FastQCFastQC Report
Sat 31 Dec 2022
SRR5846590.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846590.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167785
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGTATGC47622.838155973418363No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGTATGCCGTCT37082.2099710939595316No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCT8970.5346127484578478No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTA5020.2991924188693864No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCCTGTCTCTTATAC3300.1966802753523855No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTAT2910.17343624281073994No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCCTGTCTCT2690.16032422445391423No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGT2670.1591322227851119No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGTATGCC2440.14542420359388503No Hit
CGCAACGGGGATTATAAACTCTATTTTGAGGAGATGCCTTCTGTCGACTAAG1870.11145215603301845No Hit
AAGCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTC1870.11145215603301845No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTAACTCGTATGC1750.10430014602020442No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)