FastQCFastQC Report
Sat 31 Dec 2022
SRR5846733.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846733.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213611
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGCCGTCT16130.7551109259354621TruSeq Adapter, Index 7 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGC15870.742939268108852No Hit
CTTCACGGGATCCATGGACAATGTTTAAGAAACAAGTGCTTCATTTCTATTG2800.1310793919788775No Hit
GTCCAGGGTCTACTGTCGGGATGCATTCATATTTCTAATCCGCAGCCAAGAA2490.11656703072407319No Hit
TTTGTTGGGTGAAAAAATGCTGAACATCACTAATGATCAGGGAAATGCAAAT2460.11516260866715666No Hit
CTTTGTGGGGAGCGCGAGCCCCGGAGCCCTTGAGCGCGAGGCGCGGAGCCCC2430.1137581866102401No Hit
ATGCGGGCAGCCACAGCCTGGCTTTGGGTACAGCATGTGAGATGAAGCGCTG2430.1137581866102401No Hit
AGATGGGGGGAAGCTGACGGGCATGTGGTGGAAACAGCTGGTGGCCGGCGCA2400.11235376455332356No Hit
TTTAACGGGGGGAGGCTGCCGGCGTGGACCGCGGGAAGGCGGGGCTGGGGCT2390.11188562386768473No Hit
GCGCTGAGGCAGGAAGATGGCTTGAGCCCAGGAGATCGAGGCTGCAGTGAGC2350.11001306112512932No Hit
TAATACGGGAGGAGTAGGTTTTCAAATGTCCCTATTCCACTATTCGGAAAGT2270.10626793564001853No Hit
ATGTCTGGGAGACGGAGCCACTGTGAAGGCGGCAGGTCTCAGTCCCCTGGTC2240.10486351358310199No Hit
GATAAAGGGTCCGATCTGCGGAGGGGATAAGGTGAAGCCAAGGAAGGGTTTC2230.10439537289746316No Hit
TGCCCTGGACAAAACATATACATCAACTATCAGAAGACTCTGAAAAGTTGGA2220.1039272322118243No Hit
AGCCTGGGGAATTAATCAACTATCGGAGCTAGCAGACCCACTTAAGGATGAA2210.10345909152618546No Hit
ATCCGGGTATGAGGCATAATTATAACAAGCTCCATCTGCCTACGACAAACAG2200.1029909508405466No Hit
GTCAGTGGGAAGATAATAGTTTGCAGAGAAATTCAGTGAAGCAGCTGATAAA2180.1020546694692689No Hit
GGTTAGGGGAGGACGCCAAGTGGCGGGTGCGGCTGGCCATCATTGAGTACAT2180.1020546694692689No Hit
TAGACAGGGCAGACATTCCTCCCCTAAATATTTAATGTACCCTTTTGAAAAA2180.1020546694692689No Hit
TTAAGTGCGGGATGGAAGATGGAATTTACACCTGTCCAGTTTGTATTAAAAA2170.10158652878363005No Hit
AGCGGGGCAGAAGGAGAGAGATGTTATCATGAGGGAATTCCGGTCAGGGTCA2170.10158652878363005No Hit
GCATCTGGGAGGGAGCTACCACCATTGATATTCAGTAAATAGTTAATAAGGA2160.1011183880979912No Hit
TTACGATGGGACACAAAAATAATTTTCCCTTTTCCATTCTCAAAAATGATTT2140.10018210672671352No Hit
TAGCGCGGGGGGAAAGGCACTAAGAAAAGATGAACCTGCAGTCCCTGTTATA2140.10018210672671352No Hit
CCCAAAGGGGAAATAAAAGTTCTTAAGTGGTTTTTCCAGAATGAAGAGCCAT2140.10018210672671352No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)