FastQCFastQC Report
Sat 31 Dec 2022
SRR5846741.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846741.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129375
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGCCGTCT10050.7768115942028985TruSeq Adapter, Index 7 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGC8510.6577777777777778No Hit
CACGATGGGATTGTTTAATAAATCATTAAATTATTTCATTAAAAAAGAAAAA1370.10589371980676329No Hit
CTCTTAGGGGTCAGGCCCGGCCACTGCCCTGTGGGTGTTATCTCAGGCCTGC1370.10589371980676329No Hit
AATGATGGGTAGCATTTCAAAAATATATATAAAGTTGTTCTTTTTCCTATTT1370.10589371980676329No Hit
TAGGCTGGAAGGCATTGTGTTAATTATTATATGTGTACTCTGACAAAAATTC1330.10280193236714977No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)