FastQCFastQC Report
Sat 31 Dec 2022
SRR5846753.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846753.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151849
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGCCGTCT11860.7810390585384164TruSeq Adapter, Index 7 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGC11370.748770159829831No Hit
GTTCTCGGGCAACAGAAAATACATTCTTCTCAGCACCACATTGTATTTGTTC2320.1527833571508538No Hit
TGTAGAAGGGAAGTGACTTCTGATTTCTACTTTAAAAAATCACCAAGGGAAG2170.14290512285230722No Hit
CGTGCAGGGAGCCTTGGCGGAGGGAGAGTGCTATTTGCTTTTGTTCTCCCTC2150.14158802494583436No Hit
ACAAACGGGACTCCCCTTCAGCCCCCCGGCTGATGCCATCTCTGGTTCTGGA2150.14158802494583436No Hit
ACACGAGGGATCATGGGTGTTTTTAAGAACAAATCTAATTTTTATCAAGTGT2090.1376367312264157No Hit
ATGTGGGGATGGATATGTAGTAAAATTATGAAAATATGTATGAGGATAAGTA2090.1376367312264157No Hit
GCTATTGGGAATGCTGGACAGCTCCTTGACACAGGGCTGCTTTTCTTTGGTT2090.1376367312264157No Hit
GCGGCCGGGGGAGAAAAAAATACAAAACTAATCATAAAATTTTGCCTAAGGA2080.13697818227317926No Hit
GTGATTGGGCATTCTAAATGTCAAATCTGACAATTGGAAAACAGTACAGTTT2060.1356610843667064No Hit
TCTTTTGGGATACAAAAGCAAAGCATTTGATAACTTCAACAAAATTTTTCTG2060.1356610843667064No Hit
AATGACGGGGAAAGAATGAAGTTAAATTGTCTCTGTAGATGACATGATCTTA2010.13236833960052422No Hit
TGCAATGGGGAGTCTTCTGACCAAAAGACTTGAAGGAGGGCGATGTGTTTAT2010.13236833960052422No Hit
CAACAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1990.13105124169405133No Hit
TACCACCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1980.1303926927408149No Hit
GAAAACGGGATGAGTGAAAAGCGGGAATTTCCTACAGTGCCCTTGGTTAAAT1960.129075594834342No Hit
GTACAGGGGGAATTAGAATTAAAATGGTACATTATGAAATATTTATTTAACA1960.129075594834342No Hit
GTAGAGGGGGCTGCCTATAGTGTCTTTTGGAAAACTACTTAAAGGGTGATTT1950.12841704588110556No Hit
CAGAATGGGTGCCTGTAGCATGACCTGGAATTCTGTGTAATTACTGCTTTCA1930.1270999479746327No Hit
TGCTCTGGGTAAAACCCTGGAGGAGCTGTTCTGGTTCCTTTGAAAAATAGAC1920.12644139902139626No Hit
ACCTAGGGATTACTGTGGACAGATTATTTGAGTTCTGTCATTTGAACATTTT1910.1257828500681598No Hit
TGTTTGGGGACTGAACCACAAACCTAACCTCATTTTTTAAAAACTGAAGTGC1910.1257828500681598No Hit
AGCAAGGAAGGCCAACATTCAAATTCAGGAAATACAGAGAACACCACAAAGA1890.12446575216168694No Hit
GACAAAGGGTACTGTTCACTAAGGGAGGGAATATGTGGTTTATTTATTGAAT1890.12446575216168694No Hit
CGGCTTGGGGTGGGACTGAACTGATGAATAATTGAAATGATTTTTATATTTT1870.12314865425521405No Hit
TCACCCGGGGTTGGATTAAGTTCTAGCAGAAGACAGGTTGCCTCACTGAAGA1870.12314865425521405No Hit
CACATAGGGCACATGCAAGCATCCCCGTTCCAGTGAGTTCACCCTCTAAATC1870.12314865425521405No Hit
TGCGAGGGCTGCCGGTACCACCCTTTGGATTATTGTAGTGATATAGTAAGCT1860.12249010530197763No Hit
TAATACGGGACCTCTCTCTGCCTTCCAACCTGAAGACGCCCATCACGCTCTT1860.12249010530197763No Hit
ATTGCTGGGGTATCAAAACATCTCATGTACCCCACAAATACATACACCTACT1850.12183155634874118No Hit
GCTCAAGTCAAAAAACTCCGGAAGCAACAGCTTCCACCTTCAAGCAATCCAC1850.12183155634874118No Hit
GGTGCCGGGAGGAGAGACAGTCTGTAAATATAACGAATGTGGAAGAACCTTC1850.12183155634874118No Hit
GCAATAGGGAAGGCAGAGGCCTGCCCTGCTGCTGCTGGCGCTGTCACCTCCC1850.12183155634874118No Hit
TCTCAAGGGGCTGGACAGCAAACATGGAGAGAATCATGAAAGCTCAAGCCCT1840.12117300739550474No Hit
AACGCAGGGATTCTACAACATCATACTACACAATAATAAGACATATAGACTT1830.1205144584422683No Hit
ATTTGAGGGGGAGCATCTGCTTTTTGTCTTTATCTTTCAAAAGGTTTTTGCT1820.11985590948903188No Hit
TGTGAGGGGATCAGAGTCACAGGCTTTGACTGACCAATGCAGTTAGAAACTT1820.11985590948903188No Hit
GCAAAAGGGGGTTCTAGCCACTTGGTTCTCGTTTATTATTATAAAACTTCAA1810.11919736053579544No Hit
GATATTGGGACCCGGAAGCAGGGGCCCGAGCGGAGCCGGCCGCGATGAGCGG1810.11919736053579544No Hit
ACATGCGGGGAGCGGCGGGGTCGACTGACGGTAACGGGGCAGAGAGGCTGTT1800.118538811582559No Hit
TCAAAAGGGCTAAGCAAGGGATGTTAATGTTCTCTGTTTATAAAAATTGAGT1770.11656316472284968No Hit
ATACATGGGCAATCTTTCTAATGGTGGAACTTTTTAATATAAAGTTTTTATT1770.11656316472284968No Hit
TCAGCAGGGGATGAAAGTGTCACGGGTAAAAACTCTAAAAGGTTAATTTCTG1760.11590461576961324No Hit
AAAGGTGGGCATCCTCACATGTGGCACGTAAGGTCTCATTTGATATTGTGTA1760.11590461576961324No Hit
GTAATTGGGTGCGCAGAGCGGTTTGGTCGTTCGTTGGGCGGTGCTGGTTTTT1760.11590461576961324No Hit
AAGGCCGTGGAGGAGCTAAGGACACAGGTCCGCGAGCTGAGGAGCATCATCG1760.11590461576961324No Hit
TAAGTGGGGAGTCTCAGAATACAAAATCAATGTGCAAAAATCACAAGCATTC1760.11590461576961324No Hit
ACAAAGGGCAGCTTCCCAACAATACTTTGTGTCTTTTGTTCTCTCTTGGTCT1750.1152460668163768No Hit
AGGCTTGGGCTTCACTCGGGGCATTGAATTACTTTTCTTGTATAATGTTTTG1750.1152460668163768No Hit
GCACCTGGCTGTCTGATTCCATTTTAAAAAGAAAAGAAAACTCTTTGTTTTG1730.11392896890990392No Hit
GTAGGCGGGACAATTTTGATTTTATATTGCTTGTTATAATATTAAATAACAG1730.11392896890990392No Hit
CACGATGGGAGCCAGATCAGTTGATTAAGTGTCCCCGCCGGAACCAGCCAGT1720.11327041995666748No Hit
TTAGATGGGTACATGATCAACGCAGTGACTCTTAACAACCTGCTCTTGCGGA1710.11261187100343104No Hit
CTGTGCGGGATCAATACAACTGATTTTCCTCCATCCGTTGTATTATTTAGCA1710.11261187100343104No Hit
GCCATAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1700.11195332205019459No Hit
GTGCATGGGCAGGTTTACAAACTCAGACCCTTTTAAAAGTTGTTTAAATTGT1700.11195332205019459No Hit
TTTGGCGGGTCTACTGAAGGGGAGCCCCGAACAGAGTGAAGAGTCTTTGGTG1690.11129477309695818No Hit
ACTACTGGGGGGAAAATCGGTTCTTCCAAATTTTCTATATTCGATTATCAGG1690.11129477309695818No Hit
TGACTGGGGATTGTGAACTCTAGCTTTGATCATGGTAAAAAGTTAACCTTTT1680.11063622414372173No Hit
CCAGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1670.10997767519048529No Hit
AGCATAGGGGTGGGGAAGATATTCAGTTTATAATAGATGAGATTACACTAAT1670.10997767519048529No Hit
AAGGTAGGGCCAGTGGGCCGATCTGATCATCTGCAAGACCCGGAGGAACTCG1670.10997767519048529No Hit
ATCTCGGGGGTTGTCACAGAGGCAAGTGCTACCGCACAGGCATGCTGCAGTG1670.10997767519048529No Hit
TCAAAGAGGGGTTTTTACGGCGCCGTAAAGCAGCCCGGCCGAGCAGACTGCT1660.10931912623724883No Hit
CTGCATGGGATTCACCAAAAATGGATTTCAAAATGACAGTAGTTCAGCTACA1660.10931912623724883No Hit
AACTGTGGGCATCCTCATCGACATGGTCCTGAACCGCATGGCAAAAGGAGTG1660.10931912623724883No Hit
CCAAGCTGGAGAGAATTTCTTTGCTTCAAATACATATGCAGTGTTAATCTAT1660.10931912623724883No Hit
AATGCCGGGAGGCAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGG1660.10931912623724883No Hit
AACATCGGGATATCAACACACCCAGTTAATTTTTTTTGTTTGTTTATTTTTG1650.10866057728401239No Hit
ACTCGGGGGGCTGACACAGGAGAATCGCTTGAACACGGGAGGCGGAGATTTC1650.10866057728401239No Hit
AAATGAGGGTTCCTGACCTCGTGATCCGTCCGCCTCGGCATCCCAAAGTGTT1650.10866057728401239No Hit
ACTTGAGGGGCCTCTGTCATGGGCAGCATATTTAACAGAATAAATTCTAAGC1640.10800202833077598No Hit
CTTGTCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1640.10800202833077598No Hit
GATATGGGACCTATATTTACTCATTAAACAAAATTTTAAAAATAAGACAAGA1640.10800202833077598No Hit
TCATGCAGGGGGTTTTAGAAAATGTAGTTTACGTAATTGCAGCTTGACTTTT1630.10734347937753953No Hit
AGTCTCGGGCTATTCTATTATATATAATAACTGCCTATTAATTAAATGGGAA1630.10734347937753953No Hit
ATGGGGAGGGCAAGGAAGGAAAGATCTGCACGTGTAAAACATGGGGCAGCCT1630.10734347937753953No Hit
CAGTGCGGGAAATACTTACCATGTGACTTTTTATTACCAAATGCATCAGTAG1630.10734347937753953No Hit
CCACAAGGGTACCAACACAAGATTAAAGGTTTAAAAATCTCTTACGTGTACA1630.10734347937753953No Hit
TATGCTGGGACGTTCACACTATTTGGTTCCAAACTATTTTTTTTTAATTTGG1620.10668493042430309No Hit
TGCATCGGGCAGCAAGGCCAAGGTGGAGTCTGAAGAGCCACAGGACAATGAC1620.10668493042430309No Hit
AATTTAGGGAACTACTCAGTGCTGAAGCAGTGTCAGAATTTATAGGCCTCTT1620.10668493042430309No Hit
CCTGAAGGAAATCAAAGCTTTTTTAATCCCAAAATACATTCCTTTTACATAT1620.10668493042430309No Hit
AGTACAAGGGCATGTAAATTAACTTTGGTAACTTGTGTCATCTTCAATACAA1610.10602638147106665No Hit
AGTAGAGGGATGACTAATCACATATCCTGTTTCTGTAGTGACAAGTATTTAT1610.10602638147106665No Hit
GTAGATGGGGAGGAAGCTTGGAGTGCTTCATTTTCAGGAAGGCTCGGGCTGT1610.10602638147106665No Hit
AGCCAAGGGTATTCCACTGATCAACCAAGACTGGTTCTGGTTGGACAGCTAA1610.10602638147106665No Hit
GTTCATGGGAAGTTGCAAGATCTTATTTATTAACTAGGTATGAAGTATAAAC1610.10602638147106665No Hit
CTCCTCGGGAACATACTGAAACTATTTATATTTTCTTTTTTAAGGATATTTA1600.10536783251783022No Hit
GCATAAGGGGCTTCGTAAGGATGATGCCGAAAGGGAACTGGGGTCTCCATGG1600.10536783251783022No Hit
TGGCCTAGGGTGCGAAAGGTTTCCGTTTCAGAAATACAATGCCCTGATTTTA1600.10536783251783022No Hit
TAATATGGGGTTCTATATAGGAAATGATACTAACCTAAGGTGGCTTTTGTGT1600.10536783251783022No Hit
GTAGCGGGGAACTTCCTTCTTGCTGAACATATTTTATTCTCTTTTCAGAGAA1590.10470928356459377No Hit
ATTTTGGGGAGATAAATCAAATGAGCCATCAGTGAGGCAGCAGTTACTTCAA1590.10470928356459377No Hit
CACAAAGGGACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAGC1590.10470928356459377No Hit
GTCACTGGGCATAGAACTTATAAAGGTCCCAGGATCACTTTTAAGGGATTTT1590.10470928356459377No Hit
GAAAACGGGATTTCTCTTAAACCAGGATTCTTTAAATGTTATTAACCAAGAG1590.10470928356459377No Hit
CTAGTGGGGCCACATACAGAAGAATGAAGTGGGACATTCACCTTATACCATA1590.10470928356459377No Hit
GCATATGGGTGGCCCAGCCGCTCGCTCGGCTCCGCTCCCTGGCTCGGCTCCC1580.10405073461135733No Hit
TGATACGGGATTATTATTTGCTAAGAAATTCTGTCAGGGCTTTGCCTTCATA1580.10405073461135733No Hit
TTTTAGGGATGTTCTTGGTAGCTAAGATCTCTTAAATTGCTTTGGAGAAAAA1580.10405073461135733No Hit
GACGAGAGGGCTGCCAGATTTTTTTTTAACAAATTTATTAATCAATACATTT1580.10405073461135733No Hit
GTGGATGGGACAGCCGGGGAGGCGGGGTCCTTCTCTGGGGCGGTCGCGTTGG1570.10339218565812089No Hit
GTTAGCGGGAGGCTAAAAAAGCAAAAATAAATAAATGAGACTACAACAAACT1570.10339218565812089No Hit
TCAGAACGGGACAGTGTGTGCGGTGTGATTATTGATGTCAAGCGCAGAATGC1570.10339218565812089No Hit
AACTTGGGGCGTTGAACTGGGGCAAGTACTCTGTAACACAAAGTGGGAAACC1570.10339218565812089No Hit
TCTAGCGGGTGAATTTATCTGCAGCTTAAATTCAAGTGAAACTTCATTCTCA1560.10273363670488447No Hit
GTTCATGGGGATTATATGCTTTTTATTTACTACTTTTAGCCATTAACCAATT1560.10273363670488447No Hit
TCTACCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1560.10273363670488447No Hit
TGTTAAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1560.10273363670488447No Hit
GCGTACCGGGAGATTTGAAGCGCTTCAAAGGACCGGACCCAGAGAAGAGGAA1560.10273363670488447No Hit
GTTGTGGGGAAGAAGTATTTGGGTGATGAGGAAAATTAAGCCGATAGCATTT1550.10207508775164802No Hit
TGTTAAGGGATTTCAAGGAATGCTAGTTAATTTCCCCTTTCCCACTTCCCAC1550.10207508775164802No Hit
AGCGCGGGGTATTCAAAGCCTTTTTAAAGGAACCAACTACTCAAATGCTCTA1540.10141653879841157No Hit
GCATAGGGGTACTGAAGGAGTCACCAGTGATGAGGGAGGGAACAGTCTGAAA1540.10141653879841157No Hit
AAGCATGGGATATTCTTGAATGAAAAAGATCCACATTTCATCATTTGCCTGT1540.10141653879841157No Hit
CTAGGCGGGGACACCTCATTAGGTTATTTACAGTTTTTCTACTTTTTTGGTA1540.10141653879841157No Hit
ATTACAGGGATGTTCAGTTCGAGCGCCAAGATCGTGAAGCCCAATGGCGAGA1540.10141653879841157No Hit
CTTCACGGGGCTGAAAACAATAATCAAATATGAACATAAGAGATAACAGGTA1540.10141653879841157No Hit
TATCGCGGGAAACTTTGTTACTGAAGAAGACAAGAGGATTCTTCGTGACATT1540.10141653879841157No Hit
CGACATGGGCACCCTCCAAGTAATCTTTGAGGTGGCCAGTGCTGGCAGGCGC1530.10075798984517513No Hit
AATCATGGGGAGTTCATATTTAAAGTGATCCAGGCCAATGCATGGCTATTGC1530.10075798984517513No Hit
CATTCAGGGAGCACTTAGAACAATTTTTAGGACATAATAATTGTTCAGTAAA1530.10075798984517513No Hit
AACAATGGGAGACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTT1520.10009944089193872No Hit
GAAAAAAGGGATAGATTATTATTGTACACAGGTAGTGTATTTTTATCCCCAT1520.10009944089193872No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)