FastQCFastQC Report
Sat 31 Dec 2022
SRR5846735.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846735.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173801
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGCCGTCT11500.6616762849465768TruSeq Adapter, Index 7 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGC9690.5575341914028112No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTTCTCGGGCCTGAGTTC2920.1680082393081743Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGCATCGGGAGTCTTTGCAAAGAATGAAGAAGTGAATTCGCTGTACATTTGT2640.15189786019643153No Hit
TTAGTTGGGGATGGGGCTGTGGCATCCAAATCCACAAAATGGAAATAACTAT2420.13923970518006223No Hit
AAGCTTGGGGGATATGTGACATAAGTGAGGTGAGTCATGGCTAGCATTCATT2270.13060914494162865No Hit
CTAGATGGGTGTTTCCATTTGAAAGGAACTGTAAGCTTTTATCTTTTAACCA2250.12945840357650418No Hit
AACACAGGGGAACTAATGGTAAGACACCAAACCATATCCAGGAAGCTAAAAG2240.12888303289394193No Hit
TCATATGGGTCAGAAAAAAAAGTTTAAGTCAGAAAGCTTGTCAGCTCATTGG2230.12830766221137968No Hit
GCTCTTGGGGAAATATAACTTTTGTCAAAGAAATACTCTCAGAAGAGAAATG2180.12543080879856847No Hit
ACTTCAGGGCCTCAGTTTATAAAATTCTATAATTCTAAACACAGCAGTAATG2170.12485543811600623No Hit
TTTGCAGGGTGGTATCTCACTGTGGTTTTGATTTACATTTCTCTAATGACCA2140.12312932606831951No Hit
TCGTTAGGGTTAGCAGCCGTGGCAACTGCCAAGGACACATACAACAAAAAAA2100.12082784333807056No Hit
GACTTGGGGTCTGATATCCAGGTGAAAGAACTGGAGAAGCGTGCCTCAGGCC2080.11967710197294608No Hit
CGATGAGGGGGCCAAAAACAAAATTGTAAAGGAAATTTAAATTCTGGAGAAT2070.11910173129038382No Hit
TAAGTTGGGGAATCAACCCATAGAAGTGACAGCGCTGTATTCATTTGAAGGA2030.11680024856013486No Hit
GTTCGGGGAGTCTCTTCAGTAAATGATGTTGGAAAAACTTGGTATCCACATG2010.11564950719501038No Hit
GCAGGGGATCTCTCTTTACTGCCAGTGATTACAAAGTGGCTTCTGCTGAGTA2010.11564950719501038No Hit
CGTTGAGGGTATGATATGTCCATGCATTGAAGTTTATTTATGAGGAGAGTTA2010.11564950719501038No Hit
CGTTCCGGGTGCTGCATATCGAGCTAAAGGAAAGCCTGATGCAGCAAAAAAG1990.11449876582988591No Hit
CATCAAGGGGTGAAAATAGAAGTTTGGCTGCTAAATAAAAGAAAATTATCTC1980.11392339514732366No Hit
GAAATAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1960.1127726537821992No Hit
CTTGTCGGGGAAACAAGCATAGAAATGGCAGTAGTAAGTTTTTACTTATCAG1960.1127726537821992No Hit
CGTACTGGGATATTTTATTATTTGAATCTAAATTAGTAATTTCAAATCTGTC1960.1127726537821992No Hit
AGCATAAGGGATGAAATGAAAGTAAAATCTCACAATGGTGAAAGACTTCTGG1950.11219728309963693No Hit
CCGTCAGGGACCAGTAAGCTGGACTGCTGAGAAGAATAAATAACAGTTCCTA1930.11104654173451245No Hit
CTAGGTCCGGGAACATGAAAATTGTTTAGTTTTTCTTGGTTTCATAAACATT1920.11047117105195021No Hit
GGCTAAGGGGCTTTATCAGGTTCATAAAGAAAATCAGTACTGTAGTTTCTCT1910.10989580036938798No Hit
GGCATGGGGTTCCTTGACATTGTTCCATTAACTTGTGTTCTGAAACTTCTGT1910.10989580036938798No Hit
TGTACTGGGCATGCCATAATCTTGCAGTTTTCTGGGACATCTAGGTACTTTG1910.10989580036938798No Hit
TGCGAAGGGTATTCACCTCTAATACCCTTGATTAGGTTACATTGCCATTAGT1900.10932042968682573No Hit
TGTAAAGGGGAGATAGTCTTATAAGAATCACGATTTTCTACACCTGTCATTG1900.10932042968682573No Hit
TTGTTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1880.10816968832170126No Hit
TCGGTCGGGCCATAAACATAAAAGAAGTTATATGACACATTTTTCTGTAAGC1880.10816968832170126No Hit
ATTAAAGGCGAGTAGATTTCAAAAACAGAGATTTTAAAAATTTGCTTGCTTT1880.10816968832170126No Hit
GTACTAGGGATACTTTAAAACAACACGAACAACTGGAGTTGACTCAAAAAGA1870.10759431763913901No Hit
CATTGTGGGACGCGCAGGAAACATGGCGGCGGCGGGTGTTGTGAGCGGGAAG1870.10759431763913901No Hit
CATATTGGGAACAGAGAGAGCTTGGAAAAGAGAATTCAGACCTACCTTCAGT1870.10759431763913901No Hit
TATACAGGGATTCAGCAAATTAACAAAGCAGGGGTCTACTTTCTTCTCCTGT1870.10759431763913901No Hit
GCAACATGGGTGTTTGAAAAATTTTTAATGCTGACTGAAGCTTGCCATTTGA1870.10759431763913901No Hit
CCCCCTGGGCACAGACACCACTGCCCCATTGAGCACATATCTGATGAAATTA1860.10701894695657678No Hit
GCCTTGGGCGCTTCCCACCTGCCCCGTCCCCCTAGAGACTCAGAGGACCCAC1860.10701894695657678No Hit
CGTTACGGGTCTACATCAGGGATGCACACACACATTCAGTATTTTAAAAGAA1850.10644357627401455No Hit
AGAAACGGGTATCAGATTCTTCTCTTTGAAGAACACGTTGATTAGACGTTCT1850.10644357627401455No Hit
ACATCCGGGATATCCTATGTCTTTTACCTTCCTGCTTTGCTCATTTAACTGC1830.10529283490889005No Hit
ATGTTCGGGTAATTAAAGCTGCCTTTCCTGACATCAAGCTTCCAGAGCATGA1830.10529283490889005No Hit
TCTCTCGGGGTGAGCTGCCACTGGATTTAGAGAAGAGCAAATGTGACTTTTG1830.10529283490889005No Hit
GTAGGCGGGATTATAAAAGGCCATTTCCCAAATCTAGAATTTATTACTAAAA1830.10529283490889005No Hit
GTCTCTGGGTGCTAAATGAGTATTACAACTGGCTAATATCATTTTTTATATA1830.10529283490889005No Hit
AAAGACGGGACGTGCTAAGACCCGAGTATCGTGTTTAATTGCAGTGTAGGCA1820.1047174642263278No Hit
TCCTTAGGGCAATTCAGAAGAATAAAACCCCTTTGCGTGACACGATATGGGG1820.1047174642263278No Hit
TTTAGGCGGGAGTTCAAGCGACCAAAGGGTGGGTGGAAGGTGCTGCAGTATG1820.1047174642263278No Hit
GTCAAAGGGATGAGAAGTATAATATTTATTCTTCTTCCCATTCACTCCCACC1810.10414209354376557No Hit
ACTGTTGGGAGCCTCACTAAGTGATTTCATAATCTTACCCTTGGGAGGTTAA1810.10414209354376557No Hit
GAACAAGGGTAAATAAAGATGGGACCCCGAGAGAAGGGACTAGGACTAAACG1810.10414209354376557No Hit
TGTTACGGGGCACATGAATTTAATTTTTTGAAAATTTATTTAAGTAGTCTAG1810.10414209354376557No Hit
TTCGCAGGGATGAGATGAAATGATGAGATGAGATGAAATATGAGATGAAATG1810.10414209354376557No Hit
GCATCGGGGACTAGCATACGTTTTTCTTAATTGTTATCAGCATTGACCAAGT1810.10414209354376557No Hit
TGTTGCGGGATATAATAAACAGTAAACGTTGAGGTTCATCAATAAAAGCACA1800.10356672286120333No Hit
AAAACTGGGTGGAGGCTGGAGGTAGCTGATATAAGATTTATGTTTAACAGAA1790.10299135217864108No Hit
TGCTGTGGGCAGATGAAGGAATGTTTTATAGTAATTTAATTTGCTCCTGTAG1780.10241598149607885No Hit
TGAACTGGGGGCGAGAGCAGGGTTTGATGAGTGCATTCTTGGGATCACCTGA1780.10241598149607885No Hit
GCTTGAGGGGTTTCTAATAGAGTTTTGTTTATTTTTGTTTTTACCAGGTGGC1780.10241598149607885No Hit
TCGGTCGGGCAAATAGATAGAGTTCCATTTGGGCGTGAAAATATTTACCATG1770.10184061081351661No Hit
TAAACTGGGGATGAAATACAAATTACAATTGTATTAACTTCACCAAACATGC1770.10184061081351661No Hit
TCATATGGGTGATGCCCAGCAGGTAAAATGGGCTTAATGCTCTTTTGTTTTT1770.10184061081351661No Hit
AAGCTTGGGTGACAGAGCGAGACTCCGTCTCAAAAAGAAAAGAAAAGATAAG1770.10184061081351661No Hit
TATTACGGGCACTCTGTCCGTCTTCTGCAGCAGCCAGGCAGAGTGCCGACTC1770.10184061081351661No Hit
GTGTTTGGGAGTGAGACCCTATTTCAAAAAAGAAAAGAAAAGAAATTGAAGA1770.10184061081351661No Hit
TCAATAGGGGAGATAATTTATGCTTATTGGAGTCCTAGTATAGAGGTGGTGC1770.10184061081351661No Hit
TCTGTTGGGTGTATCCTAGCTGACAAATTATTGATTAATAAGAACTTGAATT1760.10126524013095436No Hit
AGTTCAGGGCCAAACATTTAAGGAAAAAAATACCACTCCAATATGACACTTC1760.10126524013095436No Hit
CTGTATGGGGGCCGCTTGCATGACCCTGACATCGGCAGCGGGAGCGGCGGCC1760.10126524013095436No Hit
TGTGCTGGGGTTCTCTGATAAGGCTCTTGGAATCCCTATTTTTGACAAGCAT1750.10068986944839213No Hit
GGTACGGGGATAGCAAGCAGCCTGACAACTATATCTGAAGTTTGGCTTAGGG1750.10068986944839213No Hit
ATATGTGGGATTTGAGGAAGTGACTTTTTTTGTTTGGGTTAAGTAAAAAGCC1740.1001144987658299No Hit
TGCGCGCGGGAGCAGCTAAAAGAACAGTCCAATTTAGGATGAGATTTTTTTG1740.1001144987658299No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)