FastQCFastQC Report
Sat 31 Dec 2022
SRR5846738.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846738.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163018
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGCCGTCT12690.7784416444809775TruSeq Adapter, Index 7 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGC10950.671704965095879No Hit
AGTGCTGGGATTGTGACTTAGTTAATATAAGTTGCTTTTTCAAAAGGAGAAA2040.12513955514115008No Hit
TGGTGCAGGAAATAAAAAAATCTGAGTAGTATATTTTGCCATAAATCATAAA1980.12145897998993976No Hit
GCACTTGGGAGACAGTTCCAACTGGTAAATTCTCTGGTGCTCGATGCCTCAG1940.11900526322246623No Hit
TACTTAGGGTCACTAGCTCTTTGTTATTTGTCTGTTAGGTCAGGAACGATGT1920.11777840483872946No Hit
AGCTATGGGACTTTCTCACTGTAATGTGTTCCTTCATTGCCTGTGAGAAGGA1840.1128709713037824No Hit
GAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCGGGA1810.11103068372817725No Hit
TAAGCCGGGACTACTGAATTAGCAGAAATAACGATATCTAAAGCTTACCAGC1770.10857696696070372No Hit
CCTCTTGGGGGGCCTTGGCGCGCCCCGGCAGGTCGCCGTCCGCCGCCTGGGC1720.10550982100136182No Hit
ACATATGGGCATGGGATTTGGTCTGTACCTGATGGATGGGAGTGTCAGTAAC1720.10550982100136182No Hit
TACACAGGGCAGTGAATTAACTCTGCCTTTTTTTCCCATTTGAGCTCATAAA1700.10428296261762506No Hit
GTAAAGGGGAGGTGCCACTGAACTGAAGGGGTGAACTAGAGGAGAAGAGGGT1700.10428296261762506No Hit
GCTTATGGGAACCCACATAGTTGCAAAACAGACAGGTTTTCATGAATCAATT1680.10305610423388828No Hit
TGACCAGTAGCTTAAACTTTTTTCAGGATTTTAGGTAATTTGAAAGGAGTTT1660.10182924585015153No Hit
TGTACAGGGATACAATATGCATTGCATCTGGACCTTAAACTTATAAAAATGG1660.10182924585015153No Hit
GCCGAGGGATTGCATAAATTATCAATTCTAAAAATCTGTCATTCCAAGGGCA1660.10182924585015153No Hit
GAAGTTGGGTGAACCAAGACATCTGTTGGTTGACCTTCTCAGAAAGAGACCA1640.10060238746641476No Hit
TTCCTAGGGGGGAAAAAAGAGAAAAAGGAGAAAAAAGGTGTGTGTGTGGGGT1640.10060238746641476No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)