FastQCFastQC Report
Sat 31 Dec 2022
SRR5846589.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846589.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166650
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGTATGC46122.7674767476747677No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGTATGCCGTCT37082.225022502250225No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCT9370.5622562256225623No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTA4790.28742874287428744No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCCTGTCTCTTATAC3290.19741974197419743No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGT2950.177017701770177No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTAT2870.17221722172217221No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCCTGTCTCT2860.1716171617161716No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGTATGCC2150.12901290129012902No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTAACTCGTATGC1700.10201020102010201No Hit
AAGCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTC1670.1002100210021002No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)