FastQCFastQC Report
Sat 31 Dec 2022
SRR5846729.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846729.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169903
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGCCGTCT13580.7992795889419257TruSeq Adapter, Index 7 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACATCATCTCGTATGC13220.7780910284103283No Hit
TATGCTGGGGTAAGGGGCCAATTCTAAGTATTCTTCACGGCAGCCAGAAGTT2390.14066849908477191No Hit
TTCAAGGGGATAAACTTTCTACTTAAAAATTTTACTTTTACCCAATAAACTT2240.1318399321966063No Hit
GTCGCGGGGTAACAGTAATGGGGTTTGAGAATGTTATTACTTTTTGATTATT2240.1318399321966063No Hit
AGGCAGGGGATTAAAGTCATATTGGGAAAGATGGCAAAGCTTTTGAGAATAT2170.12771993431546236No Hit
CAAGCCGGGGCGGCCGGATGGGGAAGGCGGCGGCGGCGGTGGCCTTTGGGGC2150.12654279206370694No Hit
GTTTGCGGGAAGTTTCAGGCGCACAAACCCGCAAAGAGTAAGACGGCAGCGG2120.12477707868607382No Hit
AATAGAGGGATTTCTCAGCCTCATGTTCTATTTTAGCTCACACACGACCCAT2060.12124565193080757No Hit
TTCTGCGGGAACCACAGGTCTACAAGAGCGTTTTTAGTAAAGTGCCTGTGTT2050.12065708080492989No Hit
GAACCAGGGAACTTTGGTCAGGGATTTATTTCCTTTGGAATCTTTGGATTAG2050.12065708080492989No Hit
GCTAAAGGGCTCTTCCGATCTCTTGATGGGTATTCCTAATATGCCCACTTGG2050.12065708080492989No Hit
ATTTGCGGGACAAGAGTTTATGAATTATACATATAAAATGGCTTGCCTTAGG2020.11889136742729675No Hit
GAACCATGGGCTCATTAGGGGCCTTGAAGTTGATAGTATGCTGGTATTCTAC2020.11889136742729675No Hit
TACGCGTGGGATGCACATTCATCCTCACATGTGGCACGTAAGGTCTCATTTG1990.11712565404966363No Hit
TAACTGGGGTTTGGGACACCTAGGTGGGAGCATCGCTTGAAGCCAGGAGTTC1970.11594851179790823No Hit
GACGAAGGGTAAGTGGTAAATGAAAGTGGACTTTGATTATTTCTACTATAAA1960.11535994067203052No Hit
TAGCTGGGCAAGCCAAAGGACCATCTCTTCTTTCTTCCACCTCCCTTCACCC1960.11535994067203052No Hit
CTCGCTGAGGGTCTCATGAAGACTGACCAACTTCCCTTTGAAAAAGCCTATG1950.1147713695461528No Hit
ACTAAGGGGCATCGTGGGCATAAAATGTTCACCTTTTTTCCAGATGAGTACA1910.11241708504264197No Hit
GTGAAGGGCGAACCTTAAATTTGGATTGAAATCAGGTGCTGTCAGAGTGTTG1910.11241708504264197No Hit
GTTTCGGGGCTCTAATGGAATGGAATAGAATAGAATGGACTCAAATGAAATG1910.11241708504264197No Hit
GCATCCGGGGGATGAAAAGGCAAGCTACAGACAGAAAGTATTTGTAAACCAG1910.11241708504264197No Hit
GTCAGGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1910.11241708504264197No Hit
AGCCTTGGGAGCGAAACAGATAGATAAATAAGAGATACAATCTAGGTAACTT1900.11182851391676428No Hit
TTCGATGGGGAGCCTTGTTTTCTGCCTTTGTATTTCAGAGGAAAGAGATAAT1890.11123994279088657No Hit
CGTCGCGGGAGTAAAAAGGGAATAAAACTAGATGAGTGGTTTTCAAACTTTA1880.11065137166500887No Hit
GTGGCGGGCTTCCCTATAACTAGATAGCTGCTTGTATTACTGGGACTTTTTG1880.11065137166500887No Hit
ATTATGGGGAGCCCGGTAATCGCATAAAACTTAAAACTTTACAGTCAGAGGT1880.11065137166500887No Hit
TTGACTGGGGCGCATGATGAAACTTCGGCTCACTCCTTGGCGCCTGCCTGAT1870.11006280053913114No Hit
GTTTCAGGGACTCAATTTTTAATAGTTTTTTTAATGTGTTGGCTGACAGTCT1860.10947422941325344No Hit
CTCACTGGGAGGAGTCTGTTCACATAGAGACACCTGTAAGTATTTATTACAA1850.10888565828737573No Hit
GCAAAGGCTGACATTTATTTGTTAGAAAATTACACACATTTAAAAATTCAAA1850.10888565828737573No Hit
TCTTATTCGGGGAACATCAATTCAGTCTCTGCTGGATGAACCGAATCCTAAC1840.10829708716149802No Hit
CAGCCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1840.10829708716149802No Hit
TGTGACGGGGGGGAGCTCTTTGAAGACATTGTGGCGAGAGAGTACTACAGCG1820.10711994490974262No Hit
TTGCCAGGGATGGGAATTCAAGATGAGATTTGGGAACAGACACAACCAAACC1820.10711994490974262No Hit
GGCAGAGGAGTGTTGTTTTGACAGATAAACATGGTAAGCTTGGTTGTTTTCT1820.10711994490974262No Hit
CTATGCGGGCTGATACATCAGAGGTGAATACCAGCACCTATTAGGTTTCATT1810.10653137378386492No Hit
CTTGCTGGGATCACTTGTCAACATTAATCTGTTGATTGAAATAGTAAAAATG1810.10653137378386492No Hit
TACATTGGGATTCAGTGAAGATGCCCTTATTTAGATATGTTGATTTCCTTAT1810.10653137378386492No Hit
TTGCGAGGGTGTGATTTCTTTAGCAGAGAAAGTTGGTTTTAAAAATAAATAG1800.10594280265798721No Hit
GAAACCGGGTGACCGCGATGATGTGGCTTTGAAGAACTTTGCCAAATACTTT1800.10594280265798721No Hit
CGCTATGGGACATCACGAGAAACTTTTGCAAGTGACACGAATGAGGCCTTGG1790.10535423153210949No Hit
TCTGGTGGGGAAAAAGAAAAGTAAAAAAACTTCCCAACTCACTTTGTGTTAT1790.10535423153210949No Hit
GCTCCCGGGAAGAGACCTGATGCGGGACCACAAGGCCTGTCTGCCTGATGCC1790.10535423153210949No Hit
ATTCATGGGACACGGAGAATCGACAGGCTGTAATTTAGCTAAGCTGCCTGCA1770.10417708928035407No Hit
AAAGTAGGGGACGTGAGGGCGCGGGTTAGTGTCAAAATAGGAAGAGGAGGCG1770.10417708928035407No Hit
TCGGAGGGCAGACAGTGAAGAAGAAATTAGAGAAGCATTCCGTGTGTTTGAT1760.1035885181544764No Hit
ATTCGAGGGTGAAATAAATATCCATGAGTTTGTAGGGTTAAAATTAATAAAT1760.1035885181544764No Hit
ACAACTGGGGGCTGAGAACGCGGGTCCACGCGTGTGATCGTCCGTGCGTCTA1750.10299994702859867No Hit
CTTCGCGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1750.10299994702859867No Hit
ATTGCAGGGGCGGCGGCTGGAGAGGTGGTCGGAGAAGTAGGAACCTCCTGCC1750.10299994702859867No Hit
TCCACTGCACCCGACCGATACTCACATTTTTAAACTTTTTTTTTAATTTTAT1740.10241137590272097No Hit
AATGCCGGGACTACAGAATTATTTTTATACACTAGGTAACATGCTAGTCTTA1740.10241137590272097No Hit
GTTAAAGGGGGAAAACTAAGCATTGGCCTCATGTTCAGTCTTCAGGATATCA1730.10182280477684326No Hit
ATTGTCGGGATCCGCGCGATGGGCTCTGAGGCGGCGCAGCTGCTGGAGGCTG1730.10182280477684326No Hit
GTTTTGGGCACTTGAAGCCATTCTTACTAAATTATTAGTTTTGTAATTCGGT1730.10182280477684326No Hit
GCTAGGGGATGCTGCATTGCTTTGATTCATGTTTAAAGACCTAAATTTCTAT1730.10182280477684326No Hit
CGAGCAGGGTAAACAAATGTAATTACTTTATTTATTAACTTTTATTTTCAGT1720.10123423365096555No Hit
GAACAAGGGGTTAGAGCTGCTGTAACAACATATCCCAGACCTGGGGACTTCA1710.10064566252508785No Hit
CCAAAAGGGAGAAGAGATCCGAGAAGATAGGAATCCAAGAGATGGACATGAT1710.10064566252508785No Hit
AAACTAGGGGGGAATAGCATTGAATCTATAAATTACTTTAGGCAGTATGGCC1710.10064566252508785No Hit
TCTCCTGGGGACCTTAACTGGAGTTCATCAAGTTCCCACTGAGAATGTGCAG1710.10064566252508785No Hit
CAATGAGGGCTCAGTTTGTGTACAGGAGTGTGCTTTGTGCAACCTCCTGAGA1710.10064566252508785No Hit
AACGGAGGGGATAAGCCCAATCTTGTTCAGCACGTTCTTCTAAATGCCTGAC1700.10005709139921012No Hit
GTTGCTGGGGAACACCATAGTGGTTAAATCCTCTCATTTGATCAGTCTGTAA1700.10005709139921012No Hit
CTGCGTTGGGGCTTCCGATCTGCAAATGGGGCACTTTTAAAAAAATAATTGG1700.10005709139921012No Hit
GCTGCGGGCAGCTTCTGTCATTCATCCATCTCCATGCAATTGCTGCTGGTAT1700.10005709139921012No Hit
AGCAAGGGGGAGATTTCTTGCATAAATTTCTTGAAGTGTATCAGAATATCAG1700.10005709139921012No Hit
TGTAATGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGT1700.10005709139921012No Hit
AAGCGAGGGGTTCCCCATCTGGTACTAATGACCTACTAGCCCTGGTTATTTT1700.10005709139921012No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)