FastQCFastQC Report
Sat 31 Dec 2022
SRR5846663.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846663.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76662
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTCGTATGC26233.4215126138112755No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTCGTATGCCGTCT17692.3075317628029532No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTCGTATGCCTGTC6590.8596175419373353No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTA4550.5935143878323028No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAA3660.4774203647178524No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAAT1820.23740575513292111No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAAACTCGTATGC1630.21262163783882498No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTCGTATGCC1300.16957553938065795No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTCGT1230.16044454879862252No Hit
AAAGCCGGGATGAGTTAATGAGTGTGGTTCAGAGGTTCCTTAGAGAGTGAGG910.11870287756646056No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGG880.11478959588844537No Hit
ATTCTCGGGCGATATCAACTTCAACTAAAATGGTTAATGGCAGAAAAATCAC840.10957188698442515No Hit
AACTAAGGGATTGCTAAAAACAGTTTCCAAGTGCCAAGGAACTTCAGGGTTC790.10304975085439984No Hit
TGTTCAGGGAAGGAAAGGAAAAAAATCTAATTTGGATGTACTTCACTACATT780.10174532362839477No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)