATCAGTGAGGCAGCAGTTACTTCAA1550.10075795987883043No Hit
ATATTTGGGGAAAATTATTGCGTGCAACAGTATTTTCTCGTGTACCTCTTTT1550.10075795987883043No Hit
TGCCATGGGGAATGAGTGCTGCCCGCGCTGTGTCACAGACCCTTGCCAGGCT1550.10075795987883043No Hit
ATTCCTGGGACCCTGGAATTTCTTGTCTGAAGTGTTAACACTTTATTTCCAG1550.10075795987883043No Hit
CACATAGGGCACATGCAAGCATCCCCGTTCCAGTGAGTTCACCCTCTAAATC1550.10075795987883043No Hit
GTACAGGGGGAATTAGAATTAAAATGGTACATTATGAAATATTTATTTAACA1540.10010790852477347No Hit
AGTACAGGGTGTGTATCAAATAAAGTGAACAATTGGAGTTCTTTCTCTAATT1540.10010790852477347No Hit
GGCCACGGGATGCTTAGTAATGACTTCTGACACTCCTCAGCTTATCATTTGG1540.10010790852477347No Hit
TTAGATGGGTACATGATCAACGCAGTGACTCTTAACAACCTGCTCTTGCGGA1540.10010790852477347No Hit
CGGTTAGGGGCTCAACAAAATCACCTACAGTTATTAATGTCACTGTGTCAGG1540.10010790852477347No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)