FastQCFastQC Report
Sat 31 Dec 2022
SRR5846643.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846643.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161276
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGACTATGATCTCGTATGCCGTCT7660.47496217664128576No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGACTATGATCTCGTATGC7410.4594608001190506No Hit
GGTGAGGGGATCAGGAAATCAGGAAGTATAAATCAGGAAAACATTGGTGGGT2310.143232719065453No Hit
ATTTGGGGGATCTTCTTCGAAACCACACTTATCCCCACCTTGGCTATCATCA2110.13083161784766487No Hit
TAAAGTGGGGTGATGGTGCATTTGATTGAAGCAGCTTGTCTTTATTATGCAA2060.12773134254321783No Hit
ATTCACGGGGTTGGTAAACTGTTTATCCCCAAGCAGTAAATGAAATATTTGT1980.12277090205610258No Hit
CAAGTTGGGTGGAAACATTCTAGAGGAAATTAAAAATACAGGACCTTTGGGA1960.12153079193432377No Hit
CAATGCGGGGCTTTCTTCTTCTTGGAAATAGTCCTCCAAAAAGTCCACTTGT1940.12029068181254494No Hit
GAGGTCCGGGATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTA1930.11967062675165555No Hit
TTCTCAGGGAAATCAAGTTGAGTTGATTGCAAATACATAATTTTTAAAGTAG1900.11781046156898733No Hit
CGCTTTGGGGGATGGAGAAAACCATAGTCTTCCTTCTATTGTTAGAGATTTC1890.11719040650809791No Hit
TCAAAGGGACGCTGAGGAGTTTGGCAACGAGACGTGGGGCGTAACAAAGGCA1890.11719040650809791No Hit
TGAATCGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA1850.1147101862645403No Hit
TTTATTGGGTCCCCAGTGATTGTTTTCTTTGACTCAGTGGTTAAGAGTGAAT1840.11409013120365087No Hit
CTGTTGGGGAGAAGAGTTAGGCTAGGACTTGTCATAAGGGCACCTGACTCCA1840.11409013120365087No Hit
AAACTCTGGGGGGCGCTGAGAACGCGGGTCCACGCGTGTGATCGTCCGTGCG1820.11285002108187207No Hit
CGATATGGGAAAGGCAAGCTGGAAGGCACATTGTATCAATTCTACCTTGTGC1810.11222996602098267No Hit
GCTCACGGGGTGGAAAAATCATGAGTTAACAAATTATTCTGGCATAGGTAGT1810.11222996602098267No Hit
ACAGCAGGGTGCAAGAGTGAATTTTATGACAAATTTTCTGAAGAGTGTACAT1810.11222996602098267No Hit
GCATTTGGGGAGGTGCAGCAAATAGAGAAGGCATGTCAAACCCTGCATTTCT1800.11160991096009326No Hit
CAAAATGGGTCTACAAGGAGAAAACTTCTCAAAATAGTCATCTGTTACCTAG1800.11160991096009326No Hit
GCTTTAGGGATTTTAATAAACCTTCTACCATGTAGAAGTGAAATAAATGATC1790.11098985589920385No Hit
CGATAAGGGATCTTCAGAACAGCAACATTTGAAGGGCCAGACTGGCACCAAC1790.11098985589920385No Hit
CAACCAGGGTGAGTGTTGGAAAGCATATTGTTGGATAAATGGACAAAATTGG1780.11036980083831444No Hit
GTCGCGGGGACGCTCAGCCTTTAATTTTATTATTATAAAAAATTGAGCCAGG1780.11036980083831444No Hit
GGAAATGGGACTTGTCTCCTCGAACGTGATTTGTGGTATATTTCATGACACA1780.11036980083831444No Hit
TTACTTAGGGAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGAT1780.11036980083831444No Hit
GAAAATGGGACAATAGCAGAAGGGTGGACACAACTCAAGGGTCTATAAACAG1780.11036980083831444No Hit
ATGAGTGGGGACAGAAGGAAGGAAGAAAGTGAAAACCACAAAACAACCAGAA1760.10912969071653564No Hit
CTTCAAGGGCCCATACATCTCAAACAGGGACCAGAGCAAGGGAAATAAATAC1750.10850963565564621No Hit
TGTAGTGGGATGACAAAAGAAGTGTGACTTCCATTTTACTCTGATGCTATAT1750.10850963565564621No Hit
ACATGGAGGGGAAGTTTGTTTTTTAAATGACCACAAGTGTAAGACTGAATGA1730.1072695255338674No Hit
GTATTCGGGGTGGATTATTTTATTGCTCTGGAGTCTGGAAGCTGACTATGAT1720.106649470472978No Hit
TGGTCTGGGAACAACTGCTACCATGCATCCACAAGTATGTCTGATAGGGGAA1720.106649470472978No Hit
TTCTCTGGGAGAGAAAACAGCAGCTGCAGAATTAAAATCATACAAAACCAAA1720.106649470472978No Hit
GTTGGGGGGCAACACTGTAGCCAGAATATTTCTGATTGCATATTCATAGCAT1710.1060294154120886No Hit
TTAAAAGGGCACCTTTTTAAAAAAGCACTGGGACCTTTTCTTTAAACAAAAT1710.1060294154120886No Hit
TCAGCTGGGACTACAAACATGCACTGCAGCACTTGACTTAGTAAAAACTTTT1710.1060294154120886No Hit
AGCTTTGGGAGATAAAGTAATTTTTGTTTGAACTTCATCTAGAATTTGGAGT1710.1060294154120886No Hit
AGTTTCGGGATAATCACAGATTTTTAGACAAATTTCTTGTATCAGGAAGAAA1700.10540936035119917No Hit
TTGTAGGGGAAGCAAACATTTTAATGAGCAAAAGTTAGTGTTATTAAAGTTT1700.10540936035119917No Hit
AAGCTAGGGACTCCATGCAAATGGAAACCAAAAGAGAACAGGGTAACTATAC1700.10540936035119917No Hit
TACTGTGGGGGCGCGTTTCGGGTGCTGGCGGCTGCAGCCGGAGTTCAAACCT1700.10540936035119917No Hit
TGACCTGGGCCGGTTGACTTTGCGGAGCCATGGAGGGCGGCTTCGGCTCCGA1690.10478930529030978No Hit
TCAGCTGGGAAGGTTCAAAATCAAGTGCCTAGGGAAAATTCCAATGGATGAT1690.10478930529030978No Hit
GATCTGGGTAATTTCCTATTTTAAAAAAGTTTGCTTTTTAATTTGCTCTTTA1680.10416925022942038No Hit
TCCATGGGGGACTCGCGGCATTGGAGTCAAGATGGAGGAGTACGCGCGAGAG1670.10354919516853096No Hit
TCTGTGGGGCCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTT1670.10354919516853096No Hit
GCACATGGGATGACCACCGTCGGCTACGGCGACATCTACCCCAAGACCACGC1660.10292914010764156No Hit
CTCTGTGGGGAGATATAAATTAATAATATAATTTCTGGCTTAGTACCTGGTA1660.10292914010764156No Hit
CCACTAGGGGGGTTTCTTCCGAGACCTCTCTGTCTGGCTTGTAGATGGATAC1660.10292914010764156No Hit
AAGCCGGGGGCACAGTCTTGGCACCCGCCTTTCCCGTGTGCGATCTCTGGAG1650.10230908504675214No Hit
CAACATGGGACCAGATGTACAGCTTCCACATTCTCTCATTATCTCACATTTC1650.10230908504675214No Hit
CAGTAAGGGATTTCTTGTGTGACCTGATAGCTCCCTGGAACTTTGGGTCTGT1650.10230908504675214No Hit
TTATAAGGGATGCTGGGCAATGGCTATTAACAATTAATTGTCTTGCACTGGC1640.10168902998586274No Hit
CTGCACGGGTATGGCGGCTGGTACAAAGCCTGCAAAGTTGATAGTCCAACTG1640.10168902998586274No Hit
CACGTAGGGGCTTCACACTGCAATGGTAGGGTTGAATACTTGCAATAAAGAC1640.10168902998586274No Hit
ACATGCGGGAGGAGGCATCGGGGACAGCCGCTGCGGCAGCACTCGAGCCAGC1640.10168902998586274No Hit
AGGATGGGGGTGAGCACCATGTGCAAGATTTCCAAGTCTTTCAAAGCTTGTG1640.10168902998586274No Hit
AGAACTGGGACCAAGCGGAAGCATGAGACCCGCCCACACTAGCCTCTGTGTT1630.10106897492497334No Hit
TGCGTAGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC1630.10106897492497334No Hit
ACAAACGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1630.10106897492497334No Hit
AATTGAGGGCAACCTCTAGGATTTTGCATGCAGAGCGGATCCTGCCTTGTCA1620.10044891986408394No Hit
CGTAGGGGGGGCAACAGAGTAAGACTCTGTCTCAAAAATGAATGAATGAATG1620.10044891986408394No Hit
TTGAGAGGGAGATGAGAACAGTGAGCCCCAGAGGAGGGGAATGGCTGTCCAG1620.10044891986408394No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)