FastQCFastQC Report
Sat 31 Dec 2022
SRR5846613.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846613.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9268
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGACTATGATCTCGTATGCCGTCT470.5071212775140267No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGACTATGATCTCGTATGC470.5071212775140267No Hit
GCGAGTTGGGGTTGACAGTGTTGAAGCAGGGTGAATAACTAGGGCATATATT200.21579628830384118No Hit
CAATATGGGTGAGTTATGAATAGGAGTTGTAAATCACCCCAAAAAAACAAGA170.183426845058265No Hit
CTGCTGGGGAAGTAAGCATTTCTTCCGTTATTGCATTTTCCTGATTACAAGT170.183426845058265No Hit
GATCACGGGTCTTTCTGTGGTGAGAATGGTTAAAATCTACTCTTTTCACAAT160.17263703064307295No Hit
CGAGCTGGGGTTTGTGTTTTTTAAATAACAGCTTTATTGGTACATAATTCAC150.16184721622788087No Hit
ATAACTGGGATCTTCCTATCCCTAAAGAGGAGGGAGAAAGTACTTATTTAGC150.16184721622788087No Hit
TCGCGCGGGCAAGAAACCTTGGGCCACCGCTTTGTACATAGCACCGTGCTAG140.1510574018126888No Hit
TAGTCAGGGGAATTAGCTAAACAAAGAAATCATGGCGGATTGTTTACATTTG140.1510574018126888No Hit
CGCACGGGGGTGGTTTAATTTTTTTTTGAGACAGAGTCTTGCTGTATTGCCC140.1510574018126888No Hit
ACGCATGGGTGGCGAGCGTCTGCACGACCAGGAGAGAGGCTGGTTCCCCAGC140.1510574018126888No Hit
TATTAGGGGCAGGCACCAGGCCTGGTCCAGGAGGAGCTGTGGACACAGTCGT140.1510574018126888No Hit
GCTCTGGGGCAATGATATCTCTGAGTTGAGGAGACAGAAAATGATATTCAGA130.14026758739749676No Hit
TAGCCAGGGTGCTAAACTTGCTGCTGCAGTCCTAAGAATTTAAACACTCAAA130.14026758739749676No Hit
GGAGAGGGGCAATTTCACGTGGATAGCTCTTCATGTCACTCAAAAGATCAAA130.14026758739749676No Hit
AGCAGTGGGACTCCTTAATTTGTTATGTTTCACTGTTATCTGTATTTTTGAG130.14026758739749676No Hit
GGTTATGGGGGAGGCGAATTAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTG120.1294777729823047No Hit
AGCTTGGGGAGAAATACTTTCATTAAAAACCTATTGGTAGAAATTTGCCAGT120.1294777729823047No Hit
TTGCTAGGGAGTTCCAAGTTTCAAAGAAATGTCAACATTTTATTCCATTCAA120.1294777729823047No Hit
TTCAATGGGATGATATTAACCCAGAAAGTTTAGGCATACCAGGTCCTTGAAA120.1294777729823047No Hit
GTCTCGGGGGGCTGAAAACGGAGAGGGAATGGGAGACTTGATATTTAAGCAG120.1294777729823047No Hit
AGTTGGGAAGTCAAGGGCCGAGGCGAAACGAATGAAGGTGGCAAAGAATGCA120.1294777729823047No Hit
CAGGCAGGGTGGCTTAGAAATGTGCCAGTGTGTTCAAAACATTTACACCAAT120.1294777729823047No Hit
TTTTAAGGGATAGTAGATTTGTTTGTTTTAATACCTTCAGTTAATGTCCAGA120.1294777729823047No Hit
ACTATAGGGAACTCCATAGCAGCCAACATGAATAAGTTTTCACTGGAACCCA120.1294777729823047No Hit
AACGCAGGGAGACTACAAGGATAGGCCCAGGAGTAATGGAGTCCAAAGAGAA110.11868795856711264No Hit
GTCTTAGGGTCTACAAATGCAAGTAAAATTTCCTGTAACAGCATTTCTCCTG110.11868795856711264No Hit
GTCACCGGGAAAAGAAAACCCAAAGCTCTAAGGTAACAAATTACCAAATTAC110.11868795856711264No Hit
GGTACTGGGGGATAGTCCATATGTTTTAATATAATTCTATATTGCGGACACA110.11868795856711264No Hit
CATTGCGGGAAGGCAGCGGTGCTTAATCAATATTTCCTGTGCTCACCAGAGG110.11868795856711264No Hit
ACAGGGGGTGGTCAAAGTGCTGCTCGACTGCGGGGCCGACCCGGACAGCAGG110.11868795856711264No Hit
AATGCGGGGTGGACTACAGATTGGGAATGGATGTGGGTTACAAGTTGGGAAC110.11868795856711264No Hit
AATCGAGGGAGAAACTCACCTGTTCCCCATCATACTTTCATCCCAGAGGTTT110.11868795856711264No Hit
CAGTGTGGGCATAACCTCCAGCAGGCATTTCATTTAATACTGAGCTTAGTTC110.11868795856711264No Hit
AGTTTAGGGGTGGAAGGTGTCAATTTGGTTTAGAATGCTGCGCTTACCTTCC110.11868795856711264No Hit
GTCTGTGGTGGGATGGAATGGCCATGGCATTTTTTTCAGAGAACATCCTTTA110.11868795856711264No Hit
CTTCTAGGGTGCCAGTACAGCCCTACTGCATCATCTGCGTCAGCCGGTCCTA110.11868795856711264No Hit
AACGCGGGAATGTGGGCTTCAATGTCAAGAATGTGTCTGTCAAGGATGTTCG110.11868795856711264No Hit
GTCGAGGGGTCTGCGAAGGGAAGATGGAAGGAGACCATGAACCGAGGGGTTT110.11868795856711264No Hit
CTTAGTGGGATGCTTTATACTGCCGAGTCTGGGGGCTTGTTTTGGTTTGGGG110.11868795856711264No Hit
CTGCCTGGGATCTCTTAATACTGTTGCATTGGGGATTAGGTTTCAATATATG110.11868795856711264No Hit
GTGCCCGGGGGGGCTCATGGATTGGCCCCGACCACGACAAGTTCAGTGCCAT100.10789814415192059No Hit
TTAGGTGGGGCCAGTAACTATTAATATCTAGTTCTCAGAGCATTTGGAAAGG100.10789814415192059No Hit
GACTATGGGACTTTTTCATATTATTATTAAACTACCACAGCTCTTTAAAAGC100.10789814415192059No Hit
CAATGGGATGATGGAATTAAAGTTGATGAGGGTGAACTTTGAGCATTAATAG100.10789814415192059No Hit
TTAGCAGGGGACCAAACCATTTGTATCTGGCATCACTTACTAACACACGACA100.10789814415192059No Hit
ACAAGTAGGGTATGAGTCAACTGCCAAGGGCTTTCAATTATGTCTACTAGAG100.10789814415192059No Hit
TGCGTGGGGCATTATATGAGATGGTGACATTAGAGGAATTCTTTTTTAGTAT100.10789814415192059No Hit
CATGGGGATGATATCTCATTGTGGTTTTGATTTGCATTTCCCTGATTAGTGA100.10789814415192059No Hit
TAACATGGGAAGTCAAAATTATCAAAGATAGAGTGTCGTACAATAAAAATTG100.10789814415192059No Hit
GTAGCTGGGGCTCTGGTGTAGACCATGGACCAAAAGAACTTCTTAGAGTCTG100.10789814415192059No Hit
TCATAAGGGATGTGTGATTTCATCTAAGAGATATATACATGTACACATGCCC100.10789814415192059No Hit
GTGGCGGGCACCTGTAGTCCCAGCTACTCGGGAAGCTGAGGCAGGAGAATGG100.10789814415192059No Hit
TTTGCTGGGAATATAAAAATTCTCTTTCATTTCTTTTTGCCAGCAATCAAAA100.10789814415192059No Hit
TTTATGGGGGAATGAAAGAATAAATGCGTGCATTCCATAGGATGCAAGAAAA100.10789814415192059No Hit
TAGCAGGGGACCTTTAGTACATCTCTCTACCAAAAAATCTTCCTATCTCTAG100.10789814415192059No Hit
ACTAGAGGGGATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTT100.10789814415192059No Hit
TAGTTGGGTCTGACCGTGGGGGGGAGGAAGTCACAGAAACTGCAGGTGAAGG100.10789814415192059No Hit
ATGACAGGGTGCATCAGAGATTTAATTTTTTTTTAATTTTTAATTTTTTTAA100.10789814415192059No Hit
AGAGCCGGGAGGCCTGGTGTAGGCGCCAGGGGCTCCACAGCTTCCAATCCCC100.10789814415192059No Hit
TCAGACAGGGATATTTCATAAACATTGCATTAGTAGTCCAAATTCAGAATTT100.10789814415192059No Hit
GCTTGGGACAAATAGTTTTTTGTTTACGATTTATGACAGGTTTATGTTACTT100.10789814415192059No Hit
TCTCAAGGGGCAGGCAATTGTGCGTGTGTTCCCAAAGCAGCTTACCCACAGC100.10789814415192059No Hit
CTCCGCGGGCCACATAAGATTCTGACATTTAAACTATGTTTCTTCCATTCTG100.10789814415192059No Hit
ATAAATGGGGTGTGTGTGCGTCTGTGTGCGCGTGTGTGTGCGCGTCTGTGTG100.10789814415192059No Hit
TATGATGGGATGTCCCACTGGGCACACATTGTCATTGATAAACATCTTAACA100.10789814415192059No Hit
TGCAGTGGGAGAGCTGAGTAGCTCAGAAGCCTGACCTGGCCCACAAAACTGA100.10789814415192059No Hit
AGCCTGGGTGACAGAGTGAGACCCTGTCTCAAAAACAACAAAAATCTCAGTG100.10789814415192059No Hit
GTAATGGGGAAACATTTAAATAACTAATACCAATATTTCTGAAACTCTTCCA100.10789814415192059No Hit
GCGCGGGTGAAGGGCTGGAATGCTGGCTGGCCACTCTCTGCCTCAGTGGCCT100.10789814415192059No Hit
AAAGTAGGGATAATTCCACAGCTAGCCATGCAATTATTAGCTTCTTCCTTAA100.10789814415192059No Hit
TCAAGGGGGGTAGTGGAAAAATACATAACACTGCAGCTTCTCCTTAGTGGTT100.10789814415192059No Hit
CCCGCCAGGTCCCTCCTCCAACACTGGGATTACAGTTTGACATGAGATTTGG100.10789814415192059No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)