FastQCFastQC Report
Sat 31 Dec 2022
SRR5846700.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846700.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114698
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGCTAAATATCTCGTATGC8150.7105616488517673No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCTAAATATCTCGTATGCCGTCT7130.6216324608973129No Hit
CTTCTTGGGGCAAAAACCAACTCCATTTTGCCAGGATTTTGTTGTGCTGAGA2060.17960208547664303No Hit
ACATCAGGGGTGGAAAAGCATTCCATAATTTCTCTTCTTTGCAAATACATGA1850.16129313501543183No Hit
GTTTTAGGGATTTAGATTAAATTTTAAATGCAAGAAACACTTTCGAAATTAC1840.16042128023156463No Hit
CTTTAAGGGCAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAAC1830.15954942544769743No Hit
AATGCTGGGATCCCCTTGCCGATGCCCACCTCACCAAGAAGCTACTGGACCT1800.15693386109609583No Hit
GAGGCGGGATTTCTTTTTTAGTTCAAAGGTGAAATTTTGACCTTTGTATTAA1780.15519015152836144No Hit
CCGAGTGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAAC1740.15170273239289264No Hit
ATAGGCGGTGCTTCTATTCAGCCTGAAGAAGTTATGAAAGATGGATCTTCAT1740.15170273239289264No Hit
TGCTGAGGGAGCCTTTTTAAGCTAATCAGATTGACACCTTAAAGTTTGATAA1720.14995902282515824No Hit
TTTATAGGGGAACATTAGTATGTTGGTGTAGGTTTCTCTTGGTTCCCAAATC1690.14734345847355665No Hit
TACTATGGGAGGTTGGCAAATAGCACTGTTTTCTTAGCTGCAAGAATTCATT1690.14734345847355665No Hit
TGATTGGGGAAGCAACAGGCTCACTGGTCACGGATTTGTGTCTGTGACTTTT1640.14298418455422063No Hit
TCGCCGGGGCATCACTATCGGAGAAAAACTTCATGTGCACAAAAGCACCAGT1640.14298418455422063No Hit
TACGCAGGGAGTTGAGAAAGAAGAGAAGAAGAAAATCAACCAGCAGGAAAGT1610.14036862020261906No Hit
AGACTTGGGATAAAACCAAATGTGTTTGAATCTTATAATTCTTCCAAGGCCT1610.14036862020261906No Hit
TGCGTTGGGATTCTAATTATTTTAACAAGTATCCCTTAGTTTTCATCTTTAA1590.13862491063488466No Hit
TAAAGCGGGTGAGAAGGCAGAAATATGCATGTTAAACAATTCCCCAACAAAA1580.13775305585101746No Hit
TTAGAGGGCACAGTAGAACGTGAAATTAAGTAGAGTCCCCTTTTAAACAAAT1570.13688120106715027No Hit
TTTGGCGGGGTGGTTCCTGTCTTTAGTTCTGTTTATGTGATGAGTCACATTT1570.13688120106715027No Hit
TAAAAAGGGAGGTGCTTCCCCTGAGGTGCAGGGGAAGACCAAAGTTTGCAAC1560.13600934628328307No Hit
GCGTAAGGGCAGCCCAGCCAACATGGCGAAACCATGTCTCTACTAAAAATAC1550.13513749149941587No Hit
TGTTTAGGGGATCTAATGCGGGGAAAGTACCTGTATCAAAGGAATTTTAACA1540.13426563671554867No Hit
GAGCTGGGAGAAGGCTTTTCTGTAATCCAGGTGAGAGGCGACAGTGGCGATG1530.13339378193168147No Hit
CGACTAGGGAGAGCATTTGTATCTACAATACATTTTAAAGTCAGAGTTCGTG1520.13252192714781424No Hit
GATGGGATGGCCGCCGTAGTCCAGCTCGGGCTGGTCGCCGATGCAGACGAAG1510.13165007236394705No Hit
TTTGTAGGGGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT1500.13077821758007985No Hit
TGTTTAGGGTGAGTTCGTGTGATTTTGAATGAGTTTTTTTGTTTCTTAATTT1490.12990636279621265No Hit
ATGCCAGGGCCACTCACACTTTCTCCGTATCAGCAGTAAAGGGCTGTTTCAG1480.12903450801234545No Hit
TAAAACGGGAGTTGATTTTAAGGCAAGAAATACAACTAGAGATAAAGTGCTC1470.12816265322847825No Hit
TTTGCAGGGAGACTAAGGTGTTAAATGACTTGTTCACAATCTTTCCACTACC1470.12816265322847825No Hit
AGACCAGGGAATACTTCCGAATTAAATTATTTAATATTTGACTGATTTCAAT1470.12816265322847825No Hit
TTACTGGGGCGTTTTACAGAAGTAGAAAGAGTGGTTTGGAGAGGTTAAGTAA1440.12554708887687668No Hit
CAACCTGGGGAAGAGTATGTACCAGTGTTTTGGTTACATAGTGGGAAAAATA1440.12554708887687668No Hit
CAACGCGGGACCTTTATCTTGGTCTTTGTTGATTTGGGTTTTGTTGGGGTTT1440.12554708887687668No Hit
CTATGGGGGGAAATATTAGCAAATTGAATCAAAAAATGTGTTAAAAGAATTT1440.12554708887687668No Hit
AAACTGGGGGAATGTGGGAAAGCCTTTAGTGATTTCTATTACTTTCGAAATC1420.12380337930914227No Hit
GAACAAGGGATTCATCACTAGTTCATTTTTCCCAACTCTCTATCAGATTTGG1420.12380337930914227No Hit
AGATGTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1420.12380337930914227No Hit
CTACAAGGGCAAAGCATTTGAGTGAAATTAGTCCACAAGAGATAGTAATAAA1420.12380337930914227No Hit
AAACAGGGGCAAACACTGAAAAGAGAACAATGATCTGATTTGCCAACGATTC1410.12293152452527507No Hit
TATAAAGGGATGTACAAGACTTATATCTACATTTTTTGATCACCAGTTGTAC1410.12293152452527507No Hit
TCTAGAGGGAAGATGTGCTTCTCTGCAATAACCAAAGACCTGAGCATGTCAC1400.12205966974140788No Hit
GCACTTGGGGTCCTTTCTATAAGCTTGTCACTGCAAAAAGTTGCCTTTTGCT1400.12205966974140788No Hit
GTGACTGGGAACGTTTCGAACGTTTTGCATCCAGCTGTGGTTAACACGGGAT1400.12205966974140788No Hit
TGTTGAGGGTGGGAGTGAAAAGTCATACAAAAAGCAAGTGGCTATGATTCTG1400.12205966974140788No Hit
ACTGAGGGAAATTCAACATCTTATATAGGTGCAGTTCCTGATGCTCCAAAAC1400.12205966974140788No Hit
ACCTGAGGGATATTAAAAATTTAGTGACTATGGTAAGCTTTTGTTCATTTGT1390.12118781495754068No Hit
CTTGCGCGGGAAGCACAGGCAACCAAAGCAAAAAGAACAAATGGGATCACTT1390.12118781495754068No Hit
GGTTAAGGGATGTGGGAATGTAAAATGGTGTAGCCGCATTGGAAACAGTTTC1390.12118781495754068No Hit
CAGCAGGGCTATATACTGACAATCTACAATCTAAAAAGGAAATGAAGAAAAC1390.12118781495754068No Hit
TGAACAGGGATACTAAAGTGAGATGCATTTTTTCTCATTTTCAGCAAGACTC1390.12118781495754068No Hit
CGAGCAGGGACAAAAATAAACTGGGGTTAATTATGAAGTTCTTGAGTGCTGG1380.12031596017367348No Hit
TTGCTCGGGGCGATCTCAATGCGGGCTACTGAAAAATTTACATTTTGAGTCA1380.12031596017367348No Hit
AGGACTGGGACTTTGTAACCAGGGAGGTGATGCAGCGTGACATAGCAGCCGG1380.12031596017367348No Hit
GAGCAAGGGGATCTTACAGCCTACAGAATGCTCTCTGGCTCCCGGCTTTGCC1380.12031596017367348No Hit
GACTAAGGGCAAAACTTGTATGATACAGCCATAGGATTTCTCAGAAGGGAAT1380.12031596017367348No Hit
ACGATTGGGCTGACTAAAACTCTCATTCAATAAAATTATAGTCAAATCTTGC1370.11944410538980628No Hit
CAAGCGGGCACATTGGCCTAAAACCATCAGGCAATTTAAATATGAGATCCCT1370.11944410538980628No Hit
AATACTGGGAGGAAGGAATATGTGGAGAGGGCCATTGAATATCGACTTCATG1370.11944410538980628No Hit
ATCCGAGGGGTGTTACCAGTGGATTTGAGGAATTAAAATGTCACAGTTTCAT1370.11944410538980628No Hit
TTGCATGGGATGGACATAATAGTTCACTTACAAATGTGATTTGATGTTAACA1360.11857225060593907No Hit
TCTGATGGGGGGTGCAGGGGAACCCCAGTCCCCGGACGAATTGCTCCCGAAA1350.11770039582207187No Hit
GATGCTGGGATGGGGGTCCTTGGCAATATATAAAATTTATAGTAAGAGTTTC1350.11770039582207187No Hit
TCTGATGGGGTAACAATTTCTTGCTTATACTAACTGATGAGTGTCTCACATT1350.11770039582207187No Hit
CAAAAAGGGAGAGAGCATCTGCAACAAAAAGTCATTTTTCTGGAGTGGAAAA1350.11770039582207187No Hit
TAACAAGGGAGAAATATTACACTCTACAAAATTGTTTTATCAAGGTCCAAAA1340.11682854103820467No Hit
GGTGCCGGGCATCGATGGACATTTGAGTTTCTTCTGCCTGTTAGCTATTGTG1330.11595668625433747No Hit
GCGTAAGGGATCCTTAGATTCTCCCACTGCCTAAGAATCATTGACTGCTATT1320.11508483147047027No Hit
TCGTAAGGGATTAAAAAACTCTTCTTACATATAAAAAGCATATATTAATGTT1320.11508483147047027No Hit
GTGTTAGGGAAGAAGCTGGGATTGCAGGCACCAACCACCATGCCTGGCTATT1320.11508483147047027No Hit
TGATAAGGGTATCTATATGTAAGTTACTAACATTATTATTTTGTCAGATGGA1320.11508483147047027No Hit
GGGCAGGGGCGCACCAACCTAACTGTCTTAAAGTTTAAATACATGATACTTG1310.11421297668660307No Hit
ACCAATGGGCTATCACCAGCAACAGAAGGTGCAGACTGTGTTGGTGGCGGTA1310.11421297668660307No Hit
GTTTGGGGGCAGGACTCTTTCTCTGACATCAAGACGCAGAGCTCGTCAATGG1310.11421297668660307No Hit
GTCCAAGGGAAGTTGAAAGTTCTACAAATCTCTAGTGCAGGGGCCAAATGCT1310.11421297668660307No Hit
ACTCGAGGGATCTTTAAAAAGTAGACTTTTCTTCTTCACCTTTGACTAAAGT1310.11421297668660307No Hit
TCTCCGGGGGGACAAGAGACTAAGTTCTAGTCAAAGGAACACACACGGAAGT1310.11421297668660307No Hit
GGCATAGGGATGTGCTTACATGTTTAATATCTGCCTCACTTTTGAATGAAAG1300.11334112190273588No Hit
GCTTACGGGAGTAGAAGAAATAGCAACTGCTTTGATATGGAGATTTACCCCT1300.11334112190273588No Hit
TTAACTGGGGCTCTAGAAACACTAAGATGAAAAGATAGAGCCCCTGCCATTG1300.11334112190273588No Hit
GCATGAGGGCATGTTCACTGGTGGACCAGTTGGCATTGACCTAAACGGACAG1290.11246926711886868No Hit
AGTTCAGGGAATGGAAGATGTGCTTGGTGAATATTATGCATGTTCTTGAAGA1290.11246926711886868No Hit
AAACCAGGGACTTCGAGACTATGTGGATCATTCTGAGACTCTGCAGAAGTTG1280.11159741233500149No Hit
CTAAATGGGTATCACATTGGTCTTTGTCTTGCAACAGTTTTGTAGGATGAGT1280.11159741233500149No Hit
ATTCATGGGTCCGATCTTACCCTGGGAAGAAAGTCATATTTTTGCATTTCAG1280.11159741233500149No Hit
GTTGTTGGGGAATGGATAAAGAAATTGTGGTGTCTCTATTCACAATGGAGTA1280.11159741233500149No Hit
TATTTGGGGATGTGATTGTTTATTAGTAACAGTTTATGAATACCATAAGAAA1270.1107255575511343No Hit
TTCAGGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1270.1107255575511343No Hit
GACGTTGGGACCAGGAGATTTCATCTCTAAGTGTACAATTCAGTGATATTTT1270.1107255575511343No Hit
TTAGGTGGGAATGGAATATATTATTTATGTGAATTGTGATGGAGTGTGCCTT1260.1098537027672671No Hit
ACAAGCATTGGGAGGATTCGATCTGCTAAGTTTGTTGGAAAAGATCTGGCAG1260.1098537027672671No Hit
TGCTGGGGAGATAGACTGAGTCTTCTCTCGTAGGTTACCATTACATAGTAAT1260.1098537027672671No Hit
GCTCACGGGAGGATGATGTAATTAAAAAATGGTCAGTGTGCAAAGAAAAGAA1250.1089818479833999No Hit
GTATACGGGATATTAAAAATTGGAGAATAAGTGTGCATCCATTGATATTTGG1240.1081099931995327No Hit
TGGATTGGGACTCAATTCTACCATAATTATGTATATGAGAAAAATATTTTTA1240.1081099931995327No Hit
GGCCTCACTCCGTAGGTCTTATGTTGAAGTCTTGTTTTACTTGTATGCACTT1240.1081099931995327No Hit
ACTCCTGGGATTCAGTAAAGTTTCAGGATGCAAAATTAATGTACAGAAGTCA1230.10723813841566548No Hit
GATCCGGGGCGCTCAGGCTAGGGCTCAGCAGCTGCTTGTGGGCTCAAAAGCA1230.10723813841566548No Hit
CGTATAGGGCTGAGATCTGGAGTCACGTATGTGTGTTAGAGTTCCCTCTTCA1230.10723813841566548No Hit
GTACTAGGGACTTACAAATCAGGGTGATTTTTGGCTCTATTTTTATATGACT1230.10723813841566548No Hit
AGTATCGGGTGAATGGAAGAGAGACAAAGCTATCAGCTATAGATCATTGTTT1230.10723813841566548No Hit
ACTCGAGGGATGGTGGGAAGTTGAAAAGAGGCAGAACACAAAACAAAACAAA1220.10636628363179829No Hit
CACAATGGGATGGGAGGGAATCTGGGAGATAAAGTTACTGGGAAGTGAAGAT1220.10636628363179829No Hit
TGTAACGGGAACGTAATTTTAATAGCTTAAGATTTTAAGAGAAAATATGAAG1220.10636628363179829No Hit
AGACCTGGGGAAACATCTTTGAGTGACAGGCTCCCTTTGGCCACACAGAATA1220.10636628363179829No Hit
ACAAGGGATCTGAAAGCACAAGGTGATACTCATTTTTATGGTCTTCCCATTT1220.10636628363179829No Hit
TACAACGGGATACTGGGCTAACAATGAGATAAAATAAAAATACCTTTGAATA1220.10636628363179829No Hit
AACCGGGGATTGCACGTGAAATGGGATGGTACGTTTTATGATAGTGGATCAG1220.10636628363179829No Hit
AAACAGGGGTATGGCTGGGCATCTTAAAAAGAAATTTTCTTTGGCTAGCCTC1210.10549442884793109No Hit
AACTAGGGGACCCGCTAATTACAATATTAGTCATTGCACTAGAATGTATTCC1210.10549442884793109No Hit
AGTTATGGGCATAAAATACAAGTTTTTAGATGTTTATGCTTTGCCTTTCTTT1210.10549442884793109No Hit
ACAAAAGGGAGTGCCGAGGGCTTTTGAGTGAAATGCCAGTCATGAAGGTGCT1210.10549442884793109No Hit
CACGCGGGCGGCAGTACTCAGTGGAAGGGTGATATTATGACACTGACACTGC1210.10549442884793109No Hit
TGTAGGGTCTACGTGCGGGTGACAAATGTTCTTCCAAAGAACATTGAATACA1200.10462257406406389No Hit
CTTCTTGGGAGGGGTGCTAAGTCATCGTAGGGACCATTAGGGGTAGTATAAT1200.10462257406406389No Hit
CTGCAAGGGCATGTTTATCCAGTGGCATGTTATGATTTTTTAGTTTCTCATA1200.10462257406406389No Hit
TATGCCGGGGGGACTGAAAATAAAAGGTTTTTAGTAGCTTAATTAGAAACTA1200.10462257406406389No Hit
AAAAGACAAGAATCAGAGCCCTGTCTTCATTATCTATATCCCCAGAGCCTGG1190.10375071928019669No Hit
CTTTGCTCGGGTCTGTAGCAGCAGCATGACTTGAGCACCAGCTCTGAGCCAC1190.10375071928019669No Hit
ATATCCGGGCTTGACGTGGGGGGTTAATTCATGATGTTTGCCCTTTTAATAA1190.10375071928019669No Hit
TGGCGGGTGTGGTAGACTGATTTGTACTCCTTTGGGTATATAACCAGTAATG1190.10375071928019669No Hit
TCTGGAGGACCTTTCTTGAAACATCCTATTACCTCCCCTGCTTCTTCTGATG1180.10287886449632949No Hit
AGTCCAGGGTTAATACTTCAGAGCTAATTCAAGAAACCTGTGTGCATTAACG1180.10287886449632949No Hit
CAATGCGGGATAGAATACATTTACAGGAATGTTTATTGCAACATTGTTTAAA1180.10287886449632949No Hit
GTTTGTAATTATTTTCCAACAATAAATAAATTGAATTTATTTCATTAGATAT1180.10287886449632949No Hit
GCTTACGGGAAACTGCCAAAATGTGAATATGCAAAGCCTTTCTGAATCTATA1170.1020070097124623No Hit
GACCAGGGGCTGACTGAATTTTAAGGCATTTGGGTAGCACATATGACAACTG1170.1020070097124623No Hit
AGAACAGGGACAGTAGTCATTGAAAGTTATGTTTCTTTGTTTACTTCATTTT1170.1020070097124623No Hit
CTCCTAGGGAAAGAGAGTAGCCTGGACTTAAAATATTTGAAATCAGCTTATG1170.1020070097124623No Hit
GATATAGGGGGTCCATCTGCCCTCCATAGAAAAATGTCTCTGGCTCATAAAA1170.1020070097124623No Hit
ATAGCCGGGACTTTTCTTTGTGAAAATAAAAATGAAAAGATTTAAAAAGAAG1170.1020070097124623No Hit
TGCAAAGGGTATGTAAAAATGAAGGTGGCACCGTGGTGAGACCTAATGAGAA1170.1020070097124623No Hit
CTCATAGGGCAGTGAGTGGAAAATGAAGTTCGTTTTCTCTTAGGAAAATGGG1160.1011351549285951No Hit
GGTGCTGGGGGCAGTAGCAGGACCGCTGCTGTGGAGCTGGTCGCAGGCGGTG1160.1011351549285951No Hit
GCCGCTGGGCACATGATGGATAGCACAAACAAGCTCCCTTAGGCCCCTTTTA1160.1011351549285951No Hit
TCAGTAGGGCATTATTGAGAGTCTCTAAAGTACTGCATAAATACTTTGGTCT1160.1011351549285951No Hit
ATAACTGGGAAATCATTAAAATTAAAAATCATTCTTCAATATTTAAAATGTG1150.10026330014472788No Hit
GAATGGGGCTCTCAAAGCATTTTAACTCAGTTGCTTTAGGGTCCATTTTTTT1150.10026330014472788No Hit
TTGGGTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1150.10026330014472788No Hit
GTTTATGGGGTGCTTCGAGAGTGTATACATGTCCCATTTCAGATTTATATAT1150.10026330014472788No Hit
CGAACAGGGTGCTCCAGTGCTGATGGAGGCCAAAGACAACCCCAGGGTTTTC1150.10026330014472788No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)