FastQCFastQC Report
Sat 31 Dec 2022
SRR5846699.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846699.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116363
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGCTAAATATCTCGTATGCCGTCT7350.6316440793035587No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGCTAAATATCTCGTATGC7060.6067220680113095No Hit
ACATCAGGGGTGGAAAAGCATTCCATAATTTCTCTTCTTTGCAAATACATGA2040.17531345874547752No Hit
GAGGCGGGATTTCTTTTTTAGTTCAAAGGTGAAATTTTGACCTTTGTATTAA2010.1727353196462793No Hit
GCTCACGGGAGGATGATGTAATTAAAAAATGGTCAGTGTGCAAAGAAAAGAA1990.17101656024681386No Hit
CTTCTTGGGGCAAAAACCAACTCCATTTTGCCAGGATTTTGTTGTGCTGAGA1750.15039144745322827No Hit
AAACTGGGGGAATGTGGGAAAGCCTTTAGTGATTTCTATTACTTTCGAAATC1660.14265703015563366No Hit
AGACCAGGGAATACTTCCGAATTAAATTATTTAATATTTGACTGATTTCAAT1650.14179765045590093No Hit
TGCGTTGGGATTCTAATTATTTTAACAAGTATCCCTTAGTTTTCATCTTTAA1650.14179765045590093No Hit
CCGAGTGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAAC1640.14093827075616822No Hit
GATGGGATGGCCGCCGTAGTCCAGCTCGGGCTGGTCGCCGATGCAGACGAAG1620.1392195113567027No Hit
CAACGCGGGACCTTTATCTTGGTCTTTGTTGATTTGGGTTTTGTTGGGGTTT1600.13750075195723727No Hit
TAAAAAGGGAGGTGCTTCCCCTGAGGTGCAGGGGAAGACCAAAGTTTGCAAC1590.13664137225750453No Hit
TTTGTAGGGGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT1580.1357819925577718No Hit
TATAAAGGGATGTACAAGACTTATATCTACATTTTTTGATCACCAGTTGTAC1570.13492261285803908No Hit
ACCTGAGGGATATTAAAAATTTAGTGACTATGGTAAGCTTTTGTTCATTTGT1560.13406323315830634No Hit
TTTGGCGGGGTGGTTCCTGTCTTTAGTTCTGTTTATGTGATGAGTCACATTT1560.13406323315830634No Hit
CTATGGGGGGAAATATTAGCAAATTGAATCAAAAAATGTGTTAAAAGAATTT1560.13406323315830634No Hit
GGTTAAGGGATGTGGGAATGTAAAATGGTGTAGCCGCATTGGAAACAGTTTC1550.1332038534585736No Hit
GTCCAAGGGAAGTTGAAAGTTCTACAAATCTCTAGTGCAGGGGCCAAATGCT1550.1332038534585736No Hit
GTTTGTAATTATTTTCCAACAATAAATAAATTGAATTTATTTCATTAGATAT1550.1332038534585736No Hit
GAGCAAGGGGATCTTACAGCCTACAGAATGCTCTCTGGCTCCCGGCTTTGCC1540.13234447375884087No Hit
TCGCCGGGGCATCACTATCGGAGAAAAACTTCATGTGCACAAAAGCACCAGT1540.13234447375884087No Hit
ATGCCAGGGCCACTCACACTTTCTCCGTATCAGCAGTAAAGGGCTGTTTCAG1530.13148509405910813No Hit
GAACAAGGGATTCATCACTAGTTCATTTTTCCCAACTCTCTATCAGATTTGG1530.13148509405910813No Hit
TACGCAGGGAGTTGAGAAAGAAGAGAAGAAGAAAATCAACCAGCAGGAAAGT1530.13148509405910813No Hit
GGGCAGGGGCGCACCAACCTAACTGTCTTAAAGTTTAAATACATGATACTTG1520.1306257143593754No Hit
TACTATGGGAGGTTGGCAAATAGCACTGTTTTCTTAGCTGCAAGAATTCATT1520.1306257143593754No Hit
GGCCTCACTCCGTAGGTCTTATGTTGAAGTCTTGTTTTACTTGTATGCACTT1520.1306257143593754No Hit
TAAAGCGGGTGAGAAGGCAGAAATATGCATGTTAAACAATTCCCCAACAAAA1500.12890695495990995No Hit
TGTTTAGGGTGAGTTCGTGTGATTTTGAATGAGTTTTTTTGTTTCTTAATTT1490.1280475752601772No Hit
ATAGGCGGTGCTTCTATTCAGCCTGAAGAAGTTATGAAAGATGGATCTTCAT1480.12718819556044447No Hit
ACAAGGGATCTGAAAGCACAAGGTGATACTCATTTTTATGGTCTTCCCATTT1480.12718819556044447No Hit
AGACTTGGGATAAAACCAAATGTGTTTGAATCTTATAATTCTTCCAAGGCCT1480.12718819556044447No Hit
TGTAGGGTCTACGTGCGGGTGACAAATGTTCTTCCAAAGAACATTGAATACA1470.12632881586071174No Hit
GCACTTGGGGTCCTTTCTATAAGCTTGTCACTGCAAAAAGTTGCCTTTTGCT1460.125469436160979No Hit
GTTGTTGGGGAATGGATAAAGAAATTGTGGTGTCTCTATTCACAATGGAGTA1460.125469436160979No Hit
GTACTAGGGACTTACAAATCAGGGTGATTTTTGGCTCTATTTTTATATGACT1460.125469436160979No Hit
TTAGAGGGCACAGTAGAACGTGAAATTAAGTAGAGTCCCCTTTTAAACAAAT1450.12461005646124626No Hit
GTTTTAGGGATTTAGATTAAATTTTAAATGCAAGAAACACTTTCGAAATTAC1450.12461005646124626No Hit
TGTTTAGGGGATCTAATGCGGGGAAAGTACCTGTATCAAAGGAATTTTAACA1450.12461005646124626No Hit
GTATACGGGATATTAAAAATTGGAGAATAAGTGTGCATCCATTGATATTTGG1450.12461005646124626No Hit
TGATTGGGGAAGCAACAGGCTCACTGGTCACGGATTTGTGTCTGTGACTTTT1450.12461005646124626No Hit
AGTACCGGGATCTTTCAAAAGAAAAAATTCATCTAGACAACACTTCATTTCT1450.12461005646124626No Hit
TGAACAGGGATACTAAAGTGAGATGCATTTTTTCTCATTTTCAGCAAGACTC1450.12461005646124626No Hit
GCTCATGGGCAGGAAATCAAATCAGTCTTCCGGTCTGCAGCACGGAAAAGCA1440.12375067676151355No Hit
TAACAAGGGAGAAATATTACACTCTACAAAATTGTTTTATCAAGGTCCAAAA1440.12375067676151355No Hit
GCGTAAGGGCAGCCCAGCCAACATGGCGAAACCATGTCTCTACTAAAAATAC1440.12375067676151355No Hit
TCTCCGGGGGGACAAGAGACTAAGTTCTAGTCAAAGGAACACACACGGAAGT1440.12375067676151355No Hit
TGGATTGGGACTCAATTCTACCATAATTATGTATATGAGAAAAATATTTTTA1430.12289129706178081No Hit
ATCGCTGGGGATGTATTATTAGTGACAGCATTTGATTATTGTCTAATTAAAA1420.12203191736204808No Hit
CTTCTTGGGAGGGGTGCTAAGTCATCGTAGGGACCATTAGGGGTAGTATAAT1420.12203191736204808No Hit
TTTATAGGGGAACATTAGTATGTTGGTGTAGGTTTCTCTTGGTTCCCAAATC1410.12117253766231534No Hit
TCTAGAGGGAAGATGTGCTTCTCTGCAATAACCAAAGACCTGAGCATGTCAC1400.12031315796258261No Hit
CGAGCAGGGACAAAAATAAACTGGGGTTAATTATGAAGTTCTTGAGTGCTGG1400.12031315796258261No Hit
TACAACGGGATACTGGGCTAACAATGAGATAAAATAAAAATACCTTTGAATA1400.12031315796258261No Hit
TCTGATGGGGTAACAATTTCTTGCTTATACTAACTGATGAGTGTCTCACATT1400.12031315796258261No Hit
TATAACGGGCAATGAATAATTTCCAAATAATTTCATTAGATAATTGAGGCTT1400.12031315796258261No Hit
AACCGGGGATTGCACGTGAAATGGGATGGTACGTTTTATGATAGTGGATCAG1390.11945377826284988No Hit
CTACAAGGGCAAAGCATTTGAGTGAAATTAGTCCACAAGAGATAGTAATAAA1390.11945377826284988No Hit
CAAGCGGGCACATTGGCCTAAAACCATCAGGCAATTTAAATATGAGATCCCT1390.11945377826284988No Hit
GGCATAGGGATGTGCTTACATGTTTAATATCTGCCTCACTTTTGAATGAAAG1380.11859439856311714No Hit
GTTCACGGGAAGGAATATTGGCACTCATTTTAGGTAACAATATTTTGTTACC1380.11859439856311714No Hit
ACTGCGGGCAGTGCTGGACTAGATATGCCATTGGATAAAAGGCAGCATCTCT1380.11859439856311714No Hit
CAATGCGGGATAGAATACATTTACAGGAATGTTTATTGCAACATTGTTTAAA1380.11859439856311714No Hit
TTTGCAGGGAGACTAAGGTGTTAAATGACTTGTTCACAATCTTTCCACTACC1360.11687563916365168No Hit
GAGCTGGGAGAAGGCTTTTCTGTAATCCAGGTGAGAGGCGACAGTGGCGATG1360.11687563916365168No Hit
AGGACTGGGACTTTGTAACCAGGGAGGTGATGCAGCGTGACATAGCAGCCGG1360.11687563916365168No Hit
TTACTGGGGCGTTTTACAGAAGTAGAAAGAGTGGTTTGGAGAGGTTAAGTAA1350.11601625946391894No Hit
AATGCTGGGCAACATGGGCAAATTAACTTGCTGCTGAGTTTTCTGAACAGTG1350.11601625946391894No Hit
TGCTGAGGGAGCCTTTTTAAGCTAATCAGATTGACACCTTAAAGTTTGATAA1340.1151568797641862No Hit
AAACAGGGGTATGGCTGGGCATCTTAAAAAGAAATTTTCTTTGGCTAGCCTC1330.11429750006445348No Hit
CTTGCGCGGGAAGCACAGGCAACCAAAGCAAAAAGAACAAATGGGATCACTT1330.11429750006445348No Hit
CGACTAGGGAGAGCATTTGTATCTACAATACATTTTAAAGTCAGAGTTCGTG1330.11429750006445348No Hit
ACTCGAGGGATCTTTAAAAAGTAGACTTTTCTTCTTCACCTTTGACTAAAGT1330.11429750006445348No Hit
GCTTACGGGAAACTGCCAAAATGTGAATATGCAAAGCCTTTCTGAATCTATA1320.11343812036472074No Hit
TTGTCAGGAAGTTAGCAATCGGTTCTTCCAAACTCCTGTGGGTTGAATATGG1320.11343812036472074No Hit
AATACTGGGAGGAAGGAATATGTGGAGAGGGCCATTGAATATCGACTTCATG1320.11343812036472074No Hit
TCTGGAGGACCTTTCTTGAAACATCCTATTACCTCCCCTGCTTCTTCTGATG1310.112578740664988No Hit
GATGCTGGGATGGGGGTCCTTGGCAATATATAAAATTTATAGTAAGAGTTTC1300.11171936096525528No Hit
TGCAAAGGGTATGTAAAAATGAAGGTGGCACCGTGGTGAGACCTAATGAGAA1300.11171936096525528No Hit
CAAAAAGGGAGAGAGCATCTGCAACAAAAAGTCATTTTTCTGGAGTGGAAAA1300.11171936096525528No Hit
TTGTTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1280.11000060156578981No Hit
ACGACGGGGTCATCCTCCCAGGAACCTATTAGTAGAAGATATGGTTTCTATT1280.11000060156578981No Hit
GACTAAGGGCAAAACTTGTATGATACAGCCATAGGATTTCTCAGAAGGGAAT1280.11000060156578981No Hit
AGACCTGGGGAAACATCTTTGAGTGACAGGCTCCCTTTGGCCACACAGAATA1270.10914122186605707No Hit
GACGTTGGGACCAGGAGATTTCATCTCTAAGTGTACAATTCAGTGATATTTT1270.10914122186605707No Hit
ATATGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1270.10914122186605707No Hit
TATGCCGGGGGGACTGAAAATAAAAGGTTTTTAGTAGCTTAATTAGAAACTA1270.10914122186605707No Hit
AGATGTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1260.10828184216632436No Hit
TTCAGGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1260.10828184216632436No Hit
CCAACAGGGTATGCTTTGCAGAAAAGATTCTTTGTATTGGAAATTAATAAGA1260.10828184216632436No Hit
TCTAATGGGGTGGACCTGATAGATATCTGCAGAACTCTCCACCCCAGAACAG1260.10828184216632436No Hit
GTTTATGGGGTGCTTCGAGAGTGTATACATGTCCCATTTCAGATTTATATAT1260.10828184216632436No Hit
AGCACTGGGATTCTTTTAAAATTTCCTCTGATACTATAAGCTTTTTGCATTT1260.10828184216632436No Hit
AGTATCGGGTGAATGGAAGAGAGACAAAGCTATCAGCTATAGATCATTGTTT1260.10828184216632436No Hit
CTTTAAGGGCAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAAC1250.10742246246659161No Hit
GCTTTAGGGATGGTTGAACACTGGTAACTAAAACTATGGAAAATGAAACTGT1250.10742246246659161No Hit
GCTTACGGGAGTAGAAGAAATAGCAACTGCTTTGATATGGAGATTTACCCCT1250.10742246246659161No Hit
CTGCAAGGGCATGTTTATCCAGTGGCATGTTATGATTTTTTAGTTTCTCATA1250.10742246246659161No Hit
ACCAATGGGCTATCACCAGCAACAGAAGGTGCAGACTGTGTTGGTGGCGGTA1240.10656308276685887No Hit
AACGCAGGGTAGGCATTCAAGGCTATAAAGTGCCCACTAATGGTTTTAGCTG1240.10656308276685887No Hit
CACGCGGGCGGCAGTACTCAGTGGAAGGGTGATATTATGACACTGACACTGC1240.10656308276685887No Hit
TAGGGGGAACTTCTATAAGGCTATTAACAGATTTCTCAGCAGAGACCTCGCA1230.10570370306712616No Hit
GTTTCGGGGGGATTGCTTTTAGCTGCAAGTAATAGAACATTTAGTCGAAAGT1230.10570370306712616No Hit
TTAAAGCGGGAGATCAGATTAGAAAAAAGAAAATCAAAACAAAAAAGACAAT1230.10570370306712616No Hit
TAAGTAGGGGGGTCATTGACTTTAAAGTATAAAATTATAAAACTTTAAAAGA1220.10484432336739342No Hit
CGGCTTGGGATTCCTTACCACTGTCATAAAGTAGCTTCCAATCTGTTTGAGA1220.10484432336739342No Hit
AATCATGGGCCTACAGCTGGTTTTACACACACACGTTAAGAGTGATATAAAT1210.10398494366766067No Hit
TTGGGTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1210.10398494366766067No Hit
AGTTATGGGCATAAAATACAAGTTTTTAGATGTTTATGCTTTGCCTTTCTTT1210.10398494366766067No Hit
AAACAGGGGCAAACACTGAAAAGAGAACAATGATCTGATTTGCCAACGATTC1210.10398494366766067No Hit
TGGACTGGGACACACATACATACGCACACATGCTTTGGGTCCTTCCATAATA1210.10398494366766067No Hit
ACAAGCATTGGGAGGATTCGATCTGCTAAGTTTGTTGGAAAAGATCTGGCAG1210.10398494366766067No Hit
TTAACTGGGGCTCTAGAAACACTAAGATGAAAAGATAGAGCCCCTGCCATTG1210.10398494366766067No Hit
CTTTCTGGGAATGCACTGACCTTTTGGTGTTCATTTTGATTGGTTAATGATT1200.10312556396792794No Hit
TCTGATGGGGGGTGCAGGGGAACCCCAGTCCCCGGACGAATTGCTCCCGAAA1200.10312556396792794No Hit
GCCGCTGGGCACATGATGGATAGCACAAACAAGCTCCCTTAGGCCCCTTTTA1200.10312556396792794No Hit
CAACCTGGGGAAGAGTATGTACCAGTGTTTTGGTTACATAGTGGGAAAAATA1200.10312556396792794No Hit
ACTGAGGGAAATTCAACATCTTATATAGGTGCAGTTCCTGATGCTCCAAAAC1200.10312556396792794No Hit
TGATAAGGGTATCTATATGTAAGTTACTAACATTATTATTTTGTCAGATGGA1200.10312556396792794No Hit
ACGATTGGGCTGACTAAAACTCTCATTCAATAAAATTATAGTCAAATCTTGC1190.10226618426819523No Hit
CTATCGAGGGACAATAGCAGAGACATGGAATCAACCTAGATGCCCATCAACA1190.10226618426819523No Hit
TACACGGGAAGGAAACTAATGGAAGAGTGTAAAAGACTTCAGGGAGAAATGA1190.10226618426819523No Hit
TCGTAAGGGATTAAAAAACTCTTCTTACATATAAAAAGCATATATTAATGTT1190.10226618426819523No Hit
TAGTGTGGGCTACTTTAAAAGTTTTAATGGATTGCACCTCTGTAAACTATTC1180.10140680456846249No Hit
TTGCATGGGATGGACATAATAGTTCACTTACAAATGTGATTTGATGTTAACA1180.10140680456846249No Hit
GTGACTGGGAACGTTTCGAACGTTTTGCATCCAGCTGTGGTTAACACGGGAT1180.10140680456846249No Hit
ATGCAGGGAACAAATTATTAAAGCAGAAACTCTATCACTTCTTGGAACTAAA1170.10054742486872975No Hit
ACTCCTGGGATTCAGTAAAGTTTCAGGATGCAAAATTAATGTACAGAAGTCA1170.10054742486872975No Hit
GGTGCTGGGGGCAGTAGCAGGACCGCTGCTGTGGAGCTGGTCGCAGGCGGTG1170.10054742486872975No Hit
TAGTAAGGGGATGGCTTTTTCAGTTATACTTTTCGCTGATAGAGCATATATA1170.10054742486872975No Hit
ATATCCGGGCTTGACGTGGGGGGTTAATTCATGATGTTTGCCCTTTTAATAA1170.10054742486872975No Hit
CGACAAGGGCAGCAGTGAGAGAACATTTTATATAAATTACACTGTTGGGGAG1170.10054742486872975No Hit
GATAATGGGATAGAATGGGTTGGGAAGTGTTTCCTGTTTTTGGAAGAGTCTG1170.10054742486872975No Hit
GTATCAGGGAGTACTGTTAAGCTGGTGTGCTTTTCAAACAGTAATCAGGAAA1170.10054742486872975No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)