FastQCFastQC Report
Sat 31 Dec 2022
SRR5846586.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846586.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154369
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGTATGC45562.951369769837208No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGTATGCCGTCT44522.8839987303150245No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCT9260.599861371130214No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTA5580.36147153897479417No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCCTGTCTCTTATAC3470.2247860645595942No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTAT2840.18397476177211747No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCCTGTCTCT2550.16518860652073927No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGTATGCC2110.13668547441519993No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGT2070.13409428058742365No Hit
AAGCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTC1700.11012573768049284No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTAACTCGTATGC1640.10623894693882838No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGT1630.10559114848188432No Hit
AGCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCG1550.10040876082633171No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)