FastQCFastQC Report
Sat 31 Dec 2022
SRR5846740.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846740.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163658
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGCCGTCT12790.7815077784159651TruSeq Adapter, Index 7 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGC12280.7503452321304183No Hit
AGTGCTGGGATTGTGACTTAGTTAATATAAGTTGCTTTTTCAAAAGGAGAAA2150.13137151865475566No Hit
AATCGCGGGTAAGAGAAAATATGAAGACTTAGAAGAGTAGCATGAGGAAGGA2040.12465018514218676No Hit
CCTCTTGGGGGGCCTTGGCGCGCCCCGGCAGGTCGCCGTCCGCCGCCTGGGC1930.11792885162961787No Hit
TGGTGCAGGAAATAAAAAAATCTGAGTAGTATATTTTGCCATAAATCATAAA1870.11426266971367119No Hit
TACTTAGGGTCACTAGCTCTTTGTTATTTGTCTGTTAGGTCAGGAACGATGT1870.11426266971367119No Hit
GCTTATGGGAACCCACATAGTTGCAAAACAGACAGGTTTTCATGAATCAATT1800.10998545747840008No Hit
TCTGCCGGGTGTGCAGATTATTCATAATATAGAAGTTAAAATAAGTATTAGT1780.10876339683975118No Hit
ACAGATGGGATAGTTTGAAGTCAGGGAATGTTATGTCTCCAGATTTGTTCTT1760.1075413362011023No Hit
GCCGTTGGGATGGACCTGGCTAAATAGCAGATGAAGGAATTAGTAACTAGCA1750.10693030588177785No Hit
TAAGCCGGGACTACTGAATTAGCAGAAATAACGATATCTAAAGCTTACCAGC1740.1063192755624534No Hit
TTCCTAGGGGGGAAAAAAGAGAAAAAGGAGAAAAAAGGTGTGTGTGTGGGGT1720.10509721492380451No Hit
GAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCGGGA1720.10509721492380451No Hit
ACATATGGGCATGGGATTTGGTCTGTACCTGATGGATGGGAGTGTCAGTAAC1710.10448618460448009No Hit
TACGCTGGGAAGGCTCAGTTGCATCCTTGTTTTTACAGCACTGCAGTGAACT1660.10143103300785784No Hit
GCCCAGAGGAAACAATATGAAATATGGGGGAGATGATTGACAATTTAATCTA1640.10020897236920896No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)