FastQCFastQC Report
Sat 31 Dec 2022
SRR5846736.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846736.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177777
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGCCGTCT11880.6682529236065408TruSeq Adapter, Index 7 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGC10290.5788150323157664No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTTCTCGGGCCTGAGTTC2880.1620007087531008Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GACTTGGGGTCTGATATCCAGGTGAAAGAACTGGAGAAGCGTGCCTCAGGCC2530.14231312261991147No Hit
TAAGTTGGGGAATCAACCCATAGAAGTGACAGCGCTGTATTCATTTGAAGGA2500.14062561523706668No Hit
AAGCTTGGGGGATATGTGACATAAGTGAGGTGAGTCATGGCTAGCATTCATT2410.13556309308853226No Hit
GCTCTTGGGGAAATATAACTTTTGTCAAAGAAATACTCTCAGAAGAGAAATG2390.13443808816663574No Hit
TTAGTTGGGGATGGGGCTGTGGCATCCAAATCCACAAAATGGAAATAACTAT2370.1333130832447392No Hit
TTTGCAGGGTGGTATCTCACTGTGGTTTTGATTTACATTTCTCTAATGACCA2300.12937556601810132No Hit
CGATGAGGGGGCCAAAAACAAAATTGTAAAGGAAATTTAAATTCTGGAGAAT2280.12825056109620478No Hit
GCAACATGGGTGTTTGAAAAATTTTTAATGCTGACTGAAGCTTGCCATTTGA2260.12712555617430826No Hit
CTAGATGGGTGTTTCCATTTGAAAGGAACTGTAAGCTTTTATCTTTTAACCA2240.12600055125241175No Hit
GTTCGGGGAGTCTCTTCAGTAAATGATGTTGGAAAAACTTGGTATCCACATG2200.12375054140861866No Hit
ACTTCAGGGCCTCAGTTTATAAAATTCTATAATTCTAAACACAGCAGTAATG2180.12262553648672214No Hit
TGGTCAGGGGTATCCTATGTTATTTGTTTGACTTAGTTTATGATCCCATTTG2140.12037552664292907No Hit
ATCAGAGGGCTTCCAGAGGGGTTGCCAAATAAATACACTGGGGAAAAAGACA2130.11981302418198081No Hit
ACATCCGGGATATCCTATGTCTTTTACCTTCCTGCTTTGCTCATTTAACTGC2110.11868801926008425No Hit
TCCTTAGGGCAATTCAGAAGAATAAAACCCCTTTGCGTGACACGATATGGGG2110.11868801926008425No Hit
GTACTAGGGATACTTTAAAACAACACGAACAACTGGAGTTGACTCAAAAAGA2090.11756301433818774No Hit
TGTTGCGGGATATAATAAACAGTAAACGTTGAGGTTCATCAATAAAAGCACA2090.11756301433818774No Hit
GGCATCGGGAGTCTTTGCAAAGAATGAAGAAGTGAATTCGCTGTACATTTGT2080.11700051187723948No Hit
CATTGTGGGACGCGCAGGAAACATGGCGGCGGCGGGTGTTGTGAGCGGGAAG2080.11700051187723948No Hit
GTCTCTGGGTGCTAAATGAGTATTACAACTGGCTAATATCATTTTTTATATA2080.11700051187723948No Hit
CGTTACGGGTCTACATCAGGGATGCACACACACATTCAGTATTTTAAAAGAA2060.11587550695534292No Hit
TCGTTAGGGTTAGCAGCCGTGGCAACTGCCAAGGACACATACAACAAAAAAA2060.11587550695534292No Hit
AGTCATGGGACGTGACGAGGAACTAGCCTTAGCGAGGCCATGGGGGAAAAAG2060.11587550695534292No Hit
GTGTTTGGGAGTGAGACCCTATTTCAAAAAAGAAAAGAAAAGAAATTGAAGA2020.11362549711154987No Hit
AAATTGGGGTAGAAACCGGAGCCAGTCAAGATTGGCTGCCTCATGTTGAGGT2000.11250049218965333No Hit
TAGCTGGGGTGGATTTCCTTTGTTGTGTTCACAAGGAGATCTGTCAGCTGCT1990.11193798972870507No Hit
AACACAGGGGAACTAATGGTAAGACACCAAACCATATCCAGGAAGCTAAAAG1990.11193798972870507No Hit
CATCAAGGGGTGAAAATAGAAGTTTGGCTGCTAAATAAAAGAAAATTATCTC1980.1113754872677568No Hit
TGTACTGGGCATGCCATAATCTTGCAGTTTTCTGGGACATCTAGGTACTTTG1980.1113754872677568No Hit
GCAGGGGATCTCTCTTTACTGCCAGTGATTACAAAGTGGCTTCTGCTGAGTA1980.1113754872677568No Hit
ATCAGAGGGCTGAGAACCTCAGGACTTGAGGAATGATGCTGCGTTGAGTTCC1980.1113754872677568No Hit
AAAACTGGGTGGAGGCTGGAGGTAGCTGATATAAGATTTATGTTTAACAGAA1970.11081298480680854No Hit
CGTACTGGGATATTTTATTATTTGAATCTAAATTAGTAATTTCAAATCTGTC1970.11081298480680854No Hit
TCATATGGGTCAGAAAAAAAAGTTTAAGTCAGAAAGCTTGTCAGCTCATTGG1960.11025048234586025No Hit
TATACAGGGATTCAGCAAATTAACAAAGCAGGGGTCTACTTTCTTCTCCTGT1960.11025048234586025No Hit
CGTTGAGGGTATGATATGTCCATGCATTGAAGTTTATTTATGAGGAGAGTTA1960.11025048234586025No Hit
AAGTACGGGAGAAAATCATCAGACAAATCCAGATTAAGGGATATTTTGCAAA1950.109687979884912No Hit
TCGGTCGGGCAAATAGATAGAGTTCCATTTGGGCGTGAAAATATTTACCATG1950.109687979884912No Hit
AACTCAGGGAACTTTATTAATAATTAATTAAAATGTAAATGTTAATGATAGC1950.109687979884912No Hit
ACTCGGGGCTGCCTGTTGTACCGTGGTGACGTGGTTCCCAAAGATGTCAATG1950.109687979884912No Hit
AAAATTGGGACTTGGAATAATTTGTTTACTCAAATCTGTAAAGTTTTATATA1950.109687979884912No Hit
CATATTGGGAACAGAGAGAGCTTGGAAAAGAGAATTCAGACCTACCTTCAGT1940.10912547742396372No Hit
TCGGTCGGGCCATAAACATAAAAGAAGTTATATGACACATTTTTCTGTAAGC1930.10856297496301547No Hit
AACATGGGGTGAGGGCTGGCTATTGAGAACTGGAAAGAGTAAAATGATAACT1910.10743797004111892No Hit
GCTTGAGGGGTTTCTAATAGAGTTTTGTTTATTTTTGTTTTTACCAGGTGGC1910.10743797004111892No Hit
TGGTCCGGGATGTTTTGGAAATGTACATATTCAAACAGAAGTGCCTCATTTT1900.10687546758017066No Hit
GTACAGGGAGTCTAAGATTAAGAGTACCATTCTGAACGGTGTAGTATTGAAA1900.10687546758017066No Hit
ATGTTCGGGTAATTAAAGCTGCCTTTCCTGACATCAAGCTTCCAGAGCATGA1890.10631296511922239No Hit
GCGCATGGGTGCAGTAGGGGCTGCCATCAAAAGTTTTGAGAATTCTCTAGGT1890.10631296511922239No Hit
TAGGCTGGGGCAGGAAATCAAAGAGAGAGACCCAGAAGTCAGACTCCAAATT1870.10518796019732587No Hit
TTCGCCGGGAGCTAAACAGTACTTGCATACAATGGACACTAAATTTATATTT1870.10518796019732587No Hit
TTGTTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1860.10462545773637759No Hit
CTTGTCGGGGAAACAAGCATAGAAATGGCAGTAGTAAGTTTTTACTTATCAG1860.10462545773637759No Hit
ATGCCTGGGTTACCAAGTTTATATGTGACAGTTGAAAAAGAAATTCCCTTGA1860.10462545773637759No Hit
TATTATGGGGACCTTGTCAGATGATTGCTGACTTTCCATCAAGGATACTTAA1850.10406295527542933No Hit
ATATGTGGGATTTGAGGAAGTGACTTTTTTTGTTTGGGTTAAGTAAAAAGCC1850.10406295527542933No Hit
CTAGGTCCGGGAACATGAAAATTGTTTAGTTTTTCTTGGTTTCATAAACATT1850.10406295527542933No Hit
GGCGACGGGATTGAGAAATTCTCAGTGATTCTGCAATGGATTTTTTTTTAAC1850.10406295527542933No Hit
TCTCTCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1850.10406295527542933No Hit
CGTTCCGGGTGCTGCATATCGAGCTAAAGGAAAGCCTGATGCAGCAAAAAAG1850.10406295527542933No Hit
ACGTTAGGGGGTCACATCTCAGAATTTGATAGGAGATTTTTCTCATTATTGT1840.10350045281448106No Hit
TGTAAAGGGGAGATAGTCTTATAAGAATCACGATTTTCTACACCTGTCATTG1840.10350045281448106No Hit
CTGTATGGGGGCCGCTTGCATGACCCTGACATCGGCAGCGGGAGCGGCGGCC1840.10350045281448106No Hit
GGCTAAGGGGCTTTATCAGGTTCATAAAGAAAATCAGTACTGTAGTTTCTCT1830.1029379503535328No Hit
TCATATGGGTGATGCCCAGCAGGTAAAATGGGCTTAATGCTCTTTTGTTTTT1830.1029379503535328No Hit
TGTGCTGGGGTTCTCTGATAAGGCTCTTGGAATCCCTATTTTTGACAAGCAT1820.10237544789258454No Hit
ATTCTTGGGTACTAACTTACTCTGAACTAGTTTATAGTTACTAACCCCACTG1820.10237544789258454No Hit
TGTTACGGGGCACATGAATTTAATTTTTTGAAAATTTATTTAAGTAGTCTAG1820.10237544789258454No Hit
GCTCTCAGGGGACAGGTCATCCAGTCAGGAAATTAATAAAGAAACATCAGAT1820.10237544789258454No Hit
AGCATAAGGGATGAAATGAAAGTAAAATCTCACAATGGTGAAAGACTTCTGG1810.10181294543163627No Hit
TTAGGCGGGTATATATCGATTAAAAAAGATTTCAAATTCAACATGCTAAATT1810.10181294543163627No Hit
TTTTGTGGGGTACACCTGACCTACAGTGTTCCTATAATGAAAAGTGGTTGCA1810.10181294543163627No Hit
GTGCCGGGGGTGTCCTCATGCCCAAGCCATTCTGCCAGATGCTTTCCATCTA1810.10181294543163627No Hit
TCTGTTGGGTGTATCCTAGCTGACAAATTATTGATTAATAAGAACTTGAATT1800.10125044297068798No Hit
CCGTCAGGGACCAGTAAGCTGGACTGCTGAGAAGAATAAATAACAGTTCCTA1800.10125044297068798No Hit
TGCTGTGGGCAGATGAAGGAATGTTTTATAGTAATTTAATTTGCTCCTGTAG1800.10125044297068798No Hit
TTCGCAGGGATGAGATGAAATGATGAGATGAGATGAAATATGAGATGAAATG1800.10125044297068798No Hit
CCTAAAGGGGAAACATATGACCAACCATTTTGCTAATTGGATGATAATTCAT1790.10068794050973973No Hit
GAATTGGGGGGTCAGTGTTCCTTTCAAACATGTGAGTTCTTTAACAAAAATG1790.10068794050973973No Hit
AGAAACGGGTATCAGATTCTTCTCTTTGAAGAACACGTTGATTAGACGTTCT1790.10068794050973973No Hit
AGGAACGGGAGGAGGGAGAAAACAACCAAATCTCCAAACAGGCCAGTTTTAA1780.10012543804879147No Hit
GAAACGGGGATGCAATGAAAGATGAAATGATGAGATGAAGTGAAATGAAGAA1780.10012543804879147No Hit
GTACGGGATTTCTAGATGTTTGTTTCAAGACCAGCTGTTTCAGATCTATTAA1780.10012543804879147No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)