FastQCFastQC Report
Sat 31 Dec 2022
SRR5846742.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846742.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130861
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGCCGTCT10060.7687546327782915TruSeq Adapter, Index 7 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGC9280.7091494028014458No Hit
TAGGCTGGAAGGCATTGTGTTAATTATTATATGTGTACTCTGACAAAAATTC1450.11080459418772591No Hit
AATGATGGGTAGCATTTCAAAAATATATATAAAGTTGTTCTTTTTCCTATTT1450.11080459418772591No Hit
GGCATTTTAAAAGTATTTTGAAGCAATTTCCAGACATCATAATATCATATTT1410.10774791572737484No Hit
TGACGCGGACAAAGAGGTTTGCTATATTTAAATTCTTTTTGGAATTTGCAAA1410.10774791572737484No Hit
CTTGGTGGGGGGTCAAAAGAATCTGTTCCAGAATTCCCCCTTTCCCCTCCAA1390.10621957649719932No Hit
CTCTTAGGGGTCAGGCCCGGCCACTGCCCTGTGGGTGTTATCTCAGGCCTGC1320.10087038919158496No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)