FastQCFastQC Report
Sat 31 Dec 2022
SRR5846737.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846737.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177154
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGCCGTCT12580.7101166216963771TruSeq Adapter, Index 7 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGC10310.5819795206430564No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTTCTCGGGCCTGAGTTC2970.1676507445499396Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCTTGGGGGATATGTGACATAAGTGAGGTGAGTCATGGCTAGCATTCATT2900.16369938020027774No Hit
GACTTGGGGTCTGATATCCAGGTGAAAGAACTGGAGAAGCGTGCCTCAGGCC2630.14845840342301048No Hit
CATCAAGGGGTGAAAATAGAAGTTTGGCTGCTAAATAAAAGAAAATTATCTC2380.13434638788850378No Hit
GCTCTTGGGGAAATATAACTTTTGTCAAAGAAATACTCTCAGAAGAGAAATG2340.13208846540298272No Hit
TTAGTTGGGGATGGGGCTGTGGCATCCAAATCCACAAAATGGAAATAACTAT2290.12926606229608137No Hit
ACTCGGGGCTGCCTGTTGTACCGTGGTGACGTGGTTCCCAAAGATGTCAATG2220.1253146979464195No Hit
TCGTTAGGGTTAGCAGCCGTGGCAACTGCCAAGGACACATACAACAAAAAAA2200.12418573670365896No Hit
TAAGTTGGGGAATCAACCCATAGAAGTGACAGCGCTGTATTCATTTGAAGGA2200.12418573670365896No Hit
CGTACTGGGATATTTTATTATTTGAATCTAAATTAGTAATTTCAAATCTGTC2170.12249229483951816No Hit
GGCATCGGGAGTCTTTGCAAAGAATGAAGAAGTGAATTCGCTGTACATTTGT2130.12023437235399709No Hit
GTTCGGGGAGTCTCTTCAGTAAATGATGTTGGAAAAACTTGGTATCCACATG2070.11684748862571548No Hit
AAAGACGGGACGTGCTAAGACCCGAGTATCGTGTTTAATTGCAGTGTAGGCA2060.1162830080043352No Hit
AACATGGGGTGAGGGCTGGCTATTGAGAACTGGAAAGAGTAAAATGATAACT2050.11571852738295493No Hit
TAGGCTGGGGCAGGAAATCAAAGAGAGAGACCCAGAAGTCAGACTCCAAATT2040.11515404676157469No Hit
CGATGAGGGGGCCAAAAACAAAATTGTAAAGGAAATTTAAATTCTGGAGAAT2030.11458956614019442No Hit
TTCGCCGGGAGCTAAACAGTACTTGCATACAATGGACACTAAATTTATATTT2030.11458956614019442No Hit
CGTTCCGGGTGCTGCATATCGAGCTAAAGGAAAGCCTGATGCAGCAAAAAAG2030.11458956614019442No Hit
GCATCGGGGACTAGCATACGTTTTTCTTAATTGTTATCAGCATTGACCAAGT2030.11458956614019442No Hit
CCGTCAGGGACCAGTAAGCTGGACTGCTGAGAAGAATAAATAACAGTTCCTA2020.11402508551881414No Hit
GGCATGGGGTTCCTTGACATTGTTCCATTAACTTGTGTTCTGAAACTTCTGT2020.11402508551881414No Hit
TTTGCAGGGTGGTATCTCACTGTGGTTTTGATTTACATTTCTCTAATGACCA2000.1128961242760536No Hit
CTAGATGGGTGTTTCCATTTGAAAGGAACTGTAAGCTTTTATCTTTTAACCA2000.1128961242760536No Hit
AAAACTGGGTGGAGGCTGGAGGTAGCTGATATAAGATTTATGTTTAACAGAA1980.11176716303329307No Hit
AACACAGGGGAACTAATGGTAAGACACCAAACCATATCCAGGAAGCTAAAAG1980.11176716303329307No Hit
CCCCCTGGGCACAGACACCACTGCCCCATTGAGCACATATCTGATGAAATTA1960.11063820179053253No Hit
TGCTGTGGGCAGATGAAGGAATGTTTTATAGTAATTTAATTTGCTCCTGTAG1960.11063820179053253No Hit
TCATATGGGTCAGAAAAAAAAGTTTAAGTCAGAAAGCTTGTCAGCTCATTGG1960.11063820179053253No Hit
TCTGTTGGGTGTATCCTAGCTGACAAATTATTGATTAATAAGAACTTGAATT1940.109509240547772No Hit
GTACTAGGGATACTTTAAAACAACACGAACAACTGGAGTTGACTCAAAAAGA1940.109509240547772No Hit
GCAGGGGATCTCTCTTTACTGCCAGTGATTACAAAGTGGCTTCTGCTGAGTA1920.10838027930501146No Hit
AAAATTGGGACTTGGAATAATTTGTTTACTCAAATCTGTAAAGTTTTATATA1920.10838027930501146No Hit
ACATCCGGGATATCCTATGTCTTTTACCTTCCTGCTTTGCTCATTTAACTGC1900.10725131806225094No Hit
ACAAAGGGGGTTTAGATATAAGATTTCTTATCTTTAAGGATTTGTAAAACAA1900.10725131806225094No Hit
GCAACATGGGTGTTTGAAAAATTTTTAATGCTGACTGAAGCTTGCCATTTGA1900.10725131806225094No Hit
TGTAAAGGGGAGATAGTCTTATAAGAATCACGATTTTCTACACCTGTCATTG1890.10668683744087067No Hit
TCATATGGGTGATGCCCAGCAGGTAAAATGGGCTTAATGCTCTTTTGTTTTT1890.10668683744087067No Hit
TGCACAGGGCTTCCTTTAATATTTCTTGAAAGGCAGATTTACTGGCAACACA1870.10555787619811012No Hit
TTAGGTCAGCTGCTTAGCAACCGCAATCCATCTGCAAACTTAATTTACACAT1870.10555787619811012No Hit
TCCTTAGGGCAATTCAGAAGAATAAAACCCCTTTGCGTGACACGATATGGGG1870.10555787619811012No Hit
AGAAACGGGTATCAGATTCTTCTCTTTGAAGAACACGTTGATTAGACGTTCT1870.10555787619811012No Hit
ATTGCCGGGCAGAGACCTGAGTATGTTTGGGTTTAGGAGACACTGAGTTACA1860.10499339557672985No Hit
TGTTACGGGGCACATGAATTTAATTTTTTGAAAATTTATTTAAGTAGTCTAG1860.10499339557672985No Hit
TGTTGCGGGATATAATAAACAGTAAACGTTGAGGTTCATCAATAAAAGCACA1850.10442891495534957No Hit
CATATTGGGAACAGAGAGAGCTTGGAAAAGAGAATTCAGACCTACCTTCAGT1850.10442891495534957No Hit
AAGTACGGGAGAAAATCATCAGACAAATCCAGATTAAGGGATATTTTGCAAA1840.1038644343339693No Hit
GAAACAGGGGGGAGTTTCGCCGCCGCAGTCTTCGCCACCATGCCGCCCTACA1840.1038644343339693No Hit
TAGCCGGGACTCTCCAGGCTCTCCATCTTGATTTATGCTTGAGTTGTTATGT1830.10329995371258904No Hit
TAAACTGGGGATGAAATACAAATTACAATTGTATTAACTTCACCAAACATGC1830.10329995371258904No Hit
ACTTCAGGGCCTCAGTTTATAAAATTCTATAATTCTAAACACAGCAGTAATG1830.10329995371258904No Hit
TCAATAGGGGAGATAATTTATGCTTATTGGAGTCCTAGTATAGAGGTGGTGC1820.10273547309120878No Hit
TCTCACGGGAAAGATAAACCATTCAAAACCAGTTTTAAGTATGATCTGTAAA1810.10217099246982851No Hit
TCTCTCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1810.10217099246982851No Hit
GTAGGCGGGATTATAAAAGGCCATTTCCCAAATCTAGAATTTATTACTAAAA1810.10217099246982851No Hit
GTCTCTGGGTGCTAAATGAGTATTACAACTGGCTAATATCATTTTTTATATA1810.10217099246982851No Hit
CTTACAGGGTCTGATGTTATCTGTTCAAATATTCCACATTGTGGAAAATCCT1810.10217099246982851No Hit
GAAATAGGGGCCTTTCTCGTTCCCCGGCCATCTTAGCGGCTGCTGTTGGTTG1810.10217099246982851No Hit
TGATAGGGGTCAGAGAGTGTTTCAGTATTATTCTGTAAAAGCATCCAGGGAT1800.10160651184844824No Hit
GTTACAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1800.10160651184844824No Hit
TTTTGTGGGGTACACCTGACCTACAGTGTTCCTATAATGAAAAGTGGTTGCA1800.10160651184844824No Hit
AGTCATGGGACGTGACGAGGAACTAGCCTTAGCGAGGCCATGGGGGAAAAAG1790.10104203122706798No Hit
CATTGTGGGACGCGCAGGAAACATGGCGGCGGCGGGTGTTGTGAGCGGGAAG1790.10104203122706798No Hit
CTAGGTCCGGGAACATGAAAATTGTTTAGTTTTTCTTGGTTTCATAAACATT1790.10104203122706798No Hit
GTACAGGGAGTCTAAGATTAAGAGTACCATTCTGAACGGTGTAGTATTGAAA1780.10047755060568771No Hit
GAACAAGGGTAAATAAAGATGGGACCCCGAGAGAAGGGACTAGGACTAAACG1780.10047755060568771No Hit
ACCAAAGGGATAGTTAATTCTCATTAACACTTACATTTCCATAAAGAAAACT1780.10047755060568771No Hit
GTGTTTGGGAGTGAGACCCTATTTCAAAAAAGAAAAGAAAAGAAATTGAAGA1780.10047755060568771No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)