FastQCFastQC Report
Sat 31 Dec 2022
SRR5846635.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846635.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100510
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGACTATGATCTCGTATGC3950.3929957218187245No Hit
CTTATACACATCTCCGAGCCCACGAGACCGACTATGATCTCGTATGCCGTCT3110.30942194806486917No Hit
GAATTCGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1610.16018306636155605No Hit
AATCAGGGGTAAGTGGAAATGGTTTCTATATGGTAAAATGCCATGTAAGTCT1540.15321858521540146No Hit
CTGCCAGACCCTCCCCTCCCCGGGACTGTCTTCCTGTCTTTATGAAATCCCA1450.14426425231320267No Hit
ACTGCTGGGCAGCATATTGTAAGTGGCTACTAATTTTATTGGTATTTCAATG1430.1422744005571585No Hit
GACATAGGGAGAATTATTGCTCATGTAAATTTAAATATTGCTACTTATTTTT1430.1422744005571585No Hit
ATGCTGGGGTGTAACCAGGGAGGTGATGCAGCGTGACATAGCAGCCGGCGAC1430.1422744005571585No Hit
TCGCTAGGGGGGACTTAAGGAAACTAAAGCAACAACCAAATCCAAACTCAGA1410.14028454880111432No Hit
CCTCAGGGGCAGAACGGCATCAGTGTCTCTCCAGATTACCAGAGAAACCAGA1400.13928962292309222No Hit
TGATTTGGGACCTTAAAAATTAATGAAGTGGAAACTAGAAAAGATCTAAGAG1400.13928962292309222No Hit
GTTACTGGGCAGTTAGAAATGGGGTAAGGACTTAGCTTCTTTGTGGTAGTGA1380.13729977116704806No Hit
AATACAGGGGCGCCCTTGAAAGTTCTTGGATCTGCGGGTTATGGCCGGTCCC1380.13729977116704806No Hit
TCACTGGGGCTAGGCAGTAAATGGTTAGAGACGGTAAAGATCTGATTGTGAT1380.13729977116704806No Hit
CTGCTGGGTGTCCCAGTAGAGTTTGAGAAGTAGTTTCACGAGTTAGATGGAT1370.13630484528902598No Hit
ACTAGAGGGACCAACTGCCTGACTGCTTCCAAGTCAGCTCTATCCATCCCCA1360.13530991941100387No Hit
GTACAGGGGCAAAATATAAATATTCCAGAAAAGTTCTATATGATTTTTTTTT1360.13530991941100387No Hit
GCATCCGGGGTACCCAAACATCAGCAACAAGTCATGAGGTATAATGAACATA1350.1343149935329818No Hit
ACGACTGGGATTCCCCCAAATTCTGCTTTCTCTATTTTCTTTTTTATTTAAC1350.1343149935329818No Hit
GACAAAGGGGGGATTATGTTTCAACATGAGATTTGGATGGGGACAACATCCA1350.1343149935329818No Hit
AAATACGGGGGAATTAGAAGATGGGAAACATATTATTATTTAGTGGTCTGTC1340.1333200676549597No Hit
AAGCTAGGGCAGGTCACCAAATTGCCTCTAGTGAAAACCACATATCAGTTAG1340.1333200676549597No Hit
GGAGGGACACACACATACACAAATATTTGTAACATATTGTGACCAAATGGGC1330.13232514177693763No Hit
GGCATGGGGGACTTCAAACTCCACCGATTTTGCCTTAAAAAAAAGATTCATT1330.13232514177693763No Hit
TTATTTGGGATTACAGCCAGTAGAAATGATTTATTGATTTTTAAAAAGAAAA1330.13232514177693763No Hit
GCTACTGGGCACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATC1330.13232514177693763No Hit
CACCCACAGCTCAACAAGTCGCAGTCCCTGACAAATGCCTTCAGCTTCTCTG1330.13232514177693763No Hit
CAGTTAGGGAACTGAGAGTGCTGACCAAGCAAAGAAACATTTAGTTGATGGT1310.13033529002089345No Hit
TATGTGGGGCTCCCTCGGCCTCCCAAAGTACTGGGATTACAGGCATGAGCCA1300.12934036414287137No Hit
TTCGGGGAGTGCTACCCAGTTCCAGCTAATACTTCAGCATCTTTTCTTAACC1300.12934036414287137No Hit
AGCGCTGGGATTAGAGAAGCATTCCGTGTGTTTGATAAGGATGGCAATGGCT1290.12834543826484926No Hit
AACTAAGGGATAGGCGCAGTCATAGCACACTGCAGCCTAGAATTTCTGACCT1280.12735051238682718No Hit
CCAGCTGCTGAAGGAGATGACAAATGCTGACAGTCAGAACGAGGGGCTACTA1280.12735051238682718No Hit
TCATAGGGGCGATCTTATGCGGGGGCCTTTCGGCCGGAACCGCCATCTTCCA1280.12735051238682718No Hit
TCTCATGGGAGGATAAACTACTCATTGCTTTTTAAAGAAATATGTGTTTGCT1270.12635558650880507No Hit
TTCTTGGGCTACCACAGGCTGAGTTCTGGCCTGTGGTCTGGTCTTGCAGGCA1270.12635558650880507No Hit
CTTAGAGGGGTACTGGGAAATGGGAGCACAGTACTGGAAAATGGGAGCTCAC1260.12536066063078302No Hit
GTACTCGGGTGCTGGAGTTGACTTTCTTCTGTCCAAACAAACAAACAAAAAC1260.12536066063078302No Hit
TCAGGTAGGGACTACATCCATCCAACAAGGAGAAACAGCTTCAAAGGAGAGA1250.12436573475276091No Hit
CTGTTTGGGGAATCTGGCAAGCCTGGTACTGAAGTTAGGCCTGCTCTCCAGG1250.12436573475276091No Hit
GATATAGGGGACTTGTGAAATGAATTTGAAACCTGTCCAGGTTTGTCTTATA1250.12436573475276091No Hit
AGCCAGGGGCCTGATGAGAGAGAAGATGGTGCCAAGGCTGTCTTTGTATAAT1240.12337080887473884No Hit
TAAATAGGGACACGTGATCGGCCAACACTGAGTCTTACCTCGTTGTGGCGTC1230.12237588299671674No Hit
GACAAAGGGGAAGCAGCCATTCTTTCCAGTTTCAAACATATGGTCAGAGATT1230.12237588299671674No Hit
TAGGATGGGGAGTGTCAGGCTCTGAATCTCTCAAAATGTGCCGTCTTTGGTT1230.12237588299671674No Hit
TGCGGGGTAGGAAATGTTGGTAGTTACGAGTCACATCGTTGTCTACAAATCC1230.12237588299671674No Hit
TAATAGGGCACTCATTGATACCCTTTTGGATGGCTATGAAACAGCCCGCTAT1220.12138095711869466No Hit
CCAGCCATCCCAGCACCCCAGTAGACTCTAGCCTTCAAGCAAGACCAACCAA1220.12138095711869466No Hit
AATAAAGGGAACCCACGCTTGTAAAAGACATAAGAGTTTATATTAAGGACCA1220.12138095711869466No Hit
TTGCATGGGACTGTGCCTAGGAAGAGCTGGAGCTTGTGTTGTGACTGTAAAA1220.12138095711869466No Hit
ACTTCAGGGGATTTGAGATTTATAGTAAGTGGGAATACATACATGGATAAAC1220.12138095711869466No Hit
ATACTTGGGGATTTGAACATTATTTAAACAAATCTAGACTGAACATGAAAGA1210.12038603124067257No Hit
TTCATCCGGGTATTAAAAATATAACCACTTAAAAATATTAAAACCATTGTTA1210.12038603124067257No Hit
CTCTCCGGGCAAATACATAGAAGGAAGAGTGTGCATGCCTGTTATCTAGCAA1200.11939110536265049No Hit
CACGTTGGGCAGGGGCTTAGAAAATGAAAGCAAACACAAAACCTTGAGTGTG1200.11939110536265049No Hit
GAATCAGGGATAAGACAATCTCTCTAATACACCAGTCCTGCAAGCAGTTCAG1190.1183961794846284No Hit
TAGACGGGGGCGCGGCGGCACCGCGAAGGGAGGAGTGGCAACATGGCGTCTT1190.1183961794846284No Hit
AATAAAGGGCTGGATACATATAAAGCAAGATATATGGCTACAAATAAGTCTT1190.1183961794846284No Hit
TGACAGGGGATCCTTTGCTAGCAACAACATTCTTCTGTAACAACAAATAAAG1190.1183961794846284No Hit
AAATGGGGGGAGTCTTTAAGTTGAATTCACAAAAGTAATAGGTTAGAGCTGT1180.11740125360660632No Hit
GCCACTGGGCAACAATTTAGGAATGCTCAATGTGCTGTTGATTCTTTCAATC1180.11740125360660632No Hit
TTAAACGGGTACAACCAACTCTCAGAATTCAGACTTCCTGCAGCTGCCATAT1180.11740125360660632No Hit
AAATCAAGGGATTTGCTGCTGTTACTGAAGCTGGATCAATGTATATGGTTAT1180.11740125360660632No Hit
TACGTTGGGCTGAGCTTCACTATACAATACAGACAAAAGTGAGCTTTTCCAG1180.11740125360660632No Hit
TAAACTGGGAGGAGGAAAACAATGTTTTCAATCTTTTTTACTGAAAATTTGA1180.11740125360660632No Hit
CATTCAGGGAAGGTGTTTTAGCCTTTTTGGAAAAATTCTGGCAATATCTTAT1180.11740125360660632No Hit
GATTAGGGCAGCGGGGGATTGAAAATGATTCTTACCTTTAGCTGATGGATAA1170.11640632772858422No Hit
TGGATGGGGTAAATTAAAATCAGCAGATTGAGTTTTCAACATTGCAAAATCA1170.11640632772858422No Hit
GCAACAGGGAGGCTGAGAGAAGTGAAGAAGCTGCACAAGAAAAGTTGGAAGC1170.11640632772858422No Hit
CTCTAAGTGGGACTTCTGGAAATTAACCTCAATATTAGGTCAGCATGTGAAA1160.11541140185056215No Hit
TAGCAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1160.11541140185056215No Hit
AAACTCGGGTGAAAATGGGATGATATTGACAAATGGAGACTCCTACCTCAAT1160.11541140185056215No Hit
CTAAATGGGCCTCCCAAGCATTTTCCCATCCCATAAAGAATTAATGAAATGA1160.11541140185056215No Hit
GGCAAGGGTATGTACTATAATTATAGAATTGTTGAGCAAGGATTCTGAGCAA1150.11441647597254005No Hit
AGAACTGGGTAGTTGCCTCACTCACCTAATTCCATGGCATTTTTCAGTGATT1150.11441647597254005No Hit
GGACGTGGGGTGTGATATTTTTTTCTTCGCTTGTTTATTTATTCATTTATTT1150.11441647597254005No Hit
GCTAATGGGATATATCACGAACTTCAAGTGATTTCTGTCTCATTTCTTATTT1150.11441647597254005No Hit
TAAGTGGGGAATCATCTTTATATTTGGGGTTTTAATTACAGTTCCATTAGTG1150.11441647597254005No Hit
GCATCGGGGCACAACAGGAGTTGGGAAACTTTCTGAATAGGCAGCCAGTGGG1150.11441647597254005No Hit
CAGAAAGGGAGTGATTTTATATTATTTGTAGAAAGACATGCTGTATAGATTT1140.11342155009451795No Hit
TTGAAAGGGGAGCCCTTCAAGCTCAGGAAACCACGCTTCACTCGTGCTGACC1140.11342155009451795No Hit
ACTTAAGGGCTGGGTAAAAAGAACACAGGATCTCTGTATTATTTCTTACAAC1140.11342155009451795No Hit
CACATTGGGACCAATCCTAAATTATTAAATCATAAACTTGGCCTGGTCAAAG1140.11342155009451795No Hit
CGGTGTGGGACAGATGATGAGGCAGATTGAGATGAGGAAGAGGAGGATGAGT1130.11242662421649588No Hit
TATGCAGGGGGCTCGATCCGCATCTACAGCATGAGGTTCTGCCCGTTTGCTG1130.11242662421649588No Hit
TATCGCGGGCACTGTCTCTGTACTAGAAAAAGACACCTGATAATTAAAATAA1130.11242662421649588No Hit
GTAGTAGGGGAGTAAGTCTGTGTCTATTAGAGAAATTGAACTGTAATTCAAG1130.11242662421649588No Hit
TGCAGTGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAA1130.11242662421649588No Hit
TCTTCAGGGGGAGTACACTGTAAAATAATGATTAAAAAATCATGGTGTGGTA1130.11242662421649588No Hit
TAGTCGGGGAAGAAAAGGGGTAGAAAGCCCAAAAAGCTACAGTATGCAAAGC1120.11143169833847377No Hit
AGCTTGGGGGTAATTAAATGAGTTAACTTGAATAGAATTTCTGAAGAGCTTC1120.11143169833847377No Hit
CAACCAGGGGGGTGCGTTGATTGGGACATAGCCCCATCGAAAGTCAAGGAGC1120.11143169833847377No Hit
GCGCCTGGGCAAGCAGTGGTTAGAGGAAAACTGAGACGTGCACCCCCATGGG1120.11143169833847377No Hit
TTAGCAGGGCAGCTACCAAGATCAGTACCAGTGGTGCCGTCTTTTGTAGCCA1120.11143169833847377No Hit
TACTGAGGGTGCATCGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGAC1120.11143169833847377No Hit
GCAGGACGGGTAGCTTAGCCTATTCTTATACCAAGCAAAATTTATTGTATTT1120.11143169833847377No Hit
GTTGCTGGGTAACACACTTATTTTCATTGAATTTATGTATCTTTGTTATGTT1120.11143169833847377No Hit
CCCCCAATATCAATATCCCACACCGGAGTGGTAAGTTTGTTACAATCAGTGA1110.1104367724604517No Hit
CTTACTGGGGGGCAGGCATTCTCTATGCCTGCTAGGGAAGTGTGACTTGTTA1110.1104367724604517No Hit
TAAACCGGGATCTTGATTTCTACTCTATTTCTAACTGCATAGAGCATTTAAT1110.1104367724604517No Hit
ATCTATGGGGAAACAACTGTTTCCACACTTGCACCTGATCAAGAGCAGTGCT1110.1104367724604517No Hit
AACGCTGGGGAGAGCCGGAGGTCGGTGAGGGCCCGCGGAATCATCTATCTCG1110.1104367724604517No Hit
CACTCTGGGAAACCGACTTTCTGCATTTAACTACAAAAAAAGTTTATGTTTA1110.1104367724604517No Hit
ACAGATGGGTATTGATAATGTGGGACATATGTGTGATTCAATATATACATAT1100.1094418465824296No Hit
CAAGCTGGGTATCAACTAAGTTGGGCTCCCTGCTGTGAAATTCTCATAGTCA1100.1094418465824296No Hit
TGAATAGGGGAGTAAAACTGGTATAGTTTTTGCCCAACGGCACTTAAAACGA1100.1094418465824296No Hit
GCCAAGGGATACTTTTTTAAAAGGCAGAGCATTCTTTTTTCGGCAATTTTGA1100.1094418465824296No Hit
CAAGAAGGGATCCCGAACTAGGTTGAATGAGGTGCTGAGAGTGGGCATAATT1100.1094418465824296No Hit
TAGCATGGGTGCCAGCCTTGGCATCTGCATCTGTCTGATGCAGACAGATGTG1100.1094418465824296No Hit
GACGCGGGGTCCGATCTTCTTACGGGCAGCCACCATTTTGAGAACTATTATT1090.10844692070440752No Hit
GGTCACGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1090.10844692070440752No Hit
TCTAGTGGGTGTTGAACAACTCTAGTGAATTTTCAATTCAGATATTGTATTT1090.10844692070440752No Hit
TGTTTTGGGAAATAAGATTTTACAAAATGATTTTGGGCCTTTTTTTCTGTGT1080.10745199482638543No Hit
CACAACGGGATGTCTACTAATAGGAGAATGGATGAATAAGTGTCATTGTTGA1080.10745199482638543No Hit
ACAATGGGGATTGTAGCTTTATGCCATTAAGTTCTGAGGCAGTGTGCTATAC1080.10745199482638543No Hit
TCTCGCGGGATTGCAGCACTGTTCACAATAGCAAAGACTTAAAGCCAACCCA1080.10745199482638543No Hit
GCGCACGGAATATTAAATCAGTTTTGTTAAGGGGAAAACTCAATAGTTCTGT1070.10645706894836335No Hit
TCGTCTGGGGCTCCTGTTCGTGGCGGTGCGGCTGTGCAGGAGGAGCAGGGCG1070.10645706894836335No Hit
GAGGCAGGAGAATCGCTGGAACCCGGGGGGTGGAGATTGCAGTGAGCCGAGA1070.10645706894836335No Hit
ACTCAAGGGAGGTATGGATCTCTGTTGATTGACTCCAGTTGAAGGTGAGAAA1070.10645706894836335No Hit
CAGTCAGGGGTCCGGCCGGGCCGAGAGGTGGTTACATTCGTTGAAGGACACC1070.10645706894836335No Hit
AGTTATGGAAATTCAAACAATACTGAACATACTTACAATATCTAAAGATCTT1070.10645706894836335No Hit
TCCTGCCTTGGCCTCCCAAAGTGCTAGGATTATAGGCGTGAGCCATCACAGC1060.10546214307034125No Hit
GTATGGGGAGTCCATCAATGATATTTTTAAATGTAAAACACTTCTATTTATG1060.10546214307034125No Hit
TCTGATGGGAATGAAAATTGAGTATATTAAATGTGTGTGCTTGCATACACGA1060.10546214307034125No Hit
TACACAGGGGGAAGATGGAAGATGGTTGAATAGTGCTGAATATCAAGAGTGT1060.10546214307034125No Hit
CAAGCTAGGGTCAGAAAGAAAAAACAAAAAGAAAATTAGTGCTCTTAAGAAA1060.10546214307034125No Hit
GTTTTTGGGAAGTCAAGGACCCCAAATGGAGGGACCGGCTGGAGCTGTGGCA1060.10546214307034125No Hit
AGGTGGGGATCCAGCTATTGTTGTCTACAGAACACTAGCTTTAGATTTAAAG1060.10546214307034125No Hit
ACAAATGGGGCACTCTGATTGTGCCTTCATGGTAGACAATGAGGCCATCTAT1050.10446721719231918No Hit
TCACTGGGGGTTTTTCATATATCTGAAATATGCTAATTATGTTTTCTGTCTG1050.10446721719231918No Hit
GACCGGGTAGTACCACTTGCAAATGACCTAACCACAGGAAGCTTTTCAGATG1050.10446721719231918No Hit
AATGCAAGGGATGTTTATAAATGTAATTATCTATCATTAGATTTACGTAATT1050.10446721719231918No Hit
CTTACAGGGGTGGTAACATTGAATACAGTTGAATAAAATCGCTTACAAAACT1040.10347229131429708No Hit
CCCAAGGGTGCCAATTCACCACTTTCTACTTTGTGCTGGAACAGAACTGGCT1040.10347229131429708No Hit
GCATAGGGGGACAAGCTTTTGGGTGCTTTGTGGCTTGACAAAGTGATGTTCT1040.10347229131429708No Hit
CATCTGGGGAAGAAATGTACAATCTAGTGTTGGAGACAGGCAACAAACAAAG1040.10347229131429708No Hit
ACTATGGGGCACTGCCCTATGAAACATTTTAAAAATATTTTTTCAGTAGTTA1040.10347229131429708No Hit
TTTAGCGGGACGCTTGGGGGGAAATGAGCTTCACAGGCCCAAATAATGACAG1040.10347229131429708No Hit
CGAAATGGGATAACAATTGATTGCCATAGCAAGTTCCAAAGTGAATTTCAGC1030.102477365436275No Hit
TATGGGGGGTTCCAGCTCAACCAAGATAAAATGAATTTTTCCACACTGAGAA1030.102477365436275No Hit
CTGTTCGGGGTTGCCACCATCCTGTCTCGCCGTATTGCTGTTGAATATAGTG1030.102477365436275No Hit
ATTCGCGGGATGACAAGAAAATCTTAAAAACAGGAAAAACCCAAAGGAGAGA1030.102477365436275No Hit
TAAGTTTGGGGGGGGGACGGAGAGGAACAAGGATGGGGAGGTAGGAATGAGG1030.102477365436275No Hit
AAGGCAGGTGAGTTCTTTCTGGAAGGCTTGTGGGAAGTCTAAGTCCATTTTT1030.102477365436275No Hit
ATTGCTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1030.102477365436275No Hit
AGTGTAGGGCTAAAACAAGTGTTACATTGAGTCAAATATTTCCTAACTTAGT1020.10148243955825291No Hit
GTTAGAGGGACATTTTGTGCAGTGATTATTTTTTAAAGTCTTCTTTCATGTA1020.10148243955825291No Hit
AGACAAGGGCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACA1020.10148243955825291No Hit
GAAGCCAGAAGGGCTGAGTGTATTGTAAACTTATTCTTGCATGTTGCTGTCT1020.10148243955825291No Hit
AACAATAGGGTTCATGTGTCTTCTTTGGTAAAGTGTTTATTCACAGTTTTCG1020.10148243955825291No Hit
CTAGCTGGTGATACTAAGTCATTTTTAGGCCTTTTCATGAACACAGCTAGGG1020.10148243955825291No Hit
CCCAAAGGGGCCCTTTTCTTTGGCAAGATGGCGGAGTACGACTTGACTACTC1010.10048751368023083No Hit
CTACAGCGGGAATTGTGTGTTAGACATGATTCTTAGGTATTTATTGCATTTG1010.10048751368023083No Hit
GAACCAGGGGACGCTCTTCCGATCTGCTATAGGGAAGACTGTCAGTGCCAAC1010.10048751368023083No Hit
GTTCCTGGGAAACTGATCATCAGCAATTTCCCAGGAAGGAAAATAAAGAGTA1010.10048751368023083No Hit
ATTCGTGGGGGCAAGCAGTAAAAGCTCTCCTTTGCCACCAAGAGATTTGTGG1010.10048751368023083No Hit
CGCACTGGGTAATAAAATCACACTAGATTATCCATTGACTACACAAAAAGAA1010.10048751368023083No Hit
GATATAGGGCACCAAGCTGCGTGAAATGGGCAGAGAGAAATGTCACCAATAC1010.10048751368023083No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)