FastQCFastQC Report
Sat 31 Dec 2022
SRR5846739.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846739.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164327
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGCCGTCT12680.7716321724366659TruSeq Adapter, Index 7 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGC12060.7339025236266712No Hit
AGTGCTGGGATTGTGACTTAGTTAATATAAGTTGCTTTTTCAAAAGGAGAAA2190.13327085628046517No Hit
TGGTGCAGGAAATAAAAAAATCTGAGTAGTATATTTTGCCATAAATCATAAA2120.1290110572212722No Hit
TACTTAGGGTCACTAGCTCTTTGTTATTTGTCTGTTAGGTCAGGAACGATGT2120.1290110572212722No Hit
GCTTATGGGAACCCACATAGTTGCAAAACAGACAGGTTTTCATGAATCAATT1900.11562311732095153No Hit
GAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCGGGA1830.11136331826175858No Hit
GTAAAGGGGAGGTGCCACTGAACTGAAGGGGTGAACTAGAGGAGAAGAGGGT1810.11014623281627486No Hit
CCTCTTGGGGGGCCTTGGCGCGCCCCGGCAGGTCGCCGTCCGCCGCCTGGGC1810.11014623281627486No Hit
ACTGTTGGGCCGGCAAGGGGAGTTTGTTGTTGCCATGGGAAGTCCCTTTGCA1810.11014623281627486No Hit
AATCGCGGGTAAGAGAAAATATGAAGACTTAGAAGAGTAGCATGAGGAAGGA1800.10953769009353301No Hit
CTCATAGGGCAACAAATTTAGAAATTTCAAATGCTATATTTTATGAAATTGA1730.10527789103434006No Hit
GCAGTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG1730.10527789103434006No Hit
GATGGTGGGGTAAATTAATACTTCATTTGGTGCTGGTCTATTTTGATTTTGG1690.10284372014337266No Hit
CTGCAAGGGAACTGGAAAACAACTAAGATGTAGTAATTTTTTTTTCCTGGTT1680.10223517742063082No Hit
TCTGCGGGATATAAAGGGATAAACATTGAAGTTATTGGTCTCTTCCTTCTCA1670.10162663469788896No Hit
TCGCTCGGGGTGTACCCAACCGTGGAATTTGCCTCCCAAACTATAGCAAAAC1670.10162663469788896No Hit
GCCGTTGGGATGGACCTGGCTAAATAGCAGATGAAGGAATTAGTAACTAGCA1670.10162663469788896No Hit
TTCCTAGGGGGGAAAAAAGAGAAAAAGGAGAAAAAAGGTGTGTGTGTGGGGT1670.10162663469788896No Hit
ACATATGGGCATGGGATTTGGTCTGTACCTGATGGATGGGAGTGTCAGTAAC1660.10101809197514712No Hit
CTATTTGGGCATTCTTCATAACAAAGACCTAACCTCCAGGGGAAAAATTTTA1650.10040954925240525No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)