FastQCFastQC Report
Sat 31 Dec 2022
SRR5846585.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846585.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155295
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGTATGC46352.984642132715155No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGTATGCCGTCT44082.838468720821662No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCT8840.569239189928845No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTA5960.38378569818732094No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCCTGTCTCTTATAC3500.225377507324769No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCCTGTCTCT2750.17708232718374706No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTAT2640.1699990340963972No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGT2340.1506809620399884No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGTATGCC1960.12621140410187065No Hit
AAGCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTC1950.125567468366657No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTAACTCGTATGC1920.12363566116101612No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTTACCTATCTCGT1590.1023857818989665No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)