FastQCFastQC Report
Sat 31 Dec 2022
SRR5846664.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846664.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76187
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTCGTATGC27423.599039206163781No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTCGTATGCCGTCT18232.3927966713481306No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTCGTATGCCTGTC6670.8754774436583669No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTA4280.561775631013165No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAA3600.4725215587961201No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAAT1590.20869702180161973No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTCGTATGCC1430.18769606363290325No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAAACTCGTATGC1310.17194534500636593No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTCGT1270.1666951054641868No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCCTGTCTCTTATAC980.12863086878338825No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGG970.12731830889784346No Hit
AAAGCCGGGATGAGTTAATGAGTGTGGTTCAGAGGTTCCTTAGAGAGTGAGG850.11156759027130612No Hit
GCGCCAGGGAAGGCCCCCTTATTGGAAATAATCATGCTGAGAAAGGAAGAAT850.11156759027130612No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCCTGTCTCT810.10631735072912701No Hit
TACGTAGGGAGTCTAGGAAGGAATTTGGAAAAAAAATTTCTCATTCATCAGC800.10500479084358223No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)