FastQCFastQC Report
Sat 31 Dec 2022
SRR5846662.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846662.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52582
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTCGTATGC33656.39952835571108No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTCGTATGCCGTCT21254.041306911110266No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTCGTATGCCTGTC8951.702103381385265No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTA6601.255182381803659No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAA6101.160092807424594No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAAT2840.5401087824730896No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAAACTCGTATGC1600.30428663801300826No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTCGT1450.27575976569928873No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGG1380.2624472252862196No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTCGTATGCC1280.2434293104104066No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCCTGTCTCTTATAC1220.23201856148491878No Hit
TTGAACGGGCTCTGCATCTGTTCTCTGAGGAACTCAAGTTTGGTTGCCAGAA1160.220607812559431No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCCTGTCTCT1080.20539348065878055No Hit
TGTGGATGGGAAGGCATGATTTTTGGATTGTTTTCTTTCCACTTGTTGGCGG1030.19588452322087407No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTC1030.19588452322087407No Hit
GGACCGGGGGAGAAGCAACTTCTAATACAAAGATGACTTATAACACATGAAA970.18447377429538625No Hit
TGTCTAGGGGACCGACCCTCGACTTCGGAGAGGCAGCGCGGTTCCTCTGGGT960.18257198280780496No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACC950.18067019132022363No Hit
TGCCGTGGGGGCAAGTTGCTTTGGCAGATATCATTCTCATTAATAAAACAGA930.17686660834506104No Hit
GATCACGGGCTGGGGACTTGTTTTGGTTTGGGGGCAGCCATCCTCCACCAGA920.17496481685747975No Hit
AAAAATGGGACCACCTTGAAGGGCCTTTTGCTTTCTGTGTTACCGTTTGCTG910.17306302536989845No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTC900.17116123388231716No Hit
TGGACTGACATCAGTTTGGGTGAGAGAGGGAGTCACTTTACTGAAAAGGCAG900.17116123388231716No Hit
GTAAGCGGGATATTTAATGAAATTGGTGGTTTTAGGAAGTCAACTTTAGTTT890.16925944239473584No Hit
GTTGCTGGGATGAAGTCATGAAGACCACCAAGATCATCATTGGCTGCTTTGT890.16925944239473584No Hit
TTTGACAGGGCAATTGCAAGCAAATAGATTTTTCTTTGTAACAATTTGTTCT870.16545585941957322No Hit
CTCGCGGGGCTGTGGGAAGAACATAGCTGGAGCACAAGCATCAACAGCCAGC860.16355406793199193No Hit
GGCTTGGGGCAATATCATGTGTAGATATTATGTATAGAAAATAAAATAAATT830.15784869346924804No Hit
GTCAAGGGGAGATGTTTTTCAGAGGCCTACAAATACAAGTATCTTTCTTATA830.15784869346924804No Hit
CCTTCTGGGGTTCATTTGAAGCCATTTCTGTAAACATCTTCCAAGATAGAAC830.15784869346924804No Hit
ACGTGCGGGACCGACTAATCACCACCCAACAATGACTAATCAAACTAACCTC830.15784869346924804No Hit
TCTTCAGGGCACTGAAACTGCCTTATTCCTAGACTGAACTATTATACATGAA810.15404511049408542No Hit
GGTATGAGGGGTCATAACAGTCTTATGAAGTAGTTCTTCGAATATAGAAAGT810.15404511049408542No Hit
CACAGGGGATTCCCCCGCAGCCAACGGGCCGCCCTTCCCCCGCGCCCCCTGC810.15404511049408542No Hit
TCTGCTGGGCTAACTATTGGCCTGAAGACTGGCACCAGCTCACCTATTTATT790.1502415275189228No Hit
TTTAGCGGGCTAACCAGGTCTTAAGAATAATGTAGAAGTCATGTTATATGGA790.1502415275189228No Hit
TTAGCGGTCCACACTGTGAAGGCAGTATTAGAAGACTTAATTGTAAAAGCTC780.1483397360313415No Hit
CGTTCAGGGATTCATGACCATTTTTCACTACCAAGCTCCCCCCCTCCCGAAG780.1483397360313415No Hit
GATTTAGGGAATTAAGTCACTCCTGAGAAAATATCCTTTTTTCTAGAAGAAA780.1483397360313415No Hit
GCAAATGGGGCTCTCGCAGCCGCCGCAGTCGCTGCGCCCCGAGCCCCTCTCC770.14643794454376022No Hit
AGTTGGGGGCGTGATGTCGTCACTTTTATGGCGTTAATTTCCTGCCACTACA770.14643794454376022No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTCGTATGCCTGCC760.14453615305617892No Hit
AATCTAGGGAAGAACTGGGATTAATGAAGCATTTTGTTTCATTTACACAAAT760.14453615305617892No Hit
TACATGCGGGATAGGTATTGTGAAATATTTTGCTAATCTTATAGAAAAGGAA760.14453615305617892No Hit
TTAATAGGGAAATTCTGATAGTTTCAAAATCTTTTATCTCCAGGTTTGATCT760.14453615305617892No Hit
ATACTTGGGAAATATGGGAGGGGGACTCAAATTTCAGAAAAGCTACCAAGTC750.14263436156859763No Hit
GATGTAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC750.14263436156859763No Hit
AACATGGGGCAGGGGGAGAATGAAAGGGGCGAGAGCTAAAGTGTGTTGTCAA740.14073257008101633No Hit
AGTCTAGGGATTTAAAAAAATGAAGACACTGAAGTACAGACTTAAATAGCTC730.138830778593435No Hit
GCTTTTGGGATGGGGAGAATTTTAAAATGTTAAACTGTAATTTGTATTTAAA730.138830778593435No Hit
TGAATCGGGCTGATGCCAGGCACTACAATCTCATAGGGTTGCACCCTTGATA720.13692898710585372No Hit
TTAGCCGGGGACACATGCACACGTATGTTTATTGCGGCACTATTCACAATAG720.13692898710585372No Hit
GGATATGGGTGATGTCTTCAGTGTGGGACTGGGGTCCTCTTGGTTCTGCCTG720.13692898710585372No Hit
CATTGGGGGTCTGGCCTGGATACAATAAATTTCAGAATCAATATCAATAGAG720.13692898710585372No Hit
GCAGTGGGGCTCGGGCATGTCTTTATTAGCAGTGTGAGAATGGACTAATACA720.13692898710585372No Hit
AACTACGGGGCCACTAAACACTGACTCGAATTCATATGATGCTGCTCACAGT720.13692898710585372No Hit
GACACTGGGAGAATCCTTCATGTCATTTTCTCTTTTCTTATTCATACCAGAG720.13692898710585372No Hit
GAATTAGGGAGAGTCACATTTTATTCTGATCAGAATTTTTATTGAGATGTTG710.1350271956182724No Hit
GAGATAGGGATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTACGA710.1350271956182724No Hit
TAGAGTGGGTAACTTGTGTGTCATGTCCAGTAATCAAGAAAAAAATTACAAG710.1350271956182724No Hit
GTAGTAGGGCTGCAGCTTGCTTCTCTGCAACAGAAATGACACTGCCTGGGAT700.1331254041306911No Hit
GCTCTTGGGGTCATGACCAGAGAATAATGCATTTAAATCCTATAGTAGTCCA700.1331254041306911No Hit
CTTCATGGGTGCCCTGAATTAAATGGCTGTTTTAACAGTAGGCTCTTAGCAT700.1331254041306911No Hit
TAGTCCGGGGATCTGCTGCTGGGGACTAGATCCAAACTGTGTTGTTCTTAAA690.1312236126431098No Hit
GCTCTTGGGATCTCCAGTCACAAAGAGAGACAGGAAATTTGGACTTTTAATT690.1312236126431098No Hit
TGGTTTGGGAATGAGGAATGTACTGGAAAAATAATCTGAAGAGTTGACAAAT690.1312236126431098No Hit
ATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTCG690.1312236126431098No Hit
ACAGTAGGGAGGTGTTGAAGGATCTTTGATAGTTGAGAAAATTATGCAAAGT690.1312236126431098No Hit
TTTCGGGCATTAATATAATAGCTTTGCTTGCAGCCTTCCAATCTATGTTGGT690.1312236126431098No Hit
AACTTGGGGGGCACTCGCGCAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGT680.1293218211555285No Hit
ATAGCTGGGATGTTGATTGATTGGGCAACTGACACTTCCAGAATCTAGGTGA680.1293218211555285No Hit
TTCCGTGGGGACTGGCGCAGGCGCAAGCCGGCAAGATGGCGGCGGCTGGGGC680.1293218211555285No Hit
CATGCAGGGGTTCATGTACTCTGTAAGCTAAGTCATCAGCTTCCTAGTCCAG670.12742002966794722No Hit
AATGATAGGGATGTGATTCCAGCTCATCAGTTTCCAGATGAATTAGCTGCAT670.12742002966794722No Hit
AGGCACGGGGAGCCTGCATTCTGCACACAGCATCTGCTCCCATCCCCCAAGT670.12742002966794722No Hit
TCTCTACGGGAAGGTAGCAGGCAGTCCAGCCCTGATGTGGAGACACATGGGA670.12742002966794722No Hit
AATAACAGGGAAACTAGAAGCAATGCTTTCTTTTCTTGGTATAATTTGTCTT660.12551823818036592No Hit
TATCGGGGATAGGCTATGTCCTCCCGTGAGGCCAAATATCATTCTGAGGGGC660.12551823818036592No Hit
TCGCGAGGGGTGGAAGCAATGGCTTCAACCCATTCCGTCTGCATCCACAAAA650.12361644669278461No Hit
GTCGCAGGGGTATTTGAATTTAGAGAAACAACATGGTTCCAAGAAGGAGGGT650.12361644669278461No Hit
AGACTTGGGATTGTACCTGTGAGAGTGAATTAAATTTCTAGAGAGTCATAAA650.12361644669278461No Hit
ATTGCGGGGGTTGAAAGTCTAAATAAAAGGCAGTTTAGGTGCCCTGGGTGCC640.1217146552052033No Hit
AAGCTTGGGGATTTTTAAGTCCCTTCCACTCTACTATATCACAATAATTTCC640.1217146552052033No Hit
CAAGTTGGGGATGCCCCTCATTTACATAAATATTATACTAGCATTTACCATC640.1217146552052033No Hit
GTTCCAGGGAAGGAAATGAAGGCAGAAATAAAGATGTTCTTTGAAACCAATG630.119812863717622No Hit
CTAGGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC630.119812863717622No Hit
TTTCATGGGATTGAAATTCTATCAATGCCTGTATCCCTGTAACCCCGAACTG630.119812863717622No Hit
AAATTAGGGTTGATCTCATATTGCGTGATATATTAACAGATTTTGTAATATT630.119812863717622No Hit
GACTCTGGGAGAATTCTTTGGGTTCTTTGTAATTTAATGAATAGGTCTGAAA630.119812863717622No Hit
CTTTATGGGCTGAGAGAAGCAGGCTCTCTATCCCTGTATTCCTATTCTCACG630.119812863717622No Hit
TCGCTTGGGCACATGCACTCCTCCACCGCTGAGAGTTGAATAGCTTTTCTTC630.119812863717622No Hit
CTACGTGGGGATCTCTTTACGGGATTGGAGCACATCAGTTTCTTGGGTAATG630.119812863717622No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTCGTATG630.119812863717622No Hit
CCACTAGGGGTATAGGGAGAATTTCATCTATCTAATCCACAAGAATCTTGAA620.11791107223004069No Hit
ATTAATGGGATGAAATGTAATGATGAAATTGAAATGAGATGAGTTGAAATGA620.11791107223004069No Hit
TCATGTGGGAAGATCCTGTGATGGCGAGACAAATGATCCTTAAAGAAGGTGT610.11600928074245939No Hit
TAGCCTGGGATAGCTTAGAGCTTTGTTCAATTCATTCGATAGTGTGATTCTT610.11600928074245939No Hit
AGCAATCGGGGTATCTGTAAGAGTAGGATCTGTTAGGATTAGTCATAAGACT610.11600928074245939No Hit
TAAAGTGGGATAAAAATGTAGTTATAACTATTTTTAATTCCAAGGTGTTGTT600.1141074892548781No Hit
TCAGGGGGACGCTCTTCCGATCTAGCAATGGGCTATATTTAATTCTCACAAT600.1141074892548781No Hit
ATAATAGGGAAATGTCTACTTCCAACATCCTTGAATTTGCAAGTGAAGGACA590.11220569776729679No Hit
TTAATAGGGGGGCTGGTTTTAGTACTCTGAAGGACAAAAACAAGCAAACAAA590.11220569776729679No Hit
AATGATGGGCTTTCAGCAAAGAGTTAATGGTATCCATTATGACTTTAATATC590.11220569776729679No Hit
TGCTTGGGGATGGAAAAAAGACTTTTTGCCACTGAAACTTGAGCCATCTGTG570.1084021147921342No Hit
GCTTGGGATGAAAAAATTAGATATTGTTTTGTGACTTTTAGACAGTGGTAAA570.1084021147921342No Hit
TCTGCTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC570.1084021147921342No Hit
GGTGGTGGGATTCCACCGACGTGGAGGCAGCCGTCAACAGCCTGGTCAATTT570.1084021147921342No Hit
AGTTGGGAGAATAAGCCTTTTAAGGTAAACCTCAAAATTATCATTTTATGGT570.1084021147921342No Hit
AATGTAGGGATTACCTCAGAAGTTTTTTTCTTCGCAGGATTTTTCTGAGCCT560.10650032330455289No Hit
TCACATGGGGCCTTGAATGGGGGTGAAAGCCTTTCTTTGTTGGATCAAATAC560.10650032330455289No Hit
TGTCTTGGACCTCAGTGCTGCAAGTATCACCAGAGAGGCTATGGAAGAATTT560.10650032330455289No Hit
GGTTTTGGGATCTTTGCTGGGATGCTTTCATCATTAAATTATCTGATAAAGT560.10650032330455289No Hit
TATTGTGGGATTGACGGAGCCTCCCTCAGGTGGAGGAACTACATATATGTAT550.1045985318169716No Hit
ATTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAATC550.1045985318169716No Hit
GGTTCAGGGGGAAGTGGACTGCGTTTATCAATACAGTCACAATGTTAAATGA550.1045985318169716No Hit
TAAGCCGGGGTATTACAATTAATTACTCTGACTGTAATAAAAGATATGTTAA550.1045985318169716No Hit
GCATCTGGGTGGGCTGGATGAAGAGCAAAGTGAAGCCAGCACAGATGGGGAG550.1045985318169716No Hit
TCGCTAGGGATTCTTTAGTGGGAATTAAGACCTACAAAGTCTAGTTTGTATG540.10269674032939027No Hit
TGTGTAGGGGTTTCTACTTTCCCTGATTTTCAAACTGGTTGCCTGCATCTTT540.10269674032939027No Hit
GCATACGGGTCTCTAGGGGATGGTGCATAAATACCTCAACTGTCTGGGCTGA540.10269674032939027No Hit
ATGTATGGGTATAACCAAATTGATCTTATTTTTGATTTCACTTATCATAGAA540.10269674032939027No Hit
AAAAGGGGATTATAAGACTAGTGGAAGGGATACGTTTTTAATGATGGATGAG540.10269674032939027No Hit
TGTTCCGGGAGAAATGCCAATTTCACTTTGAAACCATAGTCCAAGTCTCTAG540.10269674032939027No Hit
GTGCAAGGGAGAGGAAAGTGGGGTAAAATGCTACCTGTACGTCTGACATGAA530.10079494884180898No Hit
AATCTTGGGATGGAGAATGCTTAACAAATGCTCTTCTCCTTTTTCTGTCTTC530.10079494884180898No Hit
GTAGTAGGGAGGCATTGGATGCAGAACCTAGAGCCAGTTTTCAGGAACAATT530.10079494884180898No Hit
GCTGACCACCCTAGTGCCGCTGTTCCGGAACTCGCGCCTGGTCCAGTTCCAC530.10079494884180898No Hit
ACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTCGTAT530.10079494884180898No Hit
CAGCATGGGGGTACAAAATTTATGATAGAAAACAAAAGAAACTGACCGGATA530.10079494884180898No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)