FastQCFastQC Report
Sat 31 Dec 2022
SRR5846642.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846642.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159215
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGACTATGATCTCGTATGCCGTCT7400.4647803284866376No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGACTATGATCTCGTATGC7180.45096253493703486No Hit
GGTGAGGGGATCAGGAAATCAGGAAGTATAAATCAGGAAAACATTGGTGGGT2370.14885532142072042No Hit
CAAGTTGGGTGGAAACATTCTAGAGGAAATTAAAAATACAGGACCTTTGGGA2210.1388060170210093No Hit
TAAAGTGGGGTGATGGTGCATTTGATTGAAGCAGCTTGTCTTTATTATGCAA2200.1381779354960274No Hit
CAATGCGGGGCTTTCTTCTTCTTGGAAATAGTCCTCCAAAAAGTCCACTTGT2090.131269038721226No Hit
CAACCAGGGTGAGTGTTGGAAAGCATATTGTTGGATAAATGGACAAAATTGG1960.12310397889646076No Hit
TTCTCTGGGAGAGAAAACAGCAGCTGCAGAATTAAAATCATACAAAACCAAA1960.12310397889646076No Hit
GCTCACGGGGTGGAAAAATCATGAGTTAACAAATTATTCTGGCATAGGTAGT1900.1193354897465691No Hit
TGGTCTGGGAACAACTGCTACCATGCATCCACAAGTATGTCTGATAGGGGAA1870.11745124517162327No Hit
TACTGTGGGGGCGCGTTTCGGGTGCTGGCGGCTGCAGCCGGAGTTCAAACCT1870.11745124517162327No Hit
TTCTCAGGGAAATCAAGTTGAGTTGATTGCAAATACATAATTTTTAAAGTAG1860.11682316364664133No Hit
CTTAGGACGGGAGAAATAAAATACTGAGGAATATGTTTAACCAATGAGGCGA1820.11431083754671356No Hit
ATTCACGGGGTTGGTAAACTGTTTATCCCCAAGCAGTAAATGAAATATTTGT1810.11368275602173163No Hit
AGGATGGGGGTGAGCACCATGTGCAAGATTTCCAAGTCTTTCAAAGCTTGTG1790.11242659297176774No Hit
AAGCTAGGGACTCCATGCAAATGGAAACCAAAAGAGAACAGGGTAACTATAC1780.11179851144678579No Hit
TGCAAAGGGAGGCTGAGGTGGGAGGATTGCTTGAGCCCAGGAGGCAGAGGTT1780.11179851144678579No Hit
CGCTTTGGGGGATGGAGAAAACCATAGTCTTCCTTCTATTGTTAGAGATTTC1770.11117042992180384No Hit
TTGGGAGGGGAGGTACGTGGGCGGGCGGATCGTGGTGGCTGCGCTTGCGAGG1750.10991426687183997No Hit
GCACATGGGATGACCACCGTCGGCTACGGCGACATCTACCCCAAGACCACGC1740.10928618534685802No Hit
TCAGCTGGGACTACAAACATGCACTGCAGCACTTGACTTAGTAAAAACTTTT1740.10928618534685802No Hit
TGCAAAGGGGAGAATACAGGAAATGGCTCTTGTTTATTTTTTTGCTGTGTTT1740.10928618534685802No Hit
GAAGGGGATCCAAGAGCAAAATTGTTTAATGGTTTCATTGACGTTTTCAGTT1730.10865810382187607No Hit
GGCATTGGGAGGTGTACTTTGCTTCTCACAATGATTGTGCAAAAAGTCACGT1720.10803002229689415No Hit
ATGAGTGGGGACAGAAGGAAGGAAGAAAGTGAAAACCACAAAACAACCAGAA1720.10803002229689415No Hit
GGATATGGGAGGTCTGCCTTTTTTTCTGGAGAATAAATGCAGTAATCCTCTC1720.10803002229689415No Hit
TGCTGTGGGGGATGAGAGGCGAAAGCCACTTCTCATACAAGCCCCTTTATTG1710.1074019407719122No Hit
GAGGCAGGAAAATCGCTTGAACCTGGGAGGCAGAGGTTGCAGTGAGCCGAGA1700.10677385924693025No Hit
GTATTCGGGGTGGATTATTTTATTGCTCTGGAGTCTGGAAGCTGACTATGAT1690.1061457777219483No Hit
TTGTAGGGGAAGCAAACATTTTAATGAGCAAAAGTTAGTGTTATTAAAGTTT1690.1061457777219483No Hit
GGAAATGGGACTTGTCTCCTCGAACGTGATTTGTGGTATATTTCATGACACA1690.1061457777219483No Hit
ATTTGGGGGATCTTCTTCGAAACCACACTTATCCCCACCTTGGCTATCATCA1690.1061457777219483No Hit
ACATGGAGGGGAAGTTTGTTTTTTAAATGACCACAAGTGTAAGACTGAATGA1670.10488961467198443No Hit
ACATGCGGGAGGAGGCATCGGGGACAGCCGCTGCGGCAGCACTCGAGCCAGC1670.10488961467198443No Hit
CAACTCGGGCTGTTGTGGCTTTAATGGACAACAGGAGTTGGGAAACTTTCTG1660.10426153314700248No Hit
TAAAGGGGGGGCGAAGAGGAGATGGCGGAGTCGTTGAGGTCTCCGCGCCGCT1660.10426153314700248No Hit
CAACATGGGACCAGATGTACAGCTTCCACATTCTCTCATTATCTCACATTTC1660.10426153314700248No Hit
CTGAGGGTGAGATAACTCTGGGGAATTAATTTTATGAGATAAGATGAATGGC1650.10363345162202053No Hit
TGAAAAGGGTACTGCAGAGCAGCGTTAATGGAGTGCTTACTTTACATGAGCT1640.10300537009703858No Hit
ACAGCAGGGTGCAAGAGTGAATTTTATGACAAATTTTCTGAAGAGTGTACAT1640.10300537009703858No Hit
TCTAAGGGGAGTTCAACTGTTGATCAATTATCTTTGAGACTTTTGACATTCA1640.10300537009703858No Hit
AAAATAGGGGACACTGAATCTTTTTTATTCTTACTCTAAAATTATTCTGGCT1630.10237728857205666No Hit
TTACTTAGGGAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGAT1630.10237728857205666No Hit
CGAAAAGGAAGCCAACATCAGAGCAAGGAAAATTATCAGAGATAAAGAGGAA1630.10237728857205666No Hit
GTTGGGGGGCAACACTGTAGCCAGAATATTTCTGATTGCATATTCATAGCAT1620.10174920704707471No Hit
TTTATTGGGTCCCCAGTGATTGTTTTCTTTGACTCAGTGGTTAAGAGTGAAT1620.10174920704707471No Hit
TTACTAGGGTACTCAGCCATGCCCTTTCATTTTGACGATAGTGTTTCTAATA1620.10174920704707471No Hit
TTGAGAGGGAGATGAGAACAGTGAGCCCCAGAGGAGGGGAATGGCTGTCCAG1620.10174920704707471No Hit
TACGAAGGGCTAGTCGAGGGGCACAAGAAAGATTGAAAAGTACCAGAAAGTA1610.10112112552209276No Hit
GATGCTGGGCAGGTAGAATTAAAACAAACTGCTAGTATTACCAATTTTCACA1600.10049304399711081No Hit
TTTTAAGGGCACTTGTTCCTTAAATAGGGACCCGTATGAATGGCTCCACGAG1600.10049304399711081No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)