FastQCFastQC Report
Sat 31 Dec 2022
SRR5846644.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846644.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160852
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGACTATGATCTCGTATGCCGTCT8160.5072986347698505No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGACTATGATCTCGTATGC7380.45880685350508543No Hit
GGTGAGGGGATCAGGAAATCAGGAAGTATAAATCAGGAAAACATTGGTGGGT2260.14050182776714No Hit
TTTATTGGGTCCCCAGTGATTGTTTTCTTTGACTCAGTGGTTAAGAGTGAAT2060.12806803769925149No Hit
CAATGCGGGGCTTTCTTCTTCTTGGAAATAGTCCTCCAAAAAGTCCACTTGT2040.12682465869246262No Hit
CAACCAGGGTGAGTGTTGGAAAGCATATTGTTGGATAAATGGACAAAATTGG1950.1212294531619128No Hit
ACATGCGGGAGGAGGCATCGGGGACAGCCGCTGCGGCAGCACTCGAGCCAGC1950.1212294531619128No Hit
GGAAATGGGACTTGTCTCCTCGAACGTGATTTGTGGTATATTTCATGACACA1920.11936438465172954No Hit
GAGGTCCGGGATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTA1900.11812100564494069No Hit
GTAATGGGGATGGTGGTACCAGGAGACGATAAAGTAGAAAGTTGGAGTCAAA1900.11812100564494069No Hit
ATTTGGGGGATCTTCTTCGAAACCACACTTATCCCCACCTTGGCTATCATCA1900.11812100564494069No Hit
AAACTCTGGGGGGCGCTGAGAACGCGGGTCCACGCGTGTGATCGTCCGTGCG1890.11749931614154627No Hit
ACATGGAGGGGAAGTTTGTTTTTTAAATGACCACAAGTGTAAGACTGAATGA1890.11749931614154627No Hit
ATTCACGGGGTTGGTAAACTGTTTATCCCCAAGCAGTAAATGAAATATTTGT1880.11687762663815185No Hit
AAGCTAGGGACTCCATGCAAATGGAAACCAAAAGAGAACAGGGTAACTATAC1870.1162559371347574No Hit
CGATAAGGGATCTTCAGAACAGCAACATTTGAAGGGCCAGACTGGCACCAAC1870.1162559371347574No Hit
GTATGCGGGAACCCTTTGATTTTCAAGAACTACCGAATAAGTGTATGAAGAG1860.11563424763136299No Hit
ACAAACGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1850.11501255812796857No Hit
TACTCCGGGCGATCTTGTCAGGGGATCTTTAAAAAAGATCTCACAGATTCAT1840.11439086862457414No Hit
TTAAAAGGGCACCTTTTTAAAAAAGCACTGGGACCTTTTCTTTAAACAAAAT1810.11252580011439087No Hit
TAAAGTGGGGTGATGGTGCATTTGATTGAAGCAGCTTGTCTTTATTATGCAA1800.11190411061099645No Hit
TTCTCAGGGAAATCAAGTTGAGTTGATTGCAAATACATAATTTTTAAAGTAG1800.11190411061099645No Hit
TTCTCTGGGAGAGAAAACAGCAGCTGCAGAATTAAAATCATACAAAACCAAA1790.11128242110760203No Hit
AAAATAGGGGACACTGAATCTTTTTTATTCTTACTCTAAAATTATTCTGGCT1780.11066073160420759No Hit
TGCGTAGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC1780.11066073160420759No Hit
GCTTTAGGGATTTTAATAAACCTTCTACCATGTAGAAGTGAAATAAATGATC1770.11003904210081317No Hit
TGGATAGGGGCCGCTCAGATATGAAGCATCAACAAACGAATTGATTAATGAA1770.11003904210081317No Hit
CATAAAGGGAGAATATTTTTTATTAGGAAAAATTGCAATAAGAAAAATCCAG1770.11003904210081317No Hit
TTGGGAGGGGAGGTACGTGGGCGGGCGGATCGTGGTGGCTGCGCTTGCGAGG1750.10879566309402432No Hit
CAGTAAGGGATTTCTTGTGTGACCTGATAGCTCCCTGGAACTTTGGGTCTGT1750.10879566309402432No Hit
GTATTCGGGGTGGATTATTTTATTGCTCTGGAGTCTGGAAGCTGACTATGAT1740.1081739735906299No Hit
TATTCAGGGCTGTCCTCTTCCTGTTAGTGTTCTACAATTCCTTTCTCCTTAA1730.10755228408723548No Hit
GGTTGCGGGTACGGCCTGTTCATGCGGGAGGGCATTGTGACCGCCACCGAGC1720.10693059458384103No Hit
CTTATAGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA1710.10630890508044663No Hit
TTGCAGGGGTCCTTAGGAAGTTATTCCAAACCTCTGGTGTGGGGCTAAGGGA1710.10630890508044663No Hit
GAGCGGGGGTGAAAAACAGTGTCTTTCAAGGTTGTACCTAAGAGTAAGGTGG1710.10630890508044663No Hit
TTAGTGGGGATGGACTGTGGAGGACTGTTGTGGGAAGTAGTTAGTTTATATT1700.10568721557705221No Hit
ATGAGTGGGGACAGAAGGAAGGAAGAAAGTGAAAACCACAAAACAACCAGAA1700.10568721557705221No Hit
AGCTTTGGGAGATAAAGTAATTTTTGTTTGAACTTCATCTAGAATTTGGAGT1700.10568721557705221No Hit
GCACATGGGATGACCACCGTCGGCTACGGCGACATCTACCCCAAGACCACGC1690.10506552607365777No Hit
GATCTGGGTAATTTCCTATTTTAAAAAAGTTTGCTTTTTAATTTGCTCTTTA1690.10506552607365777No Hit
GCAGTGGGGCAGCTTTTGTATTGCAATAACCTACAATCTCAATTCTCAAAGG1690.10506552607365777No Hit
CACGTAGGGGCTTCACACTGCAATGGTAGGGTTGAATACTTGCAATAAAGAC1690.10506552607365777No Hit
TTGAGAGGGAGATGAGAACAGTGAGCCCCAGAGGAGGGGAATGGCTGTCCAG1680.10444383657026335No Hit
TGACCTGGGCCGGTTGACTTTGCGGAGCCATGGAGGGCGGCTTCGGCTCCGA1670.10382214706686893No Hit
ACTCTAGGGTATGTGGACATATTCAACTATGTCACAACTGCAGCCTGGACAA1660.1032004575634745No Hit
CTGAGGGTGAGATAACTCTGGGGAATTAATTTTATGAGATAAGATGAATGGC1660.1032004575634745No Hit
TACTGTGGGGGCGCGTTTCGGGTGCTGGCGGCTGCAGCCGGAGTTCAAACCT1660.1032004575634745No Hit
ACGTTAGGGAGAGGCTTACAACTTGCCTATTCCAAGTCCAGAATCCCTGTAA1650.10257876806008008No Hit
AGACTGGGCGCTTGCAACCTCCGGGACCATCTTCTCGGCCATCTCCTGCTTC1650.10257876806008008No Hit
CGCTTTGGGGGATGGAGAAAACCATAGTCTTCCTTCTATTGTTAGAGATTTC1650.10257876806008008No Hit
GATGCTGGGCAGGTAGAATTAAAACAAACTGCTAGTATTACCAATTTTCACA1640.10195707855668563No Hit
TCAGCTGGGACTACAAACATGCACTGCAGCACTTGACTTAGTAAAAACTTTT1640.10195707855668563No Hit
TGAATCGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA1630.10133538905329122No Hit
TTACTTAGGGAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGAT1630.10133538905329122No Hit
TGGTCTGGGAACAACTGCTACCATGCATCCACAAGTATGTCTGATAGGGGAA1630.10133538905329122No Hit
GAAGGGGATCCAAGAGCAAAATTGTTTAATGGTTTCATTGACGTTTTCAGTT1630.10133538905329122No Hit
TTGTCGGGAGTGGAGACGGGGTTTCACCATGTTGGCCAGGATGGTCTCAATC1620.1007136995498968No Hit
TTGTTGGGAGAAATGGAAAGTGGGACTGAGAGGGAGTCAGCAGGCATGCTGC1620.1007136995498968No Hit
TTTACACCCCAGCAGCAACAATGGAAGACATAGTGGAATTAAATAGGCAGGG1610.10009201004650237No Hit
GTCGCGGGGACGCTCAGCCTTTAATTTTATTATTATAAAAAATTGAGCCAGG1610.10009201004650237No Hit
CAAGTTGGGTGGAAACATTCTAGAGGAAATTAAAAATACAGGACCTTTGGGA1610.10009201004650237No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)