FastQCFastQC Report
Sat 31 Dec 2022
SRR5846732.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846732.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210777
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGCCGTCT16040.7609938465771882TruSeq Adapter, Index 7 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGC15590.7396442685871798No Hit
GAAACAGGGAATGTACTTTTACATATTTGTAAAAACAATTTTTGGTAAAATG2500.11860876661115777No Hit
GCGCTGAGGCAGGAAGATGGCTTGAGCCCAGGAGATCGAGGCTGCAGTGAGC2270.10769676008293126No Hit
ATCCGGGTATGAGGCATAATTATAACAAGCTCCATCTGCCTACGACAAACAG2240.10627345488359735No Hit
CAACGCTGGGACCAACTATGTGCTGCCTTCAAGAGATACACCTAACACATAA2230.10579901981715273No Hit
CTTCACGGGATCCATGGACAATGTTTAAGAAACAAGTGCTTCATTTCTATTG2220.10532458475070809No Hit
GATAAAGGGTCCGATCTGCGGAGGGGATAAGGTGAAGCCAAGGAAGGGTTTC2200.10437571461781882No Hit
TGGTACGGGTCAGCTTCTTGTGTGGTGTCATTGCCATCGCCCTGCCCATCCA2190.10390127955137421No Hit
TTAAGTGCGGGATGGAAGATGGAATTTACACCTGTCCAGTTTGTATTAAAAA2180.10342684448492957No Hit
TGCCCTGGACAAAACATATACATCAACTATCAGAAGACTCTGAAAAGTTGGA2170.10295240941848494No Hit
GAGCAGGGGGGTATGCAGGGATTAGCATTTTATGTTCTCAAGTATGCTGGTG2170.10295240941848494No Hit
ATGCGGGCAGCCACAGCCTGGCTTTGGGTACAGCATGTGAGATGAAGCGCTG2150.10200353928559569No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)