FastQCFastQC Report
Sat 31 Dec 2022
SRR5846734.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846734.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211878
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGCCGTCT16200.7645909438450429TruSeq Adapter, Index 7 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGC15800.7457121551081283No Hit
CTTCACGGGATCCATGGACAATGTTTAAGAAACAAGTGCTTCATTTCTATTG2670.12601591481890523No Hit
TGCCCTGGACAAAACATATACATCAACTATCAGAAGACTCTGAAAAGTTGGA2590.12224015707152228No Hit
ATCCGGGTATGAGGCATAATTATAACAAGCTCCATCTGCCTACGACAAACAG2340.11044091411095064No Hit
GAAACAGGGAATGTACTTTTACATATTTGTAAAAACAATTTTTGGTAAAATG2310.10902500495568206No Hit
CATGTGGGGAATTTCTCATAACCAGAAAATCTTTATACCTAAGACTAAATCT2270.10713712608199058No Hit
GTCCAGGGTCTACTGTCGGGATGCATTCATATTTCTAATCCGCAGCCAAGAA2240.10572121692672198No Hit
ACGCTAGGGCAGTTCTTTCCTCTTGTCAACTGTTTAATGTCCATCCCCAGAG2180.10288939861618479No Hit
GATAAAGGGTCCGATCTGCGGAGGGGATAAGGTGAAGCCAAGGAAGGGTTTC2170.10241742889776193No Hit
GGTTAGGGGAGGACGCCAAGTGGCGGGTGCGGCTGGCCATCATTGAGTACAT2170.10241742889776193No Hit
ATGTCTGGGAGACGGAGCCACTGTGAAGGCGGCAGGTCTCAGTCCCCTGGTC2170.10241742889776193No Hit
TTTAACGGGGGGAGGCTGCCGGCGTGGACCGCGGGAAGGCGGGGCTGGGGCT2140.10100151974249331No Hit
TTTGTTGGGTGAAAAAATGCTGAACATCACTAATGATCAGGGAAATGCAAAT2140.10100151974249331No Hit
TAATACGGGAGGAGTAGGTTTTCAAATGTCCCTATTCCACTATTCGGAAAGT2140.10100151974249331No Hit
AACGCAAGGGACACATCTGAAAGTCAATCAGTGGACTTGGTCTAGAGTCTGA2120.1000575803056476No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)