FastQCFastQC Report
Sat 31 Dec 2022
SRR5846661.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846661.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51815
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTCGTATGC32296.231786162308212No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTCGTATGCCGTCT20894.031651066293544No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTCGTATGCCTGTC8961.7292289877448617No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTA6591.2718324809418122No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAA5721.1039274341406928No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAAT2630.5075750265367172No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAAACTCGTATGC1810.34931969506899546No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTCGT1740.33581009360223874No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGG1310.25282254173501884No Hit
ATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTC1230.23738299720158254No Hit
TTGAACGGGCTCTGCATCTGTTCTCTGAGGAACTCAAGTTTGGTTGCCAGAA1160.22387339573482581No Hit
GATCACGGGCTGGGGACTTGTTTTGGTTTGGGGGCAGCCATCCTCCACCAGA1140.2200135096014668No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTCGTATGCC1110.21422368040142814No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCCTGTCTCTTATAC1080.20843385120138955No Hit
ATACTTGGGAAATATGGGAGGGGGACTCAAATTTCAGAAAAGCTACCAAGTC1010.19492424973463282No Hit
GTTGCTGGGATGAAGTCATGAAGACCACCAAGATCATCATTGGCTGCTTTGT960.18527453440123517No Hit
GGCTTGGGGCAATATCATGTGTAGATATTATGTATAGAAAATAAAATAAATT950.18334459133455563No Hit
GGACCGGGGGAGAAGCAACTTCTAATACAAAGATGACTTATAACACATGAAA950.18334459133455563No Hit
TTTGACAGGGCAATTGCAAGCAAATAGATTTTTCTTTGTAACAATTTGTTCT940.1814146482678761No Hit
ATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTCG920.17755476213451704No Hit
TGGACTGACATCAGTTTGGGTGAGAGAGGGAGTCACTTTACTGAAAAGGCAG920.17755476213451704No Hit
TACATGCGGGATAGGTATTGTGAAATATTTTGCTAATCTTATAGAAAAGGAA910.1756248190678375No Hit
GACACTGGGAGAATCCTTCATGTCATTTTCTCTTTTCTTATTCATACCAGAG890.17176493293447845No Hit
TGTGGATGGGAAGGCATGATTTTTGGATTGTTTTCTTTCCACTTGTTGGCGG880.1698349898677989No Hit
CCTTCTGGGGTTCATTTGAAGCCATTTCTGTAAACATCTTCCAAGATAGAAC870.16790504680111937No Hit
CACAGGGGATTCCCCCGCAGCCAACGGGCCGCCCTTCCCCCGCGCCCCCTGC870.16790504680111937No Hit
CTCGCGGGGCTGTGGGAAGAACATAGCTGGAGCACAAGCATCAACAGCCAGC860.16597510373443983No Hit
GCAGTGGGGCTCGGGCATGTCTTTATTAGCAGTGTGAGAATGGACTAATACA860.16597510373443983No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCCTGTCTCT850.1640451606677603No Hit
CATTGGGGGTCTGGCCTGGATACAATAAATTTCAGAATCAATATCAATAGAG840.16211521760108077No Hit
GTTCCAGGGAAGGAAATGAAGGCAGAAATAAAGATGTTCTTTGAAACCAATG830.16018527453440123No Hit
TTTAGCGGGCTAACCAGGTCTTAAGAATAATGTAGAAGTCATGTTATATGGA830.16018527453440123No Hit
TATCGGGGATAGGCTATGTCCTCCCGTGAGGCCAAATATCATTCTGAGGGGC830.16018527453440123No Hit
TGCCGTGGGGGCAAGTTGCTTTGGCAGATATCATTCTCATTAATAAAACAGA820.1582553314677217No Hit
TTTCGGGCATTAATATAATAGCTTTGCTTGCAGCCTTCCAATCTATGTTGGT820.1582553314677217No Hit
AACTTGGGGGGCACTCGCGCAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGT810.15632538840104215No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACC810.15632538840104215No Hit
ATTAATGGGATGAAATGTAATGATGAAATTGAAATGAGATGAGTTGAAATGA810.15632538840104215No Hit
TGTCTAGGGGACCGACCCTCGACTTCGGAGAGGCAGCGCGGTTCCTCTGGGT800.15439544533436264No Hit
AGGCACGGGGAGCCTGCATTCTGCACACAGCATCTGCTCCCATCCCCCAAGT790.1524655022676831No Hit
TAAGCCGGGGTATTACAATTAATTACTCTGACTGTAATAAAAGATATGTTAA790.1524655022676831No Hit
CGTTCAGGGATTCATGACCATTTTTCACTACCAAGCTCCCCCCCTCCCGAAG780.15053555920100356No Hit
AATAACAGGGAAACTAGAAGCAATGCTTTCTTTTCTTGGTATAATTTGTCTT770.14860561613432405No Hit
GGTATGAGGGGTCATAACAGTCTTATGAAGTAGTTCTTCGAATATAGAAAGT760.1466756730676445No Hit
TGAATCGGGCTGATGCCAGGCACTACAATCTCATAGGGTTGCACCCTTGATA750.14474573000096497No Hit
TAATTGGGGATCTGCATAGGGGTTCCCCGCCCTGTATCCAAGTGTTCTCATT740.14281578693428545No Hit
GGTTCAGGGGGAAGTGGACTGCGTTTATCAATACAGTCACAATGTTAAATGA740.14281578693428545No Hit
GATTTAGGGAATTAAGTCACTCCTGAGAAAATATCCTTTTTTCTAGAAGAAA730.14088584386760591No Hit
TCTTCAGGGCACTGAAACTGCCTTATTCCTAGACTGAACTATTATACATGAA720.13895590080092637No Hit
AGTTGGGGGCGTGATGTCGTCACTTTTATGGCGTTAATTTCCTGCCACTACA720.13895590080092637No Hit
AAAAATGGGACCACCTTGAAGGGCCTTTTGCTTTCTGTGTTACCGTTTGCTG720.13895590080092637No Hit
GATGTAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC720.13895590080092637No Hit
TTAGCGGTCCACACTGTGAAGGCAGTATTAGAAGACTTAATTGTAAAAGCTC710.13702595773424683No Hit
GTGCAAGGGAGAGGAAAGTGGGGTAAAATGCTACCTGTACGTCTGACATGAA710.13702595773424683No Hit
CTAGGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC710.13702595773424683No Hit
AATCTAGGGAAGAACTGGGATTAATGAAGCATTTTGTTTCATTTACACAAAT700.13509601466756732No Hit
TAGCCTGGGATAGCTTAGAGCTTTGTTCAATTCATTCGATAGTGTGATTCTT700.13509601466756732No Hit
GTAAGCGGGATATTTAATGAAATTGGTGGTTTTAGGAAGTCAACTTTAGTTT690.13316607160088778No Hit
TGCTTGGGGATGGAAAAAAGACTTTTTGCCACTGAAACTTGAGCCATCTGTG690.13316607160088778No Hit
GTCGCAGGGGTATTTGAATTTAGAGAAACAACATGGTTCCAAGAAGGAGGGT690.13316607160088778No Hit
ATAATAGGGAAATGTCTACTTCCAACATCCTTGAATTTGCAAGTGAAGGACA690.13316607160088778No Hit
AATGATAGGGATGTGATTCCAGCTCATCAGTTTCCAGATGAATTAGCTGCAT690.13316607160088778No Hit
AACTACGGGGCCACTAAACACTGACTCGAATTCATATGATGCTGCTCACAGT690.13316607160088778No Hit
TAGTCCGGGGATCTGCTGCTGGGGACTAGATCCAAACTGTGTTGTTCTTAAA680.13123612853420824No Hit
GTCAAGGGGAGATGTTTTTCAGAGGCCTACAAATACAAGTATCTTTCTTATA680.13123612853420824No Hit
CCACTAGGGGTATAGGGAGAATTTCATCTATCTAATCCACAAGAATCTTGAA680.13123612853420824No Hit
TACATGGGGATTATTAGACAGTAATTTTGCTTTCCATCCAGTATGCTAGTTC680.13123612853420824No Hit
TATTTTGGGCTTGTTATCAAAACCCTTGTCCAGCACAACCTCTTTTATATGC670.1293061854675287No Hit
AGTCTAGGGATTTAAAAAAATGAAGACACTGAAGTACAGACTTAAATAGCTC670.1293061854675287No Hit
TCATCTGGGAATGAAATTCAATTTTAGTATTTCTAATAATATTATTTCTGTT670.1293061854675287No Hit
ACGATTGGGACAGATATGCAAGACAATGTCTTGTTTAATTTATTAAATTAGA660.1273762424008492No Hit
TCTGCTGGGCTAACTATTGGCCTGAAGACTGGCACCAGCTCACCTATTTATT660.1273762424008492No Hit
GTAGTAGGGCTGCAGCTTGCTTCTCTGCAACAGAAATGACACTGCCTGGGAT660.1273762424008492No Hit
ACTAATGGGCAACCAGATTTAGGGCTCTTCTTAATTGATCATGTTCAGAAAG660.1273762424008492No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTCGTATGCCTGCC660.1273762424008492No Hit
ACGTGCGGGACCGACTAATCACCACCCAACAATGACTAATCAAACTAACCTC660.1273762424008492No Hit
TCAGGGGGACGCTCTTCCGATCTAGCAATGGGCTATATTTAATTCTCACAAT650.12544629933416965No Hit
TCCCACATGGGAAGGTATGTGGACCGAGTGACTGAATTCCTCCAGCAAAAGC650.12544629933416965No Hit
GCTTTTGGGATGGGGAGAATTTTAAAATGTTAAACTGTAATTTGTATTTAAA650.12544629933416965No Hit
GGTTTTGGGATCTTTGCTGGGATGCTTTCATCATTAAATTATCTGATAAAGT650.12544629933416965No Hit
CATGCAGGGGTTCATGTACTCTGTAAGCTAAGTCATCAGCTTCCTAGTCCAG640.12351635626749011No Hit
GGTGTTAGGGATAGTGTTAGTGTGATGCTATACTATTGGAAAAATAGCAGCT640.12351635626749011No Hit
AACATGGGGCAGGGGGAGAATGAAAGGGGCGAGAGCTAAAGTGTGTTGTCAA640.12351635626749011No Hit
GAATTAGGGAGAGTCACATTTTATTCTGATCAGAATTTTTATTGAGATGTTG630.12158641320081057No Hit
ATTGCGGGGGTTGAAAGTCTAAATAAAAGGCAGTTTAGGTGCCCTGGGTGCC630.12158641320081057No Hit
TTAATAGGGGGGCTGGTTTTAGTACTCTGAAGGACAAAAACAAGCAAACAAA630.12158641320081057No Hit
TGCAGCGGGAGCGCAAGTTGTCTTTGCCATCTGGTCTATAGTACAGTGCGCG630.12158641320081057No Hit
ATAGCTGGGATGTTGATTGATTGGGCAACTGACACTTCCAGAATCTAGGTGA630.12158641320081057No Hit
TCTGCTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC630.12158641320081057No Hit
TCTAGAGGGACTTACAAGAGATGTGAAGGGCCTCTTCAAAGAGAACTACACA630.12158641320081057No Hit
GCAAATGGGGCTCTCGCAGCCGCCGCAGTCGCTGCGCCCCGAGCCCCTCTCC620.11965647013413103No Hit
TGGTTTGGGAATGAGGAATGTACTGGAAAAATAATCTGAAGAGTTGACAAAT620.11965647013413103No Hit
ACATCTGGGAGAACTGATAATGTAGCAAGCCCCAAGTATGATAATAGGCAGA620.11965647013413103No Hit
TCATGTGGGAAGATCCTGTGATGGCGAGACAAATGATCCTTAAAGAAGGTGT620.11965647013413103No Hit
CTTCATGGGTGCCCTGAATTAAATGGCTGTTTTAACAGTAGGCTCTTAGCAT620.11965647013413103No Hit
AATGATGGGCTTTCAGCAAAGAGTTAATGGTATCCATTATGACTTTAATATC620.11965647013413103No Hit
TGTTCCGGGAGAAATGCCAATTTCACTTTGAAACCATAGTCCAAGTCTCTAG610.11772652706745151No Hit
AGCAAGGTGGATGGAACTGGATGTCATTTTGTTGAGTGAATTAAGCCCAGGC600.11579658400077197No Hit
GGCTTTGGGATAAAACCAAATGCTTGTCTGCTAGCATACTTTTCAAAGGGAA600.11579658400077197No Hit
TAGAGTGGGTAACTTGTGTGTCATGTCCAGTAATCAAGAAAAAAATTACAAG600.11579658400077197No Hit
CAAGTTGGGGATGCCCCTCATTTACATAAATATTATACTAGCATTTACCATC600.11579658400077197No Hit
GTATTAGGGACCAATTAATCAGTTAATTTCTGAAGACTTTGTATAGGACTTG600.11579658400077197No Hit
ATTGGAGGGTAATATATATACTTAATATTACCTCCAATGACTCGGGAATTGC590.11386664093409245No Hit
GCTTATCGGGGCGGATGGGAGAGTTTCTTTACATTGCCGTGGCATCTTAAAA590.11386664093409245No Hit
AAATTAGGGTTGATCTCATATTGCGTGATATATTAACAGATTTTGTAATATT590.11386664093409245No Hit
AAGCTTGGGGATTTTTAAGTCCCTTCCACTCTACTATATCACAATAATTTCC590.11386664093409245No Hit
TACAACGGGAACTAGAAGGATAACTAGTAATGCATCAACATAATTTCTGTAT590.11386664093409245No Hit
TCGCGAGGGGTGGAAGCAATGGCTTCAACCCATTCCGTCTGCATCCACAAAA580.11193669786741291No Hit
CTACGTGGGGATCTCTTTACGGGATTGGAGCACATCAGTTTCTTGGGTAATG580.11193669786741291No Hit
TCTATTGGGAATGAAAGGAAGTACCAATTAGTTGATTTGTTGGTGGCATTCC580.11193669786741291No Hit
TATCAGGGGAGTGATATTGTAACAATACTGCCATTCCCTTCTACTGCACTGC580.11193669786741291No Hit
ATAAAGGGATGGCTTGGAGTTACCTTATATTCAGGTATATGTGATATTTTGC570.1100067548007334No Hit
CACGGTGGGCTTAGGAGAAAACTTAAAAATATATGAATACATGCGCAATACA570.1100067548007334No Hit
ATTTTAGGGCCGATCTGGGTGTGGGGGTGGAGAGAGCGCGGAGACAAGCGCA570.1100067548007334No Hit
TTCCGTGGGGACTGGCGCAGGCGCAAGCCGGCAAGATGGCGGCGGCTGGGGC570.1100067548007334No Hit
TTAGCCGGGGACACATGCACACGTATGTTTATTGCGGCACTATTCACAATAG560.10807681173405385No Hit
GGATATGGGTGATGTCTTCAGTGTGGGACTGGGGTCCTCTTGGTTCTGCCTG560.10807681173405385No Hit
ATAAAATGAGAATTATGGCATAATTGGAGCATTTGCATTAATCAACAAACTC560.10807681173405385No Hit
GAGATAGGGATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTACGA560.10807681173405385No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTC560.10807681173405385No Hit
TTAATAGGGAAATTCTGATAGTTTCAAAATCTTTTATCTCCAGGTTTGATCT560.10807681173405385No Hit
TCGCTAGGGATTCTTTAGTGGGAATTAAGACCTACAAAGTCTAGTTTGTATG550.10614686866737431No Hit
CAAAAAGGGATGTTCTTAAAGTTCCTTTTTTTAATTTACTTTTTGGAAAAAC550.10614686866737431No Hit
AGCCTGGGGCGGCAACAGCACGGCCATCCAGGAGCTGTTCAAGCGCATCTCC550.10614686866737431No Hit
GACTCTGGGAGAATTCTTTGGGTTCTTTGTAATTTAATGAATAGGTCTGAAA550.10614686866737431No Hit
GTATACGGGCTGTGGTTCCCGGTGGGGGTGCTGTAGAAGCAGCCCTTTCCAT550.10614686866737431No Hit
AAAAGGGGATTATAAGACTAGTGGAAGGGATACGTTTTTAATGATGGATGAG550.10614686866737431No Hit
TTTCATGGGATTGAAATTCTATCAATGCCTGTATCCCTGTAACCCCGAACTG540.10421692560069477No Hit
GTTCACGGGAGGACCTGGCTCGTGAGTGCGTCTGGGTCAGGAAGAGACCTCT540.10421692560069477No Hit
TTCTTTGGGAATGATGATTATACCTTTTCAAAATTAAGTGACTTCCTAAGAA530.10228698253401523No Hit
TTTGCTGGGATCCTGGGAAATCCAGCTACCATGGCCCTCCCAGTGGAGGCAT530.10228698253401523No Hit
GTTCTAGGGAGTTTAATGTTTCCAGAAAGCCAAAGTAATTTTCTTTTCAGAT530.10228698253401523No Hit
GCATACGGGTCTCTAGGGGATGGTGCATAAATACCTCAACTGTCTGGGCTGA530.10228698253401523No Hit
TAACAGGGGAAGTGGAAATCAGGATTGTTATGAAGACTTAAGGCCCAGTATT530.10228698253401523No Hit
AATCTTGGGATGGAGAATGCTTAACAAATGCTCTTCTCCTTTTTCTGTCTTC520.10035703946733572No Hit
GTACAGGGGTGCAGACTACAGTGGCCAACAGAGCACTTATGGCAAGGCATCT520.10035703946733572No Hit
GCTCTTGGGGTCATGACCAGAGAATAATGCATTTAAATCCTATAGTAGTCCA520.10035703946733572No Hit
GTTCGAGGGCGGACTCTCTGCTGCGGAGGGGGGCAATACCAGTGACACCCAG520.10035703946733572No Hit
TACCAGGGGACTTTTAAAACGGATGGCCTTCTTAAGAATAAAGCAAAAGGTG520.10035703946733572No Hit
TGTCTTGGACCTCAGTGCTGCAAGTATCACCAGAGAGGCTATGGAAGAATTT520.10035703946733572No Hit
GCATCTGGGTGGGCTGGATGAAGAGCAAAGTGAAGCCAGCACAGATGGGGAG520.10035703946733572No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCTTAATCTCGTATG520.10035703946733572No Hit
TTTGCGCGGGAACATGTTCCATTCTAAGTCTTGCCCTTGCATGTGGTTGAAA520.10035703946733572No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)