FastQCFastQC Report
Sat 31 Dec 2022
SRR5846701.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846701.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115771
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGCTAAATATCTCGTATGC8430.7281616294236035No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCTAAATATCTCGTATGCCGTCT7640.6599234696081057No Hit
ATAGGCGGTGCTTCTATTCAGCCTGAAGAAGTTATGAAAGATGGATCTTCAT1830.15807067400298866No Hit
TAAAAAGGGAGGTGCTTCCCCTGAGGTGCAGGGGAAGACCAAAGTTTGCAAC1800.1554793514783495No Hit
TACTATGGGAGGTTGGCAAATAGCACTGTTTTCTTAGCTGCAAGAATTCATT1770.15288802895371034No Hit
GAGGCGGGATTTCTTTTTTAGTTCAAAGGTGAAATTTTGACCTTTGTATTAA1770.15288802895371034No Hit
GGTTAAGGGATGTGGGAATGTAAAATGGTGTAGCCGCATTGGAAACAGTTTC1730.14943293225419146No Hit
CCGAGTGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAAC1680.14511406137979285No Hit
CTTCTTGGGGCAAAAACCAACTCCATTTTGCCAGGATTTTGTTGTGCTGAGA1640.14165896468027397No Hit
GCTTACGGGAGTAGAAGAAATAGCAACTGCTTTGATATGGAGATTTACCCCT1610.13906764215563483No Hit
AATGCTGGGATCCCCTTGCCGATGCCCACCTCACCAAGAAGCTACTGGACCT1610.13906764215563483No Hit
TGCGTTGGGATTCTAATTATTTTAACAAGTATCCCTTAGTTTTCATCTTTAA1600.13820386798075512No Hit
GTGACTGGGAACGTTTCGAACGTTTTGCATCCAGCTGTGGTTAACACGGGAT1600.13820386798075512No Hit
GATGCTGGGATGGGGGTCCTTGGCAATATATAAAATTTATAGTAAGAGTTTC1600.13820386798075512No Hit
TAAAGCGGGTGAGAAGGCAGAAATATGCATGTTAAACAATTCCCCAACAAAA1600.13820386798075512No Hit
GGCCTCACTCCGTAGGTCTTATGTTGAAGTCTTGTTTTACTTGTATGCACTT1580.13647631963099568No Hit
GTTTTAGGGATTTAGATTAAATTTTAAATGCAAGAAACACTTTCGAAATTAC1570.13561254545611595No Hit
AATACTGGGAGGAAGGAATATGTGGAGAGGGCCATTGAATATCGACTTCATG1560.13474877128123625No Hit
TCGCCGGGGCATCACTATCGGAGAAAAACTTCATGTGCACAAAAGCACCAGT1560.13474877128123625No Hit
ATGCCAGGGCCACTCACACTTTCTCCGTATCAGCAGTAAAGGGCTGTTTCAG1540.1330212229314768No Hit
AGACCAGGGAATACTTCCGAATTAAATTATTTAATATTTGACTGATTTCAAT1540.1330212229314768No Hit
TTAGAGGGCACAGTAGAACGTGAAATTAAGTAGAGTCCCCTTTTAAACAAAT1540.1330212229314768No Hit
CAAGCGGGCACATTGGCCTAAAACCATCAGGCAATTTAAATATGAGATCCCT1540.1330212229314768No Hit
GATGGGATGGCCGCCGTAGTCCAGCTCGGGCTGGTCGCCGATGCAGACGAAG1530.13215744875659707No Hit
TCTGATGGGGGGTGCAGGGGAACCCCAGTCCCCGGACGAATTGCTCCCGAAA1530.13215744875659707No Hit
AAACTGGGGGAATGTGGGAAAGCCTTTAGTGATTTCTATTACTTTCGAAATC1520.13129367458171737No Hit
ATCCGAGGGGTGTTACCAGTGGATTTGAGGAATTAAAATGTCACAGTTTCAT1510.13042990040683763No Hit
TTGTTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1490.1287023520570782No Hit
GTTGTTGGGGAATGGATAAAGAAATTGTGGTGTCTCTATTCACAATGGAGTA1480.12783857788219846No Hit
TACGCAGGGAGTTGAGAAAGAAGAGAAGAAGAAAATCAACCAGCAGGAAAGT1480.12783857788219846No Hit
ACCTGAGGGATATTAAAAATTTAGTGACTATGGTAAGCTTTTGTTCATTTGT1470.12697480370731876No Hit
ACTCCTGGGATTCAGTAAAGTTTCAGGATGCAAAATTAATGTACAGAAGTCA1470.12697480370731876No Hit
GGGCAGGGGCGCACCAACCTAACTGTCTTAAAGTTTAAATACATGATACTTG1470.12697480370731876No Hit
GAGCTGGGAGAAGGCTTTTCTGTAATCCAGGTGAGAGGCGACAGTGGCGATG1470.12697480370731876No Hit
GCTCACGGGAGGATGATGTAATTAAAAAATGGTCAGTGTGCAAAGAAAAGAA1470.12697480370731876No Hit
TTTATAGGGGAACATTAGTATGTTGGTGTAGGTTTCTCTTGGTTCCCAAATC1460.12611102953243902No Hit
TCGTAAGGGATTAAAAAACTCTTCTTACATATAAAAAGCATATATTAATGTT1460.12611102953243902No Hit
ACATCAGGGGTGGAAAAGCATTCCATAATTTCTCTTCTTTGCAAATACATGA1450.12524725535755932No Hit
TAACAGGGAACATAATATGTTGTCTTTTATATTTTTATTTAAATCTGGATTA1440.1243834811826796No Hit
TGGATTGGGACTCAATTCTACCATAATTATGTATATGAGAAAAATATTTTTA1440.1243834811826796No Hit
TGCAAAGGGTATGTAAAAATGAAGGTGGCACCGTGGTGAGACCTAATGAGAA1440.1243834811826796No Hit
CTTTAAGGGCAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAAC1430.12351970700779989No Hit
ACAAAAGGGAGTGCCGAGGGCTTTTGAGTGAAATGCCAGTCATGAAGGTGCT1410.12179215865804044No Hit
GACCAGGGGCTGACTGAATTTTAAGGCATTTGGGTAGCACATATGACAACTG1410.12179215865804044No Hit
GTTCACGGGAAGGAATATTGGCACTCATTTTAGGTAACAATATTTTGTTACC1410.12179215865804044No Hit
TATAACGGGCAATGAATAATTTCCAAATAATTTCATTAGATAATTGAGGCTT1410.12179215865804044No Hit
GCGTAAGGGCAGCCCAGCCAACATGGCGAAACCATGTCTCTACTAAAAATAC1410.12179215865804044No Hit
TTATGGGCATAGAGAGAATATGGCATTTGAAAGAAATCGTTTTCCTTGGATC1400.12092838448316072No Hit
TGTAGGGTCTACGTGCGGGTGACAAATGTTCTTCCAAAGAACATTGAATACA1400.12092838448316072No Hit
ATATCCGGGCTTGACGTGGGGGGTTAATTCATGATGTTTGCCCTTTTAATAA1400.12092838448316072No Hit
TCTCCGGGGGGACAAGAGACTAAGTTCTAGTCAAAGGAACACACACGGAAGT1400.12092838448316072No Hit
TTTGTAGGGGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT1390.120064610308281No Hit
CAACGCGGGACCTTTATCTTGGTCTTTGTTGATTTGGGTTTTGTTGGGGTTT1390.120064610308281No Hit
AGACTTGGGATAAAACCAAATGTGTTTGAATCTTATAATTCTTCCAAGGCCT1390.120064610308281No Hit
TTTGCAGGGAGACTAAGGTGTTAAATGACTTGTTCACAATCTTTCCACTACC1380.1192008361334013No Hit
TGTTTAGGGGATCTAATGCGGGGAAAGTACCTGTATCAAAGGAATTTTAACA1380.1192008361334013No Hit
CGTATAGGGCTGAGATCTGGAGTCACGTATGTGTGTTAGAGTTCCCTCTTCA1380.1192008361334013No Hit
TGGCGGGTGTGGTAGACTGATTTGTACTCCTTTGGGTATATAACCAGTAATG1380.1192008361334013No Hit
TTTGGCGGGGTGGTTCCTGTCTTTAGTTCTGTTTATGTGATGAGTCACATTT1370.11833706195852156No Hit
TTACTGGGGCGTTTTACAGAAGTAGAAAGAGTGGTTTGGAGAGGTTAAGTAA1360.11747328778364186No Hit
TTGCATGGGATGGACATAATAGTTCACTTACAAATGTGATTTGATGTTAACA1360.11747328778364186No Hit
TCTGATGGGGTAACAATTTCTTGCTTATACTAACTGATGAGTGTCTCACATT1360.11747328778364186No Hit
CGAACAGGGTGCTCCAGTGCTGATGGAGGCCAAAGACAACCCCAGGGTTTTC1360.11747328778364186No Hit
GACTAAGGGCAAAACTTGTATGATACAGCCATAGGATTTCTCAGAAGGGAAT1360.11747328778364186No Hit
AGATGTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1350.11660951360876212No Hit
AGCACTGGGATTCTTTTAAAATTTCCTCTGATACTATAAGCTTTTTGCATTT1350.11660951360876212No Hit
GATATAGGGGGTCCATCTGCCCTCCATAGAAAAATGTCTCTGGCTCATAAAA1350.11660951360876212No Hit
TACAACGGGATACTGGGCTAACAATGAGATAAAATAAAAATACCTTTGAATA1340.11574573943388242No Hit
ACGTCAGGGCTTCACCTACCCCAGTGAATCATCTTTATATTTTACTTGGGAA1340.11574573943388242No Hit
TGATTGGGGAAGCAACAGGCTCACTGGTCACGGATTTGTGTCTGTGACTTTT1340.11574573943388242No Hit
CGACTAGGGAGAGCATTTGTATCTACAATACATTTTAAAGTCAGAGTTCGTG1330.11488196525900268No Hit
TGAACAGGGATACTAAAGTGAGATGCATTTTTTCTCATTTTCAGCAAGACTC1330.11488196525900268No Hit
GCTCATGGGCAGGAAATCAAATCAGTCTTCCGGTCTGCAGCACGGAAAAGCA1320.11401819108412296No Hit
AGTTCAGGGAATGGAAGATGTGCTTGGTGAATATTATGCATGTTCTTGAAGA1320.11401819108412296No Hit
GTATACGGGATATTAAAAATTGGAGAATAAGTGTGCATCCATTGATATTTGG1320.11401819108412296No Hit
CAATGCGGGATAGAATACATTTACAGGAATGTTTATTGCAACATTGTTTAAA1320.11401819108412296No Hit
GTTTGTAATTATTTTCCAACAATAAATAAATTGAATTTATTTCATTAGATAT1320.11401819108412296No Hit
CTATGGGGGGAAATATTAGCAAATTGAATCAAAAAATGTGTTAAAAGAATTT1320.11401819108412296No Hit
ACAAGGGATCTGAAAGCACAAGGTGATACTCATTTTTATGGTCTTCCCATTT1310.11315441690924324No Hit
GTTTATGGGGTGCTTCGAGAGTGTATACATGTCCCATTTCAGATTTATATAT1310.11315441690924324No Hit
GCTTTAGGGATGGTTGAACACTGGTAACTAAAACTATGGAAAATGAAACTGT1300.11229064273436352No Hit
GAGCAAGGGGATCTTACAGCCTACAGAATGCTCTCTGGCTCCCGGCTTTGCC1300.11229064273436352No Hit
AAACAGGGGTATGGCTGGGCATCTTAAAAAGAAATTTTCTTTGGCTAGCCTC1290.11142686855948382No Hit
GCTAATGGGGGAAATGAAAATGTATTAATGTAGTGTTAGTAGAGGTTTTGAC1290.11142686855948382No Hit
AACCGGGGATTGCACGTGAAATGGGATGGTACGTTTTATGATAGTGGATCAG1290.11142686855948382No Hit
TAGTGTGGGCTACTTTAAAAGTTTTAATGGATTGCACCTCTGTAAACTATTC1280.11056309438460409No Hit
GAACAAGGGATTCATCACTAGTTCATTTTTCCCAACTCTCTATCAGATTTGG1280.11056309438460409No Hit
TAACAAGGGAGAAATATTACACTCTACAAAATTGTTTTATCAAGGTCCAAAA1280.11056309438460409No Hit
TATGCCGGGGGGACTGAAAATAAAAGGTTTTTAGTAGCTTAATTAGAAACTA1280.11056309438460409No Hit
CAAAAAGGGAGAGAGCATCTGCAACAAAAAGTCATTTTTCTGGAGTGGAAAA1280.11056309438460409No Hit
AAACAGGGGCAAACACTGAAAAGAGAACAATGATCTGATTTGCCAACGATTC1270.10969932020972438No Hit
AGGACTGGGACTTTGTAACCAGGGAGGTGATGCAGCGTGACATAGCAGCCGG1270.10969932020972438No Hit
GTGTTAGGGAAGAAGCTGGGATTGCAGGCACCAACCACCATGCCTGGCTATT1270.10969932020972438No Hit
ACTGAGGGAAATTCAACATCTTATATAGGTGCAGTTCCTGATGCTCCAAAAC1270.10969932020972438No Hit
ATGCAGGGAACAAATTATTAAAGCAGAAACTCTATCACTTCTTGGAACTAAA1260.10883554603484465No Hit
TAAAACGGGAGTTGATTTTAAGGCAAGAAATACAACTAGAGATAAAGTGCTC1260.10883554603484465No Hit
TAGAGCGGGCTTGTTAGCTTTGCTTTTCTCCCCATATCCCAAGGCGAAGCGC1260.10883554603484465No Hit
GGTGCTGGGGGCAGTAGCAGGACCGCTGCTGTGGAGCTGGTCGCAGGCGGTG1250.10797177185996494No Hit
TATAAAGGGATGTACAAGACTTATATCTACATTTTTTGATCACCAGTTGTAC1250.10797177185996494No Hit
TGATAAGGGTATCTATATGTAAGTTACTAACATTATTATTTTGTCAGATGGA1250.10797177185996494No Hit
CATCAGGGGAGTGATGGTAAACTTTGTGTTGCTCTTTATGAAATGATCCATA1250.10797177185996494No Hit
TTGCTCGGGGCGATCTCAATGCGGGCTACTGAAAAATTTACATTTTGAGTCA1240.10710799768508521No Hit
CTACAAGGGCAAAGCATTTGAGTGAAATTAGTCCACAAGAGATAGTAATAAA1240.10710799768508521No Hit
TTGCAGGGGGAGGTGGAATTGAAGACAGGAGGAAATAATTCAAATCAGGTTT1230.10624422351020549No Hit
AATCATGGGCCTACAGCTGGTTTTACACACACACGTTAAGAGTGATATAAAT1220.10538044933532578No Hit
AAACCAGGGACTTCGAGACTATGTGGATCATTCTGAGACTCTGCAGAAGTTG1220.10538044933532578No Hit
GTCCAAGGGAAGTTGAAAGTTCTACAAATCTCTAGTGCAGGGGCCAAATGCT1220.10538044933532578No Hit
CCAACAGGGTATGCTTTGCAGAAAAGATTCTTTGTATTGGAAATTAATAAGA1220.10538044933532578No Hit
CAACCTGGGGAAGAGTATGTACCAGTGTTTTGGTTACATAGTGGGAAAAATA1220.10538044933532578No Hit
ATAGCCGGGACTTTTCTTTGTGAAAATAAAAATGAAAAGATTTAAAAAGAAG1220.10538044933532578No Hit
AAAAGACAAGAATCAGAGCCCTGTCTTCATTATCTATATCCCCAGAGCCTGG1210.10451667516044605No Hit
GCACTTGGGGTCCTTTCTATAAGCTTGTCACTGCAAAAAGTTGCCTTTTGCT1210.10451667516044605No Hit
GGCATAGGGATGTGCTTACATGTTTAATATCTGCCTCACTTTTGAATGAAAG1210.10451667516044605No Hit
AGTCCAGGGTTAATACTTCAGAGCTAATTCAAGAAACCTGTGTGCATTAACG1210.10451667516044605No Hit
TTCAGTAGGGGGAGGCAAGATGGAGTTAGTGCAGGTCCTGAAACGCGGGCTG1200.10365290098556634No Hit
AGACCTGGGGAAACATCTTTGAGTGACAGGCTCCCTTTGGCCACACAGAATA1200.10365290098556634No Hit
ACCAATGGGCTATCACCAGCAACAGAAGGTGCAGACTGTGTTGGTGGCGGTA1190.10278912681068661No Hit
TCTGTAGGGGAAGGGGAGTCCGAACTAGTCTCAGGCTTCAACATCGAATACG1190.10278912681068661No Hit
GGTGCCGGGCATCGATGGACATTTGAGTTTCTTCTGCCTGTTAGCTATTGTG1190.10278912681068661No Hit
TCTGGAGGACCTTTCTTGAAACATCCTATTACCTCCCCTGCTTCTTCTGATG1180.1019253526358069No Hit
CTTGCGCGGGAAGCACAGGCAACCAAAGCAAAAAGAACAAATGGGATCACTT1180.1019253526358069No Hit
ACTCGAGGGATGGTGGGAAGTTGAAAAGAGGCAGAACACAAAACAAAACAAA1180.1019253526358069No Hit
GCGTAAGGGATCCTTAGATTCTCCCACTGCCTAAGAATCATTGACTGCTATT1180.1019253526358069No Hit
GTTTGGGGGCAGGACTCTTTCTCTGACATCAAGACGCAGAGCTCGTCAATGG1180.1019253526358069No Hit
ACTGAAGGGGGGATGCCTGGTCCAGTGCAGAGGGCTGAGTATCAGTCATCCA1180.1019253526358069No Hit
CTCCTAGGGAAAGAGAGTAGCCTGGACTTAAAATATTTGAAATCAGCTTATG1180.1019253526358069No Hit
ATCAAAGGGAGGCCACACAGCAGGAGGTGAGCAGCAGGTGACCCAAGCAAAA1180.1019253526358069No Hit
ATTGCTGGGGAGGAGGCTGAGGTGGGAGGATTGCTTGAGCCCAGGAGGCAGA1180.1019253526358069No Hit
CACGCGGGCGGCAGTACTCAGTGGAAGGGTGATATTATGACACTGACACTGC1180.1019253526358069No Hit
ACTCGAGGGATCTTTAAAAAGTAGACTTTTCTTCTTCACCTTTGACTAAAGT1170.10106157846092717No Hit
GTACTAGGGACTTACAAATCAGGGTGATTTTTGGCTCTATTTTTATATGACT1170.10106157846092717No Hit
TTTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA1160.10019780428604746No Hit
GACAAGGGGGCCACTGGGGACATACAATCAGGCCAGATTCGGTAAATAAGAA1160.10019780428604746No Hit
CGAGCAGGGACAAAAATAAACTGGGGTTAATTATGAAGTTCTTGAGTGCTGG1160.10019780428604746No Hit
TGTAGAGGGATGGCTAGCTGCTCTTTGAAAATCACCCAAAGTTTCCACCATT1160.10019780428604746No Hit
CTTTCTGGGAATGCACTGACCTTTTGGTGTTCATTTTGATTGGTTAATGATT1160.10019780428604746No Hit
GCCGCTGGGCACATGATGGATAGCACAAACAAGCTCCCTTAGGCCCCTTTTA1160.10019780428604746No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)