FastQCFastQC Report
Sat 31 Dec 2022
SRR5846614.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846614.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9227
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGACTATGATCTCGTATGC490.5310501788230194No Hit
CTTATACACATCTCCGAGCCCACGAGACCGACTATGATCTCGTATGCCGTCT440.47686138506556847No Hit
CCGTCCGGGAAGCTAAGATGCCTTCTAACTTCATAAGCAAACCTTTAACTAA160.17340414002384308No Hit
TGTGGCGGGATAAATATAGGAATTACAAATGTTGATGTTAAAGCTATACCAG150.16256638127235287No Hit
GGCGTAGGGTCTACACGTGGGGCCGCCATCTTGGTTCCGCGTTCCCTGCACA140.15172862252086267No Hit
TGTGACGGGATTGTCAATTAGGCATTTGGATGTAGGCATTTGGGAATTTAGA140.15172862252086267No Hit
TTCAATGGGATGATATTAACCCAGAAAGTTTAGGCATACCAGGTCCTTGAAA140.15172862252086267No Hit
GCAAAGGGACAGCACCTGGTAGGTTACAAGCATTTTGAGCAGATATACAAAA140.15172862252086267No Hit
AGATGTGGGGAGCACTGAAGTCCTTTAAAAGCCATGTTGCCTGTTTTATGGT140.15172862252086267No Hit
ACGCATGGGTGGCGAGCGTCTGCACGACCAGGAGAGAGGCTGGTTCCCCAGC140.15172862252086267No Hit
CAATATGGGTGAGTTATGAATAGGAGTTGTAAATCACCCCAAAAAAACAAGA130.14089086376937252No Hit
TTAGCAGGGGACCAAACCATTTGTATCTGGCATCACTTACTAACACACGACA130.14089086376937252No Hit
TTTGCTGGGAATATAAAAATTCTCTTTCATTTCTTTTTGCCAGCAATCAAAA130.14089086376937252No Hit
ACAATAGGGAGTTCTAGAAGTTCCGCTGCAAGGCCAGGAAAGCTTGAGAAAG130.14089086376937252No Hit
GCGAGTTGGGGTTGACAGTGTTGAAGCAGGGTGAATAACTAGGGCATATATT130.14089086376937252No Hit
TGATCTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC130.14089086376937252No Hit
GTTACTGGGCTGGAGCTCCTTTCCCTTGACTTAAGTTGATTCAGCTGGCAGC120.1300531050178823No Hit
TCGCGCGGGCAAGAAACCTTGGGCCACCGCTTTGTACATAGCACCGTGCTAG120.1300531050178823No Hit
CATTGCGGGAAGGCAGCGGTGCTTAATCAATATTTCCTGTGCTCACCAGAGG120.1300531050178823No Hit
GATATAGGGGATACTTAGTGAGGAGAGAAAAGGAAAACACAGTTTTTTTGCA120.1300531050178823No Hit
TCCCTAGGGATCGATAATATACAATAATGACTTTTTAAAAAATGAGTCTGAT120.1300531050178823No Hit
TAGCAGGGGACCTTTAGTACATCTCTCTACCAAAAAATCTTCCTATCTCTAG120.1300531050178823No Hit
TTGCTAGGGAGTTCCAAGTTTCAAAGAAATGTCAACATTTTATTCCATTCAA120.1300531050178823No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGACTATG120.1300531050178823No Hit
CTGCTGGGGAAGTAAGCATTTCTTCCGTTATTGCATTTTCCTGATTACAAGT120.1300531050178823No Hit
AGTTTAGGGGTGGAAGGTGTCAATTTGGTTTAGAATGCTGCGCTTACCTTCC120.1300531050178823No Hit
TGAAAAGGGTCCTATGGTAATGATGCTTCTTCCTGTAATACTTCCTGAAAGA120.1300531050178823No Hit
GTCGAGGGGTCTGCGAAGGGAAGATGGAAGGAGACCATGAACCGAGGGGTTT120.1300531050178823No Hit
TCCGCCGGGGGTTCTTAGTCGAGAGGGACTCTACTGAGAGGGGCCTCCAACC120.1300531050178823No Hit
GCTGGGGCATTGTGTTATATAAAAATATTTAAGTGTTATGGCATCTGAGTAA120.1300531050178823No Hit
GATCAAGGGTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAGTGGT110.11921534626639212No Hit
TCATCGAGGGAGATTTTAAAAATGGAGGTGAAATAGACATTCTCAGATGAAG110.11921534626639212No Hit
GTGATTGGGCAGGGAGGGGAACAGGAGCCAGACATGACAGGGACTTGGCCCT110.11921534626639212No Hit
GCTTCTGGGTCTGATGAAGGGGTTGTTTGTTAAAGATGTTCCACTGAAAGCA110.11921534626639212No Hit
AGGTTAGGGATAAGGAACAATTGGATAAATTTAACCACCTAATAATTTAAAA110.11921534626639212No Hit
CACATTGGGCTTCTCTCTCCAGGTAATTAAACAATTCTGATTGAGAAACCTG110.11921534626639212No Hit
GTCATTGGGAGAGTGCAGTGGTATGAGCTCAGCTCACTGCAACCTCCACCTC110.11921534626639212No Hit
TTAGTGGGACGGGCTTTCCATGCAGATACATCAATGGCCAAAGGAAGATAAG110.11921534626639212No Hit
TATATAGGGTGACTATGGTAAGTTTGAATCTCTCCTTGCCAAACAACATTAT110.11921534626639212No Hit
GTATTTGGGATCTTTTGAAACTGCATAAAAGTGAAATAATTATGAGTTTTGG110.11921534626639212No Hit
GGTGTAGGGTTGGTATGGTTAGTCTTTTAAATTTTTGTGATTCTAATAAGTG110.11921534626639212No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGACTATGACTGTCTCTTA110.11921534626639212No Hit
CGTTCTGGGATAAATGGAGGCTTTTTGTATTTCACACATCATTTAACTAGTG110.11921534626639212No Hit
TGCACCGGGCAGAGACATTCCTCCGAGCCGGGGAGTTTCTTTCCTGCCTTCT110.11921534626639212No Hit
GTCTTAGGGTCTACAAATGCAAGTAAAATTTCCTGTAACAGCATTTCTCCTG100.10837758751490191No Hit
TATCCAGGGGAAACTGAAACTCGGAGAAGTTAAATGATTCCCCCACGATCCC100.10837758751490191No Hit
TCAATCGGGTGCATGTGGGCTCCTCAGTTATTGAGTTTTTGTGATCCTATCT100.10837758751490191No Hit
TAATAGGGGTTTCTTTCTGTCCACAATGATTTGTGTCATAGACATAATTGAT100.10837758751490191No Hit
TATCTAGGGTGGAAGGTGTCAATTTGGTTTAGAATGCTGCGCTTACCTTCCC100.10837758751490191No Hit
CTGCTTGGGGAGGCAATCTTAATCATCACAGCAGGAAAACAGGCACAAATCC100.10837758751490191No Hit
AACAATGGGAGTCTGTAAAAGTGCATATTGGCTTCTCACCAAGACTGAGAGT100.10837758751490191No Hit
GAGTGAGGGAATGCTTGAAAATGTTATTAAGAAGGAGACTTACCATGCAGAC100.10837758751490191No Hit
TCAGGTGGGGACTGGGATGTTGTTTGCCTTGTATGATCAGAAAAAAATTAAG100.10837758751490191No Hit
CGTAATGGGGTGTGTGCACATCCCTGGAGTCACTCTGTATGTAGAAATGTCT100.10837758751490191No Hit
TCGCCTGGGGTGTGTGTGTGTTCAAAAATGTCTATGAAAATGAAAACCCACA100.10837758751490191No Hit
GATAATGGGTGTTTTAAAATTACACTTTTTCTTAACCTGTCTAGCTGTCGGA100.10837758751490191No Hit
GTTCAAGGGGTATTTTTTTAAAAATAATCTTTTTTCTAGCATCGGAGAAGTT100.10837758751490191No Hit
ATGACAGGGTGCATCAGAGATTTAATTTTTTTTTAATTTTTAATTTTTTTAA100.10837758751490191No Hit
TAGCCAGGGTGCTAAACTTGCTGCTGCAGTCCTAAGAATTTAAACACTCAAA100.10837758751490191No Hit
AGTATAGGGGTGAACATTTCAATTATTAATGATAACAATGCATCAGTATATA100.10837758751490191No Hit
GCTTATGGGGAGAGAAAAAATTATAAAAAAACCATGGTAAGAGTGTGATAAC100.10837758751490191No Hit
AGCTAAGGGCGATCTAGAGGCGGGAGACTGCTGTGCTAGCAGTGAGCGAGGC100.10837758751490191No Hit
GATGCAGGGCTGGAGCTTGCGCAGGAGCTGTAGCTGCCGCAGCCACAACCCC100.10837758751490191No Hit
GAACTGGGGATTCTCTCAGAGCAAGAACTACTCACCTCTGTAGGAGGGCATT100.10837758751490191No Hit
GCTTAAGGGATGTTGGTCGGGCTGGTCTCAAACTCCTGACCTCTTGATCCGC100.10837758751490191No Hit
CGCGAGGGACCACCACTGGATCCATCTTCCACATTGTCTGAGCAATGATTTT100.10837758751490191No Hit
AAAATGGGGGTGACTGGGAAGGAGAACTTATTCCTTACCAGGAAACTGGAAG100.10837758751490191No Hit
GTTATGGGAGCGCCGGCTATGCCCCTGTATTGGATTGCCACACGGCTCACAT100.10837758751490191No Hit
CAACAGGGTGAGACTGTGTCTCTAAATAAATAAATGGGGTGGCACAGATGCA100.10837758751490191No Hit
AAAGCTGGGAATCTTCTTTCTTTATGCAGTTGAACATATCCAGTTTGGAAAT100.10837758751490191No Hit
GAATGTGGGATAGCCGTTTATTTGCCATTCTTTTATAGACTTTAGCAGATTA100.10837758751490191No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)