FastQCFastQC Report
Sat 31 Dec 2022
SRR5846731.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846731.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172492
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGC14730.8539526470792848No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGCCGTCT13580.7872828884817846TruSeq Adapter, Index 7 (95% over 21bp)
TAGCTGGGCAAGCCAAAGGACCATCTCTTCTTTCTTCCACCTCCCTTCACCC2410.13971662453910905No Hit
TTGCGAGGGTGTGATTTCTTTAGCAGAGAAAGTTGGTTTTAAAAATAAATAG2280.13218004313243514No Hit
AGCCTTGGGAGCGAAACAGATAGATAAATAAGAGATACAATCTAGGTAACTT2270.1316003061011525No Hit
GAACATGGGATAGCATCTTTGGTAGATGGAGAGAGATCTTTTATGACAAAGA2240.1298610950073047No Hit
GTCGCGGGGTAACAGTAATGGGGTTTGAGAATGTTATTACTTTTTGATTATT2230.12928135797602208No Hit
CAAGCCGGGGCGGCCGGATGGGGAAGGCGGCGGCGGCGGTGGCCTTTGGGGC2180.12638267281960902No Hit
TATGCTGGGGTAAGGGGCCAATTCTAAGTATTCTTCACGGCAGCCAGAAGTT2120.12290425063191336No Hit
CGTCGCGGGAGTAAAAAGGGAATAAAACTAGATGAGTGGTTTTCAAACTTTA2110.12232451360063075No Hit
CATAATGGGGAATCATGAAAGCACAAGCGTACCAAACGGGCAAGGACATCTC2110.12232451360063075No Hit
TTGACTGGGGCGCATGATGAAACTTCGGCTCACTCCTTGGCGCCTGCCTGAT2100.12174477656934815No Hit
GACGAAGGGTAAGTGGTAAATGAAAGTGGACTTTGATTATTTCTACTATAAA2100.12174477656934815No Hit
GCTAAAGGGCTCTTCCGATCTCTTGATGGGTATTCCTAATATGCCCACTTGG2090.12116503953806554No Hit
GTGAAGGGCGAACCTTAAATTTGGATTGAAATCAGGTGCTGTCAGAGTGTTG2070.1200055654755003No Hit
CTTTGCGGGCAAATATAACATCTTAGTCAAAGCCTTGGTAATATAACCAATG2070.1200055654755003No Hit
AGGCAGGGGATTAAAGTCATATTGGGAAAGATGGCAAAGCTTTTGAGAATAT2070.1200055654755003No Hit
TTGTCTGGGACATGATGCTTTGAAGACATATGCATCATTGGCTACACTTCCA2060.1194258284442177No Hit
GAACCAGGGAACTTTGGTCAGGGATTTATTTCCTTTGGAATCTTTGGATTAG2000.11594740625652203No Hit
GTTTCGGGGCTCTAATGGAATGGAATAGAATAGAATGGACTCAAATGAAATG1970.1142081951626742No Hit
ATTTGCGGGACAAGAGTTTATGAATTATACATATAAAATGGCTTGCCTTAGG1960.11362845813139161No Hit
CAGCATGGGAGAGGGTGGCAGAAGTGCTCAATGACCCTGAGAACATGGAGAA1940.1124689840688264No Hit
TACATTGGGATTCAGTGAAGATGCCCTTATTTAGATATGTTGATTTCCTTAT1940.1124689840688264No Hit
TTCTAGGGGAAATATGTACCTTCATTCAGTTCATTTTTGTAAATTGTAACAG1940.1124689840688264No Hit
GCTCCCGGGAAGAGACCTGATGCGGGACCACAAGGCCTGTCTGCCTGATGCC1940.1124689840688264No Hit
CACAATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1920.11130951000626116No Hit
GCATCCGGGGGATGAAAAGGCAAGCTACAGACAGAAAGTATTTGTAAACCAG1920.11130951000626116No Hit
TAACTGGGGTTTGGGACACCTAGGTGGGAGCATCGCTTGAAGCCAGGAGTTC1920.11130951000626116No Hit
AATAGAGGGATTTCTCAGCCTCATGTTCTATTTTAGCTCACACACGACCCAT1920.11130951000626116No Hit
TTGCGTGGGTGTTGGTGTATATCATTATAGAAATCATTTTCTAAAGGAGTGA1910.11072977297497855No Hit
GAACCATGGGCTCATTAGGGGCCTTGAAGTTGATAGTATGCTGGTATTCTAC1910.11072977297497855No Hit
AATGTGGGGATATGATTTTTAAATAAATTGTACAGTAATTTTTCTGTTGAAA1910.11072977297497855No Hit
AGAGGCGGGAAGTGATCCACATGCCTTGGTTGACCAAATTGCTGGGATTACA1890.10957029891241334No Hit
GCACTTGGGCATCTGTGGTGGCTCTCTGCAAGTTTTAAAACTGCCTCTGCTG1870.1084108248498481No Hit
CGAGCAGGGTAAACAAATGTAATTACTTTATTTATTAACTTTTATTTTCAGT1870.1084108248498481No Hit
GCAAAGGCTGACATTTATTTGTTAGAAAATTACACACATTTAAAAATTCAAA1870.1084108248498481No Hit
AACGGAGGGGATAAGCCCAATCTTGTTCAGCACGTTCTTCTAAATGCCTGAC1860.10783108781856549No Hit
TGGCATAGGGAACTGAAAGTTTGTACCCTTTCACCAACTTCTTCCCATTTTC1850.10725135078728289No Hit
AACATAGGGATAGAAAAGGAAGCTCCAAAAGAGATAAACATAGATTTTCAAA1850.10725135078728289No Hit
CTATGCGGGCTGATACATCAGAGGTGAATACCAGCACCTATTAGGTTTCATT1840.10667161375600027No Hit
CATCAGGGGTGGTCAGGCGGCGGCCGTGACAGAGAACGGTGCTGGGGAGAAG1830.10609187672471768No Hit
GCTAGGGGATGCTGCATTGCTTTGATTCATGTTTAAAGACCTAAATTTCTAT1830.10609187672471768No Hit
GTTTGCGGGAAGTTTCAGGCGCACAAACCCGCAAAGAGTAAGACGGCAGCGG1820.10551213969343506No Hit
GTGTGAGGGTAAATACATACTGTTCTAATTTTGTATTTTTTAATTTTTGAAT1820.10551213969343506No Hit
AATGCCGGGACTACAGAATTATTTTTATACACTAGGTAACATGCTAGTCTTA1820.10551213969343506No Hit
CAAATGGGGATATAAAAGATGGTAAGAACTGATTTCCAGAAGGACTTCAAAG1810.10493240266215245No Hit
CTAATAGGGATCTCTCTGCGGGTGGTGCAGCCCTGCGGGGCCTGTGCGAAGA1800.10435266563086983No Hit
CTTCGCGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1800.10435266563086983No Hit
TTCGATGGGGAGCCTTGTTTTCTGCCTTTGTATTTCAGAGGAAAGAGATAAT1800.10435266563086983No Hit
AGATACGGGATGACCCCAATATGAGATTTTTTTTCTGTGACTCTTCCATGTG1800.10435266563086983No Hit
TTTCATGGGTATTAGTCTGTTTTCACACTGCTCATAAAGACAAACCCCTTCT1790.10377292859958723No Hit
TTCAAGGGGATAAACTTTCTACTTAAAAATTTTACTTTTACCCAATAAACTT1790.10377292859958723No Hit
TCCACTGCACCCGACCGATACTCACATTTTTAAACTTTTTTTTTAATTTTAT1790.10377292859958723No Hit
TCGGAGGGCAGACAGTGAAGAAGAAATTAGAGAAGCATTCCGTGTGTTTGAT1780.10319319156830462No Hit
TAGTTTGGGTAAAACTTCCTAGCATGAAGAGTTTCTACCAAGATCTCCACAA1780.10319319156830462No Hit
AGTATAGGGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCGCGAGGCGGAGC1770.102613454537022No Hit
GTTTCAGGGACTCAATTTTTAATAGTTTTTTTAATGTGTTGGCTGACAGTCT1770.102613454537022No Hit
GTTAAAGGGGGAAAACTAAGCATTGGCCTCATGTTCAGTCTTCAGGATATCA1760.1020337175057394No Hit
AATAATAGGATGAATGAGAAATTTACTCTGAGTTTCCTGGCATCTAAAATAG1760.1020337175057394No Hit
TGGCGTGGGATATGTTTATGTATATAAAATTTTACTCTTTTTTTCCAGCTTC1760.1020337175057394No Hit
TTTCGTGGGATTACAGGAGGGACAGTTAAGACACATAAAAACTGGGGAACCA1750.10145398047445679No Hit
TTCTGCGGGAACCACAGGTCTACAAGAGCGTTTTTAGTAAAGTGCCTGTGTT1750.10145398047445679No Hit
TATTAAGGGATCCCAGAATACCACTACTATGTCAATTTAATCTAATTTAATC1750.10145398047445679No Hit
ATTCATGGGACACGGAGAATCGACAGGCTGTAATTTAGCTAAGCTGCCTGCA1740.10087424344317418No Hit
CTTAATGGGGCTCTCGAGTCACTCCGGCGCAGTGTTGGGACTGTCTGGGTAT1740.10087424344317418No Hit
GAGCTGGGTACTGACATCAGTTTGGGTGAGAGAGGGAGTCACTTTACTGAAA1740.10087424344317418No Hit
GTTTCCGGGTACCATTACAAGACACATTGTATACCGAGAACCAAAAAGAAAC1730.10029450641189155No Hit
GTTATGGGCCACCACAAAGCCTGGAATTTGCCATGTTAGCATTTTCAATTCC1730.10029450641189155No Hit
TACGCGTGGGATGCACATTCATCCTCACATGTGGCACGTAAGGTCTCATTTG1730.10029450641189155No Hit
ACAGCTGGGCTGTTGTGTGAGTTTAGTATATTTAATTTACTTTTTGTTACTC1730.10029450641189155No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)