FastQCFastQC Report
Sat 31 Dec 2022
SRR5846633.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846633.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100712
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGACTATGATCTCGTATGC3820.379299388354913No Hit
CTTATACACATCTCCGAGCCCACGAGACCGACTATGATCTCGTATGCCGTCT3280.3256811502105012No Hit
CTTAGAGGGGTACTGGGAAATGGGAGCACAGTACTGGAAAATGGGAGCTCAC1600.15886885376122012No Hit
TCAGGTAGGGACTACATCCATCCAACAAGGAGAAACAGCTTCAAAGGAGAGA1550.153904202081182No Hit
AATCAGGGGTAAGTGGAAATGGTTTCTATATGGTAAAATGCCATGTAAGTCT1520.1509254110731591No Hit
CCTCAGGGGCAGAACGGCATCAGTGTCTCTCCAGATTACCAGAGAAACCAGA1490.14794662006513623No Hit
CAGTTAGGGAACTGAGAGTGCTGACCAAGCAAAGAAACATTTAGTTGATGGT1490.14794662006513623No Hit
GCATCCGGGGTACCCAAACATCAGCAACAAGTCATGAGGTATAATGAACATA1460.14496782905711336No Hit
CTGCCAGACCCTCCCCTCCCCGGGACTGTCTTCCTGTCTTTATGAAATCCCA1460.14496782905711336No Hit
CATTCAGGGAAGGTGTTTTAGCCTTTTTGGAAAAATTCTGGCAATATCTTAT1450.14397489872110572No Hit
GACATAGGGAGAATTATTGCTCATGTAAATTTAAATATTGCTACTTATTTTT1430.14198903804909047No Hit
CCACCCTACAAGTACTGAAAAGGCACAGGTAACCACTTTTTATAATAAATTC1410.14000317737707524No Hit
GGAGGGACACACACATACACAAATATTTGTAACATATTGTGACCAAATGGGC1370.13603145603304473No Hit
CGCGCGGGGTATGTCAAAGCAACTTCTGATAATATACCTGAATTTGTAGTTG1370.13603145603304473No Hit
TTGAAAGGGGAGCCCTTCAAGCTCAGGAAACCACGCTTCACTCGTGCTGACC1360.1350385256970371No Hit
GCGCACGGAATATTAAATCAGTTTTGTTAAGGGGAAAACTCAATAGTTCTGT1350.13404559536102947No Hit
ATGCTGGGGTGTAACCAGGGAGGTGATGCAGCGTGACATAGCAGCCGGCGAC1350.13404559536102947No Hit
TGATTTGGGACCTTAAAAATTAATGAAGTGGAAACTAGAAAAGATCTAAGAG1350.13404559536102947No Hit
TCACTGGGGCTAGGCAGTAAATGGTTAGAGACGGTAAAGATCTGATTGTGAT1350.13404559536102947No Hit
CAGAAAGGGAGTGATTTTATATTATTTGTAGAAAGACATGCTGTATAGATTT1330.13205973468901422No Hit
TTTGCAGGGTTTGAAACCAACTACTGTAGAAGATGAGGAATCTATTGTAATT1330.13205973468901422No Hit
GAATTCGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1330.13205973468901422No Hit
CAGTCAGGGGTCCGGCCGGGCCGAGAGGTGGTTACATTCGTTGAAGGACACC1320.1310668043530066No Hit
TAACTAAGGGTATAAGCTCTGGGATTCTTTTTGGAGCAATACCTACAAAGTC1310.13007387401699896No Hit
GATATAGGGGACTTGTGAAATGAATTTGAAACCTGTCCAGGTTTGTCTTATA1310.13007387401699896No Hit
AATAAAGGGCTGGATACATATAAAGCAAGATATATGGCTACAAATAAGTCTT1290.1280880133449837No Hit
GTTACTGGGCAGTTAGAAATGGGGTAAGGACTTAGCTTCTTTGTGGTAGTGA1280.1270950830089761No Hit
CTTACAGGGGTGGTAACATTGAATACAGTTGAATAAAATCGCTTACAAAACT1270.12610215267296845No Hit
GCCACTGGGCAACAATTTAGGAATGCTCAATGTGCTGTTGATTCTTTCAATC1270.12610215267296845No Hit
GCTACTGGGCACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATC1270.12610215267296845No Hit
ACTGCTGGGCAGCATATTGTAAGTGGCTACTAATTTTATTGGTATTTCAATG1260.12510922233696084No Hit
TTCTTGGGCTACCACAGGCTGAGTTCTGGCCTGTGGTCTGGTCTTGCAGGCA1260.12510922233696084No Hit
AAATACGGGGGAATTAGAAGATGGGAAACATATTATTATTTAGTGGTCTGTC1250.12411629200095321No Hit
TGTGACGGGTATTGCACCATTTTTGTATTAACGTAGAAACTAAGCATCAGAA1250.12411629200095321No Hit
GTTTTGGGGGATTACGAAAATATAGTACCCATGAGCATTTCATGTTAGAAAC1250.12411629200095321No Hit
GATTAGGGCAGCGGGGGATTGAAAATGATTCTTACCTTTAGCTGATGGATAA1250.12411629200095321No Hit
AGACACGGGCAAACAGGTTGAAAAAAATGATGACGACCAAAAGATTGAACAA1250.12411629200095321No Hit
TATCGCGGGCACTGTCTCTGTACTAGAAAAAGACACCTGATAATTAAAATAA1250.12411629200095321No Hit
CGCACTGGGTAATAAAATCACACTAGATTATCCATTGACTACACAAAAAGAA1250.12411629200095321No Hit
TTCATCCGGGTATTAAAAATATAACCACTTAAAAATATTAAAACCATTGTTA1250.12411629200095321No Hit
CGGTGTGGGACAGATGATGAGGCAGATTGAGATGAGGAAGAGGAGGATGAGT1240.12312336166494559No Hit
CCAGCTGCTGAAGGAGATGACAAATGCTGACAGTCAGAACGAGGGGCTACTA1240.12312336166494559No Hit
ATCTATGGGGAAACAACTGTTTCCACACTTGCACCTGATCAAGAGCAGTGCT1240.12312336166494559No Hit
TGCGGGGTAGGAAATGTTGGTAGTTACGAGTCACATCGTTGTCTACAAATCC1240.12312336166494559No Hit
CACCCACAGCTCAACAAGTCGCAGTCCCTGACAAATGCCTTCAGCTTCTCTG1240.12312336166494559No Hit
TAGTCGGGGAAGAAAAGGGGTAGAAAGCCCAAAAAGCTACAGTATGCAAAGC1230.12213043132893796No Hit
AGCTTGGGGGTAATTAAATGAGTTAACTTGAATAGAATTTCTGAAGAGCTTC1230.12213043132893796No Hit
CAACCAGGGGGGTGCGTTGATTGGGACATAGCCCCATCGAAAGTCAAGGAGC1230.12213043132893796No Hit
AGGTGGGGATCCAGCTATTGTTGTCTACAGAACACTAGCTTTAGATTTAAAG1230.12213043132893796No Hit
GGCATGGGGGACTTCAAACTCCACCGATTTTGCCTTAAAAAAAAGATTCATT1210.12014457065692272No Hit
TTATTTGGGATTACAGCCAGTAGAAATGATTTATTGATTTTTAAAAAGAAAA1210.12014457065692272No Hit
GACGCGGGGTCCGATCTTCTTACGGGCAGCCACCATTTTGAGAACTATTATT1210.12014457065692272No Hit
CAGCACGGGACCTTTCATTTTCCCTCTGTTACCAAATTTCAGCTCTTAGGAG1200.11915164032091509No Hit
TATGTGGGGCTCCCTCGGCCTCCCAAAGTACTGGGATTACAGGCATGAGCCA1200.11915164032091509No Hit
ACGCTTGGGGTAGATGAAGTCTCATAGGCGGCCACCCTTAGAGGCAATAGAT1200.11915164032091509No Hit
GCATCGGGGCACAACAGGAGTTGGGAAACTTTCTGAATAGGCAGCCAGTGGG1200.11915164032091509No Hit
GTGCTCGGGGGAGGACTTCATCAACAACATTAACAGTGTCTTGGAGTCCTTG1190.11815870998490745No Hit
AATAAAGGGAACCCACGCTTGTAAAAGACATAAGAGTTTATATTAAGGACCA1190.11815870998490745No Hit
GAGGCAGGAGAATCGCTGGAACCCGGGGGGTGGAGATTGCAGTGAGCCGAGA1180.11716577964889982No Hit
ATTGCTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1180.11716577964889982No Hit
TACGCCGGGGACACTTTGACAAGCACACTGGATGGGCAAAAGAGTCAACTAC1180.11716577964889982No Hit
TAGACGGGGGCGCGGCGGCACCGCGAAGGGAGGAGTGGCAACATGGCGTCTT1170.1161728493128922No Hit
AAATCAAGGGATTTGCTGCTGTTACTGAAGCTGGATCAATGTATATGGTTAT1170.1161728493128922No Hit
TTAGCAGGGCAGCTACCAAGATCAGTACCAGTGGTGCCGTCTTTTGTAGCCA1160.11517991897688458No Hit
TGAATAGGGGAGTAAAACTGGTATAGTTTTTGCCCAACGGCACTTAAAACGA1160.11517991897688458No Hit
TGCTTTAAAGTATAGTAACACTAACATCTAGGTAACATGCAGCTAGTATTGA1160.11517991897688458No Hit
CTGTTTGGGGAATCTGGCAAGCCTGGTACTGAAGTTAGGCCTGCTCTCCAGG1160.11517991897688458No Hit
AACAATAGGGTTCATGTGTCTTCTTTGGTAAAGTGTTTATTCACAGTTTTCG1160.11517991897688458No Hit
GGCAAGGGTATGTACTATAATTATAGAATTGTTGAGCAAGGATTCTGAGCAA1150.11418698864087695No Hit
CAATCAGGGATTGTTTTTTTCTGCAGATTCTCACAGTTGTCAATGATAATGT1150.11418698864087695No Hit
TTTAGCGGGACGCTTGGGGGGAAATGAGCTTCACAGGCCCAAATAATGACAG1150.11418698864087695No Hit
ATGCCTGGGGTCTGCAGAGAGAATTTTGATACAAAGAGGAATGAACTAACAA1140.11319405830486932No Hit
TCACATGGGAGGTGCCTTCAGGTTGACATAGCTAAATGACCCAAATTGTCTG1140.11319405830486932No Hit
TGGATGGGGTAAATTAAAATCAGCAGATTGAGTTTTCAACATTGCAAAATCA1140.11319405830486932No Hit
CACTCTGGGAAACCGACTTTCTGCATTTAACTACAAAAAAAGTTTATGTTTA1140.11319405830486932No Hit
CGTTTGGGGGAAAGAAAAAACTGAAAACTGTCCTATTAACCAACATTGTAAA1140.11319405830486932No Hit
TCTAGTGGGTGTTGAACAACTCTAGTGAATTTTCAATTCAGATATTGTATTT1140.11319405830486932No Hit
AGCGCTGGGATTAGAGAAGCATTCCGTGTGTTTGATAAGGATGGCAATGGCT1130.11220112796886171No Hit
ACAAATGGGGCACTCTGATTGTGCCTTCATGGTAGACAATGAGGCCATCTAT1130.11220112796886171No Hit
CAACGTGGGAATTAGGCATTGGTCAGGGGTGAATGGCTCTTTTCACAGAGAG1130.11220112796886171No Hit
TAAATAGGGACACGTGATCGGCCAACACTGAGTCTTACCTCGTTGTGGCGTC1130.11220112796886171No Hit
GCTAATGGGATATATCACGAACTTCAAGTGATTTCTGTCTCATTTCTTATTT1130.11220112796886171No Hit
TCTCATGGGAGGATAAACTACTCATTGCTTTTTAAAGAAATATGTGTTTGCT1130.11220112796886171No Hit
CCCCCAATATCAATATCCCACACCGGAGTGGTAAGTTTGTTACAATCAGTGA1120.11120819763285408No Hit
TTTGGCTGGGCATATTTTTCAGGTAGTACCACTGATCAAAACAATACAATGA1120.11120819763285408No Hit
TAGCATGGGTGCCAGCCTTGGCATCTGCATCTGTCTGATGCAGACAGATGTG1120.11120819763285408No Hit
GACCGGGTAGTACCACTTGCAAATGACCTAACCACAGGAAGCTTTTCAGATG1110.11021526729684646No Hit
CAAATAGGGCAGATCCACACAACTTAAAGATCTGCTGCCGAGTGAATGGGGA1100.10922233696083881No Hit
TAATAGGGCACTCATTGATACCCTTTTGGATGGCTATGAAACAGCCCGCTAT1100.10922233696083881No Hit
TTAAACGGGTACAACCAACTCTCAGAATTCAGACTTCCTGCAGCTGCCATAT1100.10922233696083881No Hit
CTGTTCGGGGTTGCCACCATCCTGTCTCGCCGTATTGCTGTTGAATATAGTG1100.10922233696083881No Hit
TACTGAGGGTGCATCGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGAC1100.10922233696083881No Hit
AAGCTAGGGCAGGTCACCAAATTGCCTCTAGTGAAAACCACATATCAGTTAG1100.10922233696083881No Hit
GAATCAGGGATAAGACAATCTCTCTAATACACCAGTCCTGCAAGCAGTTCAG1090.10822940662483121No Hit
GGGATAGGGATAGCTGCATTTCTTTAGTTATTTTGAGCCCCAAATATTTCCT1090.10822940662483121No Hit
TCAACTGGGTTTGCTCTACATGTAACAACACCTCACTTAGCTCGTGAGTTAA1090.10822940662483121No Hit
ATTCGTGGGGGCAAGCAGTAAAAGCTCTCCTTTGCCACCAAGAGATTTGTGG1090.10822940662483121No Hit
GCGCCTGGGCAAGCAGTGGTTAGAGGAAAACTGAGACGTGCACCCCCATGGG1090.10822940662483121No Hit
AAAGCTAGGCATACAATGTCATTGAATCCTCCCAGTCATGCTCTGAGGTAAG1090.10822940662483121No Hit
GCTGCAGGGGGCACTGTACATCTAAAAATATTACAGTAGAATCTGAGTGTAA1090.10822940662483121No Hit
GCCAAGGGATACTTTTTTAAAAGGCAGAGCATTCTTTTTTCGGCAATTTTGA1090.10822940662483121No Hit
TCATAGGGGCGATCTTATGCGGGGGCCTTTCGGCCGGAACCGCCATCTTCCA1090.10822940662483121No Hit
GAACAGGGTGGAAAGTTAAAGGGAGAAAGAAAAGAACAAAGTGAAGAAATTA1090.10822940662483121No Hit
AGTCATGGGGATCTAAGGATGGGGATGCCTGTCAGGTCCAGCCTTACCTGTG1090.10822940662483121No Hit
ACTTCAGGGGATTTGAGATTTATAGTAAGTGGGAATACATACATGGATAAAC1090.10822940662483121No Hit
ATGATTGGGGGCATAGATTTAATAAAGAACACCTGAATTAAATACCCTTTGA1080.10723647628882357No Hit
CACAACGGGATGTCTACTAATAGGAGAATGGATGAATAAGTGTCATTGTTGA1080.10723647628882357No Hit
AGAACTGGGTAGTTGCCTCACTCACCTAATTCCATGGCATTTTTCAGTGATT1080.10723647628882357No Hit
ACGACTGGGATTCCCCCAAATTCTGCTTTCTCTATTTTCTTTTTTATTTAAC1080.10723647628882357No Hit
GACAAAGGGGGGATTATGTTTCAACATGAGATTTGGATGGGGACAACATCCA1080.10723647628882357No Hit
ATTTTTGGGCACTAAACAAAAATACCCACTCAGTCTGTGTTAAATTGTATGT1070.10624354595281595No Hit
AACTAAGGGATAGGCGCAGTCATAGCACACTGCAGCCTAGAATTTCTGACCT1070.10624354595281595No Hit
GACAAAGGGGAAGCAGCCATTCTTTCCAGTTTCAAACATATGGTCAGAGATT1070.10624354595281595No Hit
TACGTTGGGCTGAGCTTCACTATACAATACAGACAAAAGTGAGCTTTTCCAG1070.10624354595281595No Hit
TCGAGGGGGAGAAGAAGGTATTTGGAGCCTATAATTCCATTGAGGGTTATGA1070.10624354595281595No Hit
TGCAGTGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAA1070.10624354595281595No Hit
TCGTAGGGGGTCATATGTATTTTTTGAATTAGCTGCTCAAATCCCTTGCCCA1060.10525061561680832No Hit
GTAGAGGGGTAGGGGGGCAGTCACATGCTTCGTAGTGGATTGGGCTGGATTC1060.10525061561680832No Hit
TGATATGGGCACCTTTACTTTAGAAGCACAGTAAATATCCCAAACTGTGATG1060.10525061561680832No Hit
TGCACAGGGGACGCTCTTCCGACCTAATCAAGGGATTCATTCATTAGGAACA1060.10525061561680832No Hit
CAAGCTGGGTATCAACTAAGTTGGGCTCCCTGCTGTGAAATTCTCATAGTCA1060.10525061561680832No Hit
GTAGTCGGGGCCCTGCTCTGCGGCCTCCTTTGTCCCATAACCTGAAGTTAAG1060.10525061561680832No Hit
AAACTCGGGTGAAAATGGGATGATATTGACAAATGGAGACTCCTACCTCAAT1060.10525061561680832No Hit
GCTTGAGGGTTCTGGTATCCTTTAAGAAAAGTTAAGACTATTATTTCCTTCC1050.1042576852808007No Hit
CTGCTGGGTGTCCCAGTAGAGTTTGAGAAGTAGTTTCACGAGTTAGATGGAT1050.1042576852808007No Hit
CTTACTGGGGGGCAGGCATTCTCTATGCCTGCTAGGGAAGTGTGACTTGTTA1050.1042576852808007No Hit
GCATAGGGGGACAAGCTTTTGGGTGCTTTGTGGCTTGACAAAGTGATGTTCT1050.1042576852808007No Hit
CGTCAAGGGTGAAGAGACAGGCTATCTGGTTAATGTTACATAGGAATTTAAT1050.1042576852808007No Hit
CTCTAAGTGGGACTTCTGGAAATTAACCTCAATATTAGGTCAGCATGTGAAA1040.10326475494479308No Hit
AAAGAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1040.10326475494479308No Hit
GTATGGGGAGTCCATCAATGATATTTTTAAATGTAAAACACTTCTATTTATG1040.10326475494479308No Hit
CTACAGCGGGAATTGTGTGTTAGACATGATTCTTAGGTATTTATTGCATTTG1040.10326475494479308No Hit
TATGGGGGGTTCCAGCTCAACCAAGATAAAATGAATTTTTCCACACTGAGAA1040.10326475494479308No Hit
GAGCCGGGATATGAAAGGATTCATCTGATATTTCATGAAAATCTTAGCAAAG1040.10326475494479308No Hit
GTATTTGGGCAGTGATTTAGTTATCCTTCTCTTCTTGGTGTCGAGAGAGGTA1040.10326475494479308No Hit
TCTTCAGGGGGAGTACACTGTAAAATAATGATTAAAAAATCATGGTGTGGTA1040.10326475494479308No Hit
CTCGCGGGGGTATTAAGGCCTTTGCAATAACAATAATAATAGCTATTATTTC1030.10227182460878545No Hit
CTAGCTGGTGATACTAAGTCATTTTTAGGCCTTTTCATGAACACAGCTAGGG1030.10227182460878545No Hit
TACCAGGGGAACCTATTTGTAAACAAAATAGAACCTGGCCAGAAATAATGAA1020.10127889427277781No Hit
TAGGATGGGGAGTGTCAGGCTCTGAATCTCTCAAAATGTGCCGTCTTTGGTT1020.10127889427277781No Hit
AAGGCAGGTGAGTTCTTTCTGGAAGGCTTGTGGGAAGTCTAAGTCCATTTTT1020.10127889427277781No Hit
GAAGCCAGAAGGGCTGAGTGTATTGTAAACTTATTCTTGCATGTTGCTGTCT1020.10127889427277781No Hit
AGTGTAGGGCTAAAACAAGTGTTACATTGAGTCAAATATTTCCTAACTTAGT1010.10028596393677021No Hit
ATAGATGGGGAGCAAAGGAAGCCTTGCGACACCATGAAAGTTGGTGGAAACC1010.10028596393677021No Hit
CAAGCTAGGGTCAGAAAGAAAAAACAAAAAGAAAATTAGTGCTCTTAAGAAA1010.10028596393677021No Hit
ACTCATGGGACCTGCTGACCTCTGTGTCCCTAGACTGAATGAAGGGGACCAA1010.10028596393677021No Hit
TAAGTGGGGAATCATCTTTATATTTGGGGTTTTAATTACAGTTCCATTAGTG1010.10028596393677021No Hit
ACTATGGGGCACTGCCCTATGAAACATTTTAAAAATATTTTTTCAGTAGTTA1010.10028596393677021No Hit
AAGCTCGGGGCAAACTGAATCCAAGAGCACGTTAAAATAATCATGCATCATG1010.10028596393677021No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)